Objective To investigate the current situation of treatment and control of hypertension among the elderly in Jiang^’an District of Wuhan, and to analyze the relationship between body mass index (BMI), waist circumference (WC), waist-to-height ratio (WHtR) and the treatment and control of hypertension. Methods A random sampling method was used to recruit 17,749 hypertension patients aged 65 and above who participated in free physical examinations for the elderly in Jiangan District. Relevant information was collected through questionnaire surveys and physical examinations in 2018. Logistic regression analysis was conducted to analyze the correlation between BMI, WC, WHtR and the treatment and control rate of hypertension. Results Among all elderly hypertension patients included in this study, 12,379 (69.74%) reported the treatment of hypertension, of which 6,055(48.91%) had their high blood pressure under control. After adjusting factors such as gender, age, and education level, etc., obesity (OR=1.698), central obesity (OR=1.445), and abnormal WHtR (OR=1.421) were the protective factors of the treatment of hypertension. Overweight (OR=0.843), obesity (OR=0.762) and high WHtR（OR=0.889）reduced the control rate of hypertension. There was no significant relationship between abdominal obesity and blood pressure control. Conclusion Overweight，obesity and high WHtR are the risk factors of hypertension control in the elderly. Weight management can therefore be a takeaway as a key intervention measure for hypertension management in the elderly.

Objective To deveplope construct and validate a novel multiplex PCR system comprised of 30 Y-STR markers only with low and moderate mutation rates. Methods 30 Y-STRs characterized by low/moderate mutation rate and middle/high polymorphic was amplified simultaneously in a multiplex PCR system using the six color labeling fluorescence. PCR product was analyzed in a ABI 3500XL Genetic Analyzer. The accuracy, specifity, sensitivity and stability of the system and its validation on the mixtures were evaluated. Results The validation studies demonstrated that the system is a stable, accurate, and sensitive multiplex PCR system. The sensitivity was 0.0625ng DNA. Y-STR could be detection in a male/female DNA mixture ratio of 1:4. Conclusion The primary study demonstrates that this multiplex PCR system is effective and reliable for forensic routine DNA analysis. It will be very helpful for constructing Chinese forensic Y-STR database and population genetic research.

This paper reviewed recent advances in clinical and basic researches of scalp acupuncture based on evoked potentials (EPs).The role and significance of various EPs in scalp acupuncture were analyzed and compared.Eventrelated potentials (ERPs) which occupied leading position in EPs application nowadays and the latest advances in the related components of its action were combined to make a prospect of the scientific research methods of scalp acupuncture in the treatment of cerebral apoplexy and other diseases.

This study was aimed to explore the immediate effect mechanism of the middle 2/5 of the vertex-temporal anterior oblique line in the treatment of stroke by scalp acupuncture using event-related brain potential (ERP).A total of 30 stroke cases who met the inclusion criteria and 25 healthy subjects were selected.Patients in both groups were treated by scalp acupuncture,with stimulation on the middle 2/5 of the vertex-temporal anterior oblique line on the right side.ERP was detected before acupuncture and 10 min after acupuncture in both groups.The muscle strength and neurological deficit score of the stroke group were also detected.At the end of the experiment,the movement-related cortical potentials (MRCPs) of both the left and right hand were extracted from the ERP.The results showed that after 10 min scalp acupuncture stimulation,the neurological deficit score of the stroke group was lower than that of the pretreatment (P ＜ 0.05);the muscle strength rating was higher than before (P ＜ 0.05).Before and after acupuncture,the amplitude of MRCPs on the diseased side of the stroke group was significantly decreased compared to before (P ＜ 0.05).It was concluded that the immediate effect of scalp acupuncture treatment of hemiplegia patients with stroke was definite.Its mechanism may be that acupuncture stimulates the motor cortex through the vertex-temporal anterior oblique line,reduces the energy loss of brain motion planning,activates the damaged brain movement related area cortex neurons,improves the processing efficiency of movement in the brain,so as to promote the improvement of hemiplegic limb motor function immediately.And we also observed that the healthy side brain has a compensatory effect,which suggested that in our clinical scalp acupuncture,bilateral stimulation may be more effective.

Objective:To study the incidence, clinical features, diagnosis, and treatment of angioimmunoblastic T-cell Lymphoma (AITL). Methods:We retrospectively analyzed the clinical data of 31 patients with AITL treated in the tumor hospital of Harbin Medical University between January 1995 and January 2008. We also analyzed the incidence, clinical features, diagnosis, and treatment of AITL. Results:The complete response rate and partial response rate of the entire group were 54.8%and 16.1%, respectively. The overall response rate was 70.9%, and the median survival time was 27.3 months. The five-year survival rate of the entire group was 35%. The response rate was 76.5%in the CHOP group and 75%in the COP group. No significant differences were detected between two groups (P>0.05). Con-clusion:The symptoms and signs of ATIL differ in different sites. The COP and CHOP regimens have similar efficacy and tolerable side effects.

Objective To explore and analyze the clinical efficacy and effects of the application of dexmedetomidine in the tube drawing period of general anesthesia for the patients receiving laparoscopic surgery. Methods Sixty-eight pa-tients who received laparoscopic surgery in our hospital from December 2012 to December 2013 were selected as the study subjects and randomly divided into the control group and the study group. By the end of surgery, the patients were transferred to the post-anesthesia care unit (PACU) with catheter and different anesthetic drugs were used. The control group received target controlled infusion of propofol and the study group received dexmedetomidine. Results The four groups of patients were different in MAP and BIS at T2, T3, T4 and T5, with statistically significant differ-ences (P<0.05). Regarding to VAS scores and adverse reactions, group A2 and group A3 were different from the control group respectively, with significant differences (P<0.05). Conclusion The application of dexmedetomidine in the tube drawing period of general anesthesia after laparoscopic surgery for the purpose of analgesia and sedation is conducive to maintaining hemodynamic stability, relieving postoperative pain and reducing the incidence of postoperative complica-tions.

Objective To observe and analyze the clinical analgesic anesthesia effect of propofol combined with pare-coxib sodium for the patients receiving bronchofiberscope examination. Methods Eighty-seven patients receiving bron-chofiberscope examination in our hospital from January 2012 to February 2014 were selected as the study subjects and randomly divided into group A, group B and group C. The three groups of patients received parecoxib sodium combined with propofol, propofol combined with fentanyl, and propofol respectively for preoperative anesthesia. The MAP and SPO2 changes, usage amount of anesthetic drugs, and awakening time of the three groups at different time were recorded, and the results were tabulated and analyzed. Results SpO2 of the three groups at different time did not change obvi-ously, with no significant differences (P>0.05). The three groups did not have statistically significant differences in the bronchofiberscope examination time (P>0.05). Group A had the lowest usage amount of anesthetic drugs and the short-est awakening time, which had statistically significant differences to the other two groups (P<0.05). Conclusion In analgesic bronchofiberscope examination, the application of propofol combined with parecoxib sodium for anesthesia and analgesia plays a positive role in increasing the patients' postoperative comfort sensation and postoperative living quality.

Objective To investigate the RADIL mRNA expression in pancreatic carcinoma and to evaluate its clinical significance.Methods Fluoesecent quantitative PCR (FQ-PCR) was used to detect the RADIL mRNA expression in 40 patients with pancreatic carcinoma and adjacent tissue and in 5 healthy adult with normal pancreatic tissue and to observe its relationship with clinicopathologic parameters.Results RADIL mRNA was expressed in pancreatic carcinoma and adjacent tissue, as well as normal pancreatic tissue, and the relative expression was 2.263 ± 3.826, 5.425 ± 8.858 and 8.559 ± 4.214, respectively.There was statistically significant difference among the three groups (P ＜0.05 ).RADIL mRNA expression was closely related with the metastasis and differentiation grade ( r = -0.312 and -0.294, P ＜ 0.05 ), however, it was not significantly related to tumor site, tumor size, CA19-9, TNM staging, sex and age.Conclusions RADIL gene may have an inhibitory effect on the pancreatic cancer.

Objective To investigate the methylation status of PCDH8 gene in pancreatic carcinoma.Methods Methylation of PCDH8 gene in 2 samples of normal pancreatic tissues and 6 pancreatic carcinoma cell lines (PANC1, ASPC1, BxPC3, CFPAC, PaTu8988 and SW1990) was detected by the methylationspecific PCR (MSP) method. The expression of PCDH8 mRNA was detected with 5-Aza-2-deoxycytidine (5-Aza-dC) treatment, a kind of DNA methyltransferase (DNMT) inhibitor in 6 pancreatic carcinoma cell lines by real-time-PCR. Results The methylation of PCDH8 gene was not detected in normal tissues, while it was partially methylated in PANC1, BxPC3, CFPAC and it was totally methylated in PaTu8988, ASPC1, SW1990.PCDH8 mRNA was expressed in PANC1, SW1990, PaTu8988 and the relative quantities of mRNA expression (RQ) were 1.576 ± 0.648, 0.013 ± 0.008, 0.002 ± 0.001; PCDH8 mRNA was not expressed in BxPC3,CFPAC, ASPC1. After 5-Aza-dC treatment, PCDH8 mRNA was expressed in PANC1, ASPC1, BxPC3,CFPAC, PaTu8988, SW1990 and the relative quantities of mRNA expression all significantly increased, and they were 7. 463 ± 2.628, 10. 696 ± 1.539, 7.852 ± 2.762,421.815 ± 1.493, 118.595 ± 4.089, 6.690 ±1.884. Conclusions The methylation of PCDH8 gene may be the major mechanism of down-regulated expression of PCDH8 gene in pancreatic carcinoma.

[Objective] To learn about the genetic diversity,we studied the five X-chromosomal STR (X-STR) loci in Guangdong Han Nationality Groups.[Methods] The five Loci (DXS6803,DXS981,DXS6809,DXS6789,and DXS7132) were amplified in a pentaplex PCR reaction.PCR products were analyzed using capillary electrophoresis and ABI prism 3100 Genetic Analyzer,with GeneMapper ID 3.1 Analysis Software.[Results] A total of 363 individuals (181 unrelated male and 182 unrelated female) from Guangdong Han population were tested,54 alleles were observed for these loci.Polymorphism information content is 0.6935 ～ 0.8177.Power of discrimination in females was 0.8976 ～ 0.9562.Mean exclusion chance for X-STR in standard trios with daughters was 0.7805 ～ 0.8467.[Conclusion] The five loci in the multiplex system provide high polymorphism information for forensic identification and paternity testing,particularly for difficult paternity deficiency cases.