Objective: To analyze the infection status of human papillomavirus (HPV) among women aged 35-64 years in Deqing County, Zhejiang Province, so as to provide a reference for the prevention and treatment of cervical lesions. Methods: Women aged 35-64 years who underwent combined cervical cancer screening with HPV and ThinPrep cytologic test (TCT) at the Deqing County Maternal and Child Health and Family Planning Service Center from 2022 to 2024 were selected as study participants. The HPV infection rate and subtype distribution, positive rate of epithelial cell abnormalities, and positive rate of cervical lesions were analyzed. Results: A total of 16 155 women were included, with the 55-<60 years age group being the most prevalent, accounting for 8 200 (50.76%). HPV infection was detected in 2 015 cases, with an infection rate of 12.47%. High-risk HPV infection was detected in 1 735 cases (86.10%), with an infection rate of 10.74%. Low-risk HPV infection was detected in 280 cases (13.90%), with an infection rate of 1.73%. The top three HPV subtypes with the highest infection rates were HPV52, 58, and 16, with infection rates of 3.46%, 1.84%, and 1.18%, respectively. The rates of high-risk HPV infection, single infection, and multiple infection all showed an upward trend with increasing age (all P<0.05), peaking in the group aged 60-64 years, at 13.26%, 10.96%, and 2.30%, respectively. The positive rate of cervical epithelial cell abnormalities in women with high-risk HPV infection was higher than in women with low-risk HPV infection (27.43% vs. 2.86%, P<0.05). Among them, the positive rates of low-grade squamous intraepithelial lesions and atypical squamous cells of undetermined significance were higher in women with high-risk HPV infection than in women with low-risk HPV infection (both P<0.05). Cervical lesions were detected in 150 cases, with a positive rate of 8.65%. Conclusions The HPV infection rate among women aged 35-64 years in Deqing County was at a moderate level nationally, with HPV52, 58, and 16 being the predominant subtypes. The infection rate increases with age, highlighting the need for enhanced cervical lesions screening among women with high-risk HPV infection.

Objective To analyze the effects of different therapies on patient survival,to explore the related prognostic factors in elderly patients with advanced gastric cancer,and to provide recommendations for the treatment of such patients.Methods Retrospective analysis was conducted on 146 elderly patients with advanced gastric cancer hospitalized from January 2009 to October 2013 in Yixing People's Hospital of Jiangsu Province.Detailed clinical data were recorded,and patients were followed up during the total survival time.Univariate analysis with the Log rank test and multivariate analysis with the COX proportional hazard model were utilized to examine the related prognostic factors in elderly patients with advanced gastric cancer.Results The 1-,2,3-year survival rates of t46 elderly patients with advanced gastric cancer were 33.6 ％,11.0 ％,and 2.1 ％,respectively,and the median survival time was 10.3 months.The Log-rank test showed that Karnofsky (KPS) score,differentiation degree,number of metastatic sites,malignant serous effusion,chemotherapy,and traditional Chinese medicine (TCM) were associated with the prognosis of elderly patients with advanced GC (all P＜0.05).Multivariate analysis by the COX proportional hazard model showed that KPS score (HR=1.575,95％ CI:1.094 2.267,P=0.015),differentiation degree (HR=0.499,95％CI:0.340-0.732,P＜0.001),malignant serous effusion (HR=0.516,95％ CI:0.356-0.748,P＜ 0.001),chemotherapy (HR=1.669,95％ CI:1.185 2.351,P=0.003),and TCM (HR=1.793,95％ CI:1.237-2.600,P=0.002) were independent factors related to the prognosis of elderly patients with advanced GC.Conclusions The prognosis of elderly patients with advanced gastric cancer is poor,especially for patients with a low KPS score,a poor differentiation degree,or malignant serous effusion.Chemotherapy and TCM can improve the prognosis.

Objective To evaluate the effect of recombinant human tumor necrosis factor receptor type Ⅱ:IgG Fc fusion protein (rhTNFR:Fc,trade name Etanercept) combined with methotrexate on levels of interleukin-17A (IL-17A) and tumor necrosis factor-α (TNF-α) in the serum and mononuclear cells of patients with moderate to severe plaque psoriasis.Methods A total of 30 patients with moderate to severe plaque psoriasis were enrolled from Department of Dermatology of Tenth People's Hospital of Tongji University between August 2014 and February 2016,and then were randomly and equally divided into Etanercept group and Etanercept + methotrexate group.The treatment lasted 24 weeks.Fifteen healthy blood donors served as healthy control group.Enzyme-linked immunosorbent assay (ELISA) and real-time quantitative PCR were performed to measure the serum levels and mRNA expression of IL-17A and TNF-α,respectively,in the patients of the above two groups before and after the treatment.Results Before the treatment,the serum levels of IL-17A and TNF-ct,as well as the mRNA expression of IL-17A and TNF-α in peripheral blood mononuclear cells (PBMCs),were all significantly higher in all the patients than in the healthy controls (all P ＜ 0.05).After the treatment,compared with the Etanercept group,the Etanercept + methotrexate group showed significantly lower serum levels of IL-17A (142.67 ± 14.82 vs.163.54 ± 23.18,P ＜ 0.05) and TNF-α (70.07 ± 25.02 vs.91.98 ± 14.62,P ＜ 0.05),as well as lower mRNA expression of IL-17A (1.12 ± 0.33 vs.1.56 ± 0.77,P ＜ 0.05) and TNF-α in PBMCs (2.50 ± 1.04 vs.3.61 ± 2.14,P ＜ 0.05).Conclusion Etanercept combined with methotrexate is superior to Etanercept alone in the treatment of psoriasis,and can reduce treatment duration and improve therapeutic effect,likely by down-regulating the expression of IL-17A and TNF-α.

Objective To research the mechanism or pathway through which Substance P(SP) inhibits r-aminobutyric acid(GABA) activated currents in bullfrog dorsal root ganglion(DRG) neurons.Method The experiment was conducted on freshly isolated bullfrog dorsal root ganglion neurons using a whole-cell patch-clamp technique.Results SP could caused a slow inward current when SP was applied to DRG neurons;SP could inhibits GABA-activated currents;The inhibition could be reduced largely when protein kinase C(PKC) inhibiter,1-(5-isoquinolinesulphorry)-2-methylpiperazine(H-7), was dialyzed in cell body.Conclusion The SP ton inhibit GABA-activated currents through protein kinase C.