Objective To develop novel metformin carbon dots(MCDs)with superior performance to enhance the osteogenic differentiation potential of human periodontal ligament stem cells(hPDLSCs)under inflammatory conditions.Methods Three types of MCDs-MCDsCA,MCDsAA,and MCDsCS were synthesized via a one-step hydrothermal method using metformin(Met)in combination with citric acid(CA),ascorbic acid(AA),and carboxymethyl chitosan(CS),respectively.Their physicochemical properties were characterized by transmission electron microscopy(TEM),Fourier-transform infrared spectroscopy(FTIR),and UV-visible spectrophotometry.The biocompatibility and cellular uptake of the MCDs were assessed using CCK-8 assay and confocal laser scanning microscopy.Alkaline phosphatase(ALP)staining and ALP activity assay were conducted to identify the most effective MCDs type in promoting osteogenic differentiation of hPDLSCs under inflammatory conditions.Subsequently,ALP and Alizarin Red S(ARS)staining,qRT-PCR,and Western blotting were performed to assess the effects of the optimal concentration of MCDsCS and its raw material(Met+CS)on osteogenic differentiation under inflammatory conditions.Results All 3 types of nanoscale MCDs exhibited favorable physicochemical characteristics and biocompatibility,and could be effectively internalized by hPDLSCs.Both MCDsCA and MCDsAA significantly promoted hPDLSCs proliferation at lower concentrations(0～0.50 mg/mL),while MCDsCS exhibited excellent cytocompatibility over a broader range(0～2.00 mg/mL).Under inflammatory conditions,MCDsCA and MCDsAA showed no significant effect on ALP expression in hPDLSCs,whereas MCDsCS significantly enhanced ALP expression in a dose-dependent manner,with the optimal effect observed at 0.10 mg/mL(P<0.05).Compared to Met+CS,0.10 mg/mL MCDsCS significantly enhanced ALP expression and calcium nodule formation under inflammatory conditions.It also upregulated the expression of osteogenesis-related genes(ALP,RUNX2,COL-1,OCN)and proteins(ALP,RUNX2,OCN)(P<0.01),while reducing the levels of inflammatory cytokines IL-1β and IL-6(P<0.01).Conclusion A novel MCDsCS with sound biocompatibility is successfully developed,and can effectively promote osteogenic differentiation of hPDLSCs and exerts anti-inflammatory effects under inflammatory conditions,indicating its potential as a nanotherapeutic agent for periodontitis-associated bone regeneration.

Objective:To investigate the association of WWP2 single nucleotide polymorphism (rs3790088, rs4247109) with delayed encephalopathy after acute carbon monoxide poisoning (DEACMP) , and explore the influences of DEACMP genetic predisposition. Methods:From November 2006 to December 2017, 235 DEACMP cases and 429 acute carbon monoxide poisoning (ACMP) cases were selected. All ACMP patients were followed up for more than 90 days without DEACMP. The DNA in all blood samples were extracted with the blood Genome DNA Extraction Kit. The method of Sequenom Mass Array SNP technique was used to detect the genotype and allele of WWP2. All DEACMP patients were assessed every 3 days after hospitalization by the Hasegawa Dementia Scale (HDS) and Activity of Daily Living Scale (ADL) . The distribution of genotypes in conformty with Hardy-Weinderg law was analyzed by goodness-of-fit χ 2 test, and χ 2 test was used for association analysis. Results:For rs3790088, there were 226 DEACMP cases and 414 ACMP cases. For rs4247109, there were 234 DEACMP cases and 428 ACMP cases. For rs3790088 and rs4247109 in WWP2 gene: there were not significant differences in the gene genotype distribution and allele frequency of both DEACMP group and ACMP group ( P>0.05) . According to gender, there were not significant differences in WWP2 gene genotype distribution and allele frequency between two female groups and two male groups ( P>0.05) . After analysis by genetic model, the genotype distributions in both DEACMP group and ACMP group were not significantly differences in three genetic models (codominant genetic model, recessive genetic model and dominant genetic model, P>0.05) . Conclusion:It has not confirmed the genetic correlation between the two gene single nucleotide polymorphisms (rs3790088, rs4247109) of WWP2 gene and the incidence of DEACMP.

Objective:To investigate the association of WWP2 single nucleotide polymorphism (rs3790088, rs4247109) with delayed encephalopathy after acute carbon monoxide poisoning (DEACMP) , and explore the influences of DEACMP genetic predisposition. Methods:From November 2006 to December 2017, 235 DEACMP cases and 429 acute carbon monoxide poisoning (ACMP) cases were selected. All ACMP patients were followed up for more than 90 days without DEACMP. The DNA in all blood samples were extracted with the blood Genome DNA Extraction Kit. The method of Sequenom Mass Array SNP technique was used to detect the genotype and allele of WWP2. All DEACMP patients were assessed every 3 days after hospitalization by the Hasegawa Dementia Scale (HDS) and Activity of Daily Living Scale (ADL) . The distribution of genotypes in conformty with Hardy-Weinderg law was analyzed by goodness-of-fit χ 2 test, and χ 2 test was used for association analysis. Results:For rs3790088, there were 226 DEACMP cases and 414 ACMP cases. For rs4247109, there were 234 DEACMP cases and 428 ACMP cases. For rs3790088 and rs4247109 in WWP2 gene: there were not significant differences in the gene genotype distribution and allele frequency of both DEACMP group and ACMP group ( P>0.05) . According to gender, there were not significant differences in WWP2 gene genotype distribution and allele frequency between two female groups and two male groups ( P>0.05) . After analysis by genetic model, the genotype distributions in both DEACMP group and ACMP group were not significantly differences in three genetic models (codominant genetic model, recessive genetic model and dominant genetic model, P>0.05) . Conclusion:It has not confirmed the genetic correlation between the two gene single nucleotide polymorphisms (rs3790088, rs4247109) of WWP2 gene and the incidence of DEACMP.

An 8-month-old female infant received propranolol 2 mg orally twice daily and prednisone 7.5 mg orally once daily for kaposiform hemangioendothelioma.Due to poor efficacy,propranolol and prednisone were discontinued and sirolimus 0.2 mg was given orally twice daily.The child's liver function was normal before sirolimus treatment.On day 10 of sirolimus treatment,the laboratory tests showed alanine aminotransferase(ALT) 58 U/L,aspartate aminotransferase(AST) 116 U/L,γ-glutamyltransfarase(γ-GT) 84 U/L,alkaline phosphatase(ALP) 181 U/L,total bile acid(TBA) 109.2 μmol/L,total bilirubin(TBil) 57.4 μmol/L,and direct bilirubin(DBil) 38.0 μmol/L.At the same time,the child developed decrease of appetite,milk refusal,and cry and scream all the time.The physical examination showed slightly yellow on her skin and sclera.Cholestatic liver injury caused by sirolimus was considered.Sirolimus was discontinued and liver-protective therapy and drugs for anti-jaundice were given.On day 6 of drug withdrawal,the laboratory tests showed ALT 58 U/L,AST 116 U/L,γ-GT 84 U/L,ALP 181 U/L,TBA 109.2 μmol/L,TBil 41.5 μmol/L,and DBil 20.8 μmol/L;the child's appetite recovered,yellowish skin and sclera improved markedly.On day 20 of drug withdrawal,the child was discharged.No abnormalities were found in the child's liver function after 15 days of discharge.

A 65-year-old male patient received oral Anweiyang capsules 0. 4 g four times daily for chronic gastritis. About 1 month after the medication,the patient developed intermittent headache, dizziness,and increased blood pressure,whose highest value was 180 / 100 mmHg. Levamlodipine besylate tablets 2. 5 mg once daily was given orally but the patient′s blood pressure was not well controlled. About 4 months later,laboratory tests showed obviously increased free cortisol in urine (2330 nmol/ 24 h),which was considered to be caused by Anweiyang capsules. Anweiyang capsules were stopped and the patient continued to take levamlodipine besylate tablets. On day 4 after the drug withdrawal,the patient′s blood pressure and urinary free cortisol began to decrease,which finally returned to normal on day 9. At 1 month of follow-up,no abnormalities were found on the blood pressure and urinary free cortisol in the patient.

An 8-month-old female infant received propranolol 2 mg orally twice daily and prednisone 7.5 mg orally once daily for kaposiform hemangioendothelioma.Due to poor efficacy,propranolol and prednisone were discontinued and sirolimus 0.2 mg was given orally twice daily.The child's liver function was normal before sirolimus treatment.On day 10 of sirolimus treatment,the laboratory tests showed alanine aminotransferase(ALT) 58 U/L,aspartate aminotransferase(AST) 116 U/L,γ-glutamyltransfarase(γ-GT) 84 U/L,alkaline phosphatase(ALP) 181 U/L,total bile acid(TBA) 109.2 μmol/L,total bilirubin(TBil) 57.4 μmol/L,and direct bilirubin(DBil) 38.0 μmol/L.At the same time,the child developed decrease of appetite,milk refusal,and cry and scream all the time.The physical examination showed slightly yellow on her skin and sclera.Cholestatic liver injury caused by sirolimus was considered.Sirolimus was discontinued and liver-protective therapy and drugs for anti-jaundice were given.On day 6 of drug withdrawal,the laboratory tests showed ALT 58 U/L,AST 116 U/L,γ-GT 84 U/L,ALP 181 U/L,TBA 109.2 μmol/L,TBil 41.5 μmol/L,and DBil 20.8 μmol/L;the child's appetite recovered,yellowish skin and sclera improved markedly.On day 20 of drug withdrawal,the child was discharged.No abnormalities were found in the child's liver function after 15 days of discharge.

A 65-year-old male patient received oral Anweiyang capsules 0. 4 g four times daily for chronic gastritis. About 1 month after the medication,the patient developed intermittent headache, dizziness,and increased blood pressure,whose highest value was 180 / 100 mmHg. Levamlodipine besylate tablets 2. 5 mg once daily was given orally but the patient′s blood pressure was not well controlled. About 4 months later,laboratory tests showed obviously increased free cortisol in urine (2330 nmol/ 24 h),which was considered to be caused by Anweiyang capsules. Anweiyang capsules were stopped and the patient continued to take levamlodipine besylate tablets. On day 4 after the drug withdrawal,the patient′s blood pressure and urinary free cortisol began to decrease,which finally returned to normal on day 9. At 1 month of follow-up,no abnormalities were found on the blood pressure and urinary free cortisol in the patient.

OBJECTIVEBy explore the role of serum soluble Fas (sFas) in occurrence and progression of delayed encephalopathy after acute carbon monoxide poisoning (DEACMP).

METHODSEnzyme-linked immunosorbent assay (ELISA) was used to detect serum sFas levels in 40 patients with DEACMP in acute stage and convalescent stage, with 36 healthy elderly subjects as the control group.

RESULTSSerum sFas levels of the patients with DEACMP in both the acute and convalescent stages showed no significant difference from those in the control group (P=0.737 and 0.137, respectively), nor was any significant difference found between the patients in acute and exacerbation stages (P=0.059).

CONCLUSIONSerum sFas is not involved in the occurrence and progression of DEACMP.

Adult ; Aged ; Aged, 80 and over ; Brain Diseases ; etiology ; Carbon Monoxide Poisoning ; blood ; complications ; Case-Control Studies ; Female ; Humans ; Male ; Middle Aged ; fas Receptor ; blood

ObjectiveTo explore puncture point and angle of skin for unipedicular vertebroplasty by imaging measurement and combining clinical practice in order to pr vide reference for clinical work.MethodsCT images of thoracic and lumbar vertebrae of imaged with multislice helical CT were random chosen.From high to low position vertebrae of easy fracture T6 to -L5,its angle between vertebraI pedfcle stalk line and 1/3 of vertebraI body stalk line fore and median of point of intersection was measured.The angle of sternocostal joints route was also measured.At the same time the distance between site of skin puncture and spinous process was measured.ResultsThe angle between the thoracic vertebraI pedicle and sagittal section was (28.2 ± 3.8) °,the lateral distance of the spinous process was ( 31.6 ± 5.4) mm.The angle of thoracic vertebraI pedicle lateral route was (35 ± 3.4) °,the distance was ( 55.2 ± 5.3 ) mm.In the lumbar vertebrae pedicle route,the angle of L1 -L4 was (29.3 ± 5.6 ) °,the distance was (38.4 ± 4.5 )mm.Ls was (39.2 ± 3.3) °,the distance was ( 71.6 ± 6.2) mm.Combining clinical practice,the lateral distance of the spinous process in thoracic vertebraIpedicle route,the best puncture point was 30 mm,in thoracic vertebraI pedicle lateral route it was 40 mm,in L1-L4 it was 45 mm,in L5 it was 60 mm.ConclusionsThe unipedicular vertebroplasty can be performed rapidly,accurately and safely,and it reduces complications,cost and time.

Objective To investigate the surgical treatment and indications for primary lesion in patients with stage Ⅳ rectal cancer.nethods Clinical data of 118 patients with stage Ⅳ rectal cancer undergoing surgical treatment from January 1988 to December 2005 in Cancer Hospital of Chinese Academy of Medical Sciences were analyzed retrospectively using Statistical Package for the Social Sciences(Release 13.0,SPSS,Inc).Survival was estimated using the Kaplan-Meier method and compared using the log-rank test.Cox regression was used in multivariate analysis.Results One hundred and five of 118 patients received rectal primary lesion resection,including 16 cases with simultaneously metastatic tumor resection.13 of 118 patients received ostomy.The overall 5-year survival rate of the primary lesion resection group was 8.57%,the 5-year survival rate of the metastatic tumor resection group was 31.2%.The median survival in the primary lesion resection group and the ostomy group was 15 months and 13 months respectively(X2=0.736.P=0.778).Multivariate analysis showed that degree of differentiation of primary tumor,maximal diameter of liver metastasis and chemotherapy were the most important prognostic factor of the primary tumor resection group.Conclusion Simultaneous resection of primary and metastatic tumor can prolong suivival time of patients with resectable stage Ⅳ rectal cancer.