BACKGROUND: Among the more than 300 mycotoxins that are known to have toxic effects on animals and humans, Fusarium toxins deoxynivalenol (DON), T-2 and HT-2 toxins (T2/HT2), and zearalenone (ZEN) are frequently detected in domestic agricultural products. This study aimed to assess DON, T2/HT2, and ZEN exposure in Japanese adults by measuring urinary mycotoxins, observing their distributions, and making comparisons with data from other countries. METHODS: A total of 201 individuals participated in the study. Twenty-four-hour urine samples were collected from young adults (34 men and 35 women) in the Tokai region (urban area) and spot urine samples were collected from middle-aged and elderly adults (64 men and 68 women) in the Donan area of Hokkaido Prefecture (rural area). Urinary DON, T2/HT2, and ZEN levels were measured using a validated liquid chromatography-tandem mass spectrometry method. RESULTS: For DON, T2/HT2, and ZEN, the detection frequencies above the limit of detection (LOD) level (0.15, 0.13, and 0.01 µg/L, respectively) in all the samples were 53%, 26%, and 71%, respectively. The median concentrations (95^th percentile) of urinary DON, HT2, and ZEN were 0.19 (3.93), CONCLUSIONS This study represents the first comprehensive exposure assessment for DON, T2/HT2, and ZEN in Japanese adults using human biomonitoring methods. These data provide valuable information for a better understanding of mycotoxin exposure in Japan.
Humans ; Zearalenone/urine* ; Japan ; Male ; Female ; Trichothecenes/urine* ; T-2 Toxin/urine* ; Biological Monitoring ; Adult ; Middle Aged ; Aged ; Cross-Sectional Studies ; Young Adult ; Mycotoxins/urine* ; Environmental Exposure/analysis* ; East Asian People

Objective: In hospitals, the most recent medical information paper delivered by the hospital pharmacy is essential for obtaining information about the newest therapies and/or avoiding adverse effects of medicines.  To improve the medical information paper (hereafter, Medical News), we investigated the dissemination of Medical News delivered monthly by the Pharmaceutical Department in Oami Hospital.
Methods: Questionnaires about Medical News were distributed to each occupational category working in Ohami Hospital, and collected during June and July 2010.
Results: Responses were obtained from 127, including 19 doctors (full-time and part-time).  Ninety (70.9%) knew about Medical News, and 75 (59.1%) had read it.  It was revealed that there were differences in the familiarity with to Medical News among job classifications, and articles described on the same Medical News.
Conclusion: It insufficient to satisfied to provide drug information as a one-way provision by Medical News; however, as there are differences in the information required for each job, it is difficult to deliver a large number of copies in the small-scale hospital.  Overlooking the most up-to-date information about medicine and medical devices can lead to serious problem.  We have to review the information and provide important and accurate drug information to the staff in our hospital.

[Objective]It is important for students to learn in school education how to prevent medical accidents caused by deep insertion of a needle. Also, it is essential in safe acupuncture treatment that students are always reminded there are individual variations in subcutaneous structure and to confirm the position of the needle tip in the subcutaneous tissue. To recognize the acupuncture needle tip, muscle twitch induced by electrical stimulation through the acupuncture needle tip was felt with fingers of training students.
[Methods]To prevent accidental pneumothorax, acupuncture training was performed in the upper and lower extremities. After the class, we conducted a questionnaire investigation with the students.
[Result]As the result, students realized that the position of the needle tip which they assumed is different from the real position in subcutaneous tissue.
[Discussion]Without knowing the precise position of the acupuncture needle tip, a desirable effect is not provided, and furthermore, a medical accident may be caused. It is important to confirm the position of the acupuncture needle tip with consideration to individual variation for safe and effective acupuncture treatment.
[Conclusion]We concluded that the skill to prick and insert the acupuncture needle to the correct position is so important in preventing medical accidents that training to learn correct needle tip position should be given in acupuncture education.

OBJECTIVESNormal activity monitoring methods are mainly useful for relatively healthy and ablebodied people, but are not necessarily appropriate for elderly persons who may have difficulty in walking, or for the frail who may be bedridden. The purpose of this study was to examine 24-hour heart rate recording for the comparison of activity levels in daily life of elderly persons of different ambulatory abilities.

METHODSForty-two elderly females (mean, 82.1 years old) volunteered to participate in this study. The subjects were divided into four groups on the basis of their ambulatory status, and their 24-hour heart rate recordings were compared with their results of activity assessments.

RESULTSThe results of activity assessments showed a tendency to decrease as the ambulatory ability of the group decreased. The "total heart beats", calculated as the sum of all heart rates over 24 hours, were almost the same among the four groups, and therefore did not show a similar tendency. However, the "total excess-beats product (TEBP)" correlated with the results of activity assessments. TEBP was calculated as the sum of all differences in beats between each heart rate in 24 hours and the mean heart rate during sleeping at night. Therefore, TEBP may reflect a more active state than the bedridden state.

CONCLUSIONSThese results suggest that comparison of activity levels in daily life using 24-hour heart rate recording might be possible by the calculation of TEBP, and that this method might be useful for the comparison of the activity levels of elderly persons of diffent ambulatory abilities.

Treating atopic dermatitis patients, who have used steroid, only through Chinese herb medication, it often happens that extract medicine doesn't work well and infusion one is needed.
We treat with Seiun-haidoku-in-kagen “Ekisin-ittoku” and its amount, to stop using steroid, often exceeds as twice or three times much as ordinary use. Especially Sekko needs large quantities, 40-100g a day for adults to get out of steroid.
In early treatments, some patients couldn't stop applying steroid because of insufficient increase of Sekko, but after increasing its amount until it have effects, they all have got out of steroid.
It is effective to take herb-medicated bath and some original ointment at the same time. We report on the case in which one can withdraw from steroid by these treatments.

The establishment of experimental peripheral neuropathy and effect of moxibustion on it have been studied using male S. D. rats. To determine the condition of experimental peripheral neuropathy, rats were fed on the solution of acrylamide in the concentration of 100, 200, 300, 400 and 500p.p.m. The body weight decreased dependently on its concentration and the behavior in rats was also detriorated. Especially, ataxia, hindlimb weakness and muscle wasting in rats were remarkably observed.
The effect of moxibustion has been studied on the rats which fed on the 400p.p.m. acrylamide solution. The method of moxibustion is followes; 25mg of moxa was divided into 10 cones and they were treated by each 5 cones on right and left B-18, B-25 and G-34, respectively. The treatment of moxibustion was 6 times in 3 ones a week for 13 days.
As a results, after 12-13 days, moxibustion treated rats showed to improve the deterioration of behavior and muscle wasting induced by acrylamide. Especially, B-25 treated rats showed the improvement of weight in anterior tibial, gastrocnemius and soleus muscle. On the other hand, steadiness on slope was decreased by acrylamide feeding and decrease of the steadiness was not improved by the treatment of moxibustion.
It is suggested that the sutable stimulation of moxibustion improved the deterioration of behavior and muscle waste on the acrylamide induced peripheral neuropathy in rats.

Morphological changes of sciatic and tivial nerves have been observed microscopically and cholinesterase activities of anterior tibial, gastrocnemius and soleus muscles have been examined by the biochemical determination and histochemical method. 25mg of moxa/body (3 times a week, x6) were treated on the acupuncture point, B-18, B-25 and G-34 in acrylamide neuropathy rats.
After 400p.p.m. acrylamide treatment, the degeneration of peripheral nerves with myelin ovoids and foldes, and shrunken myelinated axons were observed. These lesions were advanced in the tibial nerve than in the sciatic nerve. In B-25 and B-18 moxibustion rats, good results were obtained to decrease myelin degeneration in peripheral nerves, but the lesions were advanced in G-34 rats than in acrylamide ones.
In the acrylamide rats, no change of muscle cholinesterase activity was found except the increase in the enzyme activity in the homogenate of anterior tibial muscle. In the gastrocnemius muscle of moxibustion treated rats, the microsomal fraction which contained much more sarcoplasmic reticulum showed high cholinesterse activity. And the activity per muscle protein showed various changes.
It showed that the stimulation of moxibustion had a mild effect to recover the experimental peripheral neuropathy induced by 400p.p.m. acrylamide because of morphological changes of sciatic and tibial nerves and weight of skeletal muscles.