Objective This study designed to evaluate the impact of interferon α-2b vaginal effervescent tablets in combination with CO2 cryotherapy on the immune markers and inflammation levels in patients with CIN and concurrent HPV infection,and to explore its potential effects in disease treatment.Methods A total of 124 patients with CIN and HPV infection,admitted to our hospital from 1st Jan 2023 to 18th Feb 2024,were selected and randomly divided into an observation group(Group B)and a control group(Group A),with 62 patients in each.The control group received only interferon α-2b vaginal effervescent tablets,while the observation group also underwent CO2 cryotherapy.The immune cell counts,cytokine levels,inflammation markers,and HPV-DNA quantification were as-sessed both before and after treatment.Results The observation group showed significant increase in immune cell counts(CD4+/CD8+)and cytokine levels(IFN-γ and TNF-α)after the treatment.Moreover,reductions in inflammation markers(CRP,IL-6 and IL-8)and a higher HPV clearance rate(67.74％)compared to the control group(45.16％),with a significantly lower recurrence rate(6.45％vs 16.12％),were observed.Conclusion The combination of interferon α-2b and CO2 cryotherapy have significantly in-creased the immune markers and reduced inflammation levels in patients with CIN and HPV,and effectively reduced the HPV recurrence rate.This combined treatment strategy presents an effective strategy for the management of precancerous cervical lesions.

BACKGROUND:Studies have shown a significant association between members of the insulin-like growth factor family and the occurrence of inflammatory arthritis,but the causal relationship has not been accurately characterized.OBJECTIVE:To explore the potential association between members of the insulin-like growth factor family and the occurrence and development of ankylosing spondylitis,rheumatoid arthritis,and psoriatic arthritis.METHODS:Genetic instrumental variables associated with 14 discrete members of the insulin-like growth factor family,primarily derived from the expansive genomic database of a genome-wide association study,were used.The pertinent summary statistics for ankylosing spondylitis,rheumatoid arthritis,and psoriatic arthritis were meticulously procured from the FinnGen Consortium's extensive dataset.Our primary analytical methodology was anchored in the inverse-variance weighted approach,which is recognized for its robustness in Mendelian randomization studies.To augment the credibility and broader applicability of the findings,an array of complementary analyses were performed.These encompassed the weighted-median method,which mitigates the influence of potential outliers;the MR-Egger regression,a tool for assessing directional pleiotropy;the weighted mode and simple mode approaches,which provide alternative estimates of the causal effect;the MR pleiotropy residual sum and outlier test,designed to identify and correct for horizontal pleiotropy;Cochran's Q statistic test,which evaluates the heterogeneity of the effect estimates;and the MR-Egger intercept analysis,a diagnostic for detecting and adjusting the impact of pleiotropic relationships.RESULTS AND CONCLUSION:We identified four distinct causal associations:CYR61 protein was negatively correlated with ankylosing spondylitis(odd ratios[OR]:0.919,95％confidence interval[CI]:0.848-0.997,P=0.042)and rheumatoid arthritis(OR:0.946,95％CI:0.908-0.987,P=0.011);IGF-Ⅱ receptor was negatively correlated with ankylosing spondylitis(OR:0.909,95％CI:0.835-0.990,P=0.029);IGFBP-7 was positively correlated with psoriatic arthritis(OR:1.104,95％CI:1.002-1.218,P=0.046).The results of sensitivity analyses were consistent(P＜0.05).This rigorous analytical approach has yielded evidence suggestive of a potential causal nexus between a constellation of insulin growth factor family members and the risk of inflammatory arthritis.These findings underscore the necessity for further research to delineate the precise mechanisms by which insulin-like growth factor family members influence the developmental trajectories of ankylosing spondylitis,rheumatoid arthritis,and psoriatic arthritis,thereby providing evidence for developing targeted interventions.In addition,by drawing on international research experience,future biomedical research in China should further focus on the genetic mechanisms of inflammatory and immune-related diseases,promote clinical translation and the development of precision medicine,and provide more efficient and personalized treatment plans for Chinese patients.

BACKGROUND:Studies have shown a significant association between members of the insulin-like growth factor family and the occurrence of inflammatory arthritis,but the causal relationship has not been accurately characterized.OBJECTIVE:To explore the potential association between members of the insulin-like growth factor family and the occurrence and development of ankylosing spondylitis,rheumatoid arthritis,and psoriatic arthritis.METHODS:Genetic instrumental variables associated with 14 discrete members of the insulin-like growth factor family,primarily derived from the expansive genomic database of a genome-wide association study,were used.The pertinent summary statistics for ankylosing spondylitis,rheumatoid arthritis,and psoriatic arthritis were meticulously procured from the FinnGen Consortium's extensive dataset.Our primary analytical methodology was anchored in the inverse-variance weighted approach,which is recognized for its robustness in Mendelian randomization studies.To augment the credibility and broader applicability of the findings,an array of complementary analyses were performed.These encompassed the weighted-median method,which mitigates the influence of potential outliers;the MR-Egger regression,a tool for assessing directional pleiotropy;the weighted mode and simple mode approaches,which provide alternative estimates of the causal effect;the MR pleiotropy residual sum and outlier test,designed to identify and correct for horizontal pleiotropy;Cochran's Q statistic test,which evaluates the heterogeneity of the effect estimates;and the MR-Egger intercept analysis,a diagnostic for detecting and adjusting the impact of pleiotropic relationships.RESULTS AND CONCLUSION:We identified four distinct causal associations:CYR61 protein was negatively correlated with ankylosing spondylitis(odd ratios[OR]:0.919,95％confidence interval[CI]:0.848-0.997,P=0.042)and rheumatoid arthritis(OR:0.946,95％CI:0.908-0.987,P=0.011);IGF-Ⅱ receptor was negatively correlated with ankylosing spondylitis(OR:0.909,95％CI:0.835-0.990,P=0.029);IGFBP-7 was positively correlated with psoriatic arthritis(OR:1.104,95％CI:1.002-1.218,P=0.046).The results of sensitivity analyses were consistent(P＜0.05).This rigorous analytical approach has yielded evidence suggestive of a potential causal nexus between a constellation of insulin growth factor family members and the risk of inflammatory arthritis.These findings underscore the necessity for further research to delineate the precise mechanisms by which insulin-like growth factor family members influence the developmental trajectories of ankylosing spondylitis,rheumatoid arthritis,and psoriatic arthritis,thereby providing evidence for developing targeted interventions.In addition,by drawing on international research experience,future biomedical research in China should further focus on the genetic mechanisms of inflammatory and immune-related diseases,promote clinical translation and the development of precision medicine,and provide more efficient and personalized treatment plans for Chinese patients.

Objective This study designed to evaluate the impact of interferon α-2b vaginal effervescent tablets in combination with CO2 cryotherapy on the immune markers and inflammation levels in patients with CIN and concurrent HPV infection,and to explore its potential effects in disease treatment.Methods A total of 124 patients with CIN and HPV infection,admitted to our hospital from 1st Jan 2023 to 18th Feb 2024,were selected and randomly divided into an observation group(Group B)and a control group(Group A),with 62 patients in each.The control group received only interferon α-2b vaginal effervescent tablets,while the observation group also underwent CO2 cryotherapy.The immune cell counts,cytokine levels,inflammation markers,and HPV-DNA quantification were as-sessed both before and after treatment.Results The observation group showed significant increase in immune cell counts(CD4+/CD8+)and cytokine levels(IFN-γ and TNF-α)after the treatment.Moreover,reductions in inflammation markers(CRP,IL-6 and IL-8)and a higher HPV clearance rate(67.74％)compared to the control group(45.16％),with a significantly lower recurrence rate(6.45％vs 16.12％),were observed.Conclusion The combination of interferon α-2b and CO2 cryotherapy have significantly in-creased the immune markers and reduced inflammation levels in patients with CIN and HPV,and effectively reduced the HPV recurrence rate.This combined treatment strategy presents an effective strategy for the management of precancerous cervical lesions.

OBJECTIVE To sum marize the pro cedure and informatization construction of centralized volume-based procurement（VBP）in our hospital ，in order to give references for normal development of centralized VBP. METHODS The standardized workflow system was established ，including using flow chart method to establish standardized workflow ，carrying out procedure training and inspection of procedure implementation ， and continuously conducting procedure optimization. The information system was developed for the task links that needed a lot of calculation to improve the automation level of information processing. RESULTS & CONCLUSIONS Eight specific work procedures were established in our hospital ，including the work procedures of submitting the demand data of centralized VBP and the implementation of centralized VBP ，and has taken measures such as procedure training ，establishing supervision and inspection system and using auxiliary means to promote the implementation of the procedure ，so as to optimize the procedure and work form. An informatization platform for the clinical task allocation of the agreed purchase quantity of centralized VBP and a supervision platform for the daily use of VBP were also established in our hospital，then the two tasks with a large amount of calculation could be finished. Standardized workflow system and informatization platform construction has improved the operation and supervision efficiency of centralized VBP in our hospital ，ensured the completion of centralized purchase tasks and saved human resources ，which has a certain promotion value.

Background: Bovine papillomatosis is a type of proliferative tumor disease of skin and mucosae caused by bovine papillomavirus (BPV). As a transboundary and emerging disease in cattle, it poses a potential threat to the dairy industry. Objectives: The aim of this study is to detect and clarify the genetic diversity of BPV circulating in dairy cows in Xinjiang, China. Methods: 122 papilloma skin lesions from 8 intensive dairy farms located in different regions of Xinjiang, China were detected by polymerase chain reaction. The genetic evolution relationships of various types of BPVs were analyzed by examining this phylogenetic tree. Results: Ten genotypes of BPV (BPV1, BPV2, BPV3, BPV6, BPV7, BPV8, BPV10, BPV11, BPV13, and BPV14) were detected and identified in dairy cows. These were the first reported detections of BPV13 and BPV14 in Xinjiang, Mixed infections were detected, and there were geographical differences in the distribution of the BPV genotypes. Notably, the BPV infection rate among young cattle (< 1-year-old) developed from the same supply of frozen sperm was higher than that of the other young cows naturally raised under the same environmental conditions. Conclusions Genotyping based on the L1 gene of BPV showed that BPVs circulating in Xinjiang China displayed substantial genetic diversity. This study provided valuable data at the molecular epidemiology level, which is conducive to developing deep insights into the genetic diversity and pathogenic characteristics of BPVs in dairy cows.

Centralized drug procurement in large quantities is a major step to deepen the medical and healthcare system reform, to improve the mechanism of drug price formation, and to give full play to the role of medical insurance in guiding drug prices in China. Combined with the practice of centralized drug purchasing in public hospitals, the authors sorted out the practical problems and causes from four aspects which affected the implementation of centralized drug purchasing policy in public hospitals: selection on centrally purchased drugs, procurement and supply, clinical use, and hospital financial operation. On this basis, suggestions were put forward to provide reference for the normalization of centralized drug procurement work, such as coordinating national and regional drug collection policies, reasonably setting distribution costs, extending the agreed procurement period, and carrying out special evaluation for drug collection.

Background: Bovine papillomatosis is a type of proliferative tumor disease of skin and mucosae caused by bovine papillomavirus (BPV). As a transboundary and emerging disease in cattle, it poses a potential threat to the dairy industry. Objectives: The aim of this study is to detect and clarify the genetic diversity of BPV circulating in dairy cows in Xinjiang, China. Methods: 122 papilloma skin lesions from 8 intensive dairy farms located in different regions of Xinjiang, China were detected by polymerase chain reaction. The genetic evolution relationships of various types of BPVs were analyzed by examining this phylogenetic tree. Results: Ten genotypes of BPV (BPV1, BPV2, BPV3, BPV6, BPV7, BPV8, BPV10, BPV11, BPV13, and BPV14) were detected and identified in dairy cows. These were the first reported detections of BPV13 and BPV14 in Xinjiang, Mixed infections were detected, and there were geographical differences in the distribution of the BPV genotypes. Notably, the BPV infection rate among young cattle (< 1-year-old) developed from the same supply of frozen sperm was higher than that of the other young cows naturally raised under the same environmental conditions. Conclusions Genotyping based on the L1 gene of BPV showed that BPVs circulating in Xinjiang China displayed substantial genetic diversity. This study provided valuable data at the molecular epidemiology level, which is conducive to developing deep insights into the genetic diversity and pathogenic characteristics of BPVs in dairy cows.

Objective To study the effect of the combined psychological intervention of ambroxol on the patients with lung cancer after thoracoscopic lobectomy. Methods January 2015 to December 2016, Beijing Jian Gong Hospital under thoracoscope lung resection of 64 cases of patients with lung cancer, the control group treated with saline 600 mL/d, experimental group implementation of ammonia bromine line on postoperative 3 days for the patient on the day of surgery until given intravenous 600 mL/d hydrochloric acid ammonia bromine injection combined psychological intervention treatment. Results There were no deaths in the perioperative period, but the lung complications in the experimental group were significantly lower than those in the control group. There were significant differences in mechanical ventilation, ICU time, postoperative hospital stay, and treatment cost after surgery (P < 0.05). Conclusion For patients with lung cancer in perioperative implementation under the thoracoscope lung resection and intravenous hydrochloric acid ammonia bromine joint line comments fluid psychological intervention treatment, effectively improve the patients' lung function and significantly lower the incidence of complications, shorten the patient's hospital stay, reduce the economic burden, and can be applied clinically.

Objective To investigate the effects of p38 MAPK inhibitor on glutamate (Glu), glutamine (Gln), taurine amino acid (Tau), glycine (Gly) and gamma aminobutyric acid (GABA) in hippocampus of rats with vascular dementia (VD).Methods Twenty-four healthy male SD rats were randomly divided into sham operation group, VD model group and inhibitor group. VD model was established by permanent ligation of bilateral carotid artery method, and the sham operation group stripping bilateral carotid artery but not ligation. Rats of the inhibitor group were injected p38 MAPK inhibitor SB202190 after the establishment of VD model. Morris water maze was used to evaluate the learning and memory function of rats. The samples of DG region of hippocampus were collected by microdialysis, and the contents of amino acids were detected. Results The learning and memory abilities were significantly better in sham operation group and p38 MAPK inhibitor group than those of VD model group. The latency time was significantly shorter in p38 MAPK group than that of VD model group. The time of locating the platform quadrant and the number of crossing the original platform were significantly higher in sham operation group and p38 MAPK inhibitor group than those of VD model group (P <0.05). Compared with sham group, the levels of Glu, Gln and Tau were significantly lower, and Gly and GABA were significantly higher, in VD group and p38 MAPK inhibitor group (P<0.05). Compared with VD group, Glu, Gln and Tau were significantly increased, Gly and GABA were significantly decreased in p38 MAPK inhibitor group (P<0.05). Conclusion p38 MAPK inhibitor has protective effect on VD hippocampal injury, which may be related to its ability to inhibit VD-induced abnormal secretion of amino acids and regulate the secretion of various amino acids.