Objective : To examine the relationship between the levels of endothelin-1(ET-1) and the severity of clinical symptoms in idiopathic inflammatory demyelinating diseases(IIDDs) of the central nervous system. Methods : A total of 45 patients with central nervous system demyelinating diseases were enrolled in the study. Among them, 25 patients were diagnosed with idiopathic IIDDs, 20 had vascular demyelinating diseases of the central nervous system, and 10 healthy controls were also included. Serum ET-1 levels were assessed using ELISA, and a further analysis was conducted to investigate the correlation between these ET-1 levels, laboratory test results, and the degree of disease disability in the IIDDs patient group. Results: (1) Of the three groups, ET-1 levels were highest in the IIDDs(Ps=0.503, P=0.010), CSF total protein(rs=0.475, P=0.016), CSF albumin(rs=0.480, P=0.020), CSF IgG(rs=0.544, P=0.007), IgA(rs=0.660, P=0.002) and IgM(rs=0.555, P=0.011) levels.(3) There was a positive correlation between serum ET-1 levels and CSF IgM levels(rs=0.455, P=0.044) in IIDDs group. Serum ET-1 level showed no significant correlation with peripheral immune reaction(P>0.05). Conclusion Serum ET-1 levels reflect the severity of clinical symptoms in IIDDs and show no significant correlation with peripheral immune markers, however, exhibit a positive correlation with disease severity and cerebrospinal fluid IgM levels. These findings suggest that serum ET-1 levels may indicate the degree of central nervous system inflammation and play an important role in the development and progression of IIDDs.

Objective:To investigate the effects of human glycolipid transfer protein(GLTP)on proliferation,migration and invasion of pancreatic cancer(PC)cells,and to elucidate their mechanisms.Methods:The difference in the expression levels of GLTP proteins between PC tissue and normal pancreas tissue was analyzed by University of Alabama at Birmingham Cancer Data Analysis Platform(UALCAN)Database,as well as the difference in the expression levels of GLTP protein between PC tissue and normal pancreas tissue of the PC patients with different clinicopathological characteristics.The PANC-1 cells were cultured in vitro and divided into control group(transfected with pFLAG-CMV4 plasmid)and GLTP-overexpression(GLTP-OE)group(transfected with pFLAG-GLTP plasmid).The stably GLTP transfected cells were established using the antibiotic screening method.Knock-down experiments were performed using non-specific siRNA transfected PANC-1 cells as control group and si-GLTP transfected PANC-1 cells as si-GLTP group.Western blotting method was used to detect the expression of GLTP protein in the cells in various groups,cell counting kit-8(CCK-8)method was used to detect the proliferation activities of PANC-1 cells,clone formation assay was used to detect the number of clone formation,and Transwell chamber assay were used to detect the numbers of migration and invasion cells in various groups.Transcriptomics sequencing analyses were conducted to assess the possible mechanism of GLTP in the PANC-1 cells.Western blotting method was employed to detect the expression levels of phosphatidylinositol 3-kinase(PI3K),phosphorylated PI3K(p-PI3K),protein kinase B(Akt),and phosphorylated Akt(p-Akt)proteins in the PANC-1 cells in various groups;Real-time fluorescence quantitative PCR(RT-qPCR)was used to assess the expression levels of amphiregulin(AREG)and kinase insertion domain receptor(KDR)mRNA in the cells in various groups.The mice were randomly divided into control group(injected with pFLAG-GMV4 transfected PANC-1 cells)and experimental group(injected with pFLAG-GLTP stably transfected PANC-1 cells),and the subcutaneously transplanted tumor models were prepared;the volumes and weights of the transplanted tumors of the mice in two groups were measured.Results:UALCAN database analysis showed that the expression level of GLTP protein in PC tissue was lower than that in normal pancreas tissue(P＜0.01),and there were statistically significant differences in the GLTP protein expression between PC tissue and normal pancreas tissue of the PC patients with different cancer stages(P＜0.05),tumor grades(P＜0.05),ages(P＜0.001),and genders(P＜0.05).Compared with control group,the proliferation activity(P＜0.01)and the number of clone formation(P＜0.001)of the cells in GLTP-OE group were decreased,while the numbers of migration cells(P＜0.001)and invasion cells(P＜0.01)were decreased.In the knock-down experiment,compared with control group,the proliferation activity(P＜0.01)and the number of clone formation(P＜0.05)of the cells in GLTP-OE group were increased,while the numbers of migration cells(P＜0.001)and invasion cells(P＜0.001)were increased.Compared with control group,the tumor weight and volume of the mice in experimental group were decreased(P＜0.01),following the injection of tumor cells for a period of four weeks.In the over-expression experiment,compared with control group,the expression levels of p-PI3K(P＜0.01),p-Akt-S473(P＜0.01),and p-Akt-T308(P＜0.05)proteins in the cells in GLTP-OE group were decreased;the expression levels of AREG(P＜0.01)and KDR(P＜0.01)mRNA were decreased.In the knock-down experiment,compared with control group,the expression levels of p-PI3K(P＜0.01),p-Akt-S473(P＜0.01),and p-Akt-T308(P＜0.01)in the cells in si-GLPT group were increased,and the expression levels of AREG(P＜0.01)and KDR(P＜0.05)mRNA were increased.Conclusion:Low expression levels of GLTP in PC tissue are present.The over-expression of GLTP can inhibit the proliferation,migration and invasion of PANC-1 cells,as well as the growth of subcutaneously transplanted tumors in the nude mice;its possible mechanism may be related to decreasing the activity of the PI3K/Akt signaling pathway.

Objective To explore the clinical features and prognostic factors of adult hemophagocytic syndrome(HPS).Methods The clinical data of 68 newly diagnosed patients with HPS who were hospitalized from December 2018 to December 2023 were retrospectively analyzed,and clinical features,treatment and prognostic factors of patients were analyzed.Results The median age of 68 patients was 49(17-75)years old,the most common cause was infection,40 cases(58.82％),and the most common clinical manifestation was fever,63 cases(92.6％).Of the 68 patients,12(17.65％)patients died and the median survival time was 32 months.Cox univariate analysis showed that the prognosis of adult HPS was affected by SF,epstein-barr virus(EBV)infection,platelet count(＜20× 109/L),age over 60 years old,activated partial thromboplastin over 60s and other factors(P＜0.05).Multivariate Cox analysis showed that EBV infection and platelet count(＜20×109/L)were independent prognostic factors of adult HPS(P＜0.05).Conclusion The clinical manifestations of adult HPS are not typical,and the prognosis of adult HPS is affected by many factors.EBV infection and platelet count(＜20× 109/L)are independent risk factors for death.

OBJECTIVE: To review clinical application and research progress of different types of intelligent responsive hydrogels in repairing articular cartilage injury. METHODS: The animal experiments and clinical studies of different types of intelligent responsive hydrogels for repairing articular cartilage injury were summarized by reviewing relevant literature at home and abroad. RESULTS: The intrinsic regenerative capacity of articular cartilage following injury is limited. Intelligent responsive hydrogels, including those that are temperature-sensitive, light-sensitive, enzyme-responsive, pH-sensitive, and other stimuli-responsive hydrogels, can undergo phase transitions in response to specific stimuli, thereby achieving optimal functionality. These hydrogels can fill the injured cartilage area, promote the proliferation and differentiation of chondrocytes, and expedite the repair of the damaged site. With advancements in cartilage tissue engineering materials research, intelligent responsive hydrogels offer a novel approach and promising potential for the treatment of cartilage injuries. CONCLUSION Intelligent responsive hydrogel is a kind of flexible, controllable, efficient, and stable polymer, which has similar structure and functional properties to articular cartilage, and has become one of the important biomaterials for cartilage repair. However, there is still a lack of unified treatment standards and simple and efficient preparation technology.
Hydrogels/therapeutic use* ; Cartilage, Articular/injuries* ; Tissue Engineering/methods* ; Humans ; Animals ; Chondrocytes/cytology* ; Biocompatible Materials/chemistry* ; Tissue Scaffolds/chemistry*

Objective To investigate the relationships between adolescents'body mass index(BMI),blood pressure(BP),and myopia and provide a scientific basis for the formulation of myopia prevention and control measures.Methods Adolescent students who par-ticipated in physical fitness surveillance in Chenghua District,Chengdu,from 2014 to 2023 were selected as the study population.A cross-sectional study was conducted using multiple logistic regression,adjusting for age,sex,BP,and pulse as covariates.A Cox model was employed to examine the relationship between obesity and myopia.Additionally,for the 2023 dataset,characterized by the largest sample size and most complete data,a mediation analysis was performed with mean BP as the mediator.Results Annual cross-sectional analysis from 2014 to 2023 revealed a significant positive association between obesity and myopia,with OR and 95％CI of 1.16(1.10-1.22),1.15(1.08-1.21),1.19(1.12-1.26),1.11(1.04-1.18),1.10(1.05-1.15),1.10(1.05-1.16),1.07(1.03-1.12),1.10(1.05-1.15),1.12(1.07-1.17),and 1.11(1.06-1.15),respectively.Similarly,cohort analysis showed a significant positive association between obesity and myopia(HR=1.09,95％CI:1.01-1.19).Mediation analysis indicated that BP accounted for 21.2％of the total effect of obesity on myopia.Conclusion Obesity facilitates the onset of myopia,and weight reduction can help decrease the likelihood of latter.Furthermore,BP mediates the impact of obesity on myopia.Implementing BP-lowering interventions in obese populations may aid in the prevention of myopia.

Objective To explore the clinical features and prognostic factors of adult hemophagocytic syndrome(HPS).Methods The clinical data of 68 newly diagnosed patients with HPS who were hospitalized from December 2018 to December 2023 were retrospectively analyzed,and clinical features,treatment and prognostic factors of patients were analyzed.Results The median age of 68 patients was 49(17-75)years old,the most common cause was infection,40 cases(58.82％),and the most common clinical manifestation was fever,63 cases(92.6％).Of the 68 patients,12(17.65％)patients died and the median survival time was 32 months.Cox univariate analysis showed that the prognosis of adult HPS was affected by SF,epstein-barr virus(EBV)infection,platelet count(＜20× 109/L),age over 60 years old,activated partial thromboplastin over 60s and other factors(P＜0.05).Multivariate Cox analysis showed that EBV infection and platelet count(＜20×109/L)were independent prognostic factors of adult HPS(P＜0.05).Conclusion The clinical manifestations of adult HPS are not typical,and the prognosis of adult HPS is affected by many factors.EBV infection and platelet count(＜20× 109/L)are independent risk factors for death.

Objective To investigate the relationships between adolescents'body mass index(BMI),blood pressure(BP),and myopia and provide a scientific basis for the formulation of myopia prevention and control measures.Methods Adolescent students who par-ticipated in physical fitness surveillance in Chenghua District,Chengdu,from 2014 to 2023 were selected as the study population.A cross-sectional study was conducted using multiple logistic regression,adjusting for age,sex,BP,and pulse as covariates.A Cox model was employed to examine the relationship between obesity and myopia.Additionally,for the 2023 dataset,characterized by the largest sample size and most complete data,a mediation analysis was performed with mean BP as the mediator.Results Annual cross-sectional analysis from 2014 to 2023 revealed a significant positive association between obesity and myopia,with OR and 95％CI of 1.16(1.10-1.22),1.15(1.08-1.21),1.19(1.12-1.26),1.11(1.04-1.18),1.10(1.05-1.15),1.10(1.05-1.16),1.07(1.03-1.12),1.10(1.05-1.15),1.12(1.07-1.17),and 1.11(1.06-1.15),respectively.Similarly,cohort analysis showed a significant positive association between obesity and myopia(HR=1.09,95％CI:1.01-1.19).Mediation analysis indicated that BP accounted for 21.2％of the total effect of obesity on myopia.Conclusion Obesity facilitates the onset of myopia,and weight reduction can help decrease the likelihood of latter.Furthermore,BP mediates the impact of obesity on myopia.Implementing BP-lowering interventions in obese populations may aid in the prevention of myopia.

Objective: To explore the causal relationship between the abundance of intestinal flora(IF) and the risk of developing acute myeloid leukemia(AML) using two-sample bidirectional Mendelian randomization(MR) methods. Methods: The MiBioGen Consortium and FinnGen databases were utilized for the IF abundance and AML genome-wide association datasets, respectively. The primary analysis method entailed variance inverse weighting, supplemented by weighted median test, while heterogeneity was assessed using Cochran′sQtest. Additionally, MR-PRESSO was employed to identify any outliers, while MR-Egger intercept was utilized to evaluate horizontal pleotropy. Results: Higher abundance of genetically determinedDialister(P=0.034,OR=4.373,95%CI:1.329-14.392),Veillonaceae(P=0.009,OR=2.029,95%CI:1.839-3.866), andLachnospiraceae UCG008(P=0.034,OR=3.827,95%CI:1.107-13.228) were found to be linked to an increased risk of AML. In contrast,Ruminococcaceae(P=0.042,OR=0.176, 95%CI:0.033-0.939) andPeptococcaceae(P=0.025,OR=0.168, 95%CI:0.035-0.803) were associated with a reduced risk of this disease. Conclusion Higher levels of theDialister, theVeillonellaceae, and theLachnospiraceae UCG008are risk factors for AML, while the familiesRuminococcaceaeandPeptococcaceaeare identified as protective factors against AML.

Objective To evaluate the testing capabilities of laboratories,analyze existing issues,and improve testing quality,through carrying out the external quality assessment(EQA)of clinical laboratories for human papillomavirus(HPV)type 6 and 11 nucleic acid detection.Methods EQA plan was carried out twice a year.Each panel contains 4 positive samples,including one strong positive sample and one weak positive sample of HPV6 and HPV11,made from cervical secretions from patients with clinical manifestations of condyloma acuminata(CA)and positive for HPV6 or HPV11(from Shanghai First Maternity and Infant Hospital).One negative sample was cultured from the C-33A cell line(from Chinese Academy of Sciences).Samples were sent to participating laboratories by cold chain,and laboratories were required to detect test samples and upload their results within the specified time.Shanghai Center for Clinical Laboratory(SCCL)calculated the scores of each laboratory based on the return results.Results A total of 163 sample panels were sent out in the 6 rounds of EQA plan and 140 valid reports were received.The laboratory qualification rate was 96.43%(135/140)and the sample compliance rate was 97.86%(685/700).There were 13 false negative results and 2 false positive results,with weakly positive samples accounting for 76.92%(10/13)of the false negative results.Conclusion The detection accuracy of HPV6/11 nucleic acid in each laboratory was relatively high,and the detection ability of weak positive samples in individual laboratories may need to be improved.The laboratory could discover problems and improve its quality management by participating in EQA.

Objective:To investigate the effects of different levels of ophthalmic surgical stimulation on blood glucose in patients with type 2 diabetes mellitus(T2DM).Methods:From Mar to Oct 2021,236 patients with T2DM who underwent ophthalmic surgery in our hospital were enrolled,including 71 cases of secondary surgery,89 cases of tertiary surgery,and 76 cases of quaternary surgery.According to the operation time,the 236 patients were divided into groups A(＜60 min),B(60-120 min)and C(＞120 min).The preoperative and postoperative blood glucose levels were compared in patients with different levels of surgery,and in groups A,B and C.Results:The postoperative blood glucose level was lower than that before surgery in secondary and tertiary surgery,and it was higher than that before surgery in quaternary surgery(P＜0.05).The fluctuation value of blood glucose in secondary and tertiary surgery was higher than that in quaternary surgery(P＜0.05).In groups A,B and C,the postoperative blood glucose level was lower than that before surgery in secondary and tertiary surgery(P＜0.05).In group A,there was no significant difference in the blood glucose before and after surgery in quaternary surgery(P＞0.05),and in groups B and C,the postoperative blood glucose was higher than that before surgery in quaternary surgery(P＜0.05).In group A,there was no difference in the fluctuation value of blood glucose at different levels of surgery(P＞0.05).In group B,the fluctuation value of blood glucose in patients with secondary and tertiary surgery was higher than that in quaternary surgery(P＜0.05).In group C,the fluctuation value of blood glucose in patients with tertiary and quaternary surgery was higher than that in patients with secondary surgery(P＜0.05).Conclusions:For ophthalmic surgery patients with T2DM,the postoperative blood glucose values of patients undergoing secondary and tertiary surgery generally show a downward trend,while the postoperative blood glucose value of patients undergoing quaternary surgery generally shows an upward trend.It is suggested that clinical workers should actively manage the perioperative blood glucose of patients with high-level surgery.