Objective To explore the clinical effect of quality control circle(QCC)activities on the prevention of postoperative deep vein thrombosis(DVT)in inpatients in military hospitals.Methods A total of 318 patients who were diagnosed and treated in The First Affiliated Hospital of Air Force Medical University from January to December 2021 and 40 medical staff were enrolled in this study.Routine care was performed in 158 patients from January to June 2021,and QCC care was implemented in 160 patients from July to December 2021.The awareness of DVT prevention in medical staff and patients(or their famiy members)before and after QCC activities,lower limb DVT preventive measures taken by medical staff,and the occurrence of DVT were compared.Results The scores of the cause,clinical manifestations,nursing measures and preventive measures of DVT after QCC activities were significantly higher than those before QCC activities in both medical staff and patients(or their families)(P<0.05).The overall implementation rate of preventing lower limb DVT after QCC activities was significantly higher than that before QCC activities(94.14%vs.46.20%,P<0.05).The incidence of DVT after QCC activities was significantly lower than that before QCC activities(0.62%vs.5.06%,P<0.05).Conclusion Implementing QCC activities can improve the cognitive ability of military medical staff and patients(or family members)in DVT prevention,increase the implementation rate of DVT prevention measures,and reduce the incidence of DVT.

Objective:To explore the early diagnostic value of combined detection of serum chitinase protein 40 (YKL-40), galectin-3 (Gal-3), and regulated upon activation normal T cell expressed and secreted (RANTES) for refractory mycoplasma pneumoniae pneumonia (RMPP) in children.Methods:One hundred and twenty-six children with RMPP (RMPP group), 126 children with global mycoplasma pneumoniae pneumonia (GMPP) (GMPP group), and 126 healthy children who underwent physical check up (control group) in the Affiliated Hospital of Jining Medical College from June 2022 to June 2024 were retrospectively selected. The serum levels of YKL-40, Gal-3 and RANTES were compared. Multivariate Logistic regression was used to analyze the independent influencing factors of RMPP. Receiver operating characteristic (ROC) curve was used to analyze the diagnostic value of serum YKL-40, Gal-3 and RANTES levels for RMPP.Results:Compared with the control group, the serum YKL-40, Gal-3 and RANTES levels in the RMPP and GMPP groups were obviously higher: (42.19 ± 4.94) and (37.68 ± 4.25) μg/L vs. (26.73 ± 3.31) μg/L, (12.24 ± 2.89) and (8.87 ± 2.56) ng/L vs. (3.92 ± 1.27) ng/L, (33.82 ± 3.86) and (29.28 ± 3.72) μg/L vs. (21.34 ± 2.79) μg/L, with statistical significance ( P<0.05). Compared with the GMPP group, the serum YKL-40, Gal-3 and RANTES levels in the RMPP group were obviously higher ( P<0.05). Compared with the GMPP group, there was no statistically obvious difference in age, gender, body weight, duration of fever and atelectasis in the RMPP group ( P>0.05), however, the levels of C-reactive protein (CRP), procalcitonin (PCT), and the proportions of pleural effusion and lung consolidation were obviously higher in the RMPP group: (22.45 ± 4.21) mg/L vs. (18.69 ± 3.56) mg/L, (0.18 ± 0.04) μg/L vs. (0.15 ± 0.03) μg/L, 31.75% (40/126) vs. 17.46% (22/126), 38.89% (49/126) vs. 25.40% (32/126), P<0.05. CRP, PCT, pleural effusion, pulmonary consolidation, YKL-40, Gal-3 and RANTES were all independent influencing factors of RMPP ( P<0.05). The areas under the curve (AUC) of serum YKL-40, Gal-3 and RANTES levels for diagnosing RMPP in children were 0.769, 0.833 and 0.825, respectively. The AUC of the combined diagnosis of YKL-40, Gal-3 and RANTES was 0.923, which was obviously higher than that of the single indicator diagnosis ( Z = 5.373, 3.677 and 4.191; P<0.01). The sensitivity of the three combined diagnosis was 73.81%, and the specificity was 92.86%. Conclusions:Serum YKL-40, Gal-3 and RANTES levels are obviously elevated in patients with RMPP, and all of which are influencing factors of RMPP. The combination of the three has high diagnostic value for RMPP.

Objective:To explore the early diagnostic value of combined detection of serum chitinase protein 40 (YKL-40), galectin-3 (Gal-3), and regulated upon activation normal T cell expressed and secreted (RANTES) for refractory mycoplasma pneumoniae pneumonia (RMPP) in children.Methods:One hundred and twenty-six children with RMPP (RMPP group), 126 children with global mycoplasma pneumoniae pneumonia (GMPP) (GMPP group), and 126 healthy children who underwent physical check up (control group) in the Affiliated Hospital of Jining Medical College from June 2022 to June 2024 were retrospectively selected. The serum levels of YKL-40, Gal-3 and RANTES were compared. Multivariate Logistic regression was used to analyze the independent influencing factors of RMPP. Receiver operating characteristic (ROC) curve was used to analyze the diagnostic value of serum YKL-40, Gal-3 and RANTES levels for RMPP.Results:Compared with the control group, the serum YKL-40, Gal-3 and RANTES levels in the RMPP and GMPP groups were obviously higher: (42.19 ± 4.94) and (37.68 ± 4.25) μg/L vs. (26.73 ± 3.31) μg/L, (12.24 ± 2.89) and (8.87 ± 2.56) ng/L vs. (3.92 ± 1.27) ng/L, (33.82 ± 3.86) and (29.28 ± 3.72) μg/L vs. (21.34 ± 2.79) μg/L, with statistical significance ( P<0.05). Compared with the GMPP group, the serum YKL-40, Gal-3 and RANTES levels in the RMPP group were obviously higher ( P<0.05). Compared with the GMPP group, there was no statistically obvious difference in age, gender, body weight, duration of fever and atelectasis in the RMPP group ( P>0.05), however, the levels of C-reactive protein (CRP), procalcitonin (PCT), and the proportions of pleural effusion and lung consolidation were obviously higher in the RMPP group: (22.45 ± 4.21) mg/L vs. (18.69 ± 3.56) mg/L, (0.18 ± 0.04) μg/L vs. (0.15 ± 0.03) μg/L, 31.75% (40/126) vs. 17.46% (22/126), 38.89% (49/126) vs. 25.40% (32/126), P<0.05. CRP, PCT, pleural effusion, pulmonary consolidation, YKL-40, Gal-3 and RANTES were all independent influencing factors of RMPP ( P<0.05). The areas under the curve (AUC) of serum YKL-40, Gal-3 and RANTES levels for diagnosing RMPP in children were 0.769, 0.833 and 0.825, respectively. The AUC of the combined diagnosis of YKL-40, Gal-3 and RANTES was 0.923, which was obviously higher than that of the single indicator diagnosis ( Z = 5.373, 3.677 and 4.191; P<0.01). The sensitivity of the three combined diagnosis was 73.81%, and the specificity was 92.86%. Conclusions:Serum YKL-40, Gal-3 and RANTES levels are obviously elevated in patients with RMPP, and all of which are influencing factors of RMPP. The combination of the three has high diagnostic value for RMPP.

In order to establish a rapid,specific and sensitive detection method for Streptococcus equi subspecies zooepidemicus(SEZ)and to understand the infection status of SEZ in horses ente-ring China,specific primers were designed and synthesized based on the conserved gene comB of standard strain SEZ(ATCC 43079)in this work.Then,the pMD19-T-comB recombinant plasmid was constructed and used as a standard positive template.After that,the fluorescence-based quantitative PCR(qPCR)detection method based on SYBR Green Ⅰ dye was established.Totally,477 equine entry serum samples from 6 countries,including Netherlands,Belgium,Japan,Germa-ny,Argentina and New Zealand,during 2018 to 2023,were randomly selected and detected for SEZ by the qPCR method.Results showed that the established qPCR method had specific amplification for only SEZ,which illustrated a good specificity.Sensitivity test of the method showed that the limited detection amount was 4.58 X101 copies/μL.And the repeatability test showed that the coef-ficient of variation of intra-batch repeatability was less than 0.5％,while the inter-batch repeat-ability was less than 3.0％,which indicated good repeatability and high stability.Retrospective a-nalysis showed that totally 11 of 477 positive samples were detected,with a relatively low positive rate of 2.31％(11/477).Among them,all the 40 samples from Netherlands in 2018 were negative(0/40).In the samples of 2019,one positive was detected from Belgium(1/20),while all other 36 samples which form Japan and Germany were negative.In the samples of 2021,three samples(3/34)from Japan and one sample(1/20)from Argentina were positive,and all the other 40 samples from the Netherlands were negative.In the samples of 2022,76 samples from Netherlands were all negative.While in the 2023,5(5/126)of 126 samples from Netherlands and one(1/88)of 88 from New Zealand were found positive with SEZ.To summarize,The SYBR Green Ⅰ qPCR method for the diagnosis of SEZ was successfully established,and it could provide necessary technical support for the rapid quarantine of China's entry-exit and port departments,as well as the epidemiological investigation of the disease.

Objective:To systematically evaluate the correlation between chronic periodontitis and postmenopausal osteoporosis (PMOP).Methods:Electronic searches were conducted on Cochrane Library, Pubmed, Embase, Ovid, CNKI, CBM, VIP, and WF databases to collect research literature on the correlation between chronic periodontitis and PMOP. The Newcastle Ottawa Scale (NOS) criteria were used to evaluate the quality of the included literature, and RevMan 5.3 software was used for meta-analysis. The outcome measures were clinical attachment loss (CAL), probing depth (PD), plaque index (PI), calculus index (CI), bleeding on probing (BOP), and simplified oral hygiene index (OHI-S).Results:A total of 16 articles were included, with a total of 1587 patients. Compared with the postmenopausal non osteoporosis group, the osteoporosis group showed significant abnormalities in CAL [standardized mean difference (SMD)=1.09, 95% CI: 0.62-1.57, P<0.001], PD(SMD=0.71, 95% CI: 0.28-1.14, P<0.001), PI(SMD=0.43, 95% CI: 0.29-0.56, P<0.001), and OHI-S(SMD=0.28, 95% CI: 0.22-0.35, P<0.001) indicators, as well as in BOP(SMD=0.01, 95% CI: -0.48-0.49, P=0.97) and GI(SMD=0.01, 95% CI: -0.48-0.49, P=0.97). At the level of 0.24 and 95% CI: -0.34 to 0.81, P=0.42, there was no statistically significant difference. Conclusions:Women with PMOP exhibit more significant changes in indicators such as CAL, PD, PI, and OHI-S, suggesting that postmenopausal women with osteoporosis are more likely to suffer from periodontitis.

Objective:To clarify the factors that affect the readmission of diabetes patients, and to provide theoretical basis for nurses to evaluate and identify high-risk groups.Methods:We searched Chinese and English databases such as China National Knowledge Infrastructure, WanFang Data, VIP, PsycINFO, PubMed, Medline, Cochrane Library, Web of Science, SinoMed and so on through computers. The search period was from the establishment of the database to June 2022. Data extraction and quality evaluation were conducted on the included literature, and Meta-analysis was conducted using RevMan 5.3 statistical software.Results:A total of 17 articles were included, including 52 risk factors. The Meta-analysis results showed that female [ OR=0.95, 95% CI (0.93, 0.96) , P<0.000 01] , blood glucose [ OR=1.41, 95% CI (1.16, 1.70) , P=0.000 4] , hospital stay [ OR=1.04, 95% CI (1.00, 1.08) , P=0.04] , disability [ OR=1.58, 95% CI (1.30, 1.92) , P<0.000 01] , retirement [ OR=1.45, 95% CI (1.31, 1.61) , P<0.000 01] , unemployment [ OR=1.35, 95% CI (1.26, 1.45) , P<0.000 01] , insulin use [ OR=1.19, 95% CI (1.14, 1.24) , P<0.000 01] , low blood sodium [ OR=1.19, 95% CI (1.08, 1.30) , P=0.000 3] , hematocrit [ OR=0.90, 95% CI (0.89, 0.92) , P<0.000 01] , discharge without following medical advice [ OR=1.63, 95% CI (1.53, 1.72) , P<0.000 01] , emergency [ OR=1.20, 95% CI (1.18, 1.21) , P<0.000 01] , fluid and electrolyte disorders [ OR=1.15, 95% CI (1.08, 1.22) , P<0.000 01] , heart failure [ OR=1.24, 95% CI (1.15, 1.33) , P<0.000 01] , complications [ OR=1.12, 95% CI (1.00, 1.25) , P<0.05] , renal failure [ OR=1.98, 95% CI (1.79, 2.19) , P<0.000 01] , anemia [ OR=1.25, 95% CI (1.15, 1.36) , P<0.000 01] were the factors influencing readmission of diabetes patients, and the differences were statistically significant. Conclusions:Female, blood glucose, hospital stay, disability, retirement, unemployment, insulin use, low blood sodium, hematocrit, discharge without medical advice, emergency, fluid and electrolyte disorders, heart failure, complications, renal failure, anemia are the factors that affect the readmission of diabetes patients.

ObjectiveTo investigate the feasibility of apical sodium-dependent bile salt transporter (ASBT) and asialoglycoprotein receptor (ASGPR) in the design of oral liver-targeting preparations for the treatment of hepatic alveolar echinococcosis (HAE) by measuring the expression of ASBT and ASGPR. MethodsA total of 18 male Sprague-Dawley rats were selected, among which 10 were used to establish a model of HAE (HAE group) and 8 were used as controls (normal group). Immunofluorescence assay, Western blotting, and quantitative real-time PCR were used to measure the expression distribution, protein expression level, and mRNA expression level of ASBT in the ileal tissue of HAE model rats and normal rats; the same methods were used to measure the expression level of ASGPR in the non-diseased liver tissue and the marginal zone of liver tissue lesion of HAE model rats and the liver tissue of normal rats. The t-test was used for comparison of normally distributed continuous data between two groups; a one-way analysis of variance was used for comparison between three groups, and the least significant difference t-test was used for comparison between two groups. ResultsThe results of immunofluorescence assay, Western blotting, and quantitative real-time PCR showed that compared with the normal group, the HAE group had significantly upregulated expression of ASBT in the ileal tissue (t=5309, 4.110, and 28.060, all P＜0.05) and a significantly higher expression level of ASGPR (the closer to the lesion, the higher the expression) (F=110666, 128.201, and 143.879, all P＜0.001). ConclusionASBT and ASGPR can be used as potential mediated receptors for oral liver-targeting preparations for HAE, which provides a theoretical basis for the design of oral liver-targeting preparations for the treatment of HAE.

Objective:To understand the content of fluorine in tea leachate in Shijiazhuang City.Methods:From 2017 to 2019, different varieties of tea (including 7 varieties of green tea, scented tea, dark tea, oolong tea, black tea, white tea, and yellow tea) with high sales volume were purchased at the Shijiazhuang Tea Market. The fluoride content in tea was determined by fluoride ion selective electrode method through different soaking time (5, 10, 15, 20 min) and soaking times (1, 2, 3 times).Results:A total of 82 tea samples were detected, including 25 kinds of green tea (30.49%), 5 kinds of scented tea (6.10%), 11 kinds of dark tea (13.41%), 17 kinds of oolong tea (20.73%), 15 kinds of black tea (18.29%), 7 kinds of white tea (8.54%) and 2 kinds of yellow tea (2.44%). After soaking for 20 min, the fluorine contents in tea leachate of green tea, oolong tea, black tea, and dark tea (40.63, 65.43, 26.39, 58.90 mg/kg) were significantly higher than those in 5 min(23.09, 32.24, 15.77, 32.00 mg/kg, P < 0.05). Taking the fluorine content of the tea leachate soaking for 20 min as the total fluorine content, oolong tea was the highest, with a median of 65.43 mg/kg; black tea was the lowest, with a median of 26.39 mg/kg; The fluorine contents of oolong tea, dark tea and scented tea were significantly higher than that of black tea( P < 0.05). With the increase of soaking times of green tea, oolong tea, black tea, dark tea and white tea, the fluorine contents in tea leachate decreased significantly ( P < 0.05). Conclusions:In this study, the content of fluorine in the oolong tea is the highest, and the lowest is black tea. It is suggested that local residents in Shijiazhuang should drink black tea.

At present, there are many monitoring devices for vital signs parameters for clinical selection, but urine monitoring has not received enough attention. The traditional manual visual measurement, intermittent measurement of urine pH value, and other methods are still in use, so the authors designed a multifunctional urine bag. The urine bag can set up an alarm system per unit time according to patient′s age and condition as well as set up urine pH value detection area in hard plastic measuring bottle. This device can not only accurately monitor urine volume per unit time, but also hopefully realize the alarm automation of abnormal urine volume per unit time, as well as real-time dynamic monitoring of urine pH value. It is helpful for medical staff to make accurate judgment on patients′ condition changes, to guide the formulation and modification of clinical treatment plans, and to reduce the workload of clinical nursing staff to a certain extent.

Objective :To explore influence of CYP2C19 gene polymorphism on clopidogrel resistance after percutane‐ous coronary intervention (PCI) in patients with coronary heart disease (CHD).Methods :A total of 100 CHD pa‐tients ,who were treated and received PCI in our hospital ,were selected .There were 24 cases with clopidogrel re‐sistance (CR ,CR group) and 76 cases without CR (NCR ,NCR group).According to CYP2C19 genotype ,patients were divided into rapid metabolism CYP2C19*1／*1 (n＝49) ,medium metabolism CYP2C19*1／*2 (n＝28) and*1／*3 (n＝11) ,and slow metabolism CYP2C19*2／*2 (n＝9) and *2／*3 (n＝3).Relationship among differ‐ent genotypes ,CR ,maximum platelet aggregation rate (MPA) and incidence of major adverse cardiovascular events (MACE) were analyzed .Results :With rapid metabolism CYP2C19*1／*1 as the base ,there was significant rise in CR risk in medium metabolism (CYP2C19*1／*2 and *1／*3 ,OR＝4. 16 ,5. 03 , P＜0.05 both) and slow metab‐olism (CYP2C19*2／*2 and *2／*3 ,OR＝7.04 ,17. 6 , P＜0.01 both ) ,medium metabolism increased by 4. 16 and 5. 03 times respectively ,while slow metabolism increased by 7. 04 and 17. 60 times respectively .Compared with rapid metabolism genotype ,there were significant rise in MPA and incidence rate of MACE in medium and slow me‐tabolism genotypes , P＜0.05 or ＜0. 01 ;incidence rate of MACE in CR group was significantly higher than that of NCR group (20. 8% vs.5. 3%, P＝0.02).Conclusion : CYP2C19 gene polymorphism possesses certain influence on CR after PCI in CHD patients .Those with medium or slow metabolism genotypes are more likely to suffer from CR ,higher MPA and incidence rate of MACE .