Objective To study the effect of interleukin-15(IL-15)on lipopolysaccharide(LPS)-induced apoptosis of septic cardiomyocytes and its related mechanism.Methods H9C2 cells were divided into control group(H9C2 cells in conventional culture),apoptosis group(LPS-induced myocardial cell injury model was established),and intervention group(10 ng/ml recombinant rat IL-15 was used in myocardial H9C2 cell injury model for 6 h).The effects of IL-15 on H9C2 cell proliferation and apoptosis were detected by methyl thiazolyl tetrazolium(MTT)assay and flow cytometry,the level of mitochondrial membrane potential was detected by staining of tetramethylrhodamine ethyl ester(TMRE).The sepsis mouse model was established by intraperitoneal injection of 10 mg/kg LPS,and 100 μl of normal saline containing 100 μg/ml IL-15 was injected intraperitoneally.HE staining was used to evaluate the pathological injury of mouse myocardium.The indexes of oxidative stress in mouse myocardium were detected by ELISA.The effects of IL-15 on the expression of heme oxygenase 1(HO-1)and NF-E2-related factor 2(Nrf2)mRNAs and proteins in myocardium of mouse sepsis model were detected by real-time fluorescence quantitative PCR and Western bloting.Results The apoptosis rate of H9C2 cells in the apoptosis group was significantly higher than that in the control group(47.9%±5.1%vs.2.1%±0.3%,P<0.05).The apoptosis rate of H9C2 cells in the intervention group was 20.7%±2.7%,which was significantly lower than that in the apoptosis group(P<0.05).LPS could significantly inhibit the proliferation of myocardial cells,while IL-15 could inhibit LPS-induced decrease in myocardial cell viability(P<0.05).The relative mitochondrial membrane potential of myocardial H9C2 cells in the apoptosis group was significantly lower than that in the control group(2.6±4.6 vs.7.4±5.2,P<0.05).The relative mitochondrial membrane potential of myocardial H9C2 cells in the intervention group was 4.2±4.9,which was significantly higher than that in the apoptosis group(P<0.05).LPS significantly enhanced the loss of mitochondrial membrane potential of myocardial H9C2 cells,and IL-15 intervention weakened the loss of mitochondrial membrane potential of H9C2 cells induced by LPS(P<0.05).HE staining revealed obvious myocardial tissue damage and inflammatory cell infiltration in mouse sepsis model.IL-15 ameliorated LPS-induced myocardial injury in mice.The expression levels of superoxide dismutase(SOD)and total antioxidant capacity(T-AOC)in myocardium of sepsis mice were significantly lower than those in normal mice,while the expression level of malondialdehyde(MDA)in sepsis mice was significantly higher than that in normal mice(P<0.05).After IL-15 intervention,the expression of SOD and T-AOC in myocardium of sepsis mice was increased,and the expression of MDA was decreased(P<0.05).The mRNA and protein expression levels of HO-1 and Nrf2 were low in myocardium of normal mice.The mRNA and protein expression levels of HO-1 and Nrf2 in myocardium of sepsis mice were significantly higher than those in normal mice(P<0.05).After IL-15 intervention,the mRNA and protein expression levels of HO-1 and Nrf2 were significantly increased in myocardium of sepsis mice(P<0.05).Conclusion IL-15 can protect cardiomyocyte apoptosis induced by sepsis and decrease myocardial injury in sepsis mice.The mechanism may be related to the activation of HO-1/Nrf2 signaling pathway and the inhibition of myocardial oxidative stress in septic mice.

Objective:To explore the incidence, diagnosis and treatment of retinopathy of prematurity(ROP) in preterm infants born in the Guangdong Women and Children′s Hospital and transported from other hospital.Method:s Clinical data of 755 premature infants with ROP at Neonatal Intensive Care Unit, Guangdong Women and Children′s Hospital from January 2013 to December 2015 were retrospectively analyzed.There were 239 cases born in the hospital and 516 cases transported from other hospitals.Their gestational age, birth weight, gender, severity of ROP lesion and clinical data were collected and compared.Result:s The birth weight in the group of transported from other hospital was lower than that in the group of born in the hospital[(1 290.64±392.87) g vs.(1 586.21±512.74) g], and the difference was statistically significant( P<0.001). The ROP diagnosis of gestational age in the group of transported from other hospital was higher than that in the group of born in the hospital[(35.53±2.81)weeks vs.(34.51±2.17)weeks], and the difference was statistically significant( P<0.001). On the proportion of severe condition [such as lesion area Ⅰ, aggressive posterior retinopathy of prematurity(AP-ROP) and plus combined lesions], in the group of transported from other hospital was higher than that in the group of born in the hospital, and the differences was statistically significant( P<0.001). In the comparison of the proportion of laser photocoagulation, vitreous injection, combination of the two operations and supplementary laser therapy, in the group of transported from other hospital were higher than those in the group of born in the hospital[60.1%(310/516 cases) vs.20.9%(50/239 cases); 10.9%(56/516 cases) vs.2.5%(6/239 cases); 8.1%(42/516 cases) vs.1.7%(4/239 cases); 4.5%(23/516 cases) vs.1.3%(3/239 cases)], and the differences were statistically significant(all P<0.001). Conclusions:Premature infants with ROP transported from other hospitals have lower birth weight, severe ROP lesions and high surgical intervention rate.Improving ROP screening level in primary hospitals, timely diagnosis and efficient transportation can help to effectively prevent the deterioration of ROP in premature infants and improve their quality of life.

Objective:To review the clinicopathological characteristics and analyze the prognostic factors of hepatoid adenocarcinoma of stomach(HAS).Methods:From August 2012 to June 2017,30 patients with HAS were diagnosed in Daycare of Peking University Cancer Hospital.Clinicopathological data and follow-up information of these patients were retrospectively collected and analyzed.Re-sults:The median age of these 30 patients was 58 years at diagnosis,with a male-to-female ratio of 2.75:1.Twenty-nine patients were confirmed to have lymph node metastases and 7 patients had distant metastases.Ten patients died because of the cancer within the follow-up period.The 1-and 3-year survival rates were 60% and 52%,respectively.Cox multivariate regression revealed that elevated serum CA199 levels,higher lymph node staging,not having undergone radical surgery,and stronger immunohistochemical(IHC)stain-ing intensity of alpha fetoprotein(AFP)were independent poor prognostic factors.Conclusions:Elevated serum CA199 levels,lymph node staging,and IHC staining intensity of AFP are verified in this study as independent risk factors of poor outcome in HAS patients. Early detection and diagnosis of the disease may improve the clinical prognosis.Multidisciplinary team discussions are important in making therapy decisions and radical surgery should be performed whenever possible.

n-Octanol/ water partition coefficients (Kow ) is an important parameter commonly used to explain toxicity, activity and transmembrane of drugs. However, it is difficult to be detected by direct experimental determination. In this work, a set of 29 neutral and acidic analogues of naphthalene and anthraquinone with reliable experimental Kow data was chosen as model compounds for establishing linear relationship between the logarithm of apparent n-octanol/ water partition coefficient (lgKow), and the logarithm of reversed phase-high performance liquid chromatography (RP-HPLC) retention factor of the solutes corresponding to neat aqueous fraction of mobile phase (lgkw ) as the quantitative structure-retention relationship (QSRR) model. Methanol-water mixture was used as mobile phase at various pH, and retention time (tR ) was rectified by a dual-point retention time correction (DP-RTC) in this method. The experiment results indicated that the proposed QSRR model had good correlation coefficient R2 = 0. 974 -0. 976 with satisfactory results of internal and external validation (the cross-validated correlation coefficient R2cv of 0. 970-0. 973, and 1. 4% ≤relative error (RE)≤7. 9% for all the 6 verification compounds). In addition, this QSRR model was compared with linear solvation energy relationship ( LSER) involved in different descriptors of molecular structure, showing no differences. The QSRR model was applied to measure Kow of 11 naphthalenes and anthraquinones, and the predicted data were compared with Shake-flask method (SFM) experimental ones, as well as calculated ones obtained by software. The results suggested that the proposed method for Kow determination in this work was more accurate, simple and fast. To the best of our knowledge, this is the first report on measuring Kow data for these compounds. The proposed strategy provides the possibility in determining Kow of lipophilic components in complex mixture more quickly and accurately by RP-HPLC.

D-psicose 3-epimerase (DPEase) is demonstrated to be useful in the bioproduction of D-psicose, a rare hexose sugar, from D-fructose, found plenty in nature. Clostridium cellulolyticum H10 has recently been identified as a DPEase that can epimerize D-fructose to yield D-psicose with a much higher conversion rate when compared with the conventionally used DTEase. In this study, the crystal structure of the C. cellulolyticum DPEase was determined. The enzyme assembles into a tetramer and each subunit shows a (β/α)(8) TIM barrel fold with a Mn(2+) metal ion in the active site. Additional crystal structures of the enzyme in complex with substrates/products (D-psicose, D-fructose, D-tagatose and D-sorbose) were also determined. From the complex structures of C. cellulolyticum DPEase with D-psicose and D-fructose, the enzyme has much more interactions with D-psicose than D-fructose by forming more hydrogen bonds between the substrate and the active site residues. Accordingly, based on these ketohexose-bound complex structures, a C3-O3 proton-exchange mechanism for the conversion between D-psicose and D-fructose is proposed here. These results provide a clear idea for the deprotonation/protonation roles of E150 and E244 in catalysis.
Binding Sites ; Biocatalysis ; Catalytic Domain ; Clostridium cellulolyticum ; enzymology ; Hexoses ; chemistry ; Manganese ; chemistry ; Protein Structure, Quaternary ; Racemases and Epimerases ; chemistry ; metabolism ; Substrate Specificity

The fingerprint chromatograms of Arisaematis Rhizoma were established by HPLC. The analysis was performed on a Lichrospher C18 (4.6 mm x 200 mm, 5 microm) column with acetonitrile-water (containing 0.1% acetic acid) as mobile phase at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 270 nm, and the column temperature was 30 degrees C. The similarities of the fingerprint chromatograms were calculated over 0.9 between 11 batches of Arisaematis Rhizoma samples by analyzing 14 common peaks with adenosine as reference substance. However, their fingerprint chromatograms were significantly different from those of Pinellia pedatisecta and P. ternate. Adenine, hypoxanthine, xanthine, uridine, guanosine, adenosine, schaftoside, and isoschaftoside were identified by comparing the retention times and their ultraviolet spectra. The method is repeatable, exclusive and can be used for identification and evaluation of Arisaematis Rhizoma.
Arisaema ; chemistry ; Chromatography ; Drugs, Chinese Herbal ; chemistry ; standards ; Quality Control ; Reproducibility of Results

OBJECTIVETo investigate the flavonoids in Platycladi Cacumen.

METHODThe constituents in Platycladi Cacumen were determined by UPLC-MS. A Waters BEH C18 column (2.1 mm x 150 mm, 1.7 microm) was used with a gradient elution of methanol-water containing 0.2% formic acid. The mass spectrometer equipped with electrospay ionization source was used as defector and operated in data was collected under the negative ion modes.

RESULTEleven constituents were identified.

CONCLUSIONIn this study, the main flavonoids in Platycladi Cacumen were separated by UPLC, and identified through the information of mass number and UV spectra. With the information of MS/MS from flavonoids, the modes of breaking up flavonoids were discussed. It is an accurate and effective method which can be applied for the constituent identification of Platycladi Cacumen.

Chromatography, Liquid ; methods ; Cupressaceae ; chemistry ; Flavonoids ; chemistry ; Mass Spectrometry ; methods ; Plant Extracts ; chemistry

Objective To study the effects of caesarean section on breastfeeding.Methods Divides into 2 groups stochastically 120 example c-section parturient woman,each group of 60 examples,the control group gives obstetrics conventional nursing;The observation group carries on the target-oriented behavior intervention and psychological unblocking by professional nurse to the parturient woman:Uses the pre-natal health education seepage,instructs the parturient woman to feed correctly nurses the skill,promotes the wet-nurse nutrition,dredges intervention measures promptly and so on mammary gland tube.Results The observation group parturient women compare the control group to secrete young timing advance,secrete the young quantity to increase,the early nursing success ratio is high,the difference has the significance (P<0.01).Conclusion Early interventions to parturient after caesarean section is propitious to their health recover and elevate the success rate of early breast feeding.

OBJECTIVE:To identify the medical material Ranunculus ternatus. METHODS: Potassium bromide was used for tabletting.The medical material Ranunculus ternatus and its isogeneric plants were analyzed by using infrared spectrometry.RESULTS:The infrared spectrogram of the medical material Ranunculus ternatus was distinctive and somewhat different from those of its isogeneric plants.CONCLUSION:Fourier transformation infrared spectrometry could be used for the identification of Ranunculus ternatus in that it is simple,rapid,reproducible and specific.

OBJECTIVE:To establish the identification method of Rhizoma Arisaematis and content determination of flavon-oids.METHODS:TLC was used to identify Rhizoma Arisaematis with schaftoside and ischaftoside as reference substances.The content of flavonoids was determined by HPLC.RESULTS:TLC of test sample and that of control substance had same color dots.The linear range of schaftoside and isoschaftoside were 7.925～126.8 ?g?mL-1(r=0.999 9) and 3.996～63.94 ?g?mL-1(r=0.999 7) respectively.Average recoveries were 99.4% for schaftoside (RSD=2.10%,n=9) and 99.52% for isoschaftoside(RSD=2.42%,n=9).CONCLUSION:The method is simple,accurate and reproducible for the quality evaluation of Rhizoma Arisaematis.