[Objective]To construct the new training mode of professional master degree of Chinese material medica. [Method]Based on idea of work and practice in Guangzhou University of Traditional Chinese Medicine, we put forward the training mode of professional master degree of Chinese material medica. [Result]In the course of training of three years, insistency should be persisted in object, claim, check and management. To choose a graduate teacher respectively in the col ege and the enterprise, they have their own emphasis on training. Look upon the continuous training, elaborately select the practical case, and first study in col ege, and then practice in enterprise. [Conclusion]The training mode is putting forwardIt is the same and consistent in three years, the system is of double teachers, al are for use, and production is continuously bound up with learning”,worth other TCM col eges learning.

OBJECTIVETo study the compatibility of dosage change of Zhishi, Houpo and Mangxiao affecting the yields of Anthraquinone components in Dachengqi decoction.

METHODResponse surface methodology (RSM) with Doehlert design was adopted to evaluate the yields of Anthraquinone components in Dachengqi decoction by dosage change of Zhishi, Houpo and Mangxiao and the analysis time was shorten through a desirability function.

RESULTResults show that Anthraquinone components were got a high yields when the dosage ratio of Dachengqi decoction were compatible as follows: Dahuang-Zhishi-Houpo-Mangxiao (1:4:2. 31:2).

CONCLUSIONThe Doehlert design with a desirability function, which allow a sequential response methodology, is a good methods, and, of cause, the mathematical model can be further extended and applied to the compatibility research of Chinese material medicine.

Anthraquinones ; analysis ; Dosage Forms ; Drugs, Chinese Herbal ; administration & dosage ; chemistry ; Models, Chemical ; Plant Extracts ; administration & dosage ; chemistry

AIM　To investigate the relationship between sinomenine distribution and its organic toxicology in rats so as to give some pharmacological data for clinical application of sinomenine. METHODS　Three kinds of administration plans were designed in the experiment, ie sinomenine was ip administered at the dosage of l50 mg*kg-1 per day, repreat-dosed for 6 wk and suspended the drug for 1 wk after 6wk repeat-doses．At the end of the each administration plan，the animals were sacrificed and their blood and their main internal organs were collected for the purpose of measurement of sinomenine concentration in each sample by HPLC. Meanwhile，the histopathological and serological examinations were also done in the experiments. RESULTS　The sinomenine concentration in rats internal organs were in order of liver, heart, lung and brain either in single-dosed treated animals or in repeat-dosed treated animals for 6 wk. However，the concentration of sinomenine could not be detected by HPLC after l wk drug-suspension，the histopathological examination showed that sinomenine at the dosage of l50 mg*kg-l per day for 6wk treatment could slightly damage liver ce11s, dominant1y caused the cell edema，but no any influence on the sero1ogy of liver and kidney. Sinomenine ip could also cause a mild hyperaemia of the rats heart tissues but no any histopathological changes had been observed. In testis tissues no sinomenine had beed detected although the animals were treated by repeat treatment for 6 wk and no any histopathological changes had been found yet. However, Sinomenine could partialy inhibit the sperm vitalities and amount of the dead sperms were a1so augmented. It was similar to in vitro eperiments. These influences of sinomenine on testis could be quickly recovered by drug suspension. CONCLUSION　Sinomenine concentration were in order of liver, heart, kidney, lung and brain either in treatment by single dose or by repeat-dose administration. The histopathological changes were only abserved in liver cells of the animals which indicates that it should be in consideration of the liver functions during treatment course of the drug.

The identification of Radix Morindae Officinalis, Radix Rehmanniae Preparata, Herba Epimedii, Radix Ginseng, Radix Achyranthis Bidentatae, Rhizoma Dioscoreae in Baji Bushen decoction was carried out respectively by TLC. The content of icariin in the preparation was determined by HPLC. Chromatocolumn was adopted, methanol - 0. 5 % HClO4 (55: 45) as the mobile phase, flow - rate at 0.8 mL/min, column temperature at 40℃, detection wavelength at 270nm, the mean recovery is 97. 36%and RSD is 2.61% (N=5). It is indicated that the methods can be used for the quality control of Baji Bushen decoction.

Objective To study the content difference of naringin in Fructus Aurantii Immaturus of different specifications.Methods HPLC was performed on the Merck- Lichrospher RP-C18(4.6 mm? 250 mm, 5 ? m) with temperature of 35 ℃ .The chromatographic conditions for naringin:the mobile phase consisting of acetonitrile-0.3 % Phosphoric acid(20 ∶ 80), at the flow rate of 1 mL/min, and the detection wavelength at 283 nm. Results The content of naringin increased with the diameter of Fructus Aurantii Immature slices.Conclusion It is suggested that there exists significant differences in intrinsic quality among Fructus Aurantii Immaturus of different specifications.

Objective To establish an ICP-MS method for the determination of heavy metals,including copper(Cu),arsenium(As),cadmium(Cd) and plumbum(Pb),in five kinds of Chinese medicinal material.Methods The samples were digested by closed-vessel microwave.The four heavy metals were directly analyzed by ICP-MS,with elements 72Ge,115In,and 209Bi as the internal standards.Results For all of the analyzed heavy metals,the correlative coefficient of the calibration curves was in the range of 0.999 5～ 0.999 7.The recovery rates were in the range of 92.3 % ～ 108.0 %,and RSD were in the range of 2.4 % ～ 5.8 %.Conclusion The method is convenient,rapid,accurate and can be applied to determine four heavy metals,including Cu,As,Cd and Pb,in Chinese medicinal material.

Objective To evaluate the refinement process for extracting Herba Artemisiae Scopariae(HAS) with macroporous resin.Methods With reservation rate of main peak in the fingerprint as the index,the refinement process for extract of HAS was evaluated.The safety of oral administration of HAS extract before and after refinement process was evaluated by detecting serum levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST),observing the hepatic histopathological changes in mice model of CCl4-induced acute hepatic injury,and by mice acute toxicity experiment.Results The fingerprint of HAS extract before and after treatment was similar,the difference of their hepatoprotective effect was insignificant,and there was no obvious acute toxic reaction of HAS extract before and after refinement.Conclusion This experiment verified the rationality of the refinement process for extracting Herba Artemisiae Scopariae with macroporous resin by the method of pharmacodynamics combining with HPLC fingerprint.

AIM: To study the influencing factors of static adsorpting process of extract of Herba Artemisiae scopariae with macroporous resin,and determine the refinement process. METHODS: The absorbances and the adsorption quantity of caffeotannic acid were used as indexes,the adsorption effect of 9 kinds of macroporous resin and static adsorption curve of F resin;density and pH of the extract liquor were investigated by use of factorial experiment;HPLC fingerprint of extract of Herba Artemisiae scopariae were evaluated the adsorption effect.(RESULTS:) The adsorpting effect were different among types of macroporous resin,and the adsorpting equilibrium time was 6 h in the use of F resin;Density and pH of the extract liquor are important factors of adsorption;The experiment indicated that decreased liquor by this process were important factors of adsorption;The experiment indicated that this process decreased the yield of extract by 2.5%. CONCLUSION: The result can offer information about the determination of refinement process of extract of Herba Artemisiae scopariae with macroporous resin.

AIM: To study the kinetic adsorption process in the use of the extraction of Herba Artemisiae scopariae. METHODS: In combination with caffeotannic acid and the absorbance, HPLC fingerprint of extract of Herba Artemisae scopariae was adopted as marker, to investigate the kinesis adsorb and elution process, the to determine the max quantity of physic liquor and the type and quantity of elution solvent. RESULTS: The max adsorption quantity of caffeotannic acid that could adsorb was 20.9 mg/g dry resin, and the elution solvent determined was five-fold column volume of 80% alcohol; The repeated experiment indicated that this process decressed to 2.5% yield of extract and meanwhile it retained the whole components of the HPLC fingerprint effectly. CONCLUSION: The kinetic adsorption can enrich the active components in Herba Artemisiae scopariae effectively.

Objective: To establish a HPLC method for the determination of synephrine in Dachengqi Granules (Radix et Rhizoma Rhei, Fructus Aurantii Immaturus, Cortex Magnoliae Officinalis, Natrii Sulfas). Methods: A E. Merck LiChrospher 100RP 18 column was /used. The mobile phase was methanol water (contained 0.18% H 3PO 4 and 0.22% C 12 H 25 SO 4Na) (55∶45). The detection wavelength was at 274nm.Results: The linear range of synephrine was 0.14?g～1.54?g, r =0.9998. The average recovery was 98.1%, RSD =2.1% ( n =5).Conclusion: The result is accurate and the reproducibility is good. This method can be used for the determination of synephrine in Dachengqi Granules.