Objective:To investigate the correlation between intraoperative red blood cell (RBC) transfusion and postoperative continuous renal replacement therapy (CRRT) in patients with acute type A aortic dissection (ATAAD).Methods:Clinical data of 302 patients undergoing emergency surgery for ATAAD in the Department of Cardiac Surgery at Beijing Anzhen Hospital from September 2015 to October 2020 were retrospectively analyzed. Among them, 238 were males with a nean age of(46.3±10.0) years old; 64 were females with a mean age of (52.0±11.7) years old. Patients were divided into the CRRT group and the non-CRRT group based on whether they received CRRT postoperatively. Logistic regression analysis was used to investigate the correlation between intraoperative RBC transfusion and postoperative CRRT. Results:Forty-two patients (13.9%) required CRRT postoperatively. Univariate logistic regression analysis showed that intraoperative RBC transfusion increased the risk of postoperative CRRT ( OR=2.499, 95% CI: 1.266-4.470, P=0.008). However, after adjusting for other variables, multivariate logistic regression analysis showed that the association was not statistically significant ( OR=1.777, 95% CI: 0.583-5.417, P=0.312). Conclusion:The increased risk of postoperative CRRT in ATAAD patients due to intraoperative RBC transfusion is caused by confounding factors, and they are actually not significantly correlated.

Objective:To investigate the correlation between intraoperative red blood cell (RBC) transfusion and postoperative continuous renal replacement therapy (CRRT) in patients with acute type A aortic dissection (ATAAD).Methods:Clinical data of 302 patients undergoing emergency surgery for ATAAD in the Department of Cardiac Surgery at Beijing Anzhen Hospital from September 2015 to October 2020 were retrospectively analyzed. Among them, 238 were males with a nean age of(46.3±10.0) years old; 64 were females with a mean age of (52.0±11.7) years old. Patients were divided into the CRRT group and the non-CRRT group based on whether they received CRRT postoperatively. Logistic regression analysis was used to investigate the correlation between intraoperative RBC transfusion and postoperative CRRT. Results:Forty-two patients (13.9%) required CRRT postoperatively. Univariate logistic regression analysis showed that intraoperative RBC transfusion increased the risk of postoperative CRRT ( OR=2.499, 95% CI: 1.266-4.470, P=0.008). However, after adjusting for other variables, multivariate logistic regression analysis showed that the association was not statistically significant ( OR=1.777, 95% CI: 0.583-5.417, P=0.312). Conclusion:The increased risk of postoperative CRRT in ATAAD patients due to intraoperative RBC transfusion is caused by confounding factors, and they are actually not significantly correlated.

Objective: To investigate the effects of retinoid-related orphan receptor γ (RORγ) gene on proliferation and metastasis of human colon cancer cells． Methods: RORγ knockdown cell lines were constructed and the knockdown efficiency was detected by RT-qPCR and Western blot assays ; MTT，colony formation，Transwell and wound healing assays were used to detect cell proliferation and metastasis ; the expression of epithelial-mesenchymal transition (EMT) related proteins was detected by Western blot．The relationship between RORγ gene expression and immune cell infiltration in tumor microenvironment was analyzed using TIMER 2. 0 database. Results : The knockdown of RORγ enhanced the viability (F = 157. 40，P＜0. 01) ，clonogenesis (F = 61. 35，P＜0. 01) ，migration (F = 13. 00，P＜0. 01) ，invasion (F = 21. 26，P＜0. 01) and wound healing ability (F = 877. 2，P＜0. 01) of colon cancer cells，inhibited the expression of E-Cadherin，and promoted the expression of vimentin and N-Cadherin．TIMER 2. 0 database analysis showed that RORγ expression in colon adenocarcinoma ( COAD) tissues was associated with multiple immune cell infiltrates． Conclusion Downregulation of RORγ expression promoted the proliferation and metastasis of colon cancer cells.

Objective To summarize the treatment experience and strategies of patients with acute type A aortic dissection involving coronary arteries in order to improve the efficacy of such patients.Methods Between March 2013 and April 2016,we recruited 37 patients with coronary involvement caused by acute type A aortic dissection,26 men,11 women;mean age (49.7 ± 10.4) years.All procedures were done on an emergency basis within 24 hours after the patient's arrival.Results Acute type A aortic dissection with coronary involvement is a more complex operation associated with high in-hospital mortality(18.9％,7/37) and low short-term survival(64.9％,13/37).There were 9 patients underwent coronary artery bypass graft after completion of the root procedure because of ventricular wall motion abnormality(7 patients) and new ST-segment elevation (2 patients) during weaning from cardiopulmonary bypass.Four of them were survival during follow-up due to the success from rescue coronary artery bypass graft.Conclusion Acute type A aortic dissection with coronary involvement is associated with high in-hospital mortality and low short-term survival.If patients suffered abnormal ventricular wall motion or new ST-segment elevation during weaning from cardiopulmonary bypass,rescue coronary artery bypass graft is essential to salvage these critically ill patients.