Objective To evaluate the regulatory effect of the adaptor related protein complex 2 subunit μ1(AP2M1)on proliferation and invasion of diffuse large B-cell lymphoma(DLBCL).Methods Human diffuse large B-cell lymphoma cell line OCI-LY8 was aliquoted into control group,NC-shRNA group,AP2M1-shRNA group,NC-LV group,and AP2M1-LV group.Lipofectamine 2000 was used for cell transfection.Cell proliferation was detected by tetramethylazolium salt(MTT)method,apoptosis was detected by flow cytometry and cell migration and invasion were detected by Transwell assay.The protein expression of AP2M1,epidermal growth factor receptor(EGFR),p-phosphatidylinositol 3 kinase(PI3K),PI3K,p-protein kinase B(Akt)and AKT was detec-ted by Western blot.Results Compared with control group,the relative expression of AP2M1 mRNA and protein in the AP2M1-shRNA group was decreased(P＜0.05).The relative cell viability was increased(P＜0.05).The cell apoptosis rate was decreased(P＜0.05).The counting number of migrating and invading cells was in-creased(P＜0.05).The relative expression level of EGFR protein and the phosphorylation level of PI3K and AKT were increased(P＜0.05).Compared with Control group,the expression of AP2M1 mRNA and protein relative ex-pression level in AP2M1-LV group was increased(P＜0.05).The relative cell viability was decreased(P＜0.05).The cell apoptosis rate was increased(P＜0.05).The number of migrating and invading cells was decreased(P＜0.05).The relative expression level of EGFR protein and the phosphorylation level of PI3K and AKT were all decreased(P＜0.05).Conclusions The over-expression of AP2M1 partially inhibits the proliferation and invasion of DLBCL cells by inhibiting the EGFR/PI3K/AKT signaling pathway.

Objective: To observe the clinical efficacy of combined acupuncture and speech rehabilitation in treating postapoplectic aphasia. Methods: Sixty subjects were randomized into two groups. 30 cases in the treatment group were treated by both speech rehabilitation and acupuncture therapy, while 30 cases in the control group were treated by speech rehabilitation only. After 30-day treatments, the clinical efficacy was observed. Results: The total effective rate and speech score were higher in the treatment group than in the control group (P<0.05). Conclusion: Combined acupuncture and speech rehabilitation is quite effective for postapoplectic aphasia.

Objective: To observe the clinical efficacy of acupuncture therapy for postherpetic neuralgia in the elders. Methods: Twenty-four cases of postherpetic neuralgia were randomized into two groups, an acupuncture group in which 12 cases were treated by acupuncture at Jiaji (Ex-B 2) on the diseased side, and a medication group in which 12 cases were treated by oral obtundent and intramuscular injection of neurotrophic drugs. Results: After four-week treatments, the total effective rate was 91.6% in the acupuncture group and 83.3% in the medication group. Conclusion: Acupuncture can significantly relieve postherpetic neuralgia, with better effects than medication.

Objective:To observe the clinical efficacy of acupuncture and cupping on the back in the treatment of allergic rhinitis.Methods:Thirty-one cases of allergic rhinitis were treated by acupuncture and moving cupping,twice a week;five treatments made up one course and two-course treatments were given.Result:Sixteen cases were cured,14 cases improved and 1 case failed.Conclusion:Acupuncture plus cupping has positive effects on allergic rhinitis.

Objective:To observe the clinical efficacy of warm acupuncture in the treatment of diabetic peripheral neuropathy and its effects on the conduction velocity of the tibial nerve and common fibular nerve.Methods:Fifty.two subjects were evenly randomized into two groups and treated by conventional therapy;in addition,the control group was given mecobalamin injection at 500 μg,once a day for four weeks,and treatment group was given warm acupuncture,6 times a week for 4 weeks.The clinical efficacy and the conduction velocity of the tibial nerve and common fibular nerve were observed and compared between the two groups.Results:After four-week treatments.the total effective rate Was 88.5% in the treatment group and 61.5% in the control group,with a higher rate in the treatment group than in the control group (P＜0.05):the conduction velocity of tibial nerve and common fibular nerve Was higher in both groups(P＜0.05),with higher conduction velocity in the treatment group than in the control group (P＜0.05).Conclusion:Warm acupuncture is quite effective to treat diabetic peripheral neuropathy and to improve nerve velocity.

Objective: To observe the clinical efficacy of needling nape acupoints in the treatment of pseudobulbar palsy. Methods: One hundred and twenty cases of pseudobulbar palsy were randomized into two groups, a treatment group in which 60 cases were treated by needling nape acupoints and a control group in which 60 cases were treated by needling tongue acupoints; after one-month treatment, the clinical symptoms and signs were observed. Results:The cure rate and total effective rate were respectively 41.7% and 90.0% in the treatment group,and respectively 26.7% and 81.7% in the control group, with differences in the cure rate and total effective rate between the two groups (P＜0.01). Conclusion: Needling nape acupoints is quite effective to treat pseudobulbar palsy in the relief of clinical symptoms and signs.

Objective:To observe the clinical efficacy of nerve-near needling for occipital neuralgia.Methods:Eighty-two subjects were randomly allocated into two groups:treatment group in which 46 cases was treated by nerve-near needling[points Tianzhu(BL 10)and Tianyou(TE 16)were selected],and control group in which 36 cases were treated by conventional acupuncture[points Tianzhu(BL 10),Fengchi(GB 20),Baihui(GV 20),Shimian Ⅱ (Extra),Wangu(GB 12)and Hegu(LI 4)were selected].One course of treatment consisted of 3.5 times,and three courses of treatment were given at most before calculating efficacy.Results:The short-term total effective rate was 93.5%in the treatment group and 69.4%in the control group,with a statistically significant difference between the two groups(P＜0.01);the long-term total effective rate Was 64.1%in the treatment group and 43.3%in the control group,with a statistically significant difierence between the two groups(P＜0.05).Conclusion:Nerve-near needling is better than conventional acupuncture in treating occipital neuralgia.

Objective:To observe the clinical efficacy of tuina therapy in the treatment of cervical spondyIosis of vertebral artery type.Methods:One hundred and fifty-seven cases of cervical spondylosis of vertebral artery type were treated by tuina therapy,three times a week.Ten treatments made up one course and one to two courses of treatment Was performed.During tuina treatment,other modalities that may affect tuina effects were discontinued.The patients were surveyed three months after tuina treatment.Results:Among the 157 cases,109 cases obtained marked effectiveness,26 cases improvement and 6 cases inefiectiveness;16 cases were not surveyed.The total effective rate was 86.6%.Conclusion:Tuina therapy has excellent effects on cervical spondylosis of vertebral artery type.

Objective:To investigate the relationship between fester capacity and clinical efficacy on allergic asthma treated by scarring moxibustion.Methods:The patients were randomly divided into scarring moxibustion 9-cone group,3-cone group and 6-cone group.The biggest area,scab-lost time,first-festering time and scarring time of moxibustion sore,and the clinical efficacy in each group was observed.Results:The biggest post-moxibustion sore area in 9-cone group was larger than that in 3-cone group,but had no difference in comparison with 6-cone group;the scab-lost time,first-festering time and scarring time of post-moxibustion sore in each group were of no difference.The clinical efficacy was better in 9-cone group than in 3-cone group,but had no difference in comparison with the 6-cone group.Conclusion:Fester capacity in treating allergic asthma by scarring moxibustion is related to the clinical efficacy and definite fester capacity is the key to good results on allergic asthma.

Objective: To observe the clinical efficacy of combined acupuncture and herbs in the treatment of chronic pelvic inflammation. Methods: Eighty-eight cases of chronic pelvic inflammation were randomized into two groups. Treatment group (45 cases) was treated by combined acupuncture and herbs, control group (43 cases) was treated by Fu Ke Qian Jin Jiao Nang (Female Herbal Capsule). Results: After 3 menstrual cycles of treatments, in treatment group, 28 cases were cured, 9 cases got marked effect, 4 cases got effect, 4 cases were failure, and the total effective rate was 91.1%; in control group, 7 cases were cured, 8 cases got marked effectiveness, 15 cases got effectiveness, 13 cases were failure, and the total effective rate was 69.8%. The total effective rate was higher in treatment group than in control group (P<0.05); in the six-month survey, the relapse rate was lower in treatment group than in control group (P<0.01). Conclusion: Combined acupuncture and herbs is effective for chronic pelvic inflammation, with fewer relapses.