In order to study whether there is a direct interaction between the key variant gene(AP-PV-YN-Mut)protein of atypical porcine pestivirus(APPV)Yunnan strain and the host protein signal sequence receptor subunit 4(SSR4),the physical and chemical properties,spatial structure and subcellular localization of SSR4 protein were analyzed and predicted using online software such as ProtParam,PredictProtein and TMHMM.The recombinant vectors pCMV-Tag4A-SSR4 and pET-GST-Mut were constructed for GST pull-down test in vitro.The recombinant vectors pBiFC-VN173-SSR4 and pBiFC-VC155-Mut were constructed and the bimolecular fluorescence comple-mentary test(BiFC)was performed in cells to verify whether there was direct interaction between APPV-YN-Mut and host protein SSR4 in vitro and in cells.The results showed that SSR4 protein was a hydrophobic stable protein with no transmembrane structure and signal peptide.The second-ary structure was mainly irregular curl,and the tertiary structure was stable,mainly located in the endoplasmic reticulum.GST pull-down and BiFC experiments showed that APPV-YN-Mut interac-ted directly with host protein SSR4 in vitro and in cells.

In order to study whether there is a direct interaction between the key variant gene(AP-PV-YN-Mut)protein of atypical porcine pestivirus(APPV)Yunnan strain and the host protein signal sequence receptor subunit 4(SSR4),the physical and chemical properties,spatial structure and subcellular localization of SSR4 protein were analyzed and predicted using online software such as ProtParam,PredictProtein and TMHMM.The recombinant vectors pCMV-Tag4A-SSR4 and pET-GST-Mut were constructed for GST pull-down test in vitro.The recombinant vectors pBiFC-VN173-SSR4 and pBiFC-VC155-Mut were constructed and the bimolecular fluorescence comple-mentary test(BiFC)was performed in cells to verify whether there was direct interaction between APPV-YN-Mut and host protein SSR4 in vitro and in cells.The results showed that SSR4 protein was a hydrophobic stable protein with no transmembrane structure and signal peptide.The second-ary structure was mainly irregular curl,and the tertiary structure was stable,mainly located in the endoplasmic reticulum.GST pull-down and BiFC experiments showed that APPV-YN-Mut interac-ted directly with host protein SSR4 in vitro and in cells.

Objective To study the characteristics of the head shape and correlations among the shape parameters in infants at the plagiocephaly.Methods One hundred and four infants at the age of 4-12 months from Department of Rehabilitation,Guangzhou Women and Children's Medical Center with plagiocephaly or normal head shape from January to October 2016 were selected for head shape characteristic analysis.Seventy-four males and 30 females were included.Seventy-three infants (53 males and 20 females) with plagiocephaly were plagiocephaly group with age of (7.07 ±2.23) months.Thirty-one infants (21 males and 10 females) with normal head shape were control group with age for (8.29 ± 2.81) months.The Spectra scanner 2.0 was applied to acquire head shape parameters including cephalic ratio (CR),radial symmetry index (RSI),oblique diagonal difference (ODD),and cranial vault asymmetry index (CVAI).The correlation between the head shape characteristics and shape parameters of infants in different month ages and gender with plagiocephaly were analyzed through independent sample t-test and Pearson correlation analysis.Among all the enrolled infants,there were 46 infants in 4-7 months (36 cases with plagiocephaly and 10 cases with normal head shape),34 infants in ＞ 7-10 months (24 cases with plagiocephaly and 10 cases with normal head shape),and 24 infants in ＞ 10-12 months (13 cases with plagiocephaly and 11 cases with normal head shape).Results CR in 4-7 months group and ＞ 7-10 months of plagiocephaly group were all significantly higher than that in control group [(91.32 ± 5.60) ％ vs.(80.73 ± 2.68) ％,(90.31 ± 6.22) ％ vs.(84.14 ± 3.57) ％],and the differences were all statistically significant (t =-5.765,-2.924,all P ＜ 0.05).The difference was not significant of the CR in ＞ 10-12 months plagiocephaly group compared with that in control group (t =-1.917,P =0.060).RSI,ODD,and CVAI of plagiocephaly group in all ages were significantly higher than those in control group [(46.40 ± 13.59) mmvs.(16.13 ±4.18) mm,(11.84±3.58) mmvs.(3.44±1.59) mm,7.92±2.43 vs.2.34± 1.07],and the differences were all statistically significant (t =-17.210,-16.579,-16.304,all P ＜ 0.001).CR,RSI,ODD,and CVAI of males and females in plagiocephaly group were respectively higher than those of control group,and the differences were all statistically significant (all P ＜ 0.001).There was no statistical significance in CR,RSI,ODD,CVAI between males and females in plagiocephaly group or control group (all P ＞ 0.05).Pearson correlation analysis revealed that there was no correlation between CR and RSI,ODD,CVAI in any age of plagiocephaly group (all P ＞ 0.05).There was a positive correlation among RSI,ODD,and CVAI in the plagiocephaly group in any age (r =0.940,0.912,0.973,all P ＜ 0.001).Conclusions In the infants with plagiocephaly may exist asymmetric head shape and the shorter length of head at the same time.When one of the values of RSI,ODD and CVAI increases,the other two values increase too with more serious level of plagiocephaly.

OBJECTIVE:To prepare oceanic lysozyme film and establish a method for its quality control.METHODS:Film agent was prepared with oceanic lysozyme as the principle agent and hydroxy propyl methylcellulose as the film-former.Folin-phenol method and agar plate diffusion method were used respectively to determine the concentration of albumen and enzyme activity of oceanic lysozyme;and the content of lysozyme in the oceanic lysozyme film was determined by ultraviolet spectrophotometry.RESULTS:Both albumen concentration and enzyme activity of oceanic lysozyme were up to the bacte?riostatic requirement.The average recovery rate of sample was101.0%(RSD=0.67%).Storing within6months,each index of the sample experienced no marked change.CONCLUSION:The preparation is simple in production,controllable in quality and it served as a new preparation for the clinical treatment of vaginitis.