This paper discusses the application of MRN combined with 3D printing in SNM in neurogenic bladder patients with special comorbidities. In this study, 21 cases of neurogenic bladder patients with special comorbidities involved, including 14 cases into the control group and 7 cases in the experimental group. Traditional X-ray guidance was used in the control group, while the MRN combined with 3D printing navigation template positioning was used in the experimental group. Compared with those in the control group, the X-ray fluoroscopy, number of punctures, puncture time, intraoperative adjustment and testing time were significantly decreased in the experimental group. The difference of implantable pulse generator implantation rates between the two groups was statistically significant.

The hinge structure, also known as hinge region or bend, is a special structure found in some antimicrobial peptides. Most studies on antimicrobial peptides focused on the standard secondary structure of α-helix and β-sheet, while the hinge structure and its functions were rarely studied. The hinge structure confers the antimicrobial peptides an improved structural flexibility, which may promote their disruptive effect on bacterial membrane or their binding efficiency to the intracellular targets, thus resulting in a higher antibacterial activity. Meanwhile, the hinge structure may reduce the structural rigidity, which may eliminate the cytotoxicity of antimicrobial peptides to eukaryotic cells. This article reviews the structural characteristics of the hinge structure, its effects on the biological activity of antimicrobial peptides and application in the molecular design, with the aim to provide a reference for the design and development of new antimicrobial peptides.
Anti-Bacterial Agents/pharmacology* ; Anti-Infective Agents/pharmacology* ; Antimicrobial Cationic Peptides/pharmacology* ; Pore Forming Cytotoxic Proteins ; Protein Structure, Secondary

Alpinia officinarum Hance of the Chinese traditional herb for the treatment of emesis,abdominal pain and diarrhea has been used to counteract gastric disease induced by indomethacin in rats without obvious side effects.However,the role of herb-drug interaction between indomethacin and A.officinarum based on pharmacokinetic,tissue distribution and excretion still remains unknown.In this study,an ultra-fast liquid-tandem mass spectrometry(UFLC-MS/MS)method was developed for simultaneous determina-tion of indomethacin and its three metabolites,O-desmethylindomethacin(ODI),deschlor-obenzoylindomethacin(NDI)and indomethacin acyl-β-D-glucuronide(IDAβG)by oral administration of indomethacin solution with and without the ethanolic extract of A.officinarum and applied to comparative pharmacokinetic,tissue distribution and excretion studies.Our results clarified that oral administration of A.officinarum produced significant alterations in the pharmacokinetic parameters of indomethacin.And the pharmacokinetic interaction between indomethacin and A.officinarum reduced the systemic exposure of indomethacin and increased its elimination.Tissue distribution results demonstrated that co-administration of A.Officinarum could not reduce the accumulation of indo-methacin in the target tissue of the stomach,but could accelerate the excretions of indomethacin and its three metabolites including ODI,NDI and IDAβG in the bile and feces of rats in the excretion study.Therefore,A.Officinarum might have a gastrointestinal protective effect through the interaction role with indomethacin based on the pharmacokinetics and excretion in rats.

Objective To investigate the function of JAK2-STAT3 signaling pathway in pancreatic injury and systematic inflammatory response in rats with acute necrotizing pancreatitis ( ANP) . Methods SD rats were randomly divided into the ANP group (n=48), ANP+JAK2 inhibitor Ruxolitinib group (ANP+R group, n=48), ANP+STAT3 inhibitot Stattic group (ANP+S group, n=48), ANP+Ruxolitinib+Stattic group (ANP+R+S group, n=48), and sham operation group (SO group, n=48). 5％ sodium taurocholate injection via retrograde pancreatobiliary duct was used to establish ANP model. Blood samples from abdominal aorta and pancreatic tissue were collected after 3 h, 6 h, 12 h and 18 h after modeling. Serum amylase (AMY) and tumor necrosis factor-α(TNF-α) and interleukin-4 (IL-4) were tested. JAK2 and STAT3 mRNA expression and protein expression of p-JAK2 and p-STAT3 in pancreas were examined by RT qPCR and western blot, respectively. Results AMY, TNF-α and IL-4 in plasma, and JAK2 mRNA, STAT3 mRNA, p-JAK2 protein and p-STAT3 protein at different time points in ANP group were all obviously higher than those in SO group(P<0. 05). Serum AMY, TNF-αand IL-4 in ANP+R group, ANP+S group and ANP+R+S group at different time points were lower than those in ANP group [12 h (5391 ± 1009),(6130 ± 1227),(4818 ± 992)U/L vs (8524 ± 1360) U/L;(147.25 ± 27.85),(156.25 ± 23.17),(127.87 ± 21.39) ng/L vs (187.58 ±20.09)ng/L;(45.89 ±16.95),(50.19 ±15.87),(38.87 ±14.03)ng/L vs (58.85 ±9.34)ng/L] . JAK2 mRNA and p-JAK2 protein,STAT3 mRNA and p-STAT3 protein in ANP+R group and ANP+R+S group at different time points were obviously lower than those in ANP group (12 h 0. 357 ± 0. 091 vs 0. 597 ± 0. 121,1. 115 ± 0. 203 vs 1. 217 ± 0. 213,0. 361 ± 0. 089 vs 0. 489 ± 0. 097,0. 965 ± 0. 189 vs 1. 128 ± 0. 217, 0. 362 ± 0. 092 vs 0. 597 ± 0. 121,1. 107 ± 0. 212 vs 1. 217 ± 0. 213,0. 297 ± 0. 087 vs 0. 489 ± 0. 097,0. 713 ± 0. 184 vs 1. 128 ± 0. 217). STAT3 mRNA and p-STAT3 protein in ANP+S group were obviously lower than those in ANP group(0. 319 ± 0. 107 vs 0. 489 ± 0. 097,0. 849 ± 0. 177 vs 1. 128 ± 0. 217), and the difference was statistically different (P<0.05). Conclusions The activation of JAK2-STAT3 signaling pathway in pancreas may play a key role in the pathogenesis of systematic inflammatory response in ANP.

Objective To understand the health literacy status,influencing factors and demand carrying out the health education based on the mobile terminal among college students to provide a new idea for formulating and implementing the health literacy promotion plan for university students.Methods The multi-stage stratified cluster random sampling method was adopted to extract 1 949 students from partial universities in Nanjing City and conduct the investigation by adopting the Rapid Estimate System of Health Literacy and Health Education Demand Based on the Mobile Terminal Questionnaire of College Students.The influence factors were analyzed by using the univariate analysis and ordinal multi-classification Logistic regression analy sis.Results The university students with high,marginal and low health literacy level accounted for 31.3％,48.9％ and 19.8％ respectively.The proportions of the students with high health literacy in the four dimensions,including health knowledge,health behaviors,health skill and health status,were 57.9％,18.3％,61.0％ and 44.5％ respectively.The ordinal multi-classification Logistic regression analysis showed that the major influence factors affecting the health literacy of university students were gender,education background of mothers,majors,grades,electively taking the health education course.81.2 ％ of university students wanted to or urgently wanted to understand the health literacy knowledge.The main route getting the health literacy knowledge by the university students was network(83.8％).91.8％ of students were willing to learn the health literacy knowledge through mobile terminal.87.1％ of students believed that the WeChat was suitable to learn the health knowledge,81.3％ of students hoped to use the WeChat platform for learning the health knowledge,73.8％ of students hoped the Wechat to push the health knowledge with the modes of words combined with images and video.It was recommended that the health knowledge pushing for 1-3 times per week was suitable.Conclusion The health literacy of university students is at the marginal level.The health behaviors literacy is most lacking and the demand for the health education based on the mobile terminal is stronger.The health literacy intervention of university students should sufficiently apply the new media platform superiority and the emphasis should be placed on the populations of males,low education background mothers,non-medicine major,low grade and without electively taking the health education course.

Objective To explore the in vivo/in vitro MR imaging effect of Angiopep2 modified glioma targeted block copolymer micelles(ANPs/CPT). Methods Angiopep2 modified DOTA-Gd conjugated polymeric micelles based on beta-cyclodextrin(β-CD) were successfully synthesized.Water proton longitudinal relaxation rate (1/T1) were calculated to compare the r1of DOTA-Gd and ANPs/CPT. Different concentration of DOTA-Gd, NP/CPT, ANP/CPT were co-cultured with C6 cells, the intracellular content of Gd3+was analyzed by ICP-AES;The signal intensity(SI) and contrast to noise ratio(CNR) were measured by 1.5 T MR;The CNR of ANPs/CPT was evaluated on different organs of normal rats. The enhancement feature of ANP/CPT in glioma as well as T/N and CNR between tumor and normal brain tissue were also investigated.Several comparisons were made between the 3 groups by one-way ANOVA,and the difference were significant when P<0.05.Results The r1of ANPs/CPT(13.900 s-1·mmol/L-1)was 3 times as much as DOTA-Gd(4.927 s-1·mmol/L-1).C6 cells uptake different concentrations of Gd3 +had significant difference among groups(F=362.502,1636.136,386.880,918.173, P<0.001).The difference of SI and CNR among groups were significant(F=55.240,155.419,P<0.001).The CNR of the brain tissue had significant difference with other organs at all times after tail vein injection of ANPs/CPT(F=9.417,21.808, 21.383,107.318,178.762,P<0.01).The ANPs/CPT showed excellent brain-targeting efficiency and theT/N ratio(30 min after),CNR in different time point had significant difference among groups (F=5.349,6.594,24.078,18.508 and 6.840,6.780,5.895,12.620,37.139,368.893, P<0.05).Conclusions ANPs/CPT shows nice agnetic relaxation performance,extended blood circulation duration,excellent brain-targeting efficieney, which will render the theranosticnano-carriers with important reference for the construction of new generation multifunctional nanomedicine.

Objective To evaluate the diagnostic capabilities of T2 WI features in peripheral zone prostate cancer.Methods The characteristics of T2 WI in 56 cases of peripheral zone prostate cancer,75 cases of benign prostatic hyperplasia and 7 cases of prostatitis confirmed by pathology were analyzed retrospectively.Eight indexes were initially screened byχ2 test,then indexes with significant difference were entered into multivariate Logistic regression analysis.Results The indexes showing statistical differences between prostate cancer and benign prostatic disorders were as following:lesion shape,signal intensity,signal uniformity,boundary of peripheral zone and transition zone,the volume of peripheral zone and prostatic capsule on T2 WI (P<0.05).Margin of lesion and capsule between two groups were not statistical differences (P>0.05).By multivariate Logistic analysis,the statistically significant difference were found in lesion shape, the volume of peripheral zone,prostatic capsule and boundary of peripheral zone and transition zone.Conclusion The lesion shape, volume of peripheral zone and prostatic capsule on T2 WI are independent risk factors for peripheral zone prostate cancer.

OBJECTIVE:To observe analgesic effect and safety of Shixin buccal tablets on pericoronitis of wisdom tooth. METHODS:120 patients with pericoronitis of wisdom tooth were randomly divided into control group and observation group with 60 cases in each group. Control group received periodontal pocket irrigation combined with metronidazole;observation group was additionally given Shixin buccal tablets on the basis of control group,1 tablet/time,4 times/d. Clinical efficacy,medication compli-ance,pain score and ADR were compared between 2 groups. RESULTS:The effective rate of observation group(95.00%)was sig-nificantly higher than that of control group(73.33%),with statistical significance(P<0.05);there was no statistical significance in medication compliance between 2 groups (P>0.05);the pain score of observation group was significantly lower than that of control group,with statistically significant difference(P<0.05). No serious ADR was found in 2 groups. CONCLUSIONS:Shixin buccal tablets significantly alleviate the pain caused by pericoronitis of wisdom tooth.

Objective To develop a reversed phase high performance liquid chromatography ( RP￣HPLC) method determining the related substances and levidipine. Methods The Welchrom C18 column (250 mm×4.6 mm,5 μm) was used with Octadecylsilane bonded silica as a filler.The mobile phase consisted of methanol￣ethanol￣water (40:20:40),at the flow rate of 1 mL.min-1 in an isocratic elution,the temperature was at 45 ℃ , and the detective wavelength was 240 nm. Results Levidipine could be separated from all impurities and intermediates within the concentration range from151 to 604 ng.mL-1 , which had a satisfied linear relationship (r= 0.999 9) with a regression equation of Y= 0.049 9X+0.597 9.The LOQ of detection was 31.9 ng.mL-1 . Conclusion The developed method is specific,sensitive,easy and fast to operate,which is suitable for detecting levidipine and its related impurities.

OBJECTIVE:To observe clinical efficacy of recurrent oral ulceration with combination of Zhibai dihuang pills and Compound danshen tablets. METHODS:110 patients with recurrent oral ulceration were selected and divided into control group and tri-al group with 55 cases in each group. Control group individually received vitamin C 0.2 g/time+vitamin B2 10 mg/time+levamisole 45 mg/time,three times a day,Cetylpyridinium chloride collutory,15 ml/time,for 1 min,3 times a day. Trial group received Zhibai di-huang pills orally,8 pills/time,Compound danshen tablets,3 tablets/time,3 times a day. A treatment course lasted for 10 days. Both groups received 2 courses of treatment. Therapeutic efficacy,VAS and relapse were observed and compared between 2 groups. RE-SULTS:The cure rate(80.00%)and total effective rate(94.55%)of trial group were higher than those of control group(47.27%, 74.55%),with statistical significance(P<0.05). The VAS score of trial group were significantly lower than that of control group after 3 and 7 days of treatment,with statistical significance(P<0.05). After 2,6,10 and 12 months of treatment,the recurrence rates of tri-al group were 0,7.27%,9.09% and 16.36%,which were all significantly lower than those of control group(18.18%,34.55%, 47.27%,56.36%),with statistical significance(P<0.05). CONCLUSIONS:Combination of Zhibai dihuang pills and Compound danshen tablets has definite effect on recurrent oral ulcer,showing high cure rate and not easily recur.