Objective To explore the effect of FBXW7 on ferroptosis in head and neck squamous cell carcinoma.Methods Head and neck squamous cell lines HN4 and HN6 were cultured in vitro.FBXW7 and SOX2 overexpression plasmids were constructed,and the plasmids were stably transfected into cell lines.The overexpression transfection efficiency was verified at the transcription level and protein level by qRT-PCR and Western blot experiments,respectively.The lipid peroxidation levels of head and neck squamous cell carcinoma cells with overexpressing FBXW7 were verified by measuring malondialdehyde(MDA),glutathione(GSH),and reactive ox-ygen species(ROS)levels.After treating cells with ferroptosis inhibitor Fer-1,the changes in cell viability were further detected to ver-ify the effect of FBXW7 on ferroptosis.The effect of transfection of the overexpressed plasmid on cellular pathways was detected by Western blot.Results HN4 and HN6 cell lines showed increased levels of lipid peroxidation after overexpression of FBXW7,and the ferroptosis inhibitor Fer-1 was able to effectively reverse the ferroptosis induced by overexpression of FBXW7.Western blot assay results showed that overexpression of FBXW7 reduced the expression of c-Myc,SOX2 and SLC7A11.Conclusion FBXW7 regulates the ex-pression of SOX2-SLC7A11 by degrading c-Myc,thereby effectively regulating ferroptosis in HNSCC.

Objective:To investigate the characteristics of fetal cardiac dysfunction in monochorionic diamniotic(MCDA)twin pregnancies,and establish a prediction model for their perinatal outcomes mainly based on ultra-sound indicators using information method.Methods:A total of 104 twin cases with ultrasound indicating fetal heart abnormalities were selected.Based on their chorionic and fetal heart abnormalities,they were divided into three groups:MCDA dysfunction group(61 cases),MCDA structural abnormalities group(23 cases),and dichori-onic diamniotic(DCDA)structural abnormalities group(20 cases).The clinical features of the three groups were analyzed and compared,and clinical outcomes were followed up until 3 years postpartum.A perinatal outcome prediction model for fetal cardiac dysfunction was established using the information method.All ultrasound abnor-mal indicators(7 categories)from the MCDA functional abnormality group,as well as 3 common clinical indicators,were included in the information model calculation.At the same time,the effectiveness of the model prediction was evaluated using receiver operating characteristic(ROC)curves and reserved cases(5 cases).Results:① Com-pared with the group with MCDA structural abnormalities,the group with MCDA functional abnormalities had a higher proportion of twin specific complications and a more severe staging.There was no statistically significant difference(P>0.05)in the proportion of live and non-live birth among the three groups,as well as the differences between different live birth outcomes and non-live birth outcomes.However,follow-up found that the majority(22/24)of live births in the MCDA dysfunction group had their heart function returning to normal on their own.②The pre-diction results of the information model showed that the classification criteria for adverse perinatal outcome or risks(I)of fetal cardiac dysfunction were:when I>1.0,the risk of fetal death was high;when-1.0

Objective:To investigate the characteristics of fetal cardiac dysfunction in monochorionic diamniotic(MCDA)twin pregnancies,and establish a prediction model for their perinatal outcomes mainly based on ultra-sound indicators using information method.Methods:A total of 104 twin cases with ultrasound indicating fetal heart abnormalities were selected.Based on their chorionic and fetal heart abnormalities,they were divided into three groups:MCDA dysfunction group(61 cases),MCDA structural abnormalities group(23 cases),and dichori-onic diamniotic(DCDA)structural abnormalities group(20 cases).The clinical features of the three groups were analyzed and compared,and clinical outcomes were followed up until 3 years postpartum.A perinatal outcome prediction model for fetal cardiac dysfunction was established using the information method.All ultrasound abnor-mal indicators(7 categories)from the MCDA functional abnormality group,as well as 3 common clinical indicators,were included in the information model calculation.At the same time,the effectiveness of the model prediction was evaluated using receiver operating characteristic(ROC)curves and reserved cases(5 cases).Results:① Com-pared with the group with MCDA structural abnormalities,the group with MCDA functional abnormalities had a higher proportion of twin specific complications and a more severe staging.There was no statistically significant difference(P>0.05)in the proportion of live and non-live birth among the three groups,as well as the differences between different live birth outcomes and non-live birth outcomes.However,follow-up found that the majority(22/24)of live births in the MCDA dysfunction group had their heart function returning to normal on their own.②The pre-diction results of the information model showed that the classification criteria for adverse perinatal outcome or risks(I)of fetal cardiac dysfunction were:when I>1.0,the risk of fetal death was high;when-1.0

Objective:To investigate the characteristics of fetal cardiac dysfunction in monochorionic diamniotic(MCDA)twin pregnancies,and establish a prediction model for their perinatal outcomes mainly based on ultra-sound indicators using information method.Methods:A total of 104 twin cases with ultrasound indicating fetal heart abnormalities were selected.Based on their chorionic and fetal heart abnormalities,they were divided into three groups:MCDA dysfunction group(61 cases),MCDA structural abnormalities group(23 cases),and dichori-onic diamniotic(DCDA)structural abnormalities group(20 cases).The clinical features of the three groups were analyzed and compared,and clinical outcomes were followed up until 3 years postpartum.A perinatal outcome prediction model for fetal cardiac dysfunction was established using the information method.All ultrasound abnor-mal indicators(7 categories)from the MCDA functional abnormality group,as well as 3 common clinical indicators,were included in the information model calculation.At the same time,the effectiveness of the model prediction was evaluated using receiver operating characteristic(ROC)curves and reserved cases(5 cases).Results:① Com-pared with the group with MCDA structural abnormalities,the group with MCDA functional abnormalities had a higher proportion of twin specific complications and a more severe staging.There was no statistically significant difference(P>0.05)in the proportion of live and non-live birth among the three groups,as well as the differences between different live birth outcomes and non-live birth outcomes.However,follow-up found that the majority(22/24)of live births in the MCDA dysfunction group had their heart function returning to normal on their own.②The pre-diction results of the information model showed that the classification criteria for adverse perinatal outcome or risks(I)of fetal cardiac dysfunction were:when I>1.0,the risk of fetal death was high;when-1.0

Objective:To investigate the characteristics of fetal cardiac dysfunction in monochorionic diamniotic(MCDA)twin pregnancies,and establish a prediction model for their perinatal outcomes mainly based on ultra-sound indicators using information method.Methods:A total of 104 twin cases with ultrasound indicating fetal heart abnormalities were selected.Based on their chorionic and fetal heart abnormalities,they were divided into three groups:MCDA dysfunction group(61 cases),MCDA structural abnormalities group(23 cases),and dichori-onic diamniotic(DCDA)structural abnormalities group(20 cases).The clinical features of the three groups were analyzed and compared,and clinical outcomes were followed up until 3 years postpartum.A perinatal outcome prediction model for fetal cardiac dysfunction was established using the information method.All ultrasound abnor-mal indicators(7 categories)from the MCDA functional abnormality group,as well as 3 common clinical indicators,were included in the information model calculation.At the same time,the effectiveness of the model prediction was evaluated using receiver operating characteristic(ROC)curves and reserved cases(5 cases).Results:① Com-pared with the group with MCDA structural abnormalities,the group with MCDA functional abnormalities had a higher proportion of twin specific complications and a more severe staging.There was no statistically significant difference(P>0.05)in the proportion of live and non-live birth among the three groups,as well as the differences between different live birth outcomes and non-live birth outcomes.However,follow-up found that the majority(22/24)of live births in the MCDA dysfunction group had their heart function returning to normal on their own.②The pre-diction results of the information model showed that the classification criteria for adverse perinatal outcome or risks(I)of fetal cardiac dysfunction were:when I>1.0,the risk of fetal death was high;when-1.0

Objective:To investigate the characteristics of fetal cardiac dysfunction in monochorionic diamniotic(MCDA)twin pregnancies,and establish a prediction model for their perinatal outcomes mainly based on ultra-sound indicators using information method.Methods:A total of 104 twin cases with ultrasound indicating fetal heart abnormalities were selected.Based on their chorionic and fetal heart abnormalities,they were divided into three groups:MCDA dysfunction group(61 cases),MCDA structural abnormalities group(23 cases),and dichori-onic diamniotic(DCDA)structural abnormalities group(20 cases).The clinical features of the three groups were analyzed and compared,and clinical outcomes were followed up until 3 years postpartum.A perinatal outcome prediction model for fetal cardiac dysfunction was established using the information method.All ultrasound abnor-mal indicators(7 categories)from the MCDA functional abnormality group,as well as 3 common clinical indicators,were included in the information model calculation.At the same time,the effectiveness of the model prediction was evaluated using receiver operating characteristic(ROC)curves and reserved cases(5 cases).Results:① Com-pared with the group with MCDA structural abnormalities,the group with MCDA functional abnormalities had a higher proportion of twin specific complications and a more severe staging.There was no statistically significant difference(P>0.05)in the proportion of live and non-live birth among the three groups,as well as the differences between different live birth outcomes and non-live birth outcomes.However,follow-up found that the majority(22/24)of live births in the MCDA dysfunction group had their heart function returning to normal on their own.②The pre-diction results of the information model showed that the classification criteria for adverse perinatal outcome or risks(I)of fetal cardiac dysfunction were:when I>1.0,the risk of fetal death was high;when-1.0

Objective:To investigate the characteristics of fetal cardiac dysfunction in monochorionic diamniotic(MCDA)twin pregnancies,and establish a prediction model for their perinatal outcomes mainly based on ultra-sound indicators using information method.Methods:A total of 104 twin cases with ultrasound indicating fetal heart abnormalities were selected.Based on their chorionic and fetal heart abnormalities,they were divided into three groups:MCDA dysfunction group(61 cases),MCDA structural abnormalities group(23 cases),and dichori-onic diamniotic(DCDA)structural abnormalities group(20 cases).The clinical features of the three groups were analyzed and compared,and clinical outcomes were followed up until 3 years postpartum.A perinatal outcome prediction model for fetal cardiac dysfunction was established using the information method.All ultrasound abnor-mal indicators(7 categories)from the MCDA functional abnormality group,as well as 3 common clinical indicators,were included in the information model calculation.At the same time,the effectiveness of the model prediction was evaluated using receiver operating characteristic(ROC)curves and reserved cases(5 cases).Results:① Com-pared with the group with MCDA structural abnormalities,the group with MCDA functional abnormalities had a higher proportion of twin specific complications and a more severe staging.There was no statistically significant difference(P>0.05)in the proportion of live and non-live birth among the three groups,as well as the differences between different live birth outcomes and non-live birth outcomes.However,follow-up found that the majority(22/24)of live births in the MCDA dysfunction group had their heart function returning to normal on their own.②The pre-diction results of the information model showed that the classification criteria for adverse perinatal outcome or risks(I)of fetal cardiac dysfunction were:when I>1.0,the risk of fetal death was high;when-1.0

Objective:To investigate the characteristics of fetal cardiac dysfunction in monochorionic diamniotic(MCDA)twin pregnancies,and establish a prediction model for their perinatal outcomes mainly based on ultra-sound indicators using information method.Methods:A total of 104 twin cases with ultrasound indicating fetal heart abnormalities were selected.Based on their chorionic and fetal heart abnormalities,they were divided into three groups:MCDA dysfunction group(61 cases),MCDA structural abnormalities group(23 cases),and dichori-onic diamniotic(DCDA)structural abnormalities group(20 cases).The clinical features of the three groups were analyzed and compared,and clinical outcomes were followed up until 3 years postpartum.A perinatal outcome prediction model for fetal cardiac dysfunction was established using the information method.All ultrasound abnor-mal indicators(7 categories)from the MCDA functional abnormality group,as well as 3 common clinical indicators,were included in the information model calculation.At the same time,the effectiveness of the model prediction was evaluated using receiver operating characteristic(ROC)curves and reserved cases(5 cases).Results:① Com-pared with the group with MCDA structural abnormalities,the group with MCDA functional abnormalities had a higher proportion of twin specific complications and a more severe staging.There was no statistically significant difference(P>0.05)in the proportion of live and non-live birth among the three groups,as well as the differences between different live birth outcomes and non-live birth outcomes.However,follow-up found that the majority(22/24)of live births in the MCDA dysfunction group had their heart function returning to normal on their own.②The pre-diction results of the information model showed that the classification criteria for adverse perinatal outcome or risks(I)of fetal cardiac dysfunction were:when I>1.0,the risk of fetal death was high;when-1.0

Objective:To investigate the effectiveness and safety of low-dose oral misoprostol solution for cervical ripening in late gestation.Methods:This was a prospective cohort study including 396 primiparas with singleton pregnancy who received low-dose oral misoprostol solution for cervical ripening (oral group) in Peking University Third Hospital from March to October 2022. They were further allocated to receive oral misoprostol alone (OA group, n=167) or oral misoprostol in combination with oxytocin/amniotomy (OC group, n=229). Moreover, 218 cases who received vaginal misoprostol for cervical ripening (vaginal group) during the same period in 2021 were reviewed (a retrospective cohort). Among them, 77 were given vaginal misoprostol alone (VA group) and 141 received vaginal misoprostol in combination with oxytocin/amniotomy (VC group). The OA group and VA group (72 and 73 cases) as well as the OC group and VC group (108 and 103 cases) were matched using propensity scores. Basic clinical information, hospital stay, duration of labor induction, uterine hyperstimulation, rate of labor initiation, vaginal delivery rate, rate of delivery within 24 h, duration of labor, neonatal condition, adverse pregnancy outcomes, and other information were compared between different groups. All data were statistically analyzed using independent sample t test, analysis of variance, nonparametric test, Chi-square test, or Fisher's exact probability test. Logistic regression model was used to analyze the factors affecting the labor initiation and the failure of labor induction. Results:The average hospital stay, the duration from medication to labor initiation and the duration from medication to vaginal delivery were significantly shorter in the oral group than those in the vaginal group [(5.4±2.4) vs. (6.5±2.6) d, (34.2±24.1) vs. (38.9±25.7) h, (45.8±25.8) vs. (53.4±27.8) h; t=5.24, 2.10 and 3.39; all P<0.05]. The total labor initiation rate and vaginal delivery rate in the oral group were significantly higher than those in the vaginal group [92.9% (368/396) vs. 83.5% (182/218), 72.2% (286/396) vs. 60.1% (131/218); χ 2=13.43 and 9.50; both P<0.05]. The incidence of failed induction of labor, uterine hyperstimulation, fetal distress, and intrauterine infection in the oral group were lower than those in the vaginal group [2.0% (8/396) vs. 6.9% (15/218), 4.3% (17/396) vs. 17.9% (39/218), 8.8% (35/396) vs. 14.7% (32/218), 1.3% (5/396) vs. 3.7% (8/218); χ 2=9.21, 31.36, 4.93 and 3.93; all P<0.05]. The duration from medication to labor initiation and to vaginal delivery in the OA group were higher than those in the VA group [(25.8±17.0) vs. (17.4±10.8) h, (37.2±18.8) vs. (29.7±13.5) h; t=3.49 and 2.74; both P<0.05]. There were no significant differences in the labor initiation rate, vaginal delivery rate, rate of delivery within 24 h or the incidence of failed induction of labor between the OA and VA groups (all P>0.05). Women in the VA group were more likely to develop uterine hyperstimulation than those in the OA group [19.2% (14/73) vs. 4.2% (3/72), χ2=7.89, P=0.005]. There were no significant differences in the duration from medication to labor initiation or to vaginal delivery between the VC and OC groups (both P>0.05), but the duration were significantly longer than those in the corresponding medication alone group (VC vs. VA groups: (49.7±24.6) vs. (17.4±10.8) h and (61.6±25.7) vs. (29.7±13.5) h, t=5.31 and 5.13, both P<0.05; OC vs. OA groups: (45.3±26.6) vs. (25.8±17.0) h and (56.1±27.2) vs. (37.2±18.8) h, t=10.35 and 9.78, both P<0.05]. The labor initiation rate, vaginal delivery rate and rate of delivery within 24 h in the OC group were higher than those in the VC group [88.9% (96/108) vs. 77% (87/113), 63.0% (68/108) vs. 47.8% (54/113), 10.3% (7/108) vs. 0.0% (0/113); χ 2=5.49, 5.14 and 7.56; all P<0.05]. The incidence of uterine hyperstimulation in the OC group was 4.6% (5/108), which was lower than that in the VC group [18.6% (21/113), χ 2=10.37, P=0.001]. Logistic regression analysis showed that oral misoprostol and gestational age were positively correlated with labor initiation [ OR (95% CI): 2.18 (1.24-3.90) and 1.43 (1.14-1.79)], while maternal age was negatively correlated with labor initiation [ OR (95% CI): 0.90 (0.82-0.98)]. Moreover, failed induction of labor was negatively correlated with oral misoprostol [ OR (95% CI): 0.37 (0.14-0.91)], but positively correlated with maternal age [ OR (95% CI): 1.21 (1.05-1.40)]. Conclusions:Oral administration of low-dose misoprostol solution is as effective as vaginal misoprostol in promoting cervical ripening. Besides, it can shorten the average hospital stay and reduce the incidence of uterine hyperstimulation, suggesting that low-dose oral misoprostol solution is relatively safer and can be used to promote cervical ripening in late gestation.

The pathogenesis of brain injury in fetal growth restriction (FGR) fetuses is likely associated with oxidative stress and neuroinflammation, although the exact mechanisms are not fully understood. This article mainly reviews the anatomical alterations, potential pathophysiological processes, and the specific molecular mechanisms involving various types of brain cells in FGR.