Objective To investigate the association between extensive perivascular space(EPVS)burden in different locations and ischemic stroke(IS),its subtypes,and transient ischemic attack(TIA)through Mendelian randomization(MR)analysis.Methods The summary data from large-scale Genome-wide Association Studies(GWAS)and various MR methods were employed.We applied multivariable MR to mitigate potential confounding factors and conduct sensitivity analyses to enhance result robustness.Subsequently,meta-analysis was utilized to integrate causal relationships between EPVS burden in different locations and IS from various sources.Additionally,reverse MR was employed to observe the impact of various IS types on EPVS burden.Finally,linkage disequilibrium score regression was conducted to assess genetic correlations between exposures and outcomes.Results EPVS burden in both the white matter(OR=1.12,95％CI:1.01-1.25;P=0.04)and basal ganglia(OR=1.57,95％CI:1.30-1.89;P＜0.01)are significant risk factors for IS.EPVS burden in the basal ganglia is also a risk for IS(small-vessel)(OR=4.56,95％CI:2.57-8.27;P=5.95× 10-7).After IS and TIA there seems to be a potential increase in extensive basal ganglia perivascular space burden.Conclusions Extensive white matter perivascular space burden and extensive basal ganglia perivascular space burden may serve as important indicators to predict IS.

Objective To investigate the association between extensive perivascular space(EPVS)burden in different locations and ischemic stroke(IS),its subtypes,and transient ischemic attack(TIA)through Mendelian randomization(MR)analysis.Methods The summary data from large-scale Genome-wide Association Studies(GWAS)and various MR methods were employed.We applied multivariable MR to mitigate potential confounding factors and conduct sensitivity analyses to enhance result robustness.Subsequently,meta-analysis was utilized to integrate causal relationships between EPVS burden in different locations and IS from various sources.Additionally,reverse MR was employed to observe the impact of various IS types on EPVS burden.Finally,linkage disequilibrium score regression was conducted to assess genetic correlations between exposures and outcomes.Results EPVS burden in both the white matter(OR=1.12,95％CI:1.01-1.25;P=0.04)and basal ganglia(OR=1.57,95％CI:1.30-1.89;P＜0.01)are significant risk factors for IS.EPVS burden in the basal ganglia is also a risk for IS(small-vessel)(OR=4.56,95％CI:2.57-8.27;P=5.95× 10-7).After IS and TIA there seems to be a potential increase in extensive basal ganglia perivascular space burden.Conclusions Extensive white matter perivascular space burden and extensive basal ganglia perivascular space burden may serve as important indicators to predict IS.

This article introduced the background,drafting process,and main content of the Interim Measures for the Management of Surplus Drugs in Medical Institutions of Hubei Province(referred as the Measures).It focused on explaining the definition of surplus drugs and analyzing the requirements for drug dismantling,surplus drug billing,recovery and use procedures,special fund management,and duties and responsibilities of management departments.This paper aimed to guide readers to learn the Measures,understand the Measures and implement the Measures.It would help to improve the efficiency of medical resources,ensure medication safety,reduce patients'medication burden,and promote the rational use of medical insurance funds.

Objective:To observe the changes of emotional state and quality of life in patients with oral cancer surgery after empowerment education combined with motivational psychological intervention.Methods:A total of 80 patients with oral cancer who underwent surgical treatment in the First Affiliated Hospital of Zhengzhou University were enrolled from January to December 2023. According to order of admission and comparability principle of inter-group basic data, the subjects were divided into control group (from January to May 2023, n=40) and observation group (from June to December 2023, n=40). On basis of standardized disease management, control group was given routine health education and psychological intervention, while observation group was given empowerment education combined with motivational psychological intervention. All patients were intervened till 3 months after discharge. Before intervention (admission) and after intervention (3 months after discharge), disease uncertainty, emotional state, coping styles, self-efficacy, compliance and quality of life were evaluated by Mishel uncertainty in illness scale-adult(MUIS-A), self-rating depression scale (SDS), self-rating anxiety scale (SAS), trait coping style questionnaire (TCSQ), general self-efficacy scale (GSES) and University of Washington quality of life questionnaire-4 (UW-QoLv4), respectively. The differences between the two groups (intra-group) were analyzed by SPSS 21.0 statistical software, including independent sample t-test, paired t-test and Chi-square test. Results:(1)The scores of MUIS-A, SDS, SAS, TCSQ, GSES and UW-QoLv4 were not significantly different between the two groups before intervention(all P>0.05).(2)After intervention, scores of MUIS-A, SDS, SAS and TCSQ-negative coping in observation group were 91.56±7.49, 49.83±7.63, 50.54±6.51 and 30.21±3.37, lower than those in control group (100.18±8.52, 56.95±8.11, 58.14±7.36, 32.19±2.36, t=4.806, 4.044, 4.892, 3.044, all P<0.05). The scores of TCSQ-positive coping and GSES in observation group (34.25±2.13 and 29.25±2.83) were higher than those in control group (31.14±2.06, 25.70±3.14, t=6.638, 5.311, both P<0.001). (3)The compliance of dietary management, rehabilitation training and postoperative reexamination in observation group were better than those in control group ( χ2=4.114, 6.646, 5.165, all P<0.05). (4)The incidence of flap necrosis in observation group was lower than that in control group (5.00%(2/40) vs 22.50%(9/40), χ2=5.165, P<0.05). (5)After intervention, UW-QoLv4 score in observation group was higher than that in control group (863.28±24.87 vs 751.26±38.94, t=15.334, P<0.05). Conclusion:Empowerment education combined with motivational psychological intervention can effectively improve the emotional state and quality of life of patients with oral cancer surgery.

Objective:To observe the changes of emotional state and quality of life in patients with oral cancer surgery after empowerment education combined with motivational psychological intervention.Methods:A total of 80 patients with oral cancer who underwent surgical treatment in the First Affiliated Hospital of Zhengzhou University were enrolled from January to December 2023. According to order of admission and comparability principle of inter-group basic data, the subjects were divided into control group (from January to May 2023, n=40) and observation group (from June to December 2023, n=40). On basis of standardized disease management, control group was given routine health education and psychological intervention, while observation group was given empowerment education combined with motivational psychological intervention. All patients were intervened till 3 months after discharge. Before intervention (admission) and after intervention (3 months after discharge), disease uncertainty, emotional state, coping styles, self-efficacy, compliance and quality of life were evaluated by Mishel uncertainty in illness scale-adult(MUIS-A), self-rating depression scale (SDS), self-rating anxiety scale (SAS), trait coping style questionnaire (TCSQ), general self-efficacy scale (GSES) and University of Washington quality of life questionnaire-4 (UW-QoLv4), respectively. The differences between the two groups (intra-group) were analyzed by SPSS 21.0 statistical software, including independent sample t-test, paired t-test and Chi-square test. Results:(1)The scores of MUIS-A, SDS, SAS, TCSQ, GSES and UW-QoLv4 were not significantly different between the two groups before intervention(all P>0.05).(2)After intervention, scores of MUIS-A, SDS, SAS and TCSQ-negative coping in observation group were 91.56±7.49, 49.83±7.63, 50.54±6.51 and 30.21±3.37, lower than those in control group (100.18±8.52, 56.95±8.11, 58.14±7.36, 32.19±2.36, t=4.806, 4.044, 4.892, 3.044, all P<0.05). The scores of TCSQ-positive coping and GSES in observation group (34.25±2.13 and 29.25±2.83) were higher than those in control group (31.14±2.06, 25.70±3.14, t=6.638, 5.311, both P<0.001). (3)The compliance of dietary management, rehabilitation training and postoperative reexamination in observation group were better than those in control group ( χ2=4.114, 6.646, 5.165, all P<0.05). (4)The incidence of flap necrosis in observation group was lower than that in control group (5.00%(2/40) vs 22.50%(9/40), χ2=5.165, P<0.05). (5)After intervention, UW-QoLv4 score in observation group was higher than that in control group (863.28±24.87 vs 751.26±38.94, t=15.334, P<0.05). Conclusion:Empowerment education combined with motivational psychological intervention can effectively improve the emotional state and quality of life of patients with oral cancer surgery.

Objective To explore effect of 5 -aminolevulinic acid -photodynamic therapy combined with thymopentin -5 on IL -17,IL -23 expression of recurrent condylomata acuminata patients.Methods 140 patients with recurrent condylomata acuminata were randomly divided into 3 groups.53 cases in observation group were treated by 5 -aminolevulinic acid -photodynamic therapy combined with thymopentin -5,42 cases in control group 1 were treated by 5 -aminolevulinic acid -photodynamic therapy,and 45 cases in control group 2 were treated by thymopen-tin -5.24 healthy subjects were served as normal controls.IL -17,IL -23 levels were determined by enzyme linked immunosorbent assay before and after the clinical therapy.Results IL -17,IL -23 levels in the patients with recur-rent condylomata acuminata were significantly lower than those in healthy subjects(t =28.10,P <0.01;t =11.10, P <0.01).There were significant differences in IL -17,IL -23 between recurrent condylomata acuminata patients and healthy persons before treatment.There was significant difference after treatment(t =61.17,P <0.01;t =28.02, P <0.01).Conclusion 5 -aminolevulinic acid -photodynamic therapy combined with thymopentin -5 in the treat-ment of recurrent condylomata acuminata inhibited IL -17,IL -23 expression,so as to achieve therapeutic effect.

Objective To investigate the effect of phenylephrine on the morphology and the expression level of Nampt mRNA in the irradiated osteoblasts of the rats,and to clarify the possible protective effects of phenylephrine on this injury.Methods The osteoblasts cultured by conventional tissue were randomly divided into control group, phenylephrine group,simple irradiation group,and phenylephrine+irradiation group.The cells in phenylephrine group were treated with 100 mmol· L-1 phenylephrine 30 min before irradiation.The cells in simple irradiation group were irradiated with 8 Gy X-ray.The osteoblasts were observed by microscope and were collected at the time points of 8,16,32 and 64 h after irradiation.The Nampt mRNA expression level in osteoblasts was examined by RT-PCR.Results There was no obviously morphological and number changes of osteoblasts in phenylephrine group compared with control group.The osteoblasts in simple irradiation group were atrophied,especially at 8 h;the number of osteoblasts in simple irradiation group was 0.82,0.37,0.24 and 0.21 times as control group respectively at 8,16,32 and 64 h. Atrophy of osteoblasts was alleviated in phenylephrine+irradiation group;at 8, 16,32 and 64 h,the number of osteoblasts was 0.91,0.83,0.72 and 0.75 times as control group,and was 1.09,2.24,3.00 and 3.60 times as simple irradiation group. The expression level of Nampt mRNA in the osteoblasts in simple irradiation group was reduced compared with control group (P < 0.01) at 8, 16, 32 and 64 h.There were significant differernces of the expression levels of Nampt mRNA in the osteoblasts between phenylephrine+irradiation group and control group (P < 0.01),and there were significant differernces between phenylephrine + irradiation group and simple irradiation group (P < 0.01 ) at the different time points. Conclusion Phenylephrine could reduce the atrophy of osteoblasts in the rats induced by irradiation and up-regulate the expression level of Nampt mRNA.Its protective effect on irradiated injury may be related to the up-regulation of the expression level of Nampt mRNA.

After thelibrary cloudwas established using thecloud computing-related,virtualization-relat-ed and special communication channel-related techniques , the abundant resources in library were sent to the health institutions, officers and men in the grass-rooted military units through the special virtual network, thus the information resources-sharing was realized in hospitals of station troops and grass-rooted military units.

Objective To investigate the relationship between serum uric acid (UA) level and abdominal obesity or metabolic syndrome (MS).Methods A total of 875 subjects,with 350 males and 525 females,aged 40-65 years old,were enrolled in this study.The clinical and biochemical data were collected and MRI was used to assess the visceral and subcutaneous adipose tissues.The relationships between UA level and abdominal obesity or MS were analyzed,and the cut-off values of UA for abdominal obesity and MS were determined.Results Raised risks of abdominal obesity (OR =4.35,95％ CI 1.91-9.90 in males; OR =5.44,95％ CI 2.41-12.31 in females) and MS (OR =4.47,95 ％ CI 2.08-9.62 in males; OR =11.62,95％ CI 3.43-39.37 in females) were observed with the increase of UA level.The multiple logistic regression analysis showed that UA was an independent risk factor for hypertriglyceridemia (OR =2.23,95％ CI 1.02-4.87 in males ; OR =3.04,95％ CI 1.49-6.23 in females) in all subjects and for abdominal obesity(OR =3.23,95％ CI 1.32-7.91) and hypertension (OR =2.35,95％ CI 1.37-4.05)in the females.Among the females,the regression line analyzed by simple correlation indicated that the UA level of 244.0 μmol/L was corresponded to the visceral adipose tissue area of 80 cm2.The optimal cut-off point of UA for the diagnosis of MS was 258.8 μmol/L determined by the receiver operating characteristic curve.Conclusions The level of UA is closely correlated with abdominal obesity and MS in the middleaged Chinese.The elevated UA level is an independent risk factor for abdominal obesity and MS in the female.

OBJECTIVETo investigate whether the angiotensin II type I receptor gene (AGTR1) A1166C polymorphism is associated with a high risk of diabetic nephropathy.

METHODSThe allele frequency and the genotype distribution of the AGTR1 A1166C polymorphism were studied in normal controls (157 cases), simple diabetes (141 cases, duration of diabetes >10 years), and diabetic nephropathy (152 cases) by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

RESULTSPatients with diabetic nephropathy had a higher frequency of C allele of the AGTR1 A1166C polymorphism than that of normal controls and simple diabetes (P<0.05); but there was no significant difference in frequency of C allele between the normal controls and patients with simple diabetes.

CONCLUSIONThe diabetic patients with AGTR1 C allele may be more susceptible to diabetic nephropathy than diabetic patients with A allele.

Aged ; Diabetic Nephropathies ; genetics ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Humans ; Male ; Middle Aged ; Polymorphism, Genetic ; Receptor, Angiotensin, Type 1 ; genetics