Practice-oriented education is an important link in cultivating qualified builders and reliable successors of socialism， and it is particularly important for cultivating high-quality talents in traditional Chinese medicine （TCM）. Practice-oriented education is a vital part in improving college students’ ideological and political literacy， an important measure for TCM colleges and universities to promote the inheritance and innovation of TCM culture， and a significant pathway to cultivate high-quality TCM talents. The prominent issues of practice-oriented education in TCM colleges and universities are that the small ideological and political classroom is not closely integrated with the large classroom of society， the characteristics of TCM culture are not highlighted， and the current situation of practice-based construction does not match the needs of talent cultivation. Based on systematic thinking， the construction of a collaborative education of TCM culture and red culture should start from the following four aspects： cultural integration-cultivating ideological and political education and ideological and political theory courses of TCM culture； medical and educational collaboration-leveraging the synergistic educational effect of clinical practice； practice reinforcement-integrating the small classroom of ideological and political education into the big classroom of society； and “double innovation” promotion-creating an educational environment for innovation and entrepreneurship of TCM.

Objective To investigate the safety and efficacy of sodium fluorescein-guided microsurgery in children with medulloblastoma,and to analyze the surgical efficacy and prognosis.Among them,12 cases underwent unilateral telovelar approach,2 cases underwent bilateral telovelar approach,and 6 cases underwent telovelar approach combined with transvermian approach.The intraoperative dose of sodium fluorescein was 2 mg/kg.Methods The clinical data of 20 patients with medulloblastoma treated with fluorescein sodium assisted microsurgery from January 2018 to August 2023 in Xinjiang Autonomous Region People's Hospital were retrospectively analyzed.Results Of the 20 patients,12 were male and 8 were female.The mean age of onset was(7.9±3.7)years.In all cases,there was clear tumor fluorescence,none of the cases had adverse reaction associated with the use of sodium fluorescein.There were 16 cases of gross-total resection,3 cases of near-total resection,1 case of partial resection,1 case of intracranial infection,2 case of subcutaneous effusion,2 cases of cerebellar mutism.The follow-up time was from 3 to 72 months.5 cases did not receive sufficient radiotherapy and chemotherapy,and 9 cases died due to tumor progression or recurrence.In all cases,the longest overall survival was 72 months,the mean survival time was 39.2 months,and the median survival time was 41.2 months.Conclusion Fluorescein sodium assisted microsurgery is safe and effective in pediatric medulloblastoma surgery.

Objective To investigate the targeting of transforming growth factor β1(TGF-β1)expression by Ran-binding protein 9(RANBP9)and its effect on colorectal cancer Colo320 cell apoptosis.Methods Gene expression levels of RANBP9 were analyzed in 625 colon cancer tissues and 20 normal colon tissues in The Cancer Genome Atlas database.The relationship between RANBP9 expression and survival time of patients with colon cancer was analyzed using KMPLOT.The expression of TGF-β1 in normal colon tissues and colon cancer tissues was analyzed using the human protein immunohistochemistry database and the relationship between TGF-β1 expression and the survival of patients with colon cancer was analyzed using the UALCAN database.The relationship between RANBP9 and TGF-β1 was analyzed by dual luciferase experiments.Colo320 cells were transfected with pcDNA3.1-GFP-RANBP9 and control pcDNA3.1-GFP-RANBP9-NC plasmids,respectively,and normal control cells were established without transfection.The cells were cultured and the growth viability of each group of cells was detected by the iazolyl blue tetrazolium bromide method,apoptosis was detected by flow cytometry,the mitochondrial membrane potential was detected by JC-1 staining,and RANBP9 and TGF-β1 protein expression were detected by Western blot.Results RANBP9 expression was significantly reduced in colon cancer tissues.Compared with patients with low RANBP9 expression,patients with high RANBP9 expression had a higher survival curve and significantly higher expression of TGF-β1 in colon cancer tissue.Compared with patients with high TGF-β1 expression,patients with low TGF-β1 expression had a significantly higher survival curve(P＜0.05).RANBP9 targeted the expression of TGF-β1 in colon cancer.Compared with the normal group,cell growth,mitochondrial membrane potential,and TGF-β1 expression were all significantly down-regulated and the apoptosis rate and RANBP9 expression were significantly increased in the experiment group(P＜0.05).Conclusions RANBP9 can target the expression of TGF-β1,promote the growth of Colo320 colon cancer cells,decrease the mitochondrial membrane potential,and induce apoptosis.

Objective:To investigate the potential effect and mechanism of docosahexaenoic acid(DHA) on hippocampal neuronal apoptosis and c-Jun N-terminal kinase(JNK) protein in rats with subarachnoid hemorrhage(SAH).Methods:Forty eight SPF-grade male SD rats were randomly divided into control group, sham group, model group and DHA intervention group according to the random number table method, with 12 rats in each group.The rats in model group and DHA group were injected with autologous blood(0.3 mL) into the optic chiasma to establish the SAH model.Rats in model group were intraperitoneally injected with DHA(35 mg/kg) 3 hours after SAH model establishment, rats in sham operation group were injected with 0.3 mL 0.9% sodium chloride solution into the optic chiasma, and rats in control group were fed normally.Neurobehavioral function of all rats was evaluated after 24 hours.The apoptosis of neuron was observed by TUNEL staining, and the expression of phosphorylated JNK(p-JNK)and apoptosis-related proteins Bax and Bcl-2 was observed by Western blot.Statistical analysis was performed using GraphPad Prism 7.0 software.One-way ANOVA was used for comparison among multiple groups, and Tukey test was used for further pairwise comparison.Results:(1)The differences in neurobehavioral function scores among the 4 groups of rats were statistically significant( F=103.60, P<0.05), the neurobehavioral function scores in model group(8.67±1.37) and DHA intervention group(13.67±1.51) were lower than that in control group(18.00±0.00) and sham group(17.67±0.52)( all P<0.05), while the neurobehavioral function score in DHA intervention group was higher than that in the model group( P<0.05).(2)The results of TUNEL staining showed that there were statistical differences in the number of hippocampal neuron apoptosis among the 4 groups( F=30.76, P<0.05), the number of hippocampal neuron apoptosis in model group(55.67±5.28) was higher than those in control group(25.83±7.06) and sham group(25.50±6.72) (both P<0.05), the number of hippocampal neuron apoptosis in DHA intervention group(35.17±5.78) was lower than that in model group.(3)The results of Western blot showed that there were no statistical differences in the Bax protein levels among the four groups( F=2.00, P>0.05).There were statistical differences in the expression levels of Bcl-2, p-JNK and Bax/Bcl-2 ratio among the 4 groups( F=8.48, 5.69, 5.39, all P<0.05).There was no statistical difference in Bcl-2, p-JNK protein levels and Bax/Bcl-2 ratio between the control group and sham group(all P>0.05).The p-JNK protein levels and Bax/Bcl-2 ratio in model group ((1.93±0.25), (2.05±0.86)) were higher than those in sham group ((1.42±0.33), (1.05±0.26)) (both P<0.05), the Bcl-2 protein level in model group (1.04±0.23) was lower than that in sham group (1.61±0.16) ( P<0.05).The p-JNK protein level and Bax/Bcl-2 ratio in DHA intervention group((1.43±0.33), (1.19±0.30)) were lower than those in model group(both P<0.05), the Bcl-2 protein level in DHA intervention group(1.42±0.28) was higher than that in model group( P<0.05). Conclusion:DHA can reduce neuronal apoptosis, inhibit the activation of p-JNK and improve neurological function of SAH model rats.

Objective To standardize nursing management on postoperative patients with a left ventricular assist-ant device(LVAD).Methods The first draft of the Consensus was formed on the basis of literature review.2 rounds of expert consultations and a round of online meeting discussion were held for adjustments and modifications the draft of the Consensus.Results The recovery rate of the inquiry questionnaire was 93.75%.The authority coefficients of the 2 rounds of inquiry experts were 0.927 and 0.920.The concentration degree of expert opinions for each indicator was greater than 3.5 score,and the coefficient of variation was less than 0.25.The Kendall harmony coefficients for 2 rounds of correspondence were 0.402 and 0.407(P<0.01).The final Consensus formed through expert consultations and meetings includes 7 themes:hemodynamic monitoring,LVAD function monitoring,coagulation function monitoring,percutaneous cable and wound care,exercise rehabilitation care,health education and guidance,and pre-discharge assessment.Conclusion The Consensus is scientific,rigorous,and authoritative.The Consensus covers all aspects of postoperative care for patients with LVAD,and it will benefit to clinical practice.

By analyzing information such as the number of publications，authors，institutions and keywords in the literature on the integration of traditional Chinese medicine（TCM）culture into ideological and political education in the past 10 years，it was found that the overall number of publications showed an increasing trend. As the main research fronts，TCM colleges and universities had not yet formed teams in cooperation with other universities. The research hotspots were relatively concentrated，but the research horizons were not broad enough. It is suggested that in the future，policy should be taken as the guide to build a cross-regional interdisciplinary cooperation system，horizontally and vertically extend research subjects，innovate and construct diversified teaching models，continue to deeply explore and organically integrate ideological and political education elements，continuously improve the evaluation system of educational functions，and further enhance the timeliness of integrating traditional Chinese medicine culture into ideological and political education. So as to make greater contributions to cultivating talents in the new era and promoting the inheritance and innovation of TCM culture.

Objective To investigate the current situation and health effects of indoor microbial pollution in Xi'an, to analyze the purification effect of air purifiers on indoor microbial pollution, and to provide reference for improving the indoor environment. Methods Through stratified random sampling, 20 families from rural areas and 20 families from upwind and downwind urban areas respectively were selected from Xi'an. Data was collected by questionnaire surveys and on-site environmental sampling. Non-parametric analysis and correlation analysis were used for statistical analysis. Results Overall, the standard-exceeding rate of total count of bacteria was 5.00%. The medians of the total count of bacteria and fungi were 312.50 cfu/m3 and 260.00 cfu/m³, respectively. In terms of health effects, the correlation between rhinitis and cold with total bacterial count was statistically significant (P<0.05), with the correlation coefficients of 0.182 and 0.223, respectively. Purification effect of air purifiers on microbial pollution was statistically significant (P<0.05). After opening for 2 hours, the total numbers of bacteria and fungi decreased significantly. Conclusion The occurrence risk of colds and rhinitis is increased by indoor microbial pollution. Air purifiers have a certain effect on decreasing the total number of bacteria and fungi. It is recommended to use air purifiers with high CADR of particulate matter, double-layer filter and sterilization and dehumidification function, and replace the filters regularly to reduce indoor microbiological contamination.

Objective To compare the analgesic efficacy of combined ultrasound-guided thoracic paravertebral block(TPVB)with patient-controlled intravenous analgesia(PCIA)versus simple PCIA after open partial hepatic resection.Methods A total of 120 patients undergoing elective open partial liver resection in our hospital from September 2020 to September 2022 were prospectively recru ited,and then randomly divided into the TPVB+PCIA group(Group A)and simple PCIA group(Group B),with 60 patients in each group.Finally,there were 112 patients meeting the criteria and completing statistical analysis.The patients in Group A underwent TPVB at T8～T9 in the left lateral position before anesthesia induction,with injection of 0.33％30 mL ropivacaine.The patients in Group B had no above treatment.Both groups were given PCIA analgesia pump after surgery.The main outcome measures were analgesia levels at 4,8,12,24 and 48 h after surgery and VAS pain scores at rest and activity.The secondary outcome measures included mean ventricular rate and mean arterial pressure during operation,time required for the first postoperative analgesia relief,number of patients for the first postoperative analgesia relief 48 h after the operation,time for the first postoperative ground exercise,time for postoperative anal exhaust,total number of postoperative hospital days,and incidence of postoperative adverse reactions.Results Compared with the patients of Group B,those of Group A had significantly lower scores of resting VAS and exercise VAS scores at 4,8,12 and 24 h after operation(P＜0.001),decreased mean heart rate and mean arterial blood pressure during operation(P＜0.01),less cumulative consumption of tramadol within 48 h(P=0.018),extended time for first postoperative analgesic need(P＜0.001),decreased average number of PCIA effective compressions and number of relief analgesia(P＜0.001),and advanced time of first ground movement and anal exhaust(P＜0.001).But,there were no significant differences in VAS scores at 48 h after surgery,total length of postoperative hospital stay and incidence of postoperative adverse reactions between the 2 groups.Conclusion Combined TPVB+PCIA shows better analgesic effect than simple PCIA for open partial hepatectomy,and it can obtain more stable intraoperative circulation and promote rapid postoperative recovery.

Objective:To establish a risk prediction model for prolonged mechanical ventilation in adult heart transplant recipients.Methods:This study used structured language to query hospital electronic medical record systems. From January 2004 to December 2021, 957 adult recipients undergoing cardiac transplantation at Fuwai Hospital of the Chinese Academy of Medical Sciences were selected as the study subject using a convenience sampling. This study collected basic information, past history, preoperative blood creatinine, preoperative total bilirubin, preoperative ventilation assistance, and intraoperative donor heart cold ischemia time. The enrolled patients were divided into a training group ( n=717) and a validation group ( n=240) in a ratio of 3∶1. Based on the training group data, a risk prediction model was constructed using two-way stepwise Logistic regression. The prediction performance of the model was evaluated using the area under the receiver operating characteristic (ROC) curve, sensitivity and specificity based on the validation group data. Results:A total of 957 adult heart transplant recipients were included, and 18.39% (176/957) of them experienced prolonged mechanical ventilation (>24 hours) after surgery. Based on the model established by the training group data, 7 independent risk factors for prolonged mechanical ventilation after surgery were clarified, including age ≥ 60 years old ( OR=1.820), history of blood transfusion ( OR=5.237), previous cardiac surgery ( OR=2.826), preoperative total bilirubin≥ 34.2 μmol/L ( OR=1.861), preoperative ventilator assistance ( OR=9.421), preoperative intra-aortic balloon pump assistance ( OR=1.826), intraoperative donor heart cold ischemia time≥ 360 min ( OR=2.093) ( P<0.05). The area under the curve of the model was 0.676 (95% confidence interval was 0.581 to 0.770), with a sensitivity of 0.500, and a specificity of 0.796. Conclusions:Based on the data of adult heart transplant recipients from a single center, this study established a relatively simple and objective risk prediction model that can predict the prolonged mechanical ventilation in adult heart transplant recipients, and has good clinical value.

Objective:To establish the sensitive quality indicators for cardiovascular nursing based on the RAND Corporation University of California, Los Angeles (RAND-UCLA) expert consensus method, so as to lay the foundation for scientific and standardized quality management of cardiovascular nursing.Methods:Relevant literature was searched on China National Knowledge Infrastructure, WanFang Data, VIP, China Biomedical Literature Database, Cochrane Library, PubMed, EMbase and CINAHL. The search period was from database establishment to August 31, 2021. We used the Johns Hopkins evidence-based nursing practice model for evidence-based analysis and summary, and referred to documents on cardiovascular nursing quality indicators to form an indicator pool. From May 23 to July 12, 2022, 17 experts were selected to apply the RAND-UCLA expert consensus method to inquire about the indicators.Results:The effective response rates of the first and third rounds of the questionnaire were both 100.00% (17/17) , and the authority coefficients of the experts were 0.87 and 0.88, respectively. Among the 18 indicators in the third round of expert consultation, the consistency degree of importance and operability of all indicators was less than 1.00. Finally, 18 sensitive quality indicators for cardiovascular nursing were established.Conclusions:The consistency and coordination of experts in this study are good, and the nursing sensitive indicators formed have scientific characteristics, cardiovascular specialty characteristics, and good operability.