Objective:To investigate the iodine nutrition status of pregnant women in Zhejiang Province, explore the impact of salt industry system reform on iodine nutrition level of pregnant women, and provide scientific basis for prevention and treatment of iodine deficiency disorders.Methods:From 2016 to 2021, a multi-stage stratified sampling method was used to select 100 pregnant women from 90 counties (cities, districts) in Zhejiang Province each year as survey subjects. Salt samples were collected from pregnant women's families, and one random urine sample was taken for salt iodine and urinary iodine level testing, respectively.Results:A total of 56 581 samples of household edible salt were collected from pregnant women, with a median salt iodine level of 23.20 mg/kg. Among them, 7 961 were non iodized salt, 45 803 were qualified iodized salt, and 2 817 were unqualified iodized salt. The iodized salt coverage rate was 85.93% (48 620/56 581), and the qualified iodized salt consumption rate was 80.95% (45 803/56 581). The proportion of non iodized salt increased from 10.05% (897/8 928) in 2016 to 15.09% (1 461/9 679) in 2021 (χ 2trend = 95.16, P < 0.001). A total of 56 581 urine samples were collected from pregnant women, with a median urinary iodine level of 130.50 μg/L. Among them, the proportions of urinary iodine levels < 150, 150 - 249, 250 - 499, and ≥500 μg/L were 58.32% (32 996/56 581), 27.24% (15 410/56 581), 12.24% (6 926/56 581), and 2.21% (1 249/56 581), respectively. The median urinary iodine level of pregnant women in inland areas was significantly higher than that in coastal areas ( Z = 19.15, P < 0.001). Furthermore, urinary iodine levels exhibited a non-linear decline as age increased (χ 2regression = 12.65, P < 0.001; χ 2partial = 22.65, P < 0.001) and as pregnancy progressed (χ 2regression = 37.28, P < 0.001; χ 2partial = 18.89, P < 0.001). Conclusions:The overall iodine nutrition status of pregnant women in Zhejiang Province is in a state of iodine deficiency (< 150 μg/L), and there is a greater risk in coastal areas compared to inland areas. However, in the context of the reform of the salt industry system, it is still necessary to strengthen the quality supervision of iodized salt, provide scientific iodine supplementation education, promote specialized iodized salt for pregnant women, and strengthen interventions for prevention and control of iodine deficiency disorders.

Objective:To investigate the iodine nutrition status of pregnant women in Zhejiang Province, explore the impact of salt industry system reform on iodine nutrition level of pregnant women, and provide scientific basis for prevention and treatment of iodine deficiency disorders.Methods:From 2016 to 2021, a multi-stage stratified sampling method was used to select 100 pregnant women from 90 counties (cities, districts) in Zhejiang Province each year as survey subjects. Salt samples were collected from pregnant women's families, and one random urine sample was taken for salt iodine and urinary iodine level testing, respectively.Results:A total of 56 581 samples of household edible salt were collected from pregnant women, with a median salt iodine level of 23.20 mg/kg. Among them, 7 961 were non iodized salt, 45 803 were qualified iodized salt, and 2 817 were unqualified iodized salt. The iodized salt coverage rate was 85.93% (48 620/56 581), and the qualified iodized salt consumption rate was 80.95% (45 803/56 581). The proportion of non iodized salt increased from 10.05% (897/8 928) in 2016 to 15.09% (1 461/9 679) in 2021 (χ 2trend = 95.16, P < 0.001). A total of 56 581 urine samples were collected from pregnant women, with a median urinary iodine level of 130.50 μg/L. Among them, the proportions of urinary iodine levels < 150, 150 - 249, 250 - 499, and ≥500 μg/L were 58.32% (32 996/56 581), 27.24% (15 410/56 581), 12.24% (6 926/56 581), and 2.21% (1 249/56 581), respectively. The median urinary iodine level of pregnant women in inland areas was significantly higher than that in coastal areas ( Z = 19.15, P < 0.001). Furthermore, urinary iodine levels exhibited a non-linear decline as age increased (χ 2regression = 12.65, P < 0.001; χ 2partial = 22.65, P < 0.001) and as pregnancy progressed (χ 2regression = 37.28, P < 0.001; χ 2partial = 18.89, P < 0.001). Conclusions:The overall iodine nutrition status of pregnant women in Zhejiang Province is in a state of iodine deficiency (< 150 μg/L), and there is a greater risk in coastal areas compared to inland areas. However, in the context of the reform of the salt industry system, it is still necessary to strengthen the quality supervision of iodized salt, provide scientific iodine supplementation education, promote specialized iodized salt for pregnant women, and strengthen interventions for prevention and control of iodine deficiency disorders.

This study aimed to identify,characterize,and functionally analyze the CYP450 gene fam-ily within Hyalomma asiaticum unfed nymphs(HasiUN)under regular physiological conditions,laying the groundwork for further study into the biosynthesis and metabolism of exogenous sub-stances associated with the CYP450 gene in HasiUN.Through our methods,we identified and characterized HasiUNCYP450 genes.We then annotated them using the gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG).This was achieved with the help of bioinforma-tics,based on the transcriptome of 2-week-old HasiUN.Further on,we conducted a preliminary a-nalysis of tissue expression,distribution,and potential function with qRT-PCR and network inter-action predictions.Our analyses showed that 87 HasiUN CYP450 genes,ranging in size from 1089 to 1692 bp,encoded predominantly basic,hydrophilic,and unstable protein sequences.These se-quences had nearly identical numbers of transmembrane regions,both with and without a signal peptide.Phylogenetic and domain analyses indicated that HasiUN CYP450,having a complete CYP450 domain,were distributed among various clans,with most members found in clan 3.CYP20 clans,present in 13 species from 2 families and 7 genera,required either one-host or three-hosts to undergo their full life cycle.MEME analysis results hint at different clans having variations in the amount and combination of motifs.Expression level analysis showed that HasiUN CYP450 was expressed at different levels,with the highest distribution of normally expressed genes in clan 3 and low expression of all mito clan genes.Functional annotation revealed HasiUN CYP450 key as-sociation with biological processes,metabolism,and organismal systems according to the GO and KEGG annotations.Distribution results showed that HasiUN CYP20a1 was expressed in specific tissues,including salivary glands and ovaries.Functional analysis points to CYP20a1's role in UV-induced toxic metabolism,endocrine disruptor metabolism,and hazardous substance metabolism in the nervous system.Our research presents the first analysis of HasiUN CYP450 gene's biological characteristics and expression pattern under normal physiological conditions.Additionally,investi-gations into the distribution and function of a new category,HasiUN CYP20a1,were embarked upon.These findings establish a theoretical foundation for continued functional study of HasiUN CYP45 gene.

Objective To investigate the characteristics and development of stops consonant production in Mandarin-speaking prelingually deaf children after cochlear implantation.Methods Thirty prelingually deaf children with a mean age of 4.37 years(SD=1.57)who received unilateral cochlear implantation(CI)under the age of 3,and thirty age-matched normal hearing(NH)children with a mean age of 4.02 years(SD=0.67)participated in the test.Word lists developed by Institute of Linguistics,Chinese Academy of Social Sciences were selected.The accu-racy of the stops(/b/,/d/,/p/,/t/,/k/,/g/)production performance and error types were analyzed.Results① The accuracy of children's stops output in the CI group was significantly lower than that in the NH group.The correct rate of each stop in the CI group was/b/＞/d/＞/p/＞/t/＞/g/＞/k/,and that in the NH group was/b/＞/p/＞/d/＞/t/＞/g/＞/k/.② The error type of CI children's stops output was similar to the NH group,with the main error being substitution error.The most common errors involved substitutions within the same place of ar-ticulation,and the more difficult sounds being replaced by the easier ones.③ With the increase in physiological age and hearing age,the total accuracy of stops production of CI children increased.The greatest progress was made 3 years after the operation,but the accuracy still did not reach 100％.There was no significantly correlation with im-plantation age(P＞0.05).Conclusion The accuracy of stops production in the CI group was lower than that in the NH group.With the increase of hearing and physiological age,stops production ability improved gradually and de-veloped rapidly within 3 years after the operation.

With the advancement of China's healthcare reform,enhancing the capacity of primary healthcare services has become a pivotal task.Colorectal cancer,one of the most prevalent malignancies in China,highlights the critical importance of early screening and diagnosis to improve patient survival rates.This study,guided by the principles of Party building and Xi Jinping Thought on Socialism with Chinese Characteristics,examines the implementation and outcomes of a rural outreach program focused on colorectal cancer screening and diagnostic technologies.By promoting the dissemination of colorectal cancer screening initiatives,the paper aims to provide empirical evidence to support the deepening of primary-care services,foster high-quality ad-vancement of grassroots health services,and align with the national Healthy China Initiative,thereby more effectively safeguarding population health.

To explore the effects of different exercise prescriptions on glycemic metabolism in East Asian patients with type 2 diabetes mellitus (T2DM) and to compare the differences in the impact of population characteristics and exercise components on glycemic metabolism.

Objective To investigate the reliability and validity of the Chinese version of speech,spatial and qualities of hearing scale(SSQ).Methods The SSQ was translated into Chinese using the translation and back-translation method.Self-assessment with the scale was conducted for 50 adults with normal hearing and 28 adults with hearing impairment who had bilateral hearing aids fitted.Two weeks after the initial assessment,10 individuals with bilateral hearing aids fitted were randomly selected to be re-assessed using the same scale.The Cronbach's αcoefficients of the scores of each dimension and the total score of the scale was used to evaluate the internal consis-tency of the scale,and the correlation coefficient of the results of the two assessments was used to test the test-retest reliability of the scale.The expert evaluation method was adopted to test the content validity of the scale,and the correlation coefficients of the scores of each dimension and the total score were used to test the construct validity of the scale.The score differences of each item and each dimension between adults with normal hearing and adults with bilateral hearing aids fitted were compared to test the discriminant validity.Results Valid responses were obtained from all 78 participants.① The test-retest reliability coefficients of the total scale and the three dimensions were all greater than 0.750(P＜0.01),indicating excellent test-retest reliability.The Cronbach's α coefficients of the total scale and the three dimensions were all greater than 0.700,suggesting excellent internal consistency.② All four ex-perts indicated that the content of each item in the three dimensions of the SSQ scale was in line with the measure-ment requirements,showing excellent content validity.The correlation coefficients between the scores of the three dimensions and the total scale were between 0.809 and 0.890,indicating a high degree of correlation.There were statistically significant differences in the scores of the three dimensions between the normal group and the group with bilateral hearing aids fitted(P＜0.05),and the overall discriminant validity of the scale was excellent.Conclusion The Chinese version of the SSQ has good reliability and validity,and can be used as a subjective auditory assessment in clinical practice.

With the advancement of China's healthcare reform,enhancing the capacity of primary healthcare services has become a pivotal task.Colorectal cancer,one of the most prevalent malignancies in China,highlights the critical importance of early screening and diagnosis to improve patient survival rates.This study,guided by the principles of Party building and Xi Jinping Thought on Socialism with Chinese Characteristics,examines the implementation and outcomes of a rural outreach program focused on colorectal cancer screening and diagnostic technologies.By promoting the dissemination of colorectal cancer screening initiatives,the paper aims to provide empirical evidence to support the deepening of primary-care services,foster high-quality ad-vancement of grassroots health services,and align with the national Healthy China Initiative,thereby more effectively safeguarding population health.

This study aimed to identify,characterize,and functionally analyze the CYP450 gene fam-ily within Hyalomma asiaticum unfed nymphs(HasiUN)under regular physiological conditions,laying the groundwork for further study into the biosynthesis and metabolism of exogenous sub-stances associated with the CYP450 gene in HasiUN.Through our methods,we identified and characterized HasiUNCYP450 genes.We then annotated them using the gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG).This was achieved with the help of bioinforma-tics,based on the transcriptome of 2-week-old HasiUN.Further on,we conducted a preliminary a-nalysis of tissue expression,distribution,and potential function with qRT-PCR and network inter-action predictions.Our analyses showed that 87 HasiUN CYP450 genes,ranging in size from 1089 to 1692 bp,encoded predominantly basic,hydrophilic,and unstable protein sequences.These se-quences had nearly identical numbers of transmembrane regions,both with and without a signal peptide.Phylogenetic and domain analyses indicated that HasiUN CYP450,having a complete CYP450 domain,were distributed among various clans,with most members found in clan 3.CYP20 clans,present in 13 species from 2 families and 7 genera,required either one-host or three-hosts to undergo their full life cycle.MEME analysis results hint at different clans having variations in the amount and combination of motifs.Expression level analysis showed that HasiUN CYP450 was expressed at different levels,with the highest distribution of normally expressed genes in clan 3 and low expression of all mito clan genes.Functional annotation revealed HasiUN CYP450 key as-sociation with biological processes,metabolism,and organismal systems according to the GO and KEGG annotations.Distribution results showed that HasiUN CYP20a1 was expressed in specific tissues,including salivary glands and ovaries.Functional analysis points to CYP20a1's role in UV-induced toxic metabolism,endocrine disruptor metabolism,and hazardous substance metabolism in the nervous system.Our research presents the first analysis of HasiUN CYP450 gene's biological characteristics and expression pattern under normal physiological conditions.Additionally,investi-gations into the distribution and function of a new category,HasiUN CYP20a1,were embarked upon.These findings establish a theoretical foundation for continued functional study of HasiUN CYP45 gene.

Objective To investigate the characteristics and development of stops consonant production in Mandarin-speaking prelingually deaf children after cochlear implantation.Methods Thirty prelingually deaf children with a mean age of 4.37 years(SD=1.57)who received unilateral cochlear implantation(CI)under the age of 3,and thirty age-matched normal hearing(NH)children with a mean age of 4.02 years(SD=0.67)participated in the test.Word lists developed by Institute of Linguistics,Chinese Academy of Social Sciences were selected.The accu-racy of the stops(/b/,/d/,/p/,/t/,/k/,/g/)production performance and error types were analyzed.Results① The accuracy of children's stops output in the CI group was significantly lower than that in the NH group.The correct rate of each stop in the CI group was/b/＞/d/＞/p/＞/t/＞/g/＞/k/,and that in the NH group was/b/＞/p/＞/d/＞/t/＞/g/＞/k/.② The error type of CI children's stops output was similar to the NH group,with the main error being substitution error.The most common errors involved substitutions within the same place of ar-ticulation,and the more difficult sounds being replaced by the easier ones.③ With the increase in physiological age and hearing age,the total accuracy of stops production of CI children increased.The greatest progress was made 3 years after the operation,but the accuracy still did not reach 100％.There was no significantly correlation with im-plantation age(P＞0.05).Conclusion The accuracy of stops production in the CI group was lower than that in the NH group.With the increase of hearing and physiological age,stops production ability improved gradually and de-veloped rapidly within 3 years after the operation.