Sennoside A (SA), a typical prodrug, exerts its laxative effect only after its transformation into rheinanthrone catalyzed by gut microbial hydrolases and reductases. Hydrolases have been identified, but reductases remain unknown. By linking a photoreactive group to the SA scaffold, we synthesized a photoaffinity probe to covalently label SA reductases and identified SA reductases using activity-based protein profiling (ABPP). From lysates of an active strain, Bifidobacterium pseudocatenulatum (B. pseudocatenulatum), 397 proteins were enriched and subsequently identified using mass spectrometry (MS). Among these proteins, chromate reductase/nicotinamide adenine dinucleotide (NADH) phosphate (NADPH)-dependent flavin mononucleotide (FMN) reductase/oxygen-insensitive NADPH nitroreductase (nfrA) was identified as a potent SA reductase through further bioinformatic analysis and The Universal Protein Resource (UniProt) database screening. We also determined that recombinant nfrA could reduce SA. Our study contributes to further illuminating mechanisms of SA transformation to rheinanthrone and simultaneously offers an effective method to identify gut bacterial reductases.

Objective To explore effects of water extract of radix salviae miltiorrhizae on renal injury in rats exposed to cadmium and its possible mechanism.Methods Twenty male and female healthy Wistar rats,respectively,at age of 6 weeks were randomly divided into blank group (n =8),and model group (n =32) after adaptive feeding for 1 week,given intraperitoneal injection of saline for 5 mL/(kg · d) and intraperitoneal injection of cadmium chloride solution for 2.8 mg/(kg · d).Continuously building for 4 weeks,the general situation of rats was observed,and the rat kidney function and kidney tissue pathological changes were detected.After successful molding,the model group was randomly divided into medicine group,negative and positive and blank control groups.The medicine group was given Salvia miltiorrhiza water extract of high dose of 2.7g/(kg · d),Danshen aqueous extract of low dose 1.35 g/(kg · d) by gavage,positive control group was given sodium selenite for 0.05 mg/kg · d by gavage,the negative and blank control groups were given the same mount of saline for 2.7 mL/(kg · d).After 10 weeks of treatment,serun and urine was collected,and kidney tissue was to be detected.Results Salvia miltiorrhiza water extraction liquid of low and high dose group had less 24 h urine volume than negative control group,and serum superoxide dismutases (SOD) increased significantly,Malondialdehyde (MDA) decreased significantly,total antioxidant capacity (T-AOC) increased significantly,blood urea nitrogen,urinary beta β2-MG content and serum creatinine levels was decreased.Pathological section revealed that renal proximal convoluted tubules epithelial cells was turbid,swelling,degeneration and necrosis in the negative control group.Salvia miltiorrhiza water extraction liquid of low and high dose group had lower serum cortical cadmium content than the negative control group,Salvia miltiorrhiza water extraction liquid of low and high dose group had lower apoptosis rate than the negative control group.Conclusion The salvia miltiorrhiza water solution can accelerate the metabolism of cadmium,and effectively interfere with cadmium induced renal injury,delay chronic renal failure,and protect the kidney.

Objective To explore effects of water extract of radix salviae miltiorrhizae on renal injury in rats exposed to cadmium and its possible mechanism.Methods Twenty male and female healthy Wistar rats,respectively,at age of 6 weeks were randomly divided into blank group (n =8),and model group (n =32) after adaptive feeding for 1 week,given intraperitoneal injection of saline for 5 mL/(kg · d) and intraperitoneal injection of cadmium chloride solution for 2.8 mg/(kg · d).Continuously building for 4 weeks,the general situation of rats was observed,and the rat kidney function and kidney tissue pathological changes were detected.After successful molding,the model group was randomly divided into medicine group,negative and positive and blank control groups.The medicine group was given Salvia miltiorrhiza water extract of high dose of 2.7g/(kg · d),Danshen aqueous extract of low dose 1.35 g/(kg · d) by gavage,positive control group was given sodium selenite for 0.05 mg/kg · d by gavage,the negative and blank control groups were given the same mount of saline for 2.7 mL/(kg · d).After 10 weeks of treatment,serun and urine was collected,and kidney tissue was to be detected.Results Salvia miltiorrhiza water extraction liquid of low and high dose group had less 24 h urine volume than negative control group,and serum superoxide dismutases (SOD) increased significantly,Malondialdehyde (MDA) decreased significantly,total antioxidant capacity (T-AOC) increased significantly,blood urea nitrogen,urinary beta β2-MG content and serum creatinine levels was decreased.Pathological section revealed that renal proximal convoluted tubules epithelial cells was turbid,swelling,degeneration and necrosis in the negative control group.Salvia miltiorrhiza water extraction liquid of low and high dose group had lower serum cortical cadmium content than the negative control group,Salvia miltiorrhiza water extraction liquid of low and high dose group had lower apoptosis rate than the negative control group.Conclusion The salvia miltiorrhiza water solution can accelerate the metabolism of cadmium,and effectively interfere with cadmium induced renal injury,delay chronic renal failure,and protect the kidney.

Medical scientific research is the main driving force of development of medical science.In order to ensure the Hospital take the road of sustainable development in a market economy environment,We must rely on technologIcal progress and innovation to improve the comprehensive competitiveness of the hospital.Through analysis the present of our hospital scientific research,Proposed the bottleneck problem how to maintain the momentum of scientific research development.And on this basis investigate the hospital scientific research trends:Achieve major breakthrough in scientific research; To Create scientific For the sake of research team with strong competitiveness,To Strengthen personnel training gradient,reserve new force for development of hospital's scientific research; Apply for the high level scientific research projects,Strengthen the regulatory regime of the scientific research projects; Around a stable long-term scientific research direction to do depth research; Ensure The hospital's scientific research work on a sustainable development path.

ObjectiveTo discuss the influence of community health education on the life style of patients with primary hypertension. MethodsIn the form of lectures, consultation, playing video tape and delivering materials in health education, the community health education concerning psychological nursing, proper food program, timely and reasonable sport, monitoring of blood pressure and timely taking medicine was carried out in 268 patients with hypertension. The effect also was observed using t test and χ2 test. ResultsThe differences between the effect of blood pressure control in patients with hypertension before and after education had statistical significance. The changes of life style before and after education also had statistical significance. ConclusionsAfter the community health education, the patients with primary hypertension will know more about the hypertension, which is beneficial for them to form a healthy life style to improve the effect of blood pressure control.

Objective: To study the effects of pharmaco-serum from rabbits administrated intragastrically with Fuzheng Jiedu decoction(FJD) on injured human bronchial epithelial(16HBE) cells exposed to nickel sulfate(NiSO4).Methods: Cultured 16HBE cells were treated with both NiSO4 and different doses of FJD pharmaco-serum in vitro.The detections of cell activities and viabilities were carried out.Meanwhile,activities of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) as well as maleic dialdehyde(MDA) contents were measured.Results: FJD pharmaco-serum could decrease mortalities and increase viabilities of 16HBE cells exposed to NiSO4.In cells co-treated with NiSO4 and FJD pharmaco-serum,the content of MDA was decreased,while GSH-Px and SOD activities were increased at the same time.High dose of FJD pharmaco-serum had the most dramatic effect.Conclusion: This study suggested that FJD could antagonize NiSO4-induced toxicity,which may be involved in its antioxidant function.