[Purpose]To analyze the results of cervical cancer screening among married women of 25～64 years old in Shihezi City from 2021 to 2023,and to explore the risk factors of cervical le-sions.[Methods]The results of cervical cancer screening among married women aged 25～64 years old in Shihezi City from 2021 to 2023 were retrospectively analyzed.The detection rates of common gynecological diseases,cervical cancer and precancerous lesions and human papillo-mavirus(HPV)infection by cervical cytology,colposcopy and cervical biopsy were analyzed.[Re-sults]The HPV infection(mainly single infection)rate was 10.74％.The common gynecological diseases were vaginitis,cervicitis and uterine leiomyoma.The detection rate of cervical cancer was 19.59/105,and the detection rate of cervical precancerous lesions was 308.52/105.Rural resi-dents,HPV 16/18 infection,genital warts were the risk factors for cervical lesions.[Conclusion]Female cervical lesions are significantly correlated with HPV 16/18 infection,genital condyloma acuminatum,particularly for rural residents,and preventive care,early screening and interven-tion should be strengthened for these populations.

Liver cirrhosis is the terminal stage of chronic liver disease. Minimizing complications of liver cirrhosis and the occurrence of liver cancer has become an important goal for liver cirrhosis treatment. The primary etiology leading to liver cirrhosis is chronic hepatitis B infection. "Dual-anti therapy" refers to the combination of anti-viral and anti-fibrosis treatment, which is the characteristic and advantage of the integration of Chinese and Western medicine. The article elaborates on the concept and strategies of "dual antibody therapy" based on evidence-based medicine and introduces the research progress in terms of representative anti-fibrotic Chinese patent medicines for reversing liver cirrhosis, reducing the occurrence of hepatocellular carcinoma, and loweri ng portal hypertension and its complications and others, revealing that dual antibody therapy can clinically facilitate dual reduction" (reducing both liver cancer and complications of cirrhosis). Additionally, it analyzes and summarizes the mechanism of action of anti-fibrotic Chinese patent medicines and prospects of a new model of liver cirrhosis treatment combining Chinese and Western medicine, thereby providing novel ideas for the prevention and treatment of clinical liver diseases.

With reference to the Information and Documentation-Resource Description (GB/T 3792-2021) and Bibliographical Description for Ancient Chinese Books (GB/T 3792.7-2008) and other cataloging standards and rules, drawing on the practical experience of cataloging ancient TCM books, Bibliographical Cataloging for Ancient TCM Books was formulated. This standard specifies the entry items and their order of ancient TCM books, cataloging identifier, cataloging text, cataloging information source, and cataloging item details. The standard can provide standardized and unified guiding principles and methods for the work of ancient TCM books, and promote the sharing and utilization of ancient TCM books.

Objective To investigate the impact of Mycoplasma infection(MP)on the reproductive tract microbiota of patients with endometritis.Methods A retrospective analysis was conducted on the clinical data of patients with endometritis who was visited in the First People's Hospital of Shuangliu District from April 2023 to April 2024.Based on inclusion and exclusion criteria,a total of 60 subjects were ultimately included in each of the MP-positive and MP-negative groups.Univariate analysis was used to compare the differences in inflammation,immunity,endometrial morphology,and disease severity between the two groups.The Illumina Nova sequencing platform was used to sequence and analyze the reproductive tract secretions of 120 subjects.The detected microbial flora was subdivided into different taxonomic levels including kingdom,phylum,class,order,family,genus and species.Mothur software was employed to calculate the Shannon diversity index and Simpson diversity index to evaluate species richness.The Wilcoxon rank-sum test was used to compare the abundance of colonies between the two groups.Using R software(v4.0.1),evaluated the differences in gene ontology(GO)annotations and metabolic pathways of Kyoto encyclopedia of genes and genomes(KEGG)pathways between different groups.Results Compared with the MP-negative group,the MP-positive group exhibited statistically significant differences in levels of inflammatory and immune indicators,as well as in the proportions of congestion and edema,atypical blood vessels,varying thicknesses,and severe disease severity(t/χ2=3.043～26.830,all P<0.05).The reproductive tract flora of the two groups was divided into four phyla:Proteobacteria,Actinobacteria,Firmicutes and Bacteroidetes.The abundance of Proteobacteria(47.95%vs 33.32%),Bacteroidetes(9.48%vs 3.41%)and Actinobacteria(4.11%vs 2.04%)increased,while the abundance of Firmicutes(38.46%vs 61.23%)decreased in the MP-positive group compared to the MP-negative group,and the differences were statistically significant(χ2=10.126～12.745,all P<0.05).A comparison of the α-diversity analysis results of the reproductive tract microbiota between the two patient groups revealed that the Shannon index and Simpson index in the MP-positive group were 4.81±0.14 and 1.68±0.07,respectively,while those in the MP-negative group were 4.07±0.18 and 1.42±0.04,respectively.The Shannon index and Simpson index in the MP-positive group were both significantly higher than those in the MP-negative group,and the differences were statistically significant(t=9.342,9.583,all P<0.001).Compared with the MP-negative group,the abundance of Streptococcus[proportion difference(PD):5.26,95%CI:2.63～6.27],Escherichia(PD:2.71,95%CI:1.34～4.58),Staphylococcus(PD:4.00,95%CI:2.06～6.14),Enterococcus(PD:4.10,95%CI:1.02～4.58),Mycoplasma(PD:6.05,95%CI:3.08～8.79),Pseudomonas(PD:2.97,95%CI:1.28～4.06)and Gardnerella(PD:6.07,95%CI:2.59～8.75)increased in the MP-positive group,while the abundance of Lactobacillus(PD:-19.22,95%CI:-21.97～-10.39)and Bifidobacterium(PD:-16.91,95%CI:-24.06～-10.57,)decreased,and the differences were statistically significant(t=3.857～11.306,all P<0.05).Analysis of metabolic pathways in the two patient groups revealed that,within the MP-positive chronic endometritis(CE)cohort,functional gene categories with significantly increased relative enrichment included ATP-binding function,DNA repair processes,inosine monophosphate biosynthetic processes,fatty acid biosynthetic processes,arginine biosynthetic processes,and peptidase activity.Analysis of signaling pathways in both patient groups found that ABC transporter complexes,aminoacyl-tRNA biosynthesis,unsaturated fatty acid biosynthesis,phenylalanine metabolism,tyrosine metabolism,tryptophan metabolism,and insulin signaling pathways were notably active in MP-positive CE patients.Conclusion Compared to MP-negative CE patients,MP-positive patients exhibited more pronounced inflammatory and immune responses,as well as a higher proportion of endometrial morphological abnormalities and severe disease severity.The reproductive tract microbiota diversity increased in MP-positive CE patients,with Streptococcus becoming the dominant genus and a decreasing trend in the relative abundance of Lactobacillus.

Liver cirrhosis is the terminal stage of chronic liver disease. Minimizing complications of liver cirrhosis and the occurrence of liver cancer has become an important goal for liver cirrhosis treatment. The primary etiology leading to liver cirrhosis is chronic hepatitis B infection. "Dual-anti therapy" refers to the combination of anti-viral and anti-fibrosis treatment, which is the characteristic and advantage of the integration of Chinese and Western medicine. The article elaborates on the concept and strategies of "dual antibody therapy" based on evidence-based medicine and introduces the research progress in terms of representative anti-fibrotic Chinese patent medicines for reversing liver cirrhosis, reducing the occurrence of hepatocellular carcinoma, and loweri ng portal hypertension and its complications and others, revealing that dual antibody therapy can clinically facilitate dual reduction" (reducing both liver cancer and complications of cirrhosis). Additionally, it analyzes and summarizes the mechanism of action of anti-fibrotic Chinese patent medicines and prospects of a new model of liver cirrhosis treatment combining Chinese and Western medicine, thereby providing novel ideas for the prevention and treatment of clinical liver diseases.

Objective To investigate the impact of Mycoplasma infection(MP)on the reproductive tract microbiota of patients with endometritis.Methods A retrospective analysis was conducted on the clinical data of patients with endometritis who was visited in the First People's Hospital of Shuangliu District from April 2023 to April 2024.Based on inclusion and exclusion criteria,a total of 60 subjects were ultimately included in each of the MP-positive and MP-negative groups.Univariate analysis was used to compare the differences in inflammation,immunity,endometrial morphology,and disease severity between the two groups.The Illumina Nova sequencing platform was used to sequence and analyze the reproductive tract secretions of 120 subjects.The detected microbial flora was subdivided into different taxonomic levels including kingdom,phylum,class,order,family,genus and species.Mothur software was employed to calculate the Shannon diversity index and Simpson diversity index to evaluate species richness.The Wilcoxon rank-sum test was used to compare the abundance of colonies between the two groups.Using R software(v4.0.1),evaluated the differences in gene ontology(GO)annotations and metabolic pathways of Kyoto encyclopedia of genes and genomes(KEGG)pathways between different groups.Results Compared with the MP-negative group,the MP-positive group exhibited statistically significant differences in levels of inflammatory and immune indicators,as well as in the proportions of congestion and edema,atypical blood vessels,varying thicknesses,and severe disease severity(t/χ2=3.043～26.830,all P<0.05).The reproductive tract flora of the two groups was divided into four phyla:Proteobacteria,Actinobacteria,Firmicutes and Bacteroidetes.The abundance of Proteobacteria(47.95%vs 33.32%),Bacteroidetes(9.48%vs 3.41%)and Actinobacteria(4.11%vs 2.04%)increased,while the abundance of Firmicutes(38.46%vs 61.23%)decreased in the MP-positive group compared to the MP-negative group,and the differences were statistically significant(χ2=10.126～12.745,all P<0.05).A comparison of the α-diversity analysis results of the reproductive tract microbiota between the two patient groups revealed that the Shannon index and Simpson index in the MP-positive group were 4.81±0.14 and 1.68±0.07,respectively,while those in the MP-negative group were 4.07±0.18 and 1.42±0.04,respectively.The Shannon index and Simpson index in the MP-positive group were both significantly higher than those in the MP-negative group,and the differences were statistically significant(t=9.342,9.583,all P<0.001).Compared with the MP-negative group,the abundance of Streptococcus[proportion difference(PD):5.26,95%CI:2.63～6.27],Escherichia(PD:2.71,95%CI:1.34～4.58),Staphylococcus(PD:4.00,95%CI:2.06～6.14),Enterococcus(PD:4.10,95%CI:1.02～4.58),Mycoplasma(PD:6.05,95%CI:3.08～8.79),Pseudomonas(PD:2.97,95%CI:1.28～4.06)and Gardnerella(PD:6.07,95%CI:2.59～8.75)increased in the MP-positive group,while the abundance of Lactobacillus(PD:-19.22,95%CI:-21.97～-10.39)and Bifidobacterium(PD:-16.91,95%CI:-24.06～-10.57,)decreased,and the differences were statistically significant(t=3.857～11.306,all P<0.05).Analysis of metabolic pathways in the two patient groups revealed that,within the MP-positive chronic endometritis(CE)cohort,functional gene categories with significantly increased relative enrichment included ATP-binding function,DNA repair processes,inosine monophosphate biosynthetic processes,fatty acid biosynthetic processes,arginine biosynthetic processes,and peptidase activity.Analysis of signaling pathways in both patient groups found that ABC transporter complexes,aminoacyl-tRNA biosynthesis,unsaturated fatty acid biosynthesis,phenylalanine metabolism,tyrosine metabolism,tryptophan metabolism,and insulin signaling pathways were notably active in MP-positive CE patients.Conclusion Compared to MP-negative CE patients,MP-positive patients exhibited more pronounced inflammatory and immune responses,as well as a higher proportion of endometrial morphological abnormalities and severe disease severity.The reproductive tract microbiota diversity increased in MP-positive CE patients,with Streptococcus becoming the dominant genus and a decreasing trend in the relative abundance of Lactobacillus.

[Purpose]To analyze the results of cervical cancer screening among married women of 25～64 years old in Shihezi City from 2021 to 2023,and to explore the risk factors of cervical le-sions.[Methods]The results of cervical cancer screening among married women aged 25～64 years old in Shihezi City from 2021 to 2023 were retrospectively analyzed.The detection rates of common gynecological diseases,cervical cancer and precancerous lesions and human papillo-mavirus(HPV)infection by cervical cytology,colposcopy and cervical biopsy were analyzed.[Re-sults]The HPV infection(mainly single infection)rate was 10.74％.The common gynecological diseases were vaginitis,cervicitis and uterine leiomyoma.The detection rate of cervical cancer was 19.59/105,and the detection rate of cervical precancerous lesions was 308.52/105.Rural resi-dents,HPV 16/18 infection,genital warts were the risk factors for cervical lesions.[Conclusion]Female cervical lesions are significantly correlated with HPV 16/18 infection,genital condyloma acuminatum,particularly for rural residents,and preventive care,early screening and interven-tion should be strengthened for these populations.

OBJECTIVE To analyze the factors influencing patient satisfaction in the outpatient pharmacy of tertiary maternity and child specialist hospitals in Chongqing,and provide a reference for improving the pharmaceutical management capability of tertiary maternity and child specialist hospitals and enhancing patients'medical experience.METHODS Utilizing KANO model,a questionnaire was developed and data were analyzed.Key influencing factors were identified through the categorization of requirement attributes,Better values,Worse values,and two-dimensional matrix analysis.The impact of these categorized demand factors on overall satisfaction was further validated through Structural Equation Modeling (SEM).RESULTS Cronbach's α coefficient for the survey questionnaire was 0.855,exceeding the acceptable threshold of 0.7;Bartlett test for sphericality yielded a value of 5538.56 with P<0.01,indicating good reliability and validity of the survey results.Through the KANO model's factor selection process,the top four key factors influencing patient satisfaction in outpatient pharmacies were determined to be:medication pick-up time (r=0.45),pharmacist service attitude (r=0.45),rational medication consultation (r=0.41),self-service calling system (r=0.40),all of which were subsequently validated through SEM.CONCLUSIONS The four factors of medication pick-up time,self-service calling system,pharmacist service attitude,and rational medication consultation significantly influence patient satisfaction in the outpatient pharmacies of tertiary maternity and child hospitals in Chongqing.

Gastric ulcer is a common digestive system disease,and the long-term use of non-steroidal anti-inflammatory drugs(NSAIDs)is the second most important cause.NSAID-induced gastric ulcer animal models are key experimental tools for studying the pathogenesis,corresponding treatment method,and effective mechanisms of NSAID-induced gastrointestinal injury.However,there are currently a lack of reviews on NSAID-induced gastric ulcer animal models.This review summarizes and compares the relevant literature on animal research into indomethacin-and aspirin-induced gastric ulcers in the past 10 years,including the selection of experimental animals,drug solvents,and specific modeling method.The limitations of current models,such as the cumbersome modeling method,incomplete modeling details,inadequate models for clinical use,and lack of comparative drug research,are discussed.Feasible solutions are proposed with the aim of providing an effective reference for research in this field.

Objective To employ a mouse model of ABI3BP gene deletion for the detection of postnatal changes in body weight and glucose metabolism and establish a different method of creating a mouse model of low birth weight.Methods Heterozygote mice were mated to produce ABI3BP gene knockout homozygote(ABI3BP-/-)mice,heterozygote(ABI3BP+/)mice,and wild-type(WT)mice.Adult mice from all three groups were evaluated for glucose metabolism markers,including the fasting blood glucose level,glucose tolerance,and insulin tolerance.Additionally,body weight was measured at various postnatal time periods,and the weight ratio of critical organs in adulthood was calculated.Results The gene sequencing result of the polymerase chain reaction product of ABI3BP-/-mice showed that frameshift mutations occurred in the knockout region,with quantitative reverse-transcription polymerase chain reaction analysis demonstrating significantly reduced ABI3BP expression in ABI3BP-/-mice compared with that in WT mice.Notably,the birth weight of ABI3BP-/-mice(1.25±0.08 g)was markedly lower than that of WT mice(1.34±0.12 g)(P＜0.05).Conversely,the weight of adult(120 d)ABI3BP-/mice(27.70±1.93 g)was significantly higher than that of WT mice(23.64±1.34 g)(P＜0.01).The ratios of key organ weights to body weight were not significantly different between the groups(P＞0.05).Fasting blood glucose and insulin tolerance tests showed no significant variations between the groups.However,glucose tolerance tests indicated that ABI3BP-/-mice had lower blood glucose levels(15.68±7.04 mmol/L)than WT mice(23.01±5.75 mmol/L).Conclusions Deletion of the ABI3BP gene result in mice with low birth weight,poor growth recuperation,and inadequate glucose tolerance in adulthood,similar to the clinical growth traits of low-birth-weight human neonates.Therefore,this mouse model is a promising choice for the study of low birth weight.