Mitochondrial DNA (mtDNA) acts as a bridge between tissue damage and immune activation, triggering innate and adaptive immune responses through multiple signaling pathways. This review introduces the biological characteristics of mtDNA and its release mechanism in solid organ transplantation-related injuries, discusses the immune responses it mediates, and further explores its relationship with graft damage and rejection in different types of solid organ transplantation. By deeply understanding the biological functions of mtDNA, new ideas are expected to be provided for promoting marginal organ repair, optimizing graft monitoring methods, and developing targeted intervention strategies for transplant-related immune responses.

Objective:To investigate the clinical efficacy of Jiuwei Zhenxin Granules combined with lithium carbonate in improving sleep quality in patients with insomnia during the remission phase of bipolar disorder. Methods:A total of 140 patients with insomnia during the remission phase of bipolar disorder, admitted to Jinhua Maternal and Child Health Care Hospital from March 2021 to March 2024, were selected for this study. This study used a retrospective research method. These patients were divided into a control group ( n = 70) and an observation group ( n = 70) according to the different treatment approaches. The control group received lithium carbonate sustained-release tablets, while the observation group received Jiuwei Zhenxin Granules in addition to the lithium carbonate treatment. The efficacy of the treatments for insomnia during the remission phase of bipolar disorder, as well as sleep quality and psychological status, was assessed. Polysomnography indicators of sleep quality were monitored. Serum levels of sleep-related neurotransmitters were measured. Adverse drug reactions were statistically analyzed. Results:In the observation group, the overall treatment efficacy for insomnia during the remission phase of bipolar disorder was significantly higher at 91.43% (64/70) compared with the control group at 71.43% (50/70) ( Z = 5.00, P < 0.001). After 4 weeks of treatment, the Pittsburgh Sleep Quality Index score, the Bech-Rafaelsen Mania Scale score, the Self-Rating Depression Scale score, and the Self-Rating Anxiety Scale score in the observation group were (7.62 ± 1.83), (22.07 ± 2.42), (26.35 ± 2.79), and (24.68 ± 2.65), respectively, which were significantly lower than those in the control group [(11.57 ± 2.14), (29.45 ± 3.11), (33.42 ± 3.81), (35.17 ± 3.92), t = 10.52, 12.84, 14.25, 17.34, all P < 0.001]. Within 4 weeks after treatment, the total sleep duration in the observation group was longer than that in the control group [(8.09 ± 1.17) hours vs. (6.37 ± 0.96) hours, P < 0.001], while the sleep latency in the observation group was shorter than that in the control group [(20.71 ± 2.28) minutes vs. (24.39 ± 2.62) minutes, P < 0.001]. Additionally, the number of awakenings was fewer in the observation group than that in the control group [(2.36 ± 0.37) times vs. (3.51 ± 0.42) times, P < 0.001]. After 4 weeks of treatment, the levels of sleep-related neurotransmitters serum serotonin, melatonin, β-endorphin, and γ-aminobutyric acid in the observation group were (0.51 ± 0.12) μg/L, (29.65 ± 3.27) ng/L, (18.49 ± 1.92) μg/L, and (287.45 ± 15.60) μg/L, respectively. These values were significantly higher than those in the control group [(0.43 ± 0.10) μg/L, (22.41 ± 2.54) ng/L, (14.05 ± 1.73) μg/L, and (239.06 ± 12.73) μg/L, t = -6.62, -13.43, -11.67, -18.52, all P < 0.001]. There was no significant difference in the incidence of adverse drug reactions between the observation and control groups [8.57% (6/70) vs. 11.43% (8/70), χ2 = 0.31, P = 0.573]. Conclusions:The combination of Jiuwei Zhenxin Granules and lithium carbonate can improve sleep quality, alleviate insomnia-related symptoms, and enhance overall prognosis in patients with insomnia during the remission phase of bipolar disorder by regulating neuroendocrine function.

In recent years, the incidence of autoimmune diseases is increasing, exerting a profound impact on the quality of people′s life. However, current research on the pathogenesis and therapeutic approaches for these diseases remains limited. T cells play a central role in the initiation and progression of autoimmune diseases. As a crucial component of the body′s immune system, T cells are essential in mediating immune tolerance and maintaining immune homeostasis. Recent insights into the delicate mechanisms and therapeutic potential of the regulation of T cells provide an opportunity to develop advanced strategies for the treatment of autoimmune diseases, which would significantly mitigate the adverse effects associated with conventional immunosuppression and antibody therapies. This article reviews the recent progress in the development of novel therapeutic strategies based on T cells for autoimmune diseases, aiming to provide ideas for further research.

Objective:To investigate the clinical efficacy of Jiuwei Zhenxin Granules combined with lithium carbonate in improving sleep quality in patients with insomnia during the remission phase of bipolar disorder. Methods:A total of 140 patients with insomnia during the remission phase of bipolar disorder, admitted to Jinhua Maternal and Child Health Care Hospital from March 2021 to March 2024, were selected for this study. This study used a retrospective research method. These patients were divided into a control group ( n = 70) and an observation group ( n = 70) according to the different treatment approaches. The control group received lithium carbonate sustained-release tablets, while the observation group received Jiuwei Zhenxin Granules in addition to the lithium carbonate treatment. The efficacy of the treatments for insomnia during the remission phase of bipolar disorder, as well as sleep quality and psychological status, was assessed. Polysomnography indicators of sleep quality were monitored. Serum levels of sleep-related neurotransmitters were measured. Adverse drug reactions were statistically analyzed. Results:In the observation group, the overall treatment efficacy for insomnia during the remission phase of bipolar disorder was significantly higher at 91.43% (64/70) compared with the control group at 71.43% (50/70) ( Z = 5.00, P < 0.001). After 4 weeks of treatment, the Pittsburgh Sleep Quality Index score, the Bech-Rafaelsen Mania Scale score, the Self-Rating Depression Scale score, and the Self-Rating Anxiety Scale score in the observation group were (7.62 ± 1.83), (22.07 ± 2.42), (26.35 ± 2.79), and (24.68 ± 2.65), respectively, which were significantly lower than those in the control group [(11.57 ± 2.14), (29.45 ± 3.11), (33.42 ± 3.81), (35.17 ± 3.92), t = 10.52, 12.84, 14.25, 17.34, all P < 0.001]. Within 4 weeks after treatment, the total sleep duration in the observation group was longer than that in the control group [(8.09 ± 1.17) hours vs. (6.37 ± 0.96) hours, P < 0.001], while the sleep latency in the observation group was shorter than that in the control group [(20.71 ± 2.28) minutes vs. (24.39 ± 2.62) minutes, P < 0.001]. Additionally, the number of awakenings was fewer in the observation group than that in the control group [(2.36 ± 0.37) times vs. (3.51 ± 0.42) times, P < 0.001]. After 4 weeks of treatment, the levels of sleep-related neurotransmitters serum serotonin, melatonin, β-endorphin, and γ-aminobutyric acid in the observation group were (0.51 ± 0.12) μg/L, (29.65 ± 3.27) ng/L, (18.49 ± 1.92) μg/L, and (287.45 ± 15.60) μg/L, respectively. These values were significantly higher than those in the control group [(0.43 ± 0.10) μg/L, (22.41 ± 2.54) ng/L, (14.05 ± 1.73) μg/L, and (239.06 ± 12.73) μg/L, t = -6.62, -13.43, -11.67, -18.52, all P < 0.001]. There was no significant difference in the incidence of adverse drug reactions between the observation and control groups [8.57% (6/70) vs. 11.43% (8/70), χ2 = 0.31, P = 0.573]. Conclusions:The combination of Jiuwei Zhenxin Granules and lithium carbonate can improve sleep quality, alleviate insomnia-related symptoms, and enhance overall prognosis in patients with insomnia during the remission phase of bipolar disorder by regulating neuroendocrine function.

In recent years, the incidence of autoimmune diseases is increasing, exerting a profound impact on the quality of people′s life. However, current research on the pathogenesis and therapeutic approaches for these diseases remains limited. T cells play a central role in the initiation and progression of autoimmune diseases. As a crucial component of the body′s immune system, T cells are essential in mediating immune tolerance and maintaining immune homeostasis. Recent insights into the delicate mechanisms and therapeutic potential of the regulation of T cells provide an opportunity to develop advanced strategies for the treatment of autoimmune diseases, which would significantly mitigate the adverse effects associated with conventional immunosuppression and antibody therapies. This article reviews the recent progress in the development of novel therapeutic strategies based on T cells for autoimmune diseases, aiming to provide ideas for further research.

Objective:To investigate the status quo of pain management for cancer patients in hospice care wards of community health service centers.Methods:The electronic medical records of 373 cancer patients admitted in hospice wards of Kangjian Community Health Center of Xuhui District and Jinshanwei Town Community Health Center of Jinshan District from January 2015 to July 2021 were collected. The characteristics of cancer pain, the use of analgesic drugs, the effects of analgesic drugs and its influencing factors were analyzed.Results:The incidence of cancer pain in 373 patients was 93.0% (347/373), and the proportion of moderate to severe cancer pain was 55.6% (193/347). Analgesics were used in 304 patients, among whom 233 (76.6%) patients used oral analgesics, 297 (97.7%) used on time, 97.6%(285/292) used sustained-release opioids, and 94 (30.9%) used combinedly. Breakout pain occurred in 100 cases (32.9%), all of which was controlled with immediate-release morphine. Cancer pain was not relieved in 132 cases (43.42%), and multivariate logistic regression analysis showed that the pain degree on admission (moderate: OR=3.69, 95 %CI:2.09-6.49; severe: OR=5.52, 95 %CI:2.43-12.53), the presence of burst pain ( OR=3.28, 95 %CI:1.77-6.06), the type of analgesics used (non-steroidal+weak opioids: OR=0.39, 95 %CI:0.20-0.76; nonsteroidal+strong opioids: OR=0.20, 95 %CI:0.08-0.51) and the adverse reactions ( OR=1.92, 95 %CI:1.03-3.60) were the influencing factors of pain relief in cancer pain patients (all P<0.05). Conclusion:The pain of cancer patients admitted to community palliative care wards cannot be ignored. Although most cancer pain patients use analgesic drugs in a standard way, there are still a high proportion of patients whose pain is not controlled. Various factors affect the effect of analgesic treatment.

One hundred and fifty diabetic patients,who received management by community family doctors and hospital consortium (1+1+1) team for at least 1 year,were enrolled from January 2017 to May 2017.After that they continued to receive the management for 1 more year by the team which were trained with TeamSTEPPS (team strategies and tools to enhance performance and patient safety).The management effect before and after TeamSTEPPS training was compared.After the TeamSTEPPS training,the team management ability was improved,so that the compliance of diabetic patients with medical advice,regular examination of blood glucose,regular follow-up assessment,health education,regular exercise and diet control were all better than before,and the differences were statistically significant (all P＜0.01).Fasting blood glucose,postprandial 2 h-blood glucose,glycosylated hemoglobin and LDL-C levels were all lower than before,and the achieved rate of the above indicators were also increased (P＜0.05).It is suggested that application of TeamSTEPPS in team training can effectively enhance the management ability for chronic disease of family doctors and improve compliance and clinical indicators of patients with diabetes under management.

Objective By using mouse orthotopic lung transplant model, we investigated the immune mechanisms of an-ti-CD3 induced lung allograft protection .Our study intends to further dissect the features of lung transplant immunology and to provide a novel therapeutic insight for the clinical application of anti-CD3 mAbs after lung transplantation.Methods Murine orthotopic allogeneic lung transplants were performed in C57BL/6 wild type(WT) mice using major histocompatibility complex (MHC) fully mismatched BALB/c donors.Syngeneic transplants were also performed in WT C57BL/6 mice using C57BL/6 donors.For immunosuppressive therapy, allograft recipients received 50g dose of anti-CD3 by intraperitoneal injection on days 2, 3, 4, 5, 6 and 9 post-operation(n=4).At day 10, histopathologic characteristics and rejection status of the pulmonary grafts were assessed.The severity of acute rejection was graded by the pathological score , and T cell and neutrophil infiltration in the pulmonary grafts was evaluated by immunohistochemical(IHC) staining for CD3 and myeloperoxidase(MPO) respective-ly.Real-time RT-PCR was performed for FoxP3, IL-17A and IFN-γexpression in the pulmonary grafts.The percentage of FoxP3+Treg in total CD4+T lymphocytes from the recipient spleens was analyzed by FACS.Results 10 days after transplan-tation, histopathologic examination demonstrated that there is no apparent acute rejection observed in the pulmonary isografts , whereas allografts from untreated recipients have marked inflammatory cell infiltration and pulmonary parenchyma lesion .IHC staining for CD3 and MPO showed that the allograft-infiltrating cells of perivascular layers are mainly T lymphocytes , and the cells around the small airways are mostly neutrophils .Anti-CD3 treatment significantly alleviated the acute rejection of pulmo-nary allografts, when compared with the untreated group.Real-time RT-PCR showed that the expression levels of IL-17A and IFN-γin allografts were markedly elevated compared to those in isografts, and anti-CD3 increased the expression of FoxP3, and reduced the expression of IL-17A and IFN-γin the pulmonary allografts.FACS analysis of splenocytes showed that the percent-age of Treg in total CD4+T lymphocytes increased significantly in the anti-CD3 treated allograft recipients, as compared with the isograft and untreated allograft recipients.Conclusion Anti-CD3 mAbs may alleviate acute rejection of the pulmonary al-lografts by promoting FoxP3 expression and Treg development.

Objective: To examine the prevalence of depression and anxiety symptoms, and the related factors in family members of schizophrenic patients in a Shanghai community. Methods: From March to May 2017, the family members of 206 schizophrenic patients registered in Xuhui district Kangjian community of Shanghai were enrolled in this cross-sectional survey. The participants were evaluated with Patient Health Questionnaire Self-rating Depression Scale (PHQ-9) and the Anxiety Screening Scale (GAD-7). The factors related to depression and anxiety symptoms were analyzed. Results: The detection rates of depressive symptoms and anxiety symptoms in the family members of schizophrenia patients were 40.3%(83/206) and 37.9%(78/206), respectively.Univariate analysis showed that the relationship with the patient, education levels were the influencing factors of depressive symptoms (χ²=17.045, 9.702; P<0.01); while age, relationship with the patient, education levels were factors related to the occurence of anxiety symptoms (χ²=11.862, 22.042, 15.060; P<0.01). Multivariate regression analysis showed that the parents of patient was more prone to depressive symptoms than spouses (OR=2.861, P<0.01); higher education was more prone to be depressive than primary education (OR=10.071, P<0.01). Age group 18-40 years were more prone to anxiety than the age group ≥66 years (OR=0.060, P<0.05); sibling relationship is more prone to anxiety than spouse relationship (OR=10.018, P<0.01), followed by parental relationship (OR=7.467, P<0.01); higher education was more prone to anxiety than primary education (OR=5.119, P<0.05). Conclusions There is high prevalence of depression and anxiety symptoms in family members of schizophrenic patients, which is related to the relationship with the patient, the education level of family members and other factors.

Objective To evaluate the application of TeamSTEPPS in training of community osteoporosis management team .Methods The TeamSTEPPS was applied for training of osteoporosis management team, and 1 513 residents aged ≥45 year were screened for osteoporosis in Kangjian community from January 2014 to March 2017.The identified patients with osteopenia and osteoporosis were intervened for one year.Cognitive level of the risk factors and symptoms associated with osteoporosis were assessed by questionnaires before and after the intervention .The bone density was examined at baseline and 6 months, 12 months after intervention.The effect of intervention was evaluated .Results A total of 467 patients (30.87%) with osteopenia and 476 patients (31.46%) with osteoporosis were detected .The cognitive levels of osteoporosis risk factors , including low calcium, vitamin D deficiency, lack of exercise, low weight, women menopause, matrilineal family history, smoking, excessive alcohol drinking and coffee drinking, were higher than those before intervention ( χ2＝62.909,78.742,59.974,50.478,29.512, 34.699,6.500,15.779,6.089,all P＜0.05).The awareness rates of symptoms , including lower back pain or body bone pain, shorter height and hunchback , low energy or nonviolent fracture , were also increased (χ2＝59.942,76.220,16.856,all P＜0.01).After intervention, the bone mineral density levels were increased compared to those before intervention (F＝530.28, P＜0.01 for osteopenia group; F＝339.51, P＜0.01 for osteoporosis group ); meanwhile, the differences were also significant between 6 months, 12 months after intervention and before intervention , between 12 months and 6 months after intervention (t＝-0.452,-0.968,-0.516,all P＜0.01 for osteopenia group; t＝-0.530,-1.045,-0.518, all P＜0.01 for osteoporosis group ).Conclusion The community osteoporosis management team trained by TeamSTEPPS can effectively improve the management levels of osteoporosis among residents in the community.