Abstract: Objective To investigate the clinical distribution characteristics, drug resistance trends and the carrying of antiseptic resistance gene of Pseudomonas aeruginosa infection in children in Suzhou, in order to provide theoretical basis for the prevention and treatment of Pseudomonas aeruginosa infection in children. Methods The clinical distribution characteristics and drug resistance trends of Pseudomonas aeruginosa isolated from Children's Hospital of Soochow University from 2016 to 2021 were retrospectively analyzed. Forthermore, 101 strains of Pseudomonas aeruginosa were randomly selected to detect the expression of 9 antiseptic resistance genes (qacEΔ1-sul1, qacE, qacEΔ1, qacG, sugE(p), sugE©, emrE, ydgE, ydgF) by polymerase chain reaction. Results Pseudomonas aeruginosa in Soochow University Children's Hospital was mainly isolated from respiratory specimen (47.83%), pus (28.60%) and urine (11.72%); the main departments were intensive care unit(21.45%), general surgery department (15.71%) and respiratory department (12.31%). Patients were mainly aged from 1 month to 1 year old and older than 6 years old (34.31% and 25.38%). The top three drug resistance rates of Pseudomonas aeruginosa were imipenem (11.25%), aztreonam (9.26%) and meropenem (8.02%). Among the 853 strains of Pseudomonas aeruginosa, the drug-resistant strains were mainly from the intensive care unit (58/183), hematology department (33/91), neonatology department (31/96), and there were 57 strains of multi-drug-resistant strains with the detection rate of 6.68%. There were 98 strains (11.49%) of Carbapenem resistant Pseudomonas aeruginosa, and the annual detection rates were 22.06%, 8.40%, 3.60%, 5.67%, 9.85% and 17.20%, respectively. Among the 9 antiseptic resistance genes, the carrying rate of ydgF, sugE© and qacE was 98.02%, 94.06% and 0 respectively. Conclusion Pseudomonas aeruginosa has high resistance to some drugs, so attention should be paid to rational drug use. The carriage rates of of two antiseptic resistance genes exceeded 90%, indicating the need to strengthen research on the mechanism of antiseptic resistance research and rational use of disinfectants

Humans ; Laryngeal Neoplasms ; radiotherapy ; Radiation Tolerance ; Radiotherapy

Objective To explore the relationship between the clinical features and the bacteria quantity confirmed by semi-quantitative culture of sputum in hospitalized infants with wheezing diseases.Methods Nine hundred and fifty-seven patients with wheezing diseases were enrolled from Jan.1,2010 to Dec.3 1,2011,and their clinical characteristics were collected.Respiratory seeretions were collected on admission by the pathogenic examination.They were grouped into 3 groups by the bacteria quantity,and the predictive analytics statistical saftware 20.0 was used to analyze the relationship between the clinical features and the bacteria quantity confirmed by semi-quantitative culture of sputum.Results 1.A total of 372(38.87％,372/957 cases)patients had positive sputum cuhures,and there were 585 patients in 0 + group,225 patients in 1 +-2 + group,147 patients in 3 +-4 + group;the main pathogens in 1 +-2 + group were haemophilus influenza (24.89％,56/225 cases) and streptococcus pneumonia (16.00％,36/225 cases) ;the main pathogens in 3 +-4 + group were streptococcus pneumonia(54.42％,80/147 cases) and haemophilus influenza(8.84％,13/147 cases).2.Presence of siblings,cyanosis,neutrophils and C-reactive protein were higher in 3 +-4 + group when compared with those of 0 + group,shortness of breath was common in 1 +-2 + group.3.Multinomial Logistic regression analysis identified the presence of siblings,residence,passive smoking,course of disease and cyanosis were identified as risk factors in 3 +-4 + group; gender,fever peak ＞ 38.5 ℃,breast-feeding,passive smoking,age,course of disease and cyanosis were as risk factors in 1 +-2 + group.4.Severe pneumonia was common in 3 +-4 + group.Conclusions Bacterial are often detected in infants hospitaled with wheezy episodes,and bacteria quantity is correlated with clinical presentation.Severe pneumonia is common in higher bacteria quantity group.

Objective To evaluate the clinical effect of salmeterol xinafoate and fluticasone propionate powder for inhalation combined ipratropium bromide aerosol in patients with stable chronic obstructive pulmonary disease (COPD).Methods One hundred and twenty patients with stable COPD was randomly divided into control group and observation group with 60 patients each.The control group was received ipratropium bromide aerosol inhalation,and the observation group was given salmeterol xinafoate and fluticasone propionate powder for inhalation and ipratropium bromide aerosol for 6 months.BODE index,SGRQ scores,plasma cortisol and bone mineral density were compared between two groups before and after treatment.Results BODE index scores had no significant difference between two groups before treatment (P＞0.05).After treatment,BODE index scores,dyspnea scale,FEV1％,6MWD and BODE index total scores in observation group were superior to those in control group [ (1.9 ± 0.5) grades vs. (2.3 ± 0.5)grades,(58.5 ± 7.3)％ vs.(50.4 ± 6.2)％,(411.1 ± 56.8) m vs.(347.5 ± 60.4) m,(3.3 ± 1.0) scores vs.(3.8 ± 1.3 ) scores ],there were significant differences (P＜0.05 ).SGRQ scores had no significant difference between two groups before treatment (P＞0.05 ).After treatment and in SGRQ scores,respiration symptom,limitation of activity,disease influence scores in observation group were significantly lower than those in control group [ (20.7 ± 10.3 ) scores vs.(37.9 ± 14.4) scores,(20.7 ± 9.5 ) scores vs.(34.8 ± 13.0) scores,(16.3 ± 6.7) scores vs.(27.2 ± 11.8) scores,(17.5 ± 7.0) scores vs. (34.6 ± 12.3) scores],there were signiticant differences (P＜0.01 ).There were no significant difference of plasma cortisol and bone mineral density between two groups (P＞0.05).Conclusions Salmeterol xinafoate and fluticasone propionate powder for inhalation and ipratropium bromide aerosol in patients with stable COPD can greatly improve the lung function and life quality and with less adverse reaction.

Objective To evaluate the clinical effect and influential factors of intrauterine device (IUD) after insertion under B-type ultrasound guidance. Methods A total of 434 healthy women of childbearing potential, aged for 18 to 40 years were enrolled. After IUD insertion under ultrasound guidance, the uterine profile was observed during the procedure and the patients’ biological characteristic was recorded and gynecologic examiation was performed at month 1, 3, 6, 12, 18 during following up. All data were analyzed with SPSS software. Results There was no accident in operation.In 18 months after IUD insertion, the pregnancy rate with IUD was 1.94%,the expulsion rate was 4.59% and the removal rate was 5.54%. The risk factors of expulsion were the history with cesarean delivery and artificial abortion plus IUD insertion (P

OBJECTIVETo observe reversal of ventricular remodeling by the protein kinase C inhibitor Scutellarein in spontaneously hypertensive rats (SHRs).

METHODSTwelve SHRs were randomly divided into two groups. Scutellarein and saline (10 mg x kg(-1) x d(-1)) were given by intraperitoneal injection to two groups of rats separately. Systolic blood pressure (SBP) and ventricular weight index (LVW/BW, RVW/BW) were measured. A polarization microscope and an image analyzer system (IAS) were used to observe changes in cardiovascular structure and to count the content of cardiac muscle interstitial collagen.

RESULTSThe pathologic changes in the left ventricle in the Scutellarein group rats (SHR(D)) improved to varying degrees, including hypertrophy of the cardiac muscle and collagen volume fraction.

CONCLUSIONScutellarein can reverse ventricular remodeling, improve myocardial stiffness and protect heart cardiac muscle.

Animals ; Female ; Flavanones ; Flavonoids ; pharmacology ; Hypertension ; physiopathology ; Male ; Protective Agents ; pharmacology ; Protein Kinase C ; antagonists & inhibitors ; Random Allocation ; Rats ; Rats, Inbred SHR ; Ventricular Remodeling ; drug effects

ObjectTo study the effect of polydatin (PD) and asymmetric dimethylarginine (ADMA) on the endothelial function in aorta vascular strips of healthy rabbits and the interaction between PD and ADMA. MethodsDose-response curves of phenylephrine (PE) on the aortic strips with or without ADMA and/or PD. E max and Kd from PE were obtained and compared. ResultsNormal aortic strips could not respond to ADMA. Pretreatment with either PD or ADMA had no effect on the contractive response of aortic strips to PE. But PD could significantly weaken the contractive response of aortic strips pretreated with ADMA caused by PE in a dose-dependent manner, increase Kd and decrease E max of PE. ConclusionEither ADMA or PD alone does not influence the normal aortic strip contractive functions, the PE affinity to ?-receptor, and E max. But PD could noncompetitively antagonize the contractive reaction of aortic strips to PE in the presence of ADMA.

Ocular signs and serum vitamin A concentration are commonly used tor the diagnosis of vitamin A deficiency. Recently, impression cytology was suggested as a method for early detection of vitamin A status. Twenty patients with normal eyes and digestive disease, and 111 children with normal eyes were evaluated by serum vitamin A concentration, relative-dose-response and conjunctival impression cytology test. About 90% of the patients with normal conjunctival impression cytology has normal vitamin A status. About 70% of the patients with abnormal conjunctival impression cytology has abnormal vitamin A status despite their seemingly normal clinical ocular examination, and so did the children. These results suggest that impression cytology can identify individuals with preclinical vitamin A deficiency.

Effects of different edible oils on lipid and lipld peroxidation and their correlation were studied in rats. The results showed: 1.The tea-seed oil and soybean oil exhibited a good potency of reducing serum TC and TG comparing with lard did. 2. The plasma LCAT activity in the lard feeding group (57.07 nmol/ml.h) was lower than that of groups consuming four different vegetable oils which produced similar LCAT activities (80.26-94.03 nmol/ml-h). 3. Lard increased liver lipid and promoted TG sediment in aorta, but lowered brain lipid level. 4. Of lipid peroxidation in serum, liver, heart and kidney, the rats fed lard exhibited a highest level, and tea-seed oil fed group was lower relatively soybean oil promoted tissue lipid peroxidation while rape-seed oil and Salad oil suppressed it. 5. After experiment, the SOD activity of erythrocyte increased in rats fed oils riched in monounsaturated fatty acid, and decreased in rats fed soybean oil and lard. 6. The results of linear correlation and regression analysis showed that there was a significant positive relationship between lipid peroxidation and lipid TC, TG) in serum and liver (P

Objective: To study the radioprotective effect of flavonoids from Ginkgo biloba leaves(GBF). Methods: Three water extracts of GBF were prepared (low dosage 10 mg/100 ml, medium dosage 20 mg/100 ml and high dosage 100 mg/100 ml) and orally administered to mice . After 10 d, the mice were exposed to 8.5Gy -rays. After another 10 d of oral administration, the survival rates were recorded in 30 d. In another experiment, six groups of mice (three GBF groups, radiation control, normal control and cyclophosphamide group) were arranged. The first three groups were orally administered with low, medium and high dosage of GBF respectively for 11d; the other three groups with distilled water. Then the three GBF groups and radiation group were exposed to 1.0Gy -rays. Then they were orally administered again in the following 7d . Micronucleated polychromatic erythrocytes in bone-marrow and sperms (AFS) in mice were observed on the 21st day after termination of oral administration. Proliferation rates of lymphocyte (PRL) were determined in the three GBF groups and normal control. Results: Low, medium and high dosage of GBF increased the survival rates by 31.7%, 25.3% and 26.5% respectively(P