Objective To explore the effect of polysaccharides from Sargassum graminifolium (SGP) on the intestinal flora of obese mice and the relationship between changes of the intestinal flora and biochemical indicators of blood lipid.Methods Sixty c57BL/6J mice were randomly divided into the conventional feed group (the control group,n =10) and the high-fat feed group (n =50).The model of obesity was established by using the animals in the high-fat feed group.With rejection of 10 animals that were not conformed to the selection standards,the remaining 40 mice were divided into the blank control group and the treatment group.The treatment group was subdivided into the low-dosage group that was given SGP at a dosage of 50 mg/kg by gavage,the medium-dosage group that was given SGP at a dosage of 100 mg/kg by gavage,and the high-dosage group that was given SGP at a dosage of 200 mg/kg by gavage,each consisting of 10 animals.After 8 weeks of feeding the SGP by gavage,detections were performed in biochemical indicators of blood lipid and the number of colonies of Escherichia coli,lactobacillus and bifidobacterium in the contents of the appendix.Results The number of colonies of lactobacillus [(7.05 ± 0.15)lg CFU/g] and that of bifidobacterium [(8.75 ± 0.41)lg CFU/g] in the blank control group was significantly decreased,as compared with that of the control group [(8.31 ± 0.12)lg CFU/g and(10.17 ± 0.31) lg CFU/g].The number of colonies of Escherichia coli in the blank control group [(6.87± 0.25)lg CFU/g] was significantly higher,as compared with that of the control group [(5.83 ± 0.28) lg CFU/g],and statistical significance could be seen,when comparisons were made between them(P ＜ 0.05).When compared with that of the blank control group,the number of colonies of Escherichia coli in the medium-dosage and high-dosage groups were significantly decreased.The number of colonies of lactobacillus and bifidobacterium in the low-dosage,medium-dosage and high-dosage groups were significantly increased.The number of colonies of bifidobacterium in the low-dosage and high-dosage groups were also significantly increased,with statistical significance(P ＜0.05).TC [(5.18 ± 0.87) mmol/L],TG [(1.90 ± 0.21) mmol/L] and LDL [(0.63 ± 0.02) mmol/L] were all higher than those of the control group,but were lower than those in the blank group,which were respectively [TC (2.54 ± 0.39) mmol/L],[TG (1.12 ± 0.20) mmol/L] and [LDL(0.35 ±0.05)mmol/L].As compared with those of the blank control group,the levels of HDL in the low-dosage,medium-dosage and high-dosage groups were all decreased significantly.The levels of TG in the medium-dosage and high-dosage groups were also decreased significantly,the levels of LDL in the low-dosage,medium-dosage and high-dosage groups were all decreased significantly,and the levels of HDL in the mediumdosage and high-dosage groups were all increased significantly.Statistical significance could be noticed,when comparisons were made between them(P ＜ 0.05).The levels of Escherichia coli were positively correlated with those of TC,TG and LDL,while the levels of HDL coli were negatively correlated with those of TC,TG and LDL.The levels of lactobacillus and bifidobacterium were negatively correlated with those of TC,TG and LDL,however,they were positively correlated with those of HDL.Conclusions SGP could suppress the growth of Escherichia coli in the intestinal of the high-fat-feed mice,and the number of intestinal colonies was correlated with the biochemical indicators of blood lipid.

Objective To explore the effect of polysaccharides from Sargassum graminifolium (SGP) on the intestinal flora of obese mice and the relationship between changes of the intestinal flora and biochemical indicators of blood lipid.Methods Sixty c57BL/6J mice were randomly divided into the conventional feed group (the control group,n =10) and the high-fat feed group (n =50).The model of obesity was established by using the animals in the high-fat feed group.With rejection of 10 animals that were not conformed to the selection standards,the remaining 40 mice were divided into the blank control group and the treatment group.The treatment group was subdivided into the low-dosage group that was given SGP at a dosage of 50 mg/kg by gavage,the medium-dosage group that was given SGP at a dosage of 100 mg/kg by gavage,and the high-dosage group that was given SGP at a dosage of 200 mg/kg by gavage,each consisting of 10 animals.After 8 weeks of feeding the SGP by gavage,detections were performed in biochemical indicators of blood lipid and the number of colonies of Escherichia coli,lactobacillus and bifidobacterium in the contents of the appendix.Results The number of colonies of lactobacillus [(7.05 ± 0.15)lg CFU/g] and that of bifidobacterium [(8.75 ± 0.41)lg CFU/g] in the blank control group was significantly decreased,as compared with that of the control group [(8.31 ± 0.12)lg CFU/g and(10.17 ± 0.31) lg CFU/g].The number of colonies of Escherichia coli in the blank control group [(6.87± 0.25)lg CFU/g] was significantly higher,as compared with that of the control group [(5.83 ± 0.28) lg CFU/g],and statistical significance could be seen,when comparisons were made between them(P ＜ 0.05).When compared with that of the blank control group,the number of colonies of Escherichia coli in the medium-dosage and high-dosage groups were significantly decreased.The number of colonies of lactobacillus and bifidobacterium in the low-dosage,medium-dosage and high-dosage groups were significantly increased.The number of colonies of bifidobacterium in the low-dosage and high-dosage groups were also significantly increased,with statistical significance(P ＜0.05).TC [(5.18 ± 0.87) mmol/L],TG [(1.90 ± 0.21) mmol/L] and LDL [(0.63 ± 0.02) mmol/L] were all higher than those of the control group,but were lower than those in the blank group,which were respectively [TC (2.54 ± 0.39) mmol/L],[TG (1.12 ± 0.20) mmol/L] and [LDL(0.35 ±0.05)mmol/L].As compared with those of the blank control group,the levels of HDL in the low-dosage,medium-dosage and high-dosage groups were all decreased significantly.The levels of TG in the medium-dosage and high-dosage groups were also decreased significantly,the levels of LDL in the low-dosage,medium-dosage and high-dosage groups were all decreased significantly,and the levels of HDL in the mediumdosage and high-dosage groups were all increased significantly.Statistical significance could be noticed,when comparisons were made between them(P ＜ 0.05).The levels of Escherichia coli were positively correlated with those of TC,TG and LDL,while the levels of HDL coli were negatively correlated with those of TC,TG and LDL.The levels of lactobacillus and bifidobacterium were negatively correlated with those of TC,TG and LDL,however,they were positively correlated with those of HDL.Conclusions SGP could suppress the growth of Escherichia coli in the intestinal of the high-fat-feed mice,and the number of intestinal colonies was correlated with the biochemical indicators of blood lipid.

Objective To introduce the clinical types of perforator branches of anterosuperior malleolus flap and explore its application.Methods Anterosuperior malleolus flap coupling with dorsal pedal flap was used for repairing the soft tissue defect of hands in 18 patients,in which anterosuperior malleolus flap-dorsal pedal single flap in 12 cases,anterosuperior malleolus flap-dorsal pedal bilobate flap in 4 cases,anterosuperior malleolus flap-dorsal pedal trilobate flap in 2 cases;Anterosuperior malleolus retrograde island (bone) flap was used in recovering pedal soft tissue in 22 patients,the flap pedicled from stem of anterior tibial artery in 16 cases,dorsal pedal flap-anterosuperior malleolus flap in 2 cases,the flap from perforate vessels without injuring the anterior main tibial artery in 2 cases,the bone flap combined with the distal of tibia in 2 cases.Results In the 18 cases of hands,17 cases survived,and 1 case of flap mild necrosis at the distal site took a second-phase skin-grafting to repair.Twenty cases of anterosuperior malleolus retrograde island (bone) flap survived,and the other 2 cases needed secondary skin-grafting to repair the necrosis edge of flaps because of venous limited.After a follow-up from 3 to 6 months,30 cases showed the satisfied postoperative outlook,with the good healing of the donor sites.Typing the 40 cases according to the location of perforator branches of the anterosuperior malleolus flap,20 cases locate in the medial of anterior tibial muscle,16 cases locate between the anterior tibial muscle and extensor hallucis longus,4 cases locate between extensor hallucis longus and extensor digitorum longus.Conclusion Knowing the clinical types of the perforator branches of anterosuperior malleolus flap is not only helpful for the accurate processes of operations、preventing cutaneous branches,but also improving the success rate of surgery.