Objective:To evaluate the clinical efficacy of the Magpie-bridge Microskin Grafting (hereinafter briefly referred to as Magpie-bridge surgery) in treating linear white scars (LWS).Methods:This study was a retrospective cohort study. From October 2022 to December 2023, 37 LWS patients were treated with the Magpie-bridge surgery at the Department of Plastic and Reconstructive Surgery of Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, including 9 males and 28 females, aged 25 (17, 36) years. All scars were <2 mm in width and 1–10 cm in length before surgery. Magpie-bridge surgery procedure: an electric punch was used to excise scar tissue at intervals to form wounds and harvest microskin grafts of the same size and thickness as the removed scar tissue from behind the ear or axillary apex. The microskin grafts were implanted at the wound sites and fixed with tension-reducing adhesive tape. The donor area wounds were treated with routine dressing changes. Twelve months after the first surgery, the efficacy was evaluated based on the degree of reduction in scar white area compared with that before the first surgery, and the treatment effectiveness rate was calculated. Before the first surgery and 12 months after the first surgery, the melanin scores of the normal skin area surrounding the scar and the scar area were evaluated using a skin imaging analysis system, and the difference between the two was calculated. Six of the aforementioned patients requested a second Magpie-bridge surgery in pursuit of better therapeutic effects. The white scar tissue left untreated during the first surgery (hereinafter referred to as untreated scar tissue) and the tissue from the site at 12 months post scar removal and microskin transplantation (hereinafter referred to as the recipient skin tissue at 12 months after the first surgery) were collected. The tissue structure, melanin quantity and distribution were examined by using hematoxylin eosin staining and Masson-Fontana staining, and the activity of tyrosinase positive melanocytes was observed by using immunofluorescence staining.Results:At 12 months after the first surgery, the results of efficacy evaluation showed that 24 cases were cured, 11 cases were improved, 1 case was ineffective, and 1 case was in a worsened condition, yielding a 94.6% (35/37) treatment effectiveness rate. The melanin score difference between the surrounding normal skin area of scar and the scar area was 0.45 (0.10, 1.65) at 12 months after the first surgery, which was significantly less than 2.50 (1.40, 5.96) before the first surgery ( Z=-5.02, P<0.05). Six patients had untreated scar tissue with flat epidermis and a flat junction between dermis and epidermis; the collagen fiber bundles in the dermis were thick and unidirectionally parallel; no skin appendages such as hair follicles were observed; the basal layer of the epidermis showed deposition of melanin particles, but no extensive depigmentation was observed. Compared with those of untreated scar tissue, the epidermal thickness increased, and epidermal protrusions appeared at the junction of dermis and epidermis of the recipient skin tissue at 12 months after the first surgery; hair follicles and sebaceous glands were visible, and collagen fibers in the dermis were arranged vertically and horizontally in an orderly manner. Melanin particles were deposited in the basal layer of the epidermis, and the melanin content per unit area of tissue was increased. Tyrosinase-positive melanocytes in untreated scar tissue and in the recipient skin tissue at 12 months after the first surgery were mainly located at the basal layer of the epidermis, with normal cell activity and no significant difference. Conclusions:The Magpie-bridge surgery can significantly improve the appearance of LWS in patients, with definite therapeutic effects and value for clinical promotion; the improvement of LWS appearance by Magpie-bridge surgery may be related to the increase of melanin content per unit area of tissue and the normalization of tissue structure.

Objective To assess the clinical value of a novel surgical technique—Tubeless subxiphoid uniportal video-assisted thoracoscopic surgery with percutaneous suspension technique via balance-shaped sternal elevation device in the resection of anterior mediastinal masses. Methods Patients who underwent tubeless subxiphoid uniportal video-assisted thoracoscopic surgery via balance-shaped sternal elevation device in anterior mediastinal masses process at the Department of Thoracic Surgery, West China Hospital, Sichuan University from March to April 2025 were included, and their clinical data were analyzed. Results A total of 4 patients were included, with 2 males and 2 females, aged 58-75 years. The diameter of the tumor was 2.5-3.0 cm. The operation time was 60.0-150.0 min, intraoperative blood loss was 5-10 mL, pain score on the 3rd day after surgery was 0 points, and postoperative hospital stay was 2-3 days. All patients achieved complete resection of the masses and thymus without perioperative complications. Conclusion The tubeless subxiphoid uniportal video-assisted thoracoscopic surgery with percutaneous suspension technique via balance-shaped sternal elevation device technique optimizes surgical visualization and instrument maneuverability while avoiding complications related to conventional anesthesia and tubing, thereby markedly enhancing the minimally invasive profile of anterior mediastinal masses resections. In addition to maintaining procedural safety, this approach effectively reduces postoperative pain and accelerates patient recovery, highlighting its potential for widespread clinical adoption.

Objective:To investigate the killing effect of the oncolytic virus Rigvir on six different colorectal cancer cell lines in vitro and differences in the sensitivity of different cell lines to Rigvir,to analyze the differentially expressed genes and signaling pathways be-tween sensitive and insensitive cells and the reasons for such differences,to explore the killing mechanism of the oncolytic virus Rigvir on colorectal cancer cells based on experimental results,and to lay a theoretical foundation for the use of the oncolytic virus Rigvir as a novel immunotherapeutic drug for the treatment of colorectal can-cer.Methods:Cell Counting Kit-8(CCK-8)assay was used for quantitative assessment of the inhibition rate of Rigvir on cells,and the Annexin V-FITC\PI method was used to measure the apoptosis rate of sensitive cell lines and preliminarily analyze its killing mechanism.Bioinformatics techniques were used to investigate the differentially expressed genes and signaling pathways between sensitive and insensitive cells,and Western blot experiments were used for validation and detecting the expression of apoptosis factors.High expression of upstream factors in differential signaling pathways,and application of real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR)and WB to detect the effect of Rigvir on the expression levels of target genes and proteins in overexpressing sensitive cell lines.Results:SW480 and HT29 were sensitive to the oncolytic virus Rigvir,others had relatively insensi-tive(P<0.001).However,the inhibitory effect of Rigvir with two concentration gradients on HT29 was more significant(P<0.001).After 48 hours of infection,the cell inhibition rate of SW480 cells no longer increased with the prolongation of infection time(F=52.010,P=0.147),but it was more sensitive to changes in virus concentration(F=13.490,P<0.001).On the contrary,changes in virus concentration had no significant effect on the inhibition rate of HT29 cells(F=8.450,P=0.281),but the inhibition rate continued to in-crease after 48 hours with the prolongation of infection time(F=24.380,P<0.001).The apoptosis rate of sensitive group cells gradually increased with the prolongation of infection time(P<0.001),which mainly characterized by late stage apoptosis and necrotic cells.Through bioinformatics techniques,significant differences were observed in the classic PI3K/Akt/mTOR signaling pathway between the sensitive and insensitive groups.Western blot experiments showed that after the application of Rigvir,the upstream protein expression of PI3K/Akt/mTOR in the sensitive cell group was significantly reduced(P<0.001).The expression levels of apoptosis factors caspase-3 and caspase-8 increased,while the Bcl-2/Bax ratio decreased(P<0.001).The results of RT-qPCR and Western blot showed that after Rigvir was applied to the sensitive cell line HT29 overexpression type,the expression levels of PI3K/Akt/mTOR upstream and downstream signaling molecules(Akt,4EBP1,and p70S6K)were significantly reduced compared to the control group(P<0.05).Conclusion:Rigvir can effectively kill some colorectal cancer cell lines(SW480,HT29)in vitro,its mechanism of action is partially in-duced by the PI3K/Akt/mTOR classical signaling pathway to induce cell apoptosis.

Objective:To evaluate the clinical efficacy of the Magpie-bridge Microskin Grafting (hereinafter briefly referred to as Magpie-bridge surgery) in treating linear white scars (LWS).Methods:This study was a retrospective cohort study. From October 2022 to December 2023, 37 LWS patients were treated with the Magpie-bridge surgery at the Department of Plastic and Reconstructive Surgery of Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, including 9 males and 28 females, aged 25 (17, 36) years. All scars were <2 mm in width and 1–10 cm in length before surgery. Magpie-bridge surgery procedure: an electric punch was used to excise scar tissue at intervals to form wounds and harvest microskin grafts of the same size and thickness as the removed scar tissue from behind the ear or axillary apex. The microskin grafts were implanted at the wound sites and fixed with tension-reducing adhesive tape. The donor area wounds were treated with routine dressing changes. Twelve months after the first surgery, the efficacy was evaluated based on the degree of reduction in scar white area compared with that before the first surgery, and the treatment effectiveness rate was calculated. Before the first surgery and 12 months after the first surgery, the melanin scores of the normal skin area surrounding the scar and the scar area were evaluated using a skin imaging analysis system, and the difference between the two was calculated. Six of the aforementioned patients requested a second Magpie-bridge surgery in pursuit of better therapeutic effects. The white scar tissue left untreated during the first surgery (hereinafter referred to as untreated scar tissue) and the tissue from the site at 12 months post scar removal and microskin transplantation (hereinafter referred to as the recipient skin tissue at 12 months after the first surgery) were collected. The tissue structure, melanin quantity and distribution were examined by using hematoxylin eosin staining and Masson-Fontana staining, and the activity of tyrosinase positive melanocytes was observed by using immunofluorescence staining.Results:At 12 months after the first surgery, the results of efficacy evaluation showed that 24 cases were cured, 11 cases were improved, 1 case was ineffective, and 1 case was in a worsened condition, yielding a 94.6% (35/37) treatment effectiveness rate. The melanin score difference between the surrounding normal skin area of scar and the scar area was 0.45 (0.10, 1.65) at 12 months after the first surgery, which was significantly less than 2.50 (1.40, 5.96) before the first surgery ( Z=-5.02, P<0.05). Six patients had untreated scar tissue with flat epidermis and a flat junction between dermis and epidermis; the collagen fiber bundles in the dermis were thick and unidirectionally parallel; no skin appendages such as hair follicles were observed; the basal layer of the epidermis showed deposition of melanin particles, but no extensive depigmentation was observed. Compared with those of untreated scar tissue, the epidermal thickness increased, and epidermal protrusions appeared at the junction of dermis and epidermis of the recipient skin tissue at 12 months after the first surgery; hair follicles and sebaceous glands were visible, and collagen fibers in the dermis were arranged vertically and horizontally in an orderly manner. Melanin particles were deposited in the basal layer of the epidermis, and the melanin content per unit area of tissue was increased. Tyrosinase-positive melanocytes in untreated scar tissue and in the recipient skin tissue at 12 months after the first surgery were mainly located at the basal layer of the epidermis, with normal cell activity and no significant difference. Conclusions:The Magpie-bridge surgery can significantly improve the appearance of LWS in patients, with definite therapeutic effects and value for clinical promotion; the improvement of LWS appearance by Magpie-bridge surgery may be related to the increase of melanin content per unit area of tissue and the normalization of tissue structure.

Objective:To explore the efficacy of long intramedullary nails versus short intramedullary nails in the treatment of AO/OTA 31-A3 intertrochanteric fractures.Methods:A retrospective analysis was conducted on 60 patients with AO/OTA 31-A3 intertrochanteric femur fractures treated between March 2019 and August 2022. The patients were randomly divided into two groups (the long nail group and the short nail group). Thirty-four patients were treated with long intramedullary nails, including 16 males and 18 females, aged 68.41±17.84 years old (range 31-96 years). Twenty-six patients were treated with short intramedullary nails, including 13 males and 13 females, aged 72.23±13.97 years old (range 31-90 years). The causes of injury, fracture classification (AO/OTA classification), intraoperative blood loss, operation time, fracture healing time, imaging indexes (fracture reduction quality, postoperative neck trunk angle, and medial support), Harris score of the hip joint at the last follow-up, one-year mortality rates and complications were compared between the two groups.Results:The follow-up time was 24.26±6.67 months in the long nail group and 24.31±5.60 months in the short nail group, and the general information of the two groups were comparable. Between the long nail and short nail group, the intraoperative blood loss was 281.47±235.28 ml vs. 121.92±84.14 ml and the operation time was 110.44±24.63 min vs. 81.15±28.54 min with significant differences ( P<0.05). While the length of hospital stay was 12.35±4.81 d vs. 10.89±4.30 d, the good rate of fracture reduction was 55.9% vs. 61.53%, the fracture healing time was 120.44±16.43 d vs. 128.07±18.33 d, the presence rate of medial support was 67.6% vs. 79.4%, and the excellent rate of Harris score was 65.4% vs. 65.4% with no significant difference between the two groups ( P>0.05). One-year mortality rates was 5.3% vs. 7.1% and complications was 11.7% vs. 15.4% with no significant difference between the two groups ( P>0.05). Conclusion:Both long intramedullary nails and short intramedullary nails are effective in the treatment of AO/OTA 31-A3 intertrochanteric femur fractures. However, surgical time and intraoperative blood loss was less in the short nail group.

Objective:To evaluate the efficacy of perioperative analgesia with esketamine in the patients undergoing thoracoscopic surgery.Methods:A total of 90 patients of either sex, aged 18-64 yr, with body mass index of 18-30 kg/m 2, of American Society of Anesthesiologists Physical Status classification Ⅰ or Ⅱ, scheduled for elective thoracoscopic lobectomy under general anesthesia, were divided into 3 groups ( n=30 each) by a random number table method: control group (C group) and different doses of esketamine groups (S 1 group, S 2 group). Before induction of anesthesia, esketamine 0.1 and 0.2 mg/kg were intravenously injected in S 1 group and S 2 group, respectively, while esketamine was not given in group C. Anesthesia was routinely induced in all the three groups. During anesthesia maintenance, esketamine 0.1 and 0.2 mg·kg -1·h -1 were intravenously infused in group S 1 and group S 2, respectively, and the remaining drugs used for anesthesia maintenance were the same in the three groups. Patient-controlled intravenous analgesia (PCIA) was used after operation, and PCIA solution contained sufentanil 2 μg/kg in group C, and esketamine 1 mg/kg was mixed on the basis as previously described in S 1 and S 2 groups. Aminotriol ketorolac was given as rescue analgesia to maintain numeric rating scale score at rest ≤3. The total amount of propofol and remifentanil during operation, effective pressing times of PCIA in postoperative 0-24 h and >24-48 h periods, and requirement for rescue analgesia were recorded. The occurrence of adverse reactions such as respiratory depression, nausea and vomiting, dizziness and salivation, and emergence time were recorded after surgery. The serum interleukin-6 (IL-6) concentration was measured by enzyme-linked immunosorbent assay at 30 min before and after surgery, and the malondialdehyde (MDA) concentration in serum was measured by thiobarbituric acid colorimetric analysis. The postoperative recovery was assessed using the 50-item quality of recovery scale at 1 and 2 days after surgery. The development of chronic pain was followed up by telephone within 1-3 months after surgery. Results:Compared with group C, the intraoperative consumption of remifentanil, effective pressing times of PCIA in postoperative 0-24 h and >24-48 h periods, rate of rescue analgesia, and postoperative serum IL-6 concentration were significantly decreased, and the 50-item quality of recovery scale score was increased in S 1 and S 2 groups, and the postoperative serum MDA concentration was significantly decreased in group S 2 ( P<0.05). Compared with group S 1, the consumption of intraoperative remifentanil was significantly decreased ( P<0.05), and no significant change was found in postoperative serum IL-6 and MDA concentrations in group S 2 ( P>0.05). Compared with group S 2, the postoperative emergence time was significantly shortened in S 1 and C groups ( P<0.05). There was no statistically significant difference in the intraoperative consumption of propofol, incidence of adverse effects and incidence of chronic pain among the three groups ( P>0.05). Conclusions:Esketamine for perioperative analgesia (dose before anesthesia induction 0.1 mg/kg, dose for maintenance of anesthesia 0.1 mg·kg -1·h -1, dose for postoperative PCIA 1 mg/kg) can raise the quality of analgesia and improve the quality of early postoperative recovery in the patients undergoing thoracoscopic lobectomy.

Objective To investigate the efficacy of Jianpiwenyang Gel(SSWYG)for treating chronic diarrhea and explore its therapeutic mechanism.Methods Eighty patients with chronic diarrhea of spleen and stomach weakness type were randomized into two groups for interventions with lifestyle adjustment and treatment with bifid triple viable capsules(control group,n=40)or naval application with SSWYG(treatment group,n=40)for one week,after which symptoms of chronic diarrhea were evaluated.The Chinese medicine system pharmacology analysis platform(TCMSP),GeneCards,NCBI,OMIM database and GEO database(GSE14841)were used to obtain the active ingredients and target proteins of SSWYG and chronic diarrhea-related targets.The key targets were obtained by topological analysis for Gene Ontology(GO)and KEGG analyses.The affinity and binding characteristics of SSWYG for specific targets were verified by molecular docking using AutoDock software.Results In both groups,gastrointestinal symptom rating scale(GSRS),Bristol Scale and TCM syndrome scores significantly improved after the treatments(P<0.05),and better effects were observed in the treatment group(P<0.05).Sixty-eight targets of SSWYG in treating chronic diarrhea were obtained,and 33 most probable ones were screened out by topological analysis.GO and KEGG analyses identified several chronic diarrhea-related pathways including the TNF and IL-17 pathways.Molecular docking study showed good affinity of the core components of SSWYG for the key targets CASP3,JNK,IL1B,IL6,and AKT1.JUN and CASP3 had the lowest binding energy and the highest stable binding energy with multiple major active ingredients of SSWYG.Conclusion SSWYG can significantly improve clinical symptoms of chronic diarrhea possibly by regulating the TNF and IL-17 as well as other pathways via CASP3 and JUN,suggesting a complex therapeutic mechanism of SSWYG involving multiple ingredients and targets and coordinated regulation of multiple pathways.

Objective To explore the correlations between serum Mas-related G protein-coupled receptor X2 (MRGPRX2), interleukin (IL)-4, IL-5, IL-6, IL-13, IL-23 and IL-33 levels and chronic spontaneous urticaria (CSU). Methods The clinical characteristics and laboratory data from 55 patients with CSU and 21 healthy controls at Huashan Hospital, Fudan University from February 2021 to September 2023 were collected. The disease activity and severity of CSU patients were assessed. Serum level of MRGPRX2 was tested using enzyme-linked immunosorbent assay (ELISA), and levels of IL-4, IL-5, IL-6, IL-13, IL-23, and IL-33 were measured using Luminex multiplex assay in all subjects. Spearman correlation analysis was used to evaluate the correlations between biomarkers and other parameters in CSU patients, and logistic regression analysis was performed to identify factors influencing CSU. Results CSU patients exhibited significantly higher serum levels of MRGPRX2 (2.41[0, 11.51] ng/mL vs 0[0, 2.86] ng/mL, P＝0.015) and IL-23 (0.09[0.04, 0.56] pg/mL vs 0.05[0.03, 0.08] pg/mL, P＝0.033) than healthy controls. There was no difference in levels of other cytokines between the two groups. There was no difference in levels of MRGPRX2 and cytokines between severe and non-severe CSU patients. Correlation analysis showed that serum MRGPRX2 levels in CSU patients were positively correlated with IL-4 (r＝0.345, P＝0.010) and IL-6 (r＝0.395, P＝0.003) levels. Logistic regression analysis indicated that MRGPRX2≥0.055 ng/mL and IL-23≥0.135 pg/mL were independent risk factors for CSU (P＜0.05). Conclusions Serum levels of MRGPRX2 and IL-23 in CSU patients are elevated, which may be involved in the pathogenesis of CSU.

Objective:Using primary care chronic disease management as a case,it aims to explore an economic incentive model for integrated primary healthcare services based on value pricing.Additionally,practical needs and implementation recommendations are proposed.Methods:With the help of the health technology assessment framework,it proposes that integrated health services can be priced through service effectiveness and service utility,and develops an economic incentive model with value pricing at its core based on the patient-centered incentive model for innovative healthcare services,including financing,payment,appraisal,and distribution,and puts forward feasible suggestions in the light of the needs and actuality of primary integrated services in China.Conclusion and Recommendation:The value-based pricing model for integrated health services serves as a theoretical foundation for the transformation of primary healthcare service functions and the enhancement of service dynamics,aligning with China's value-oriented service procurement strategy.This research contributes to the academic discourse by providing localized insights and a scholarly tone,contributing to the advancement of knowledge in the field.

Objective:Using primary care chronic disease management as a case,it aims to explore an economic incentive model for integrated primary healthcare services based on value pricing.Additionally,practical needs and implementation recommendations are proposed.Methods:With the help of the health technology assessment framework,it proposes that integrated health services can be priced through service effectiveness and service utility,and develops an economic incentive model with value pricing at its core based on the patient-centered incentive model for innovative healthcare services,including financing,payment,appraisal,and distribution,and puts forward feasible suggestions in the light of the needs and actuality of primary integrated services in China.Conclusion and Recommendation:The value-based pricing model for integrated health services serves as a theoretical foundation for the transformation of primary healthcare service functions and the enhancement of service dynamics,aligning with China's value-oriented service procurement strategy.This research contributes to the academic discourse by providing localized insights and a scholarly tone,contributing to the advancement of knowledge in the field.