Objective:To explore the expression of nuclear factor-κB (NF-κB) and β-catenin in skin lesions of condyloma acuminatum, and analyze their relationship with clinical prognosis.Methods:A prospective study was conducted to select 96 patients with condyloma acuminatum admitted to the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine from December 2020 to June 2023 as the case group. Another 96 normal tissue samples from patients who underwent external genital plastic surgery or circumcision at the same time in the hospital were taken as the control group. All patients in the case group received 5-aminolevulinic acid photodynamic therapy (ALA-PDT) treatment, and the outcome was achieved at 6 months after follow-up. The case group was divided into recurrent subgroups and non- recurrent subgroups based on whether there was a recurrence. The expression of NF-κB and β-catenin in patients with different prognoses between the case group and the control group were compared. The Phi coefficient was used to analyze the correlation between NF-κB, β-catenin, and condyloma acuminatum. COX regression analysis were used to test the relationship between NF-κB, β-catenin and recurrence, and then receiver operating characteristic (ROC) curve was drawn to analyze the predictive value of NF-κB, β-catenin for recurrence in patients with condyloma acuminatum.Results:The positive rates of NF-κB and β-catenin in the lesion tissues of the case group were higher than those in the control group: 55.21%(53/96) vs. 23.96%(23/96), 56.25%(54/96) vs. 36.46%(35/96), and there were statistical differences ( P<0.05). According to the Phi coefficient test, there was a positive correlation between NF-κB and β-catenin positivity and condyloma acuminatum ( r>0, P<0.05). After 6 months of follow-up, 35 of the 96 patients relapsed, with a recurrence rate of 36.46% (35/96). The positive rates of NF-κB and β-catenin in the recurrent subgroup were significantly higher than those in the non-recurrent subgroup: 85.71%(30/35) vs. 37.70%(23/61), 85.71%(30/35) vs. 39.34%(24/61), and there were statistical differences ( P<0.05). COX regression analysis showed that the positive expression of NF-κB and β-catenin was related to the recurrence of patients with condyloma acuminatum after treatment ( HR = 4.017, 3.898, 95% CI 1.516 - 10.646, 1.470 - 10.336, P<0.05). The ROC curve showed that NF-κB, β-catenin, and their combination had moderate predictive value for recurrence in patients with condyloma acuminatum after treatment. The area under the curve (AUC) was 0.740 and 0.732, respectively. Among them, the combined predictive value was the highest, with an AUC of 0.810 ( P<0.05). Conclusions:The positive rates of NF-κB and β-catenin in the skin lesions of patients with condyloma acuminatum are relatively high, and they have certain predictive value for the recurrence of patients after treatment.

Objective:To investigate the impact of early invasive arterial blood pressure (IBP) monitoring on survival and neurological outcomes in out-of-hospital cardiac arrest (OHCA) patients undergoing extracorporeal cardiopulmonary resuscitation (ECPR).Methods:This retrospective cohort study analyzed 44 OHCA patients receiving ECPR between January 2021 and January 2023. Patients were divided into: Early intervention group : IBP established within 3 min of ECMO initiation; Late intervention group : IBP established after ICU admission. Baseline characteristics, ECMO parameters, and clinical outcomes were compared. Multivariable logistic regression (adjusted for age, initial rhythm, etc.) and Spearman's correlation were used.Results:This study included a total of 44 patients treated with OHCA and ECPR, divided into an early intervention group of 23 cases and a late intervention group of 21 cases. The early intervention group showed significantly higher: Survival to discharge (43.5% vs. 9.5%, P<0.05), Good neurological recovery (CPC 1-2: 34.8% vs. 9.5%, P<0.05).Early intervention independently predicted survival (adjusted OR=18.84, 95% CI:1.97-179.98, P=0.01). Stratified analysis by pH (cutoff 7.0) demonstrated consistent benefits in both pH>7.0 ( aOR=0.392, 95% CI:0.106-0.678) and pH≤7.0 subgroups ( aOR=0.385, 95% CI: 0.075-0.695; interaction P=0.183). Early IBP positively correlated with CPC scores ( ρ=0.40, P=0.007). Conclusions:Early IBP monitoring significantly improves survival and neurological outcomes in OHCA-ECPR patients, supporting its integration into standardized protocols.

Objective Advances in synthetic DNA technology have made it much easier to fake human DNA samples.There are literature reports that fake human DNA can be synthesized by different methods and implanted in the field to confuse the investigation or mislead the trial.Therefore,distinguishing authentic human DNA from synthetic DNA and performing individual identification has become a critical scientific challenge.Methods We define a novel composite genetic marker(methylation-microhaplotype)by combining CpG sites stably hypermethylated or hypomethylated in natural human DNA and nearby immediately adjacent microhaplotype sites.A total of 19 locis were obtained according to the screening criteria,and a composite detection system for methylation-microhaplotypes was established using MPS technology.Random volunteer DNA samples were extracted and synthetic DNA samples were prepared based on whole genome amplification techniques.Population DNA samples were analyzed to evaluate forensic parameters and methylation variability of the methylation-microhaplotype markers.Comparative analyses of human and synthetic DNA were conducted to assess the markers'ability to discriminate between the two and to detect/type both components in mixed mixed samples.Results The composite detection system composed of 19 locis demonstrated high individual identification ability,achieving a cumulative individual identification probability of 0.999 999 999 996 86.12 hypermethylated locis and 7 hypomethylated locis had relatively stable methylation levels in 57 human DNA samples.According to the allele methylation rate(Ram)value,the system can effectively identify natural and synthetic DNA samples.Meanwhile,for mixed DNA samples,the presence of human and synthetic DNA samples can be found and genotyped.Conclusion Methylation-microhaplotype genetic markers,which can discover human DNA and synthetic DNA and can detect the presence and genotyping of them from mixed samples,is a potential useful tool for forensic DNA analysis.

Objective:To identify risk factors for developing infected pancreatic necrosis (IPN) in female hypertriglyceridemia-induced acute pancreatitis (HTG-AP) patients with childbearing age.Methods:A retrospective analysis was conducted on clinical data from 460 female HTG-AP patients with childbearing age admitted to Eastern Theater General Hospital of the Chinese People's Liberation Army between December 2013 and December 2022. Patients were divided into the IPN group ( n=140) and non-IPN group ( n=320) based on the occurrence of IPN. General clinical data, laboratory test results and clinical outcomes were compared between the two groups. Univariate and multivariate logistic regression models were used to analyze the risk factors for IPN in reproductive-age HTG-AP patients. Results:Among the 460 patients, 140 (30.4%) developed IPN. Compared with the non-IPN group, the IPN group had a significantly higher proportion of pregnant patients (30.0% vs 10.9%, P<0.001) and a lower proportion with a history of acute pancreatitis (12.9% vs 31.3%, P<0.001). Laboratory findings showed that patients in the IPN group had lower levels of hemoglobin and albumin, but higher levels of blood urea nitrogen and serum creatinine. Clinical outcomes showed that the IPN group had significantly higher rates of severe acute pancreatitis and mortality compared to the non-IPN group. Additionally, the median length of ICU stay and total hospital stay in the IPN group were significantly longer, at 16.5 days and 32.0 days, respectively. Univariate logistic regression analyses showed that pregnancy, previous history of acute pancreatitis, and levels of hemoglobin, total bilirubin, albumin, blood urea nitrogen and creatinine were significantly associated with IPN. Multivariate logistic regression analysis revealed that pregnancy ( OR=2.617, 95% CI 1.494-4.210, P=0.001), a history of acute pancreatitis ( OR=0.339, 95% CI 0.189-0.711, P=0.002), hemoglobin level ( OR=0.945, 95% CI 0.939-0.987, P<0.001), and blood urea nitrogen level ( OR=1.173, 95% CI 1.056-1.198, P=0.004) were independently associated with the occurrence of IPN. Conclusions:Pregnancy, a first episode of acute pancreatitis, lower hemoglobin levels and higher blood urea nitrogen levels are independent risk factors for IPN in female HTG-AP patients with childbearing age.

Objective:To explore the expression of nuclear factor-κB (NF-κB) and β-catenin in skin lesions of condyloma acuminatum, and analyze their relationship with clinical prognosis.Methods:A prospective study was conducted to select 96 patients with condyloma acuminatum admitted to the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine from December 2020 to June 2023 as the case group. Another 96 normal tissue samples from patients who underwent external genital plastic surgery or circumcision at the same time in the hospital were taken as the control group. All patients in the case group received 5-aminolevulinic acid photodynamic therapy (ALA-PDT) treatment, and the outcome was achieved at 6 months after follow-up. The case group was divided into recurrent subgroups and non- recurrent subgroups based on whether there was a recurrence. The expression of NF-κB and β-catenin in patients with different prognoses between the case group and the control group were compared. The Phi coefficient was used to analyze the correlation between NF-κB, β-catenin, and condyloma acuminatum. COX regression analysis were used to test the relationship between NF-κB, β-catenin and recurrence, and then receiver operating characteristic (ROC) curve was drawn to analyze the predictive value of NF-κB, β-catenin for recurrence in patients with condyloma acuminatum.Results:The positive rates of NF-κB and β-catenin in the lesion tissues of the case group were higher than those in the control group: 55.21%(53/96) vs. 23.96%(23/96), 56.25%(54/96) vs. 36.46%(35/96), and there were statistical differences ( P<0.05). According to the Phi coefficient test, there was a positive correlation between NF-κB and β-catenin positivity and condyloma acuminatum ( r>0, P<0.05). After 6 months of follow-up, 35 of the 96 patients relapsed, with a recurrence rate of 36.46% (35/96). The positive rates of NF-κB and β-catenin in the recurrent subgroup were significantly higher than those in the non-recurrent subgroup: 85.71%(30/35) vs. 37.70%(23/61), 85.71%(30/35) vs. 39.34%(24/61), and there were statistical differences ( P<0.05). COX regression analysis showed that the positive expression of NF-κB and β-catenin was related to the recurrence of patients with condyloma acuminatum after treatment ( HR = 4.017, 3.898, 95% CI 1.516 - 10.646, 1.470 - 10.336, P<0.05). The ROC curve showed that NF-κB, β-catenin, and their combination had moderate predictive value for recurrence in patients with condyloma acuminatum after treatment. The area under the curve (AUC) was 0.740 and 0.732, respectively. Among them, the combined predictive value was the highest, with an AUC of 0.810 ( P<0.05). Conclusions:The positive rates of NF-κB and β-catenin in the skin lesions of patients with condyloma acuminatum are relatively high, and they have certain predictive value for the recurrence of patients after treatment.

Objective:To identify risk factors for developing infected pancreatic necrosis (IPN) in female hypertriglyceridemia-induced acute pancreatitis (HTG-AP) patients with childbearing age.Methods:A retrospective analysis was conducted on clinical data from 460 female HTG-AP patients with childbearing age admitted to Eastern Theater General Hospital of the Chinese People's Liberation Army between December 2013 and December 2022. Patients were divided into the IPN group ( n=140) and non-IPN group ( n=320) based on the occurrence of IPN. General clinical data, laboratory test results and clinical outcomes were compared between the two groups. Univariate and multivariate logistic regression models were used to analyze the risk factors for IPN in reproductive-age HTG-AP patients. Results:Among the 460 patients, 140 (30.4%) developed IPN. Compared with the non-IPN group, the IPN group had a significantly higher proportion of pregnant patients (30.0% vs 10.9%, P<0.001) and a lower proportion with a history of acute pancreatitis (12.9% vs 31.3%, P<0.001). Laboratory findings showed that patients in the IPN group had lower levels of hemoglobin and albumin, but higher levels of blood urea nitrogen and serum creatinine. Clinical outcomes showed that the IPN group had significantly higher rates of severe acute pancreatitis and mortality compared to the non-IPN group. Additionally, the median length of ICU stay and total hospital stay in the IPN group were significantly longer, at 16.5 days and 32.0 days, respectively. Univariate logistic regression analyses showed that pregnancy, previous history of acute pancreatitis, and levels of hemoglobin, total bilirubin, albumin, blood urea nitrogen and creatinine were significantly associated with IPN. Multivariate logistic regression analysis revealed that pregnancy ( OR=2.617, 95% CI 1.494-4.210, P=0.001), a history of acute pancreatitis ( OR=0.339, 95% CI 0.189-0.711, P=0.002), hemoglobin level ( OR=0.945, 95% CI 0.939-0.987, P<0.001), and blood urea nitrogen level ( OR=1.173, 95% CI 1.056-1.198, P=0.004) were independently associated with the occurrence of IPN. Conclusions:Pregnancy, a first episode of acute pancreatitis, lower hemoglobin levels and higher blood urea nitrogen levels are independent risk factors for IPN in female HTG-AP patients with childbearing age.

Objective Advances in synthetic DNA technology have made it much easier to fake human DNA samples.There are literature reports that fake human DNA can be synthesized by different methods and implanted in the field to confuse the investigation or mislead the trial.Therefore,distinguishing authentic human DNA from synthetic DNA and performing individual identification has become a critical scientific challenge.Methods We define a novel composite genetic marker(methylation-microhaplotype)by combining CpG sites stably hypermethylated or hypomethylated in natural human DNA and nearby immediately adjacent microhaplotype sites.A total of 19 locis were obtained according to the screening criteria,and a composite detection system for methylation-microhaplotypes was established using MPS technology.Random volunteer DNA samples were extracted and synthetic DNA samples were prepared based on whole genome amplification techniques.Population DNA samples were analyzed to evaluate forensic parameters and methylation variability of the methylation-microhaplotype markers.Comparative analyses of human and synthetic DNA were conducted to assess the markers'ability to discriminate between the two and to detect/type both components in mixed mixed samples.Results The composite detection system composed of 19 locis demonstrated high individual identification ability,achieving a cumulative individual identification probability of 0.999 999 999 996 86.12 hypermethylated locis and 7 hypomethylated locis had relatively stable methylation levels in 57 human DNA samples.According to the allele methylation rate(Ram)value,the system can effectively identify natural and synthetic DNA samples.Meanwhile,for mixed DNA samples,the presence of human and synthetic DNA samples can be found and genotyped.Conclusion Methylation-microhaplotype genetic markers,which can discover human DNA and synthetic DNA and can detect the presence and genotyping of them from mixed samples,is a potential useful tool for forensic DNA analysis.

Psoriasis and atherosclerosis are both complex and refractory.Genetic susceptibility,inflammatory response and the role of adipokines may be the main mechanisms of their comorbidity.TCM believes that heat,toxicity,phlegm,and blood stasis are the basic pathological factors of the comorbidity,which intertwine and lead to a longer course of disease.TCM,starting from the holistic concept,has a certain efficacy in the treatment of psoriasis and atherosclerosis,and has received widespread attention.This article reviewed the pathogenesis of psoriasis and atherosclerosis comorbidity from the aspects of heredity,inflammatory response,and the role of adipokines,and combed the current situation of the research on the mechanism of the treatment of psoriasis and atherosclerosis comorbidity with TCM monomer and compounds,providing references for related mechanisms and clinical research.

Objective:To investigate the therapeutic impact of combining vitamin D supplementation with dietary adjustments on elderly male patients with diabetes and osteosarcopenia.Methods:From January 2021 to May 2023, a total of 108 male patients diagnosed with diabetes and osteosarcopenia were admitted to Beijing hospital for research purposes.The patients were randomly assigned to either a control group( n=54)or a trial group( n=54).The control group received conventional dietary adjustments, while the trial group received vitamin D supplementation in addition to dietary adjustments.Various parameters including 25-(OH)-VitD 3 levels, blood glucose levels, appendicular skeletal muscle mass index(ASMI), bone mineral density, and bone metabolism indexes were measured before and after the treatment in both groups. Results:Compared to the pre-treatment period, the levels of 25-(OH)-VitD 3, grip strength, 6 m step speed, and ASMI increased in both groups, while fasting blood glucose and 2 h postprandial blood glucose decreased significantly(all P<0.05).Additionally, the experimental group showed higher levels of 25-(OH)-VitD 3, grip strength, 6 m step speed, and ASMI compared to the control group post-treatment, with lower levels of fasting blood glucose and 2 h postprandial blood glucose(all P<0.05).Compared with the pre-treatment period (bone density values in the lumbar spine: 0.41±0.09, the hip: 0.42±0.12 in the control group; bone density values in the lumbar spine: 0.43±0.07, the hip: 0.44±0.09 in the experimental group), the bone density values of the lumbar spine and the hip were higher in both groups after treatment(bone density values in the lumbar spine: 0.76±0.12, the hip: 0.78±0.12 in the control group; bone density values in the lumbar spine: 0.95±0.22, the hip: 0.97±0.28 in the experimental group). The bone density values of the lumbar spine and the hip in the experimental group were higher than those of the control group after treatment(all P<0.05).Furthermore, serum concentrations of parathyroid hormone(PTH)and osteoclast differentiation factor(RANKL)were significantly lower, while serum osteocalcin(OCN)was significantly higher in both groups post-treatment(all P<0.05).In the experimental group, serum PTH and RANKL concentrations were significantly lower and serum OCN was significantly higher compared to the control group post-treatment(all P<0.05). Conclusions:The results show that incorporating vitamin D into dietary changes can effectively regulate blood glucose levels, decrease bone loss, enhance bone density, and improve muscle quality in this patient population.

Objective:To explore the diagnostic value of serum C-C chemokine ligand 5 (CCL5) in assessing the degree of liver fibrosis in patients with metabolic-associated fatty liver disease (MAFLD).Methods:71 MAFLD patients who visited the Department of Integrated Traditional and Western Medical Hepatology, Third Hospital of Hebei Medical University, and underwent liver biopsy histopathology examinations between October 2021 and June 2023 were selected for diagnostic testing. Simultaneously, 71 healthy subjects who underwent physical examinations at the physical examination center of the hospital were selected as the control group. Serum CCL5 levels were detected using an enzyme-linked immunosorbent assay (ELISA). Routine blood tests, liver and kidney function tests, and other tests were conducted to analyze the expression level of CCL5 and its correlation with the above indicators. The aspartate aminotransferase/platelet ratio index (APRI) and fibrosis 4 index (FIB-4) were calculated. SPSS 26.0 software was used for statistical analysis. The area under the receiver operating characteristic curve (ROC) was used to evaluate the diagnostic efficacy of CCL5 for the degree of liver fibrosis in MAFLD. The combined diagnostic efficacy of APRI and FIB-4 was further analyzed for the degree of liver fibrosis in MAFLD.Results:The expression level of serum CCL5 gradually increased with the increase in liver fibrosis stage in patients with MAFLD, and the difference was statistically significant ( P<0.05). The AUC value of serum CCL5 for diagnosing significant liver fibrosis in MAFLD patients was 0.775, with a sensitivity of 65.7%, a specificity of 80.6%, and an optimal cutoff value of 49.845 ng/ml. The CCL5 and FIB-4 combination had the highest diagnostic value for significant liver fibrosis in patients with MAFLD, with an AUC of 0.802, a sensitivity of 91.4%, and a specificity of 61.1%. Conclusion:CCL5 has a high diagnostic value for significant liver fibrosis in MAFLD patients. Therefore, it is expected to become a non-invasive diagnostic marker for assessing the degree of liver fibrosis in MAFLD patients.