Rapid and ultrasensitive detection of pathogen-associated biomarkers is vital for the early diagnosis and therapy of bacterial infections. Herein, we developed a close-packed and ordered Au@AgPt array coupled with a cascade triggering strategy for surface-enhanced Raman scattering (SERS) and colorimetric identification of the Staphylococcus aureus biomarker micrococcal nuclease (MNase) in serum samples. The trimetallic Au@AgPt nanozymes can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) molecules to SERS-enhanced oxidized TMB (oxTMB), accompanied by the color change from colorless to blue. In the presence of S. aureus, the secreted MNase preferentially cut the nucleobase AT-rich regions of DNA sequences on magnetic beads (MBs) to release alkaline phosphatase (ALP), which subsequently mediated the oxTMB reduction for inducing the colorimetric/SERS signal fade away. Using this "on-to-off" triggering strategy, the target S. aureus can be recorded in a wide linear range with a limit of detection of 38 CFU/mL in the colorimetric mode and 6 CFU/mL in the SERS mode. Meanwhile, the MNase-mediated strategy characterized by high specificity and sensitivity successfully discriminated between patients with sepsis (n = 7) and healthy participants (n = 3), as well as monitored the prognostic progression of the disease (n = 2). Overall, benefiting from highly active and dense "hot spot" substrate, MNase-mediated cascade response strategy, and colorimetric/SERS dual-signal output, this methodology will offer a promising avenue for the early diagnosis of S. aureus infection.

Objective To investigate the application effect of APP in continuing care of discharged patients with condylar fracture after operation. Methods By convenience sampling,from September 2016 to March 2017 in a ward of oral and maxillofacial surgery and received surgical treatment of condylar fracture patients 81 cases, randomly divided into observation group (41 cases) and control group (40 cases).On the basis of routine health education,the observation group carried out continuous care by APP, while the control group received routine health education. The opening type, opening degree, occlusion relation and temporomandibular joint pain in two groups were compared at 6 months after discharge. Results The results of 6 months, the patients in the observation group were open type, occlusion and TMJ pain were better than the control group, the opening degree of the observation group was (35.51 ± 4.34) mm, control group was (31.28 ± 4.57) mm, the difference between the two groups was statistically significant(t=-4.28,P<0.05).There was no statistical difference between the two groups of occlusion and temporomandibular joint pain (P>0.05). Conclusions APP can improve the open type, opening and occlusal relation and temporomandibular joint pain in patients with condylar fracture after operation.

Objective To prepare and characterize docetaxel incorporated phosphatidylcholine -sodium desoxycholate mixed micelles (DOX EPC/SDC MMs).Methods Docetaxel mixed micelles (DOX MMs) were prepared using the co-precipitation method .An optimized formulation was obtained by single factor method , which was used to study the effect of several factors on water solubility of DOX in MMs, including EPC/total(EPC+SDC)concentration, total(EPC+SDC) concentration, the pH and ionic strength of the hydrated solution .Then, the obtained DOX MMs were characterized by investigating particle size distribution and morphology , and by conducting dilute experiments to examin micelle stability . Results The EPC/total ( EPC+SDC) concentration ratio was 0.4.The total supplementary material concentration was 2.5%.Pure water was used to disperse the film .The drug loaded MMs had an average size of 18.56 nm and average zeta potential of -24.3 mV.A roundlike shape was observed under a transmission electron microscope ( TEM).Conclusion DOX EPC/SDC MMs, prepared by a simple and convenient method , can enhance water solubility , is physically compatible with injection solutions and is expected to become a new drug delivery system .

Objective To understand the current situation of mastering the knowledge of nurses' professional relevant laws and regulations for nursing students.Methods Two hundred and sixty-two nursing students were surveyed by the self-designed questionnaire.Results The nursing students lacked a full understanding of the knowledge of nurses' professional relevant laws and regulations.The accuracy of mastering the knowledge of the laws and regulations which were closely related to clinic including the period of validity of the registration of practicing nurses,the apphcation time after revoking the practicing certificate and the evaluation of medical accidents in undergraduates nurse students were respectively 26.46％,24.43％,27.23％,and the junior college nursing students were 18.18％,30.36％,24.24％,and the differences were not statistically significant (x2 =2.509 5,1.145 9,0.297 3,respectively; P ＞ 0.05).Most nursing students had positive attitude about receiving the training of the knowledge of laws and regulations in the process of the practice,and 204 (77.90％) respondents thought it was very necessary to learn and master the knowledge of nurses' professional relevant laws and regulations.Condusions Both the schools and hospitals should pay attention to the training of the knowledge of nurses' professional relevant laws and regulations,and cultivate their legal consciousness.The nursing students should consciously learn about the relevant knowledge to prepare for the clinical work in the future.