Objective To analyze the literature on acupuncture and moxibustion in promoting regeneration and repair published at home and abroad based on bibliometrics to identify the current status,hotspots,and trends of research in this field.Methods Chinese and English literature related to acupuncture and moxibustion in promoting regeneration and repair published in China Knowledge Network(CNKI)and Web of Science(WOS)Core Collection were visualized and analyzed using VOSviewer and Citespace software.Results Since the inception of the database to June 30,2024,1651 and 1437 documents were published in Chinese and English,respectively,showing a fluctuating upward trend over the past 20 years,with China being the country with the highest number of publications,Heilongjiang University of Traditional Chinese Medicine being the research institution with the highest number of publications in the CNKI database,and Beijing University of Traditional Chinese Medicine and Shanghai University of Traditional Chinese Medicine being the research institutions with the highest number of publications in the WOS database.The keyword analysis revealed that the research hotspot of acupuncture and moxibustion in promoting regeneration and repair focused on acupuncture therapies based on electroacupuncture,acupuncture and moxibustion,as well as research targets based on the brain,spinal cord and gastrointestinal mucosa,and the future research trend would be centered on the mechanism of action,focusing on the value of more acupuncture therapies in diseases such as spinal cord injury and ulcerative colitis.Conclusion In recent years,the research field of acupuncture and moxibustion in promoting regeneration and repair has developed rapidly,and various research teams have achieved rich results.The research hotspots have shown a diversified and deeper trend,and the application of acupuncture and moxibustion in promoting regeneration and repair has a broad prospect,and it is expected to provide more effective therapeutic means for the clinic.

Objective To analyze the literature on acupuncture and moxibustion in promoting regeneration and repair published at home and abroad based on bibliometrics to identify the current status,hotspots,and trends of research in this field.Methods Chinese and English literature related to acupuncture and moxibustion in promoting regeneration and repair published in China Knowledge Network(CNKI)and Web of Science(WOS)Core Collection were visualized and analyzed using VOSviewer and Citespace software.Results Since the inception of the database to June 30,2024,1651 and 1437 documents were published in Chinese and English,respectively,showing a fluctuating upward trend over the past 20 years,with China being the country with the highest number of publications,Heilongjiang University of Traditional Chinese Medicine being the research institution with the highest number of publications in the CNKI database,and Beijing University of Traditional Chinese Medicine and Shanghai University of Traditional Chinese Medicine being the research institutions with the highest number of publications in the WOS database.The keyword analysis revealed that the research hotspot of acupuncture and moxibustion in promoting regeneration and repair focused on acupuncture therapies based on electroacupuncture,acupuncture and moxibustion,as well as research targets based on the brain,spinal cord and gastrointestinal mucosa,and the future research trend would be centered on the mechanism of action,focusing on the value of more acupuncture therapies in diseases such as spinal cord injury and ulcerative colitis.Conclusion In recent years,the research field of acupuncture and moxibustion in promoting regeneration and repair has developed rapidly,and various research teams have achieved rich results.The research hotspots have shown a diversified and deeper trend,and the application of acupuncture and moxibustion in promoting regeneration and repair has a broad prospect,and it is expected to provide more effective therapeutic means for the clinic.

Objective:To analyze the genus, drug resistance/virulence and phylogenetic characteristics of Brucella strains isolated from brucellosis surveillance sentinels in Henan Province from 2013 to 2022, and provide baseline data for the surveillance, early warning and outbreak tracing of brucellosis. Methods:Blood samples were collected from patients with Brucella infection for strain isolation, culture and species identification, drug susceptibility test, whole genome sequencing, splicing and assembly, functional/virulence/resistance gene prediction analysis and phylogenetic tree drawing based on single nucleotide polymorphism (SNP). Results:In 36 brucellosis patients, the majority were men (86.11%, 31/36), young adults aged 18-50 (88.89%, 32/36) and farmers/herdsmen (72.22%, 26/36). A total of 36 strains of Brucella melitensis were isolated, and average 1 305 functional proteins of 21 categories were predicted by strain genome; all the strains carried four main virulence factors (pmm, VirB group, BtpA/BtpB, BvrS/BvrR). The drug sensitivity rate was 100.00% to six types of antibiotics including levofloxacin, rifampicin, doxycycline, streptomycin, tetracycline and gentamicin, they showed different resistances to three antibiotics including compound trimethoprim-sulfamethoxazole, ciprofloxacin and ampicillin. The strains carried four types of resistance genes and two clusters of resistance genes, with four combinations of genotypes, the resistance mechanisms included antibiotic degradation/modification enzymes, resistant nodular cell differentiation (RND) efflux pumps, 16S/23S ribosomal rRNA binding site mutations, etc. The number of SNP differed in the genomes of 36 Brucellamelitensis strains ranged from 0 to 454 and phylogenetic tree was divided into three major branches, with relative branch distances between 0.000 0 and 0.498 6 for each strain. Conclusions:Human Brucellamelitensis strains isolated from surveillance sentinels in Henan from 2013 to 2022 carried multiple virulence and antibiotic resistance genes and had different drug resistance phenotypes. Single nucleotide polymorphism analysis and phylogenetic tree analysis showed significant differences in phylogenetic relationships among different strains.

Objective:To systematically evaluate the clinical efficacy of fire needle therapy and conventional western medicine in the treatment of patients with acute herpes zoster.Methods:Randomized controlled trials (RCTs) of fire needle therapy in the treatment of acute herpes zoster were retrieved from China National Knowledge Internet (CNKI), Wanfang, Database of Chinese Sci Tech Periodicals (VIP Database), China Biomedical Literature Database (CBM), Medline, Embase, and Cochrane Library from the establishment of the databases to May 30, 2022. According to the inclusion and exclusion criteria, the included literature was screened, and Review Manager 5.3 was used for system evaluation and meta-analysis.Results:7 articles were included for analysis after screening, involving 452 patients. There were 226 cases in the treatment group and 226 cases in the control group. The results of meta-analysis showed that fire needle therapy was superior to conventional western medicine in the total effective rate [ RR=0.36, 95% CI (0.20, 0.65), P=0.000 7], lower incidence of PHN [ RR=0.25, 95% CI (0.09, 0.72), P=0.01], lower VAS score [ MD=-1.19, 95% CI (-2.14, -0.24), P=0.01], and shorter scab forming time [ MD=-2.03, 95% CI (-2.42, -1.64), P<0.001]. Conclusion:The curative effect of fire needle therapy is more prominent than that of conventional western medicine in the treatment of acute herpes zoster.

Objective To evaluate the diagnostic value of circulating tumor cells(CTCs) in prostate cancer (Pca) through studying the relationship between CTCs and Gleason scores and pathological TNM stage in Pca patients. Methods A total of 238 patients including 161 Pca patients as cancer group, 35 male patients with benign prostatic diseases as benign group and 42 male with non-prostate disease as control group, who were treated in our hospital from July 2016 to January 2018,were enrolled. Venous blood of every patient was collected and CTCs were enriched and identified by immunocytochemistry CD45 capturing leukocyte and fluorescence in situ hybridization with chromosome 8 (CEP8-FISH). Cells displaying CD45-/DAPI+/CEP8>2 were characterized as CTCs. One-way ANOVA was used to exam the correlations of the number of CTCs with Gleason scores and pathological TNM stage. Results CTCs ≥2 were detected in 74.53％(120/161) of Pca patients and 20.00％(7/35)of benign prostatic diseases patients and 7.14％(3/42)of control group (χ2=79.605,P<0.05). In group Gleason scores 6, the numbers of CTCs were 2.00 ± 2.42, the ratios of CTCs≥5 and tetraploid were 13.33％ (2/15)and 26.67％(4/15) respectively. In 7 scores group, the results were 3.14±2.68,17.72％(14/79) and 34.18％(27/79)respectively;In 8 scores group, the results were 3.57 ± 2.70, 33.33％(7/21)and 42.86％ (9/21)respectively; In 9 scores group, these three results were 4.65±4.41, 43.48％(20/46) and 45.65％(21/46)respectively. The numbers of CTCs in the≤pT2b (20), pT2c(27), pT3a(19), pT3b(16)and≥pT4(12) groups were 2.25±2.45, 3.56±2.79, 4.05±3.47, 4.69±2.12 and 5.17±3.21 respectively. The ratios of CTCs≥5 were 25.00％(5/20), 25.93％(7/27), 26.32％(5/19), 50.00％(8/16) and 58.33％ (7/12)respectively. The proportions of tetraploid were 20.00％(4/20), 25.93％ (7/27), 31.58％(6/19), 50.00％(8/16) and 58.33％(7/12) respectively. There were significant differences between CTC and Gleason scores (F=3.200, P<0.05)and pathological stage (F=2.673, P<0.05). The ratios of CTCs≥5 increased with the increase of Gleason scores (χ2=11.592, P<0.05). Conclusions The detection of CTCs could be used for the differential diagnosis of Pca and benign prostatic disease. There were notable correlations between the numbers of CTCs and Gleason scores and pathological stage in Pca patients, especially between CTCs≥5 and Gleason scores.

Objective To identify the astaxanthin, a major secondary metabolite produced by Rhodotorula sp, and to optimize the culture conditions for the production of astaxanthin. Methods The marine red yeast preservation strain was subjected to activation culture and fermentation culture. Single factor and response surface test was designed to optimize fermentation conditions. Secondary metabolites of marine red yeast were analyzed by HPLC, and secondary metabolites of marine red yeast were identified by standard samples. The antioxidant activity of marine red yeast astaxanthin was detected in the study. Results A strain of marine red yeast Rhodotorula sp isolated from a polar separation source was found to produce a variety of metabolites, the main metabolite of which was astaxanthin. Single factor experiments showed that the amount of added zinc sulfate was 100. 00 mg/L, and the highest yield of astaxanthin was 24. 10 mg/ml. The added amount of acetylsalicylic acid was 100. 00 mg/L, and the highest yield of astaxanthin was 25. 00 mg/ml. When fermentation time lasted for 5 days, the highest yield of astaxanthin was 25. 30 mg/mL. The surface response experiments indicated that the fermentation time was 6. 64 days, when the added amount of zinc sulfate was 113. 50 mg/L, and when the added amount of acetyl chloride was 111. 00 mg/L, the astaxanthin output in Rhodotorula sp. fermentation broth was 27. 00 mg/ml. The marine red yeast astaxanthin and other antioxidants have significant antioxidant activity. Conclusion Astaxanthin discovered from the polar separation source, the polar marine red yeast, Rhodotorula sp. is a main secondary metabolite. The use of zinc sulfate and acetylsalicylic acid to regulate the biosynthesis of astaxanthin could enrich the production of astaxanthin. The research results might provide an acess for large-scale production of astaxanthin.

Objective To identify the astaxanthin, a major secondary metabolite produced by Rhodotorula sp, and to optimize the culture conditions for the production of astaxanthin. Methods The marine red yeast preservation strain was subjected to activation culture and fermentation culture. Single factor and response surface test was designed to optimize fermentation conditions. Secondary metabolites of marine red yeast were analyzed by HPLC, and secondary metabolites of marine red yeast were identified by standard samples. The antioxidant activity of marine red yeast astaxanthin was detected in the study. Results A strain of marine red yeast Rhodotorula sp isolated from a polar separation source was found to produce a variety of metabolites, the main metabolite of which was astaxanthin. Single factor experiments showed that the amount of added zinc sulfate was 100. 00 mg/L, and the highest yield of astaxanthin was 24. 10 mg/ml. The added amount of acetylsalicylic acid was 100. 00 mg/L, and the highest yield of astaxanthin was 25. 00 mg/ml. When fermentation time lasted for 5 days, the highest yield of astaxanthin was 25. 30 mg/mL. The surface response experiments indicated that the fermentation time was 6. 64 days, when the added amount of zinc sulfate was 113. 50 mg/L, and when the added amount of acetyl chloride was 111. 00 mg/L, the astaxanthin output in Rhodotorula sp. fermentation broth was 27. 00 mg/ml. The marine red yeast astaxanthin and other antioxidants have significant antioxidant activity. Conclusion Astaxanthin discovered from the polar separation source, the polar marine red yeast, Rhodotorula sp. is a main secondary metabolite. The use of zinc sulfate and acetylsalicylic acid to regulate the biosynthesis of astaxanthin could enrich the production of astaxanthin. The research results might provide an acess for large-scale production of astaxanthin.

Objective To evaluate the effect of weak laser on treating hypertension,hyperglycemia,and hyperlipidemia.Methods A questionnaire-based survey was carrried out nationwide among the users of wrist-band weak laser irradiation.Changes in blood pressure,blood lipids,and blood glucose were analyzed before and after treatment.The influencial factors of antihypertensive effect of weak laser irradiation were analyzed by univariate and multivariate linear regression method.Results Questionnaire forms were returned from 5801 users,among whom 1335 patients with hypertension,180 patients with hyperglycemia,and 129 patients with hyperlipidemia entered the final analysis.After treatment,systolic blood pressure (SBP) (t =53.16,P ＜0.001),diastolic blood pressure (DBP) (t =34.17,P ＜ 0.001),triglycerides (TG) (t =7.87,P ＜0.001),total cholesterol (TC) (t =8.78,P ＜ 0.001),and fasting blood glucose (FBG) (t =11.64,P ＜0.001) were significantly decreased (compared with pre-treatment levels).Compared with the baseline,SBP,DBP,TG,TC and FBG fell by 23.3 mmHg (14.50％) (1 mmHg =0.133 kPa),10.6 mmHg (11.40％),0.86mmol/L (28.70％),1.11 mmol/L (18.00％),and 3.64 mmol/L (34.40％).The decrease of DBP in patients without insomnia was significantly higher than that in insomnia patients (F =9.54,P =0.00),and the decrease of DBP in non-drinkers was significantly higher than that in drinkers (F =5.08,P =0.02).The decrease of TG was significantly higher in males than in females (Z =2.48,P =0.01),in patients with mental stress than in those without stress (Z =-2.16,P =0.03),and in smokers than non-smokers (Z =-2.17,P =0.03).The median irradation time was 616 d (1-5479d).According to the irradiation time,the patients were further divided into four group:＜ 272d,272-698d,699-1435d,and ＞ 1437d.The SBP declined by (-22.25±16.22),(-21.59±15.24),(-24.96±16.82),and (-24.66±15.88) mmHg,respectively,in these four groups (F =3.55,P =0.01).Multivariate linear regression analysis showed that the influencial factors of SBP variation included gender (β =-0.08,P =0.0178) and irradiation time (β =-0.09,P =0.0016).The factors influencing the change of DBP were gender (β =-0.10,P =0.0039),insomnia (β =0.09,P =0.0015),and alcohol consumption (β =0.10,P =0.0033).Conclusion Long-time weak laser external irradiation has certain effectiveness in treating hypertension,hyperglycemia,and hyperlipidemia.

Objective To investigate the incidence of radioactivity uptake in thymus combined with serum thyroglobulin (Tg) increase in differentiated thyroid cancer (DTC) patients after high-dose 131I treatments,in order to discuss the mechanism of thymus iodine uptaking and Tg increasing.Methods Retrospective analysis of the laboratory examinations and 131Iwhole body scan (131I-WBS) images in 316 DTC patients were performed.The radioactivity uptake in thymus and the Tg level were observed.Results Among 316 patients (total 735 case-times),4 patients of 5 cases-times 131I-WBS showed radioactivity uptake in thymus,accounting for 0.68％ (5/735).All the radioactivity uptake in thymus were found by posttreatment 131I whole body scan (Rx-WBS) and after the second radioactive iodine treatment.For 1 of 4 patients,Rx-WBS still showed thymic uptake 131I after the third radioactive iodine treatment.The serum Tg increased in 3 patients (4 caestimes Rx-WBS) of radioactivity uptake in thymus with the Tg level before Rx-WBS was 13.80 μg/L,＞300.00 μg/L,16.40 μg/L,20.60μg/L,respectively.Conclusion In order to avoid the inappropriate administration of radioiodine therapy,thymic uptake should be identified carefully in DTC patients whose radioactivity uptake is only found at the upper mediastinal and combined with serum Tg increase.

Objective To investigate the effect of telerehabilitation on memory disorders. Methods From August, 2010 to April, 2015, 81 patients with memory disorders were randomized into control group (n=26), computer-assisted training group (n=33) and telerehabilita-tion training group (n=22). All the patients accepted medicine to facilitate the recovery of memory. Besides, the computer-assisted training group and the telerehabilitation training group accepted memory-based training programs with cognitive rehabilitation system locally or on network respectively, for six weeks. They were evaluated with Wechsler Memory Scale, Rivermead Behavioural Memory Test-2nd Edition and Rey Auditory Verbal Learning Test before and after training. Results Both computer-assisted and telerehabilitation training groups im-proved in all the assessment after training (t>4.059, P<0.001). There was no significant difference between them (P>0.05). There was no sig-nificant improvement in the control group after training (t<0.771, P>0.05). Conclusion Memory rehabilitation training can significantly im-prove memory abilities, similar with locally or telerehabilitation system.