Objective To analyze current situation and frontier trends of sarcopenic dysphasia in the last decade.Methods Researches about sarcopenic dysphasia were retrieved in the Web of Science Core Collection,from January,2015 to December,2024,and analyzed with Citespace 6.2.R3,in terms of countries,authors,institutions and key-words.Results A total of 1 071 literatures in English were included,showing an upward trend in publication volume.Japan ranked the highest in publication countries,and Institute of Science Tokyo contributed the most.Wakabayashi,Hidetaka published the most articles.The top three high-frequency keywords were deglutition disorders,tongue pressure and inclusion body myositis.The most bursting keywords included tongue pressure,neuromuscular dis-ease and muscular dystrophy,while muscle strength,oral hypofunction,idiopathic inflammatory myopathy and esophageal cancer were the new bursting keywords.Conclusion The researches in the field of sarcopenic dysphagia are increasing in recent years,focusing on the assess-ment,diagnosis and treatment.Future researches may involve in tongue pressure,skeletal muscle mass and oral frailty.

Anaplastic lymphoma kinase (ALK) rearranged renal cell carcinoma (ALK-RCC) is an exceedingly rare malignancy, recently classified as a distinct molecular entity in the 5th edition of the WHO classification for urinary and male genital tumors. Due to its non-specific clinical symptoms and diverse histopathological patterns, accurate diagnosis is difficult. This paper reports a case of ALK-RCC with morphology and immunophenotype resembling papillary renal cell carcinoma. After second-generation sequencing, EML4-ALK gene fusion was found, and positive staining for ALK was confirmed by immunohistochemistry subsequently. Following informed consent from the patient, targeted therapy with crizotinib was initiated. During a 17-month follow-up period, no recurrence or metastasis was observed.

Objective To analyze current situation and frontier trends of sarcopenic dysphasia in the last decade.Methods Researches about sarcopenic dysphasia were retrieved in the Web of Science Core Collection,from January,2015 to December,2024,and analyzed with Citespace 6.2.R3,in terms of countries,authors,institutions and key-words.Results A total of 1 071 literatures in English were included,showing an upward trend in publication volume.Japan ranked the highest in publication countries,and Institute of Science Tokyo contributed the most.Wakabayashi,Hidetaka published the most articles.The top three high-frequency keywords were deglutition disorders,tongue pressure and inclusion body myositis.The most bursting keywords included tongue pressure,neuromuscular dis-ease and muscular dystrophy,while muscle strength,oral hypofunction,idiopathic inflammatory myopathy and esophageal cancer were the new bursting keywords.Conclusion The researches in the field of sarcopenic dysphagia are increasing in recent years,focusing on the assess-ment,diagnosis and treatment.Future researches may involve in tongue pressure,skeletal muscle mass and oral frailty.

Anaplastic lymphoma kinase (ALK) rearranged renal cell carcinoma (ALK-RCC) is an exceedingly rare malignancy, recently classified as a distinct molecular entity in the 5th edition of the WHO classification for urinary and male genital tumors. Due to its non-specific clinical symptoms and diverse histopathological patterns, accurate diagnosis is difficult. This paper reports a case of ALK-RCC with morphology and immunophenotype resembling papillary renal cell carcinoma. After second-generation sequencing, EML4-ALK gene fusion was found, and positive staining for ALK was confirmed by immunohistochemistry subsequently. Following informed consent from the patient, targeted therapy with crizotinib was initiated. During a 17-month follow-up period, no recurrence or metastasis was observed.

Objective This study aimed to analyze the use of various virtual surgical planning and 3D printing templates in mandibular angle ostectomy.Methods Twelve patients with mandibular angle prominence were selected from the Affiliated Stomatological Hospital of Guizhou Medical University between January 2022 and January 2023.Preoperative craniofacial CT scans were used to create three-dimensional models.Two types of virtual surgical planning and templates were designed:ascend-ing branch-fixed templates on the ramus(Group Ⅰ)and inferior border-fixed templates on the excised mandibular angle(GroupⅡ).Patients were divided into two groups based on the template used during surgery.The changes in the mandibular angle be-fore and two weeks after surgery were compared between the groups,and deviations between the surgical plans and postopera-tive results were measured.Results All patients healed successfully with satisfactory cosmetic outcomes.Lateral mandibular angles were observed in all patients of Group Ⅰ.In Group Ⅱ,one patient presented with a lateral angle,while the others showed medial angles.An increase in the mandibular angle(P＜0.01)was noted with no significant difference between the bilateral an-gles(P＞0.05).The deviation from the surgical planning was also not significantly different between the groups(P＞0.05).Conclusion Virtual surgical planning and 3D printed templates in mandibular angle ostectomy result in high accuracy and favor-able cosmetic outcomes.Ramus-fixed template is suitable for lateral mandibular angle type,while lower edge-fixed template is better for medial mandibular angle type.

Background: Immunochemotherapy has demonstrated a promising efficacy for a variety of B-cell lymphoma but has limited efficacy for Epstein–Barr virus-positive (EBV +) diffuse large B-cell lymphoma (DLBCL) that is refractory or relapsed to conventional chemotherapy regimens. Considering higher programmed death-ligand 1 (PD-L1) expres‑ sion in the subset of patients with DLBCL with positive EBV, we speculated that PD-1 inhibitors plus chemotherapy may be an alternative regimen in patients with refractory/relapsed EBV + DLBCL. Methods: This retrospective study included six adult patients diagnosed with refractory EBV + DLBCL resistant to first-line immunochemotherapy regimens (R-CHOP). These patients received PD-1 inhibitors plus chemotherapy as second-line treatment. Results: The final analysis included six patients (four men and two women (median age, 50 years; range, 39–83 years)). Four patients were diagnosed with Epstein–Barr virus (EBV) + DLBCL, and two had DLBCL associated with chronic inflammation. Over a median follow-up of 20 months (range, 2–31 months), the objective response rate was 83% (5/6) and the complete remission rate was 67% (4/6). No severe immune-related adverse reactions occurred, and only a mild rash was reported, which did not necessitate the discontinuation of therapy. Conclusion The combination of PD-1 inhibitors and chemotherapy offers promising results as a second-line treat‑ ment for patients with refractory EBV + DLBCL that is resistant to first-line immunochemotherapy regimens. These preliminary findings warrant further investigation in larger clinical trials to validate the efficacy and safety of this therapeutic approach.

Background: Immunochemotherapy has demonstrated a promising efficacy for a variety of B-cell lymphoma but has limited efficacy for Epstein–Barr virus-positive (EBV +) diffuse large B-cell lymphoma (DLBCL) that is refractory or relapsed to conventional chemotherapy regimens. Considering higher programmed death-ligand 1 (PD-L1) expres‑ sion in the subset of patients with DLBCL with positive EBV, we speculated that PD-1 inhibitors plus chemotherapy may be an alternative regimen in patients with refractory/relapsed EBV + DLBCL. Methods: This retrospective study included six adult patients diagnosed with refractory EBV + DLBCL resistant to first-line immunochemotherapy regimens (R-CHOP). These patients received PD-1 inhibitors plus chemotherapy as second-line treatment. Results: The final analysis included six patients (four men and two women (median age, 50 years; range, 39–83 years)). Four patients were diagnosed with Epstein–Barr virus (EBV) + DLBCL, and two had DLBCL associated with chronic inflammation. Over a median follow-up of 20 months (range, 2–31 months), the objective response rate was 83% (5/6) and the complete remission rate was 67% (4/6). No severe immune-related adverse reactions occurred, and only a mild rash was reported, which did not necessitate the discontinuation of therapy. Conclusion The combination of PD-1 inhibitors and chemotherapy offers promising results as a second-line treat‑ ment for patients with refractory EBV + DLBCL that is resistant to first-line immunochemotherapy regimens. These preliminary findings warrant further investigation in larger clinical trials to validate the efficacy and safety of this therapeutic approach.

Background: Immunochemotherapy has demonstrated a promising efficacy for a variety of B-cell lymphoma but has limited efficacy for Epstein–Barr virus-positive (EBV +) diffuse large B-cell lymphoma (DLBCL) that is refractory or relapsed to conventional chemotherapy regimens. Considering higher programmed death-ligand 1 (PD-L1) expres‑ sion in the subset of patients with DLBCL with positive EBV, we speculated that PD-1 inhibitors plus chemotherapy may be an alternative regimen in patients with refractory/relapsed EBV + DLBCL. Methods: This retrospective study included six adult patients diagnosed with refractory EBV + DLBCL resistant to first-line immunochemotherapy regimens (R-CHOP). These patients received PD-1 inhibitors plus chemotherapy as second-line treatment. Results: The final analysis included six patients (four men and two women (median age, 50 years; range, 39–83 years)). Four patients were diagnosed with Epstein–Barr virus (EBV) + DLBCL, and two had DLBCL associated with chronic inflammation. Over a median follow-up of 20 months (range, 2–31 months), the objective response rate was 83% (5/6) and the complete remission rate was 67% (4/6). No severe immune-related adverse reactions occurred, and only a mild rash was reported, which did not necessitate the discontinuation of therapy. Conclusion The combination of PD-1 inhibitors and chemotherapy offers promising results as a second-line treat‑ ment for patients with refractory EBV + DLBCL that is resistant to first-line immunochemotherapy regimens. These preliminary findings warrant further investigation in larger clinical trials to validate the efficacy and safety of this therapeutic approach.

Objective To evaluate the effects of low pneumoperitoneum pressure under deep muscle relaxation on inflammatory factors,oxidative stress and morphology of distal greater omental capillary endothelial cells in patients undergoing laparoscopic radical gastrectomy(LAG)for gastric cancer.Methods Sixty patients with LAG were selected from January to June 2022,45 males and 15 females,aged 40-75 years,BMI 18-30 kg/m2,ASA physical status Ⅰ or Ⅱ.Patients were randomly divided into two groups:low pneumoperitoneum pressure group(group PL)and the control group(group PH),30 patients in each group.Both groups were treated with deep muscle relaxation condition(PTC=1 or 2),and the pneumo-peritoneum pressure was set at 10 mmHg in group PL,and 14 mmHg in group PH.The duration of pneumo-peritoneum maintenance,surgical time,anesthesia time,extubation time,PACU stay time,postoperative hospitalization time,the number of effective PCIA compressions and total PCIA compressions within 48 hours after surgery,and number of relief analgesia were recorded.The plasma concentrations of interleukin-1 β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α),contents of malondialdehyde(MDA),superoxide dismutase(SOD),and reduced glutathione(GSH)were recorded 5 minutes before induction,5 minutes before abdominal closure,and 24 hours after surgery.The morphology of capillary en-dothelial cells in the omental membrane was observed 5 minutes after opening abdomen and 5 minutes before abdominal closure.Results Compared with group PH,the number of effective PCIA compressions and total PCIA compressions in group PL were significantly reduced within 48 hours after surgery(P＜0.05),the concentration of IL-6 was significantly decreased in group PL 5 minutes before abdominal closure and 24 hours after surgery(P＜0.05),the content of MDA was significantly decreased in group PL 5 minutes be-fore abdominal closure(P＜0.05).The endothelial cell of the greater omental capillaries was slightly edematous and unevenly thick,while the nuclear contractile function was slightly weakened and the vascular lumen was moderately narrowed in group PL 5 minutes before abdominal closure.By contrast,the group PH showed obvious swelling of the endothelial cells with significant intracellular fluid accumulation and severe edema,while the vascular lumen turned to be extremely narrow and the nucleus became severe swelling.Conclusion The use of deep muscle relaxation with low abdominal pressure at 10 mmHg in laparoscopic surgery for gastric cancer can diminish inflammation and oxidative stress,alleviating postoperative pain,re-duce the pneumoperitoneum pressure-induced damage on capillary endothelial cells,and leading to better early postoperative recovery.

The purpose of this study was to clone and characterize the ZFP36L1 (zinc finger protein 36-like 1) gene, clarify its expression characteristics, and elucidate its expression patterns in different tissues of goats. Samples of 15 tissues from Jianzhou big-eared goats, including heart, liver, spleen, lung and kidney were collected. Goat ZFP36L1 gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR), then the gene and protein sequence were analyzed by online tools. Quantitative real-time polymerase chain reaction (qPCR) was used to detect the expression level of ZFP36L1 in intramuscular preadipocytes in different tissues and adipocytes of goat at different differentiation stages. The results showed that the length of ZFR36L1 gene was 1 224 bp, and the coding sequence (CDS) region was 1 017 bp, encoding 338 amino acids, which was a non-secretory unstable protein mainly located in nucleus and cytoplasm. Tissue expression profile showed that ZFP36L1 gene was expressed in all selected tissues. In visceral tissues, the small intestine showed the highest expression level (P < 0.01). In muscle tissue, the highest expression level was presented in longissimus dorsi muscle (P < 0.01), whereas the expression level in subcutaneous adipose tissue was significantly higher than that in other tissues (P < 0.01). The results of induced differentiation showed that the expression of this gene was up-regulated during adipogenic differentiation of intramuscular precursor adipocytes (P < 0.01). These data may help to clarify the biological function of the ZFP36L1 gene in goat.
Animals ; Goats/genetics* ; Amino Acid Sequence ; Liver ; Cloning, Molecular