Acute kidney injury (AKI) is a critical clinical condition characterized by rapid renal function decline, with high morbidity, mortality, and healthcare costs. Traditional Chinese medicine (TCM) has shown potential effects on mitigating oxidative stress and programmed cell death in AKI models. Scutellaria barbata D. Don (SB) and Scleromitrion diffusum (Willd.) R. J. Wang (SD), a classic TCM herbal pair exhibited anti-inflammatory and antioxidant activities. Using advanced chromatographic separation technology, we enriched the effective fractions of water extracts from SB-SD, obtaining self-assembled herbal nanoparticles (SB and SD nanoparticles, SSNPs) rich in flavonoids and terpenoids. These SSNPs demonstrated robust antioxidant properties in vitro and mitigated AKI progression in vivo by activating the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway. Oral administration of SSNPs in mice resulted in absorption into the bloodstream, formation of a protein corona, reduced macrophage phagocytosis, and enhanced bioavailability and renal targeting. Furthermore, we investigated the self-assembly principle of SSNPs using representative flavonoids and terpenoids. Kinetic studies and in situ transmission electron microscopy (in situ TEM) revealed that these compounds self-assemble via supramolecular forces like hydrogen bonding and π-π interactions, forming stable nanostructures. This study elucidates the renoprotective effects and mechanisms of SB and SD, and provides a novel approach for the development of TCM-based nanomedicines, highlighting the potential of nano-TCM in AKI treatment.

Discipline construction is an important part of the high-quality development of public hospitals.Under the current single multi-district development trend of public hospitals,it is necessary to layout disciplines in a scientific and systematic way to realize the coordinated development of each district at the geographical and functional levels.From the perspective of top-level design of multi-district discipline layout and practical exploration of Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine,it is proposed to clarify the functional orientation of the hospital districts from the perspective of the concept of running the hospital,national tasks and social needs.To achieve the high-quality development of the discipline and the continuous upgrading and innovation of public hospitals,it is also suggested to clarify the current situation of the discipline and determine the classified development direction by constructing a systematic disciplinary evaluation model.The transformation of scientific and technological achievements is a breakthrough to cultivate new momentum for the development of the hospital district,so as to realise the high-quality development of disciplines and the continuous upgrading and innovation of the functions of the hospital district.

Discipline construction is an important part of the high-quality development of public hospitals.Under the current single multi-district development trend of public hospitals,it is necessary to layout disciplines in a scientific and systematic way to realize the coordinated development of each district at the geographical and functional levels.From the perspective of top-level design of multi-district discipline layout and practical exploration of Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine,it is proposed to clarify the functional orientation of the hospital districts from the perspective of the concept of running the hospital,national tasks and social needs.To achieve the high-quality development of the discipline and the continuous upgrading and innovation of public hospitals,it is also suggested to clarify the current situation of the discipline and determine the classified development direction by constructing a systematic disciplinary evaluation model.The transformation of scientific and technological achievements is a breakthrough to cultivate new momentum for the development of the hospital district,so as to realise the high-quality development of disciplines and the continuous upgrading and innovation of the functions of the hospital district.

Discipline construction is an important part of the high-quality development of public hospitals.Under the current single multi-district development trend of public hospitals,it is necessary to layout disciplines in a scientific and systematic way to realize the coordinated development of each district at the geographical and functional levels.From the perspective of top-level design of multi-district discipline layout and practical exploration of Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine,it is proposed to clarify the functional orientation of the hospital districts from the perspective of the concept of running the hospital,national tasks and social needs.To achieve the high-quality development of the discipline and the continuous upgrading and innovation of public hospitals,it is also suggested to clarify the current situation of the discipline and determine the classified development direction by constructing a systematic disciplinary evaluation model.The transformation of scientific and technological achievements is a breakthrough to cultivate new momentum for the development of the hospital district,so as to realise the high-quality development of disciplines and the continuous upgrading and innovation of the functions of the hospital district.

Discipline construction is an important part of the high-quality development of public hospitals.Under the current single multi-district development trend of public hospitals,it is necessary to layout disciplines in a scientific and systematic way to realize the coordinated development of each district at the geographical and functional levels.From the perspective of top-level design of multi-district discipline layout and practical exploration of Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine,it is proposed to clarify the functional orientation of the hospital districts from the perspective of the concept of running the hospital,national tasks and social needs.To achieve the high-quality development of the discipline and the continuous upgrading and innovation of public hospitals,it is also suggested to clarify the current situation of the discipline and determine the classified development direction by constructing a systematic disciplinary evaluation model.The transformation of scientific and technological achievements is a breakthrough to cultivate new momentum for the development of the hospital district,so as to realise the high-quality development of disciplines and the continuous upgrading and innovation of the functions of the hospital district.

Discipline construction is an important part of the high-quality development of public hospitals.Under the current single multi-district development trend of public hospitals,it is necessary to layout disciplines in a scientific and systematic way to realize the coordinated development of each district at the geographical and functional levels.From the perspective of top-level design of multi-district discipline layout and practical exploration of Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine,it is proposed to clarify the functional orientation of the hospital districts from the perspective of the concept of running the hospital,national tasks and social needs.To achieve the high-quality development of the discipline and the continuous upgrading and innovation of public hospitals,it is also suggested to clarify the current situation of the discipline and determine the classified development direction by constructing a systematic disciplinary evaluation model.The transformation of scientific and technological achievements is a breakthrough to cultivate new momentum for the development of the hospital district,so as to realise the high-quality development of disciplines and the continuous upgrading and innovation of the functions of the hospital district.

Discipline construction is an important part of the high-quality development of public hospitals.Under the current single multi-district development trend of public hospitals,it is necessary to layout disciplines in a scientific and systematic way to realize the coordinated development of each district at the geographical and functional levels.From the perspective of top-level design of multi-district discipline layout and practical exploration of Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine,it is proposed to clarify the functional orientation of the hospital districts from the perspective of the concept of running the hospital,national tasks and social needs.To achieve the high-quality development of the discipline and the continuous upgrading and innovation of public hospitals,it is also suggested to clarify the current situation of the discipline and determine the classified development direction by constructing a systematic disciplinary evaluation model.The transformation of scientific and technological achievements is a breakthrough to cultivate new momentum for the development of the hospital district,so as to realise the high-quality development of disciplines and the continuous upgrading and innovation of the functions of the hospital district.

Discipline construction is an important part of the high-quality development of public hospitals.Under the current single multi-district development trend of public hospitals,it is necessary to layout disciplines in a scientific and systematic way to realize the coordinated development of each district at the geographical and functional levels.From the perspective of top-level design of multi-district discipline layout and practical exploration of Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine,it is proposed to clarify the functional orientation of the hospital districts from the perspective of the concept of running the hospital,national tasks and social needs.To achieve the high-quality development of the discipline and the continuous upgrading and innovation of public hospitals,it is also suggested to clarify the current situation of the discipline and determine the classified development direction by constructing a systematic disciplinary evaluation model.The transformation of scientific and technological achievements is a breakthrough to cultivate new momentum for the development of the hospital district,so as to realise the high-quality development of disciplines and the continuous upgrading and innovation of the functions of the hospital district.

Discipline construction is an important part of the high-quality development of public hospitals.Under the current single multi-district development trend of public hospitals,it is necessary to layout disciplines in a scientific and systematic way to realize the coordinated development of each district at the geographical and functional levels.From the perspective of top-level design of multi-district discipline layout and practical exploration of Ruijin Hospital affiliated to Shanghai Jiaotong University School of Medicine,it is proposed to clarify the functional orientation of the hospital districts from the perspective of the concept of running the hospital,national tasks and social needs.To achieve the high-quality development of the discipline and the continuous upgrading and innovation of public hospitals,it is also suggested to clarify the current situation of the discipline and determine the classified development direction by constructing a systematic disciplinary evaluation model.The transformation of scientific and technological achievements is a breakthrough to cultivate new momentum for the development of the hospital district,so as to realise the high-quality development of disciplines and the continuous upgrading and innovation of the functions of the hospital district.

Background Natural pyrethrins have long been widely used in the fields of environmental and household hygiene. Studies have reported that natural pyrethrins have potential liver toxicity, but their specific mechanisms are still unclear yet. Objective To explore the effect of natural pyrethrins on DNA damage in human liver cells. Methods This study used human liver cell QSG7701 as an in vitro testing model. After exposure to DMSO and a series of concentrations of natural pyrethrins (5, 10, 20, and 40 μg·mL⁻¹) for 6 and 24 h, reactive oxygen species (ROS) was detected by fluorescence microscopy using a fluorescence probe, thiobarbituric acid reactive substance (TBARS) by colorimetric method using a microplate reader, DNA damage by comet assay through observing DNA fragment migration under microscope, and phospho H2AX (γH2AX) and 8-oxoguanine (8-oxoG) by immunofluorescence assay using a laser confocal microscope. Results As the exposure concentration of natural pyrethrins increased, the fluorescence intensity of ROS significantly increased in a concentration-dependent manner. The differences in ROS between the 10 μg·mL⁻¹ and above groups and the control group were statistically significant (P<0.01), and the ROS levels in the 20 μg·mL⁻¹ and 40 μg·mL⁻¹ treatment groups were 2.17 and 3.05 times higher than that in the control group respectively. The TBARS level increased in a concentration-dependent manner in natural pyrethrins treated cells (P<0.01), and the levels in the 20 μg·mL⁻¹ and 40 μg·mL⁻¹ treatment groups were 2.46 and 3.01 times higher than that in the control group respectively. The results of comet assay showed trailing formation of cellular DNA in each dose group; as the exposure concentration of natural pyrethrins increased, indicators such as tail DNA content (TDNA%), tail length (TL), tail moment (TM), and Olive tail moment (OTM) increased in a concentration-dependent manner. Compared with the control group, the differences in the indicators between the 20 μg·mL⁻¹ and above groups and the control group were statistically significant (P<0.01), especially in the 40 μg·mL⁻¹ treatment groups, where TDNA%, TL, TM, and OTM were (46.92 ± 3.52) %, (64.67± 4.16) μm, 30.96 ± 2.94, and 22.64 ± 3.89, respectively. The cellular immunofluorescence results showed that natural pyrethrins induced the formation of γH2AX and 8-oxoG, the fluorescence intensities of γH2AX and 8-oxoG increased in a concentration-dependent manner, and the differences between the 10 μg·mL⁻¹ and above groups and the control group were statistically significant (P<0.01). Conclusion Natural pyrethrins could induce DNA damage in human liver cells, and ROS-mediated oxidative stress may play an important role in its liver cell genotoxicity.