Objective:To investigate the effects of warming needle combined with pelvic floor muscle massage on clinical symptoms,quality of life(QOL),and sex hormone levels in patients with benign prostatic hyperplasia(BPH).Methods:A total of 140 patients diagnosed with BPH were randomly divided into a control group,a warming needle group,a massage group,and a combined treatment group,with 35 patients in each group.The control group was given oral finasteride tablets for treatment.In addition to oral finasteride tablets,the warming needle group was given warming needle treatment,the massage group was given pelvic floor muscle massage,and the combined treatment group was given both warming needle and pelvic floor muscle massage.After two treatment courses,the total effective rate was compared among the four groups,and changes in such clinical indicators as international prostate symptom score(I-PSS),QOL score,total prostate volume(TPV),testosterone(T),estradiol(E2),luteinizing hormone(LH),and prostate-specific antigen(PSA)were observed.Results:The differences in the total effective rate among the four groups indicated statistical significance(P<0.05),and the combined treatment group showed a significantly higher effective rate than the other three groups(P<0.05).After treatment,the I-PSS,QOL score,TPV,and serum levels of T,E2,LH,and PSA in all four groups improved compared to those before treatment(P<0.05),and the extent of improvement in the combined treatment group was significantly greater than that in the other three groups(P<0.05).Conclusion:Oral finasteride tablets,warming needle,and pelvic floor muscle massage can all improve the I-PSS,QOL score,TPV,and serum levels of T,E2,LH,and PSA in patients with BPH,and the combination of the three therapies demonstrates the most significant effect.

Objective:To investigate the effects of warming needle combined with pelvic floor muscle massage on clinical symptoms,quality of life(QOL),and sex hormone levels in patients with benign prostatic hyperplasia(BPH).Methods:A total of 140 patients diagnosed with BPH were randomly divided into a control group,a warming needle group,a massage group,and a combined treatment group,with 35 patients in each group.The control group was given oral finasteride tablets for treatment.In addition to oral finasteride tablets,the warming needle group was given warming needle treatment,the massage group was given pelvic floor muscle massage,and the combined treatment group was given both warming needle and pelvic floor muscle massage.After two treatment courses,the total effective rate was compared among the four groups,and changes in such clinical indicators as international prostate symptom score(I-PSS),QOL score,total prostate volume(TPV),testosterone(T),estradiol(E2),luteinizing hormone(LH),and prostate-specific antigen(PSA)were observed.Results:The differences in the total effective rate among the four groups indicated statistical significance(P<0.05),and the combined treatment group showed a significantly higher effective rate than the other three groups(P<0.05).After treatment,the I-PSS,QOL score,TPV,and serum levels of T,E2,LH,and PSA in all four groups improved compared to those before treatment(P<0.05),and the extent of improvement in the combined treatment group was significantly greater than that in the other three groups(P<0.05).Conclusion:Oral finasteride tablets,warming needle,and pelvic floor muscle massage can all improve the I-PSS,QOL score,TPV,and serum levels of T,E2,LH,and PSA in patients with BPH,and the combination of the three therapies demonstrates the most significant effect.

Objective:To investigate the clinicopathological features of fumarate hydratase (FH) deficiency uterine leiomyoma.Methods:The data of 38 patients with FH deficiency uterine leiomyoma were screened and analyzed. The expressions of FH, S-(2-succino)-cysteine (2SC), desmin, p16, p53, CD 10 and cell proliferation associated nuclear antigen (Ki-67) proteins were detected by immunohistochemistry, and their clinicopathological features were analyzed retrospectively. Results:(1) Clinical features: the median age of the patients was (42.5±7.4) years old. Twenty-one cases (55%) of them were myomas found in physical examination, and the median maximum diameter of the tumor was 6.0 cm (range: 5.0-7.5 cm); myomectomy was performed in 23 cases (61%), total hysterectomy with or without bilateral appendages in 15 cases (39%); laparoscopic surgery in 27 cases (71%), open surgery in 11 cases (29%); none of the patients had renal cell carcinoma. (2) Histological features: atypical nuclear cells were distributed locally or diffusely, eosinophilic nucleoli and intranuclear inclusion bodies could be seen, glass like globules could be seen in the cytoplasm, nuclear division was 0-4/10 high power field (HPF), and antler like blood vessels and pulmonary edema-like changes could be seen in the stroma. Among 38 patients with FH deficiency uterine leiomyoma, FH was negative in 37 cases (97%), and positive in 1 case (3%); 2SC, desmin, p16, p53, CD 10 and Ki-67 showed focal positive expression in 38 cases (100%), including 35 cases (92%) with Ki-67 index<10% and 3 cases (8%) with Ki-67 index ≥10%. (3) Follow-up: 4 cases (11%) recurred, and there was no death. There were significant differences in age, family history, distribution of atypical nuclei and mitosis number between recurrent group and non-recurrent group (all P<0.05). Conclusions:FH deficiency uterine leiomyoma is a rare tumor, which needs pathological examination,immunohistochemical examination and clinical history. Patients younger than 43 years old, with family history, histologically atypical diffuse nuclear distribution and mitotic number ≥3/10 HPF should be alert to the risk of recurrence.

Objective:To investigate the changes and clinical significance of white blood cell (WBC) count and polymorphonuclear cells (PMN) proportion of synovial fluid after primary total knee arthroplasty (TKA).Methods:A total of 59 patients (92 knees) who underwent TKA from April 2021 to July 2021 were included, including 13 males (20 knees) and 46 females (72 knees). The patients were with average age 65.17±7.49 years old (range 48-79) and with body mass index (BMI) 27.64±3.74 kg/m 2 (range 17-36 kg/m 2). There were 26 cases involved lateral knee and 33 cases bilateral knees. The left knee was involved in 46 knees, while other 46 were involved in right side. There were 54 patients diagnosed as osteoarthritis (84 knees) and 5 as rheumatoid arthritis (8 knees). None of the patients received antibiotics after hospitalization. Antibiotics were used prophylactically 30 min before surgery and after surgery. The synovial fluid WBC count and PMN proportion were detected during operation (It reflects the normal preoperative level) and on the 2nd, 5th and 35th day after operation. The changes of these indexes were analyzed. The unmixed synovial fluid was collected after the incision of the joint capsule during the operation. The outer upper edge of the patella was taken as the puncture point to extract the synovial fluid on the 2nd, 5th and 35th days after the operation. The final follow-up end point was a diagnosis of acute periprothetic joint infection (PJI) or 90 days of follow-up for patients without PJI. Results:After operation, the synovial fluid WBC count and PMN proportion showed a trend of increasing and then decreasing. The synovial fluid WBC count were 0.222(0.100, 0.567)×10 9/L, 20.011(14.573, 29.129)×10 9/L, 6.080(3.676, 8.797)×10 9/L, 0.533(0.394, 0.749)×10 9/L before surgery and at 2nd, 5th and 35th day after surgery respectively. The difference before and after operation was statistically significant (χ 2=247.343, P<0.001). The synovial fluid PMN proportion were 19.9%(15.0%, 30.0%), 96.0%(95.0%, 97.0%), 91.0%(89.0%, 93.0%) and 20.5%(15.6%, 26.9%) respectively and with significant difference (χ 2=242.521, P<0.001). Pairwise comparison of synovial fluid WBC count and PMN proportion before and on day 35 indicated no statistical significance ( P>0.05). However, the differences compared with that at other time points were statistically significant ( P<0.05). Conclusion:The synovial fluid WBC count and PMN proportion increased rapidly in the short term after TKA and then decreased to the preoperative level at varied rates. Referring to the Musculoskeletal Infection Society (MSIS) recommended threshold for diagnosing infection during the acute stress period (within 5 d postoperatively) can result in false positives. Recovery to preoperative levels at 35th days postoperatively can be used as a basis for ruling out early infection. Thus, early joint fluid PMN proportion may have more diagnostic value than WBC counts.

Hepatitis B virus (HBV) infection is closely associated with the adverse events such as liver cirrhosis, liver cancer, and liver failure and remains a serious threat to human health. Pegylated interferon is an indispensable drug for the treatment of chronic hepatitis B (CHB), and interferon-stimulated genes are associated with a variety of viruses, but few studies have mentioned their association with hepatitis B and their predictive effect after the treatment of hepatitis B with interferon. This article introduces the predictive factors for interferon treatment of CHB and summarizes the association of interferon-stimulated genes with hepatitis B and their predictive effect, so as to provide a reference for clinical work and basic research.

Objective: To investigate epidemiological features of genital Chlamydia trachomatis(Ct) infection among youth aged 15-24 years in Beijing, so as to provide scientific basis for developing control strategies. Methods: Data regarding genital Ct infection among youth aged 15-24 years were collected from the disease surveillance information reporting system in Beijing between 2010 and 2019 and were analyzed with the descriptive epidemiological method. Results: During 2010-2019, a total of 1 871 cases of Ct infection was reported, 1 287(68.79%) of the cases were female. The reported incidence rate of genital Ct infection increased from 3.16 per 100 000 in 2010 to 25.15 per 100 000 in 2019, with an average annual increasing rate of 25.92%( χ 2=836.86, P < 0.01 ). Increasing trends in the reported incidence were observed among males and females, females being higher than males in the year 2010 and 2012. The reported incidence rates increased annually in all age groups between 2010 and 2019, and the average annual rate of increase was highest in the group aged 15-19 years. Conclusion The reported rate of genital Ct infection showed an increasing trend among youth aged 15-24 years in Beijing, and increased rapidly among youth aged 15-24 years. More attention should be paid on sexual and reproductive health education among adolescents. Epidemic of genital Ct infection should be concerned, and its surveillance need to be improved.

Objective To investigate the correlation between late-onset sporadic parkinson's disease(PD) and single nucleotide polymorphism (SNP) of autophagy-related genes 7 (Atg7) rs2606757 (6+1196A/T)in Han Chinese population.Methods Totally 124 patients with late-onset sporadic PD(the PD group)and 105 age-and sex-matched healthy individuals(the control group)were enrolled in this study.The SNP of Atg7 rs2606757 was detected by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).After gene sequencing for the detection of allele and genotype frequencies distribution and testing Hardy-Weinberg equilibrium,the differences in Atg7 rs2606757 genotype and allele frequency were compared between PD group and control group and between males and females.Results The frequency of AA genotype was statistically significantly lower in the PD group[34.7％(43/124)]than in the control group[53.3 ％ (56/105)](x2 =8.063,P=0.005,OR =0.465,95％ CI:0.273-0.791).In men's PD group versus men's control group,AA genotype of the Atg7 rs2606757 showed a lower frequency for late-onset sporadic PD[33.3 ％ (23/69)vs.53.2 ％ (33/62),x2 =5.280,P =0.022,OR =0.439,95 ％ CI:0.217-0.891].Logistic regression analysis indicated that the AA genotype frequency distribution of Atg7 rs2606757 showed a significant difference between PD and control groups (OR =2.210,95％ CI:1.289-3.789,P =0.004).Conclusions The higher frequence of AA genotype at Atg7 rs2606757 only in males might be associated with the decreased risk of late-onset sporadic PD.

High-voltage-activated (HVA) Ca channels are widely expressed in the nervous system. They play an important role in pain conduction by participating in various physiological processes such as synaptic transmission, changes in synaptic plasticity, and neuronal excitability. Available evidence suggests that the HVA channel is an important therapeutic target for pain management. In this review, we summarize the changes in different subtypes of HVA channel during pain and present the currently available evidence from the clinical application of HVA channel blockers. We also review novel drugs in various phases of development. Moreover, we discuss the future prospects of HVA channel blockers in order to promote "bench-to-bedside" translation.

Objective To investigate the association between rs14016 (19 +31C/T) polymorphisms of autophagy-related genes 7 (Atg7) and Parkinson's disease (PD) in Han population in China.Methods Totally 123 patients with Parkinson's disease (PD) (case group) and 101 synchronized health controls (control group) were selected from Chinese Han population between January 2013 and July 2016.A single nucleotide polymorphism (SNP) of rs14016 of Atg7 gene was detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis.After gene sequencing for genotyping and detection of alleles,genotype and allele frequency distributions were analyzed in the two groups.Results The frequency distributions of TT genotype and T alleles were 17.9％ (22/123) and 41.1％ (101/246) in the case group,and 5.9％ (6/101) and 31.2 ％ (63/202) in the control group,respectively.The difference in genotype frequencies between the two groups was statistically significant (x2 =7.236,P =0.007,OR =3.01,95 ％ CI:1.27-7.14).The frequencies of T and C alleles were 41.1％ (101/246) and 58.9％ (145/246) respectively in the case group,and 31.2％ (63/202) and 68.8％ (139/202) in the control group (x2 =4.655,P=0.031,OR=1.32,95 ％CI:1.02-1.70),with the statistically significant difference in the allele.The TT genotype of rs14016 showed statistical significance between the two groups by logistic regression analysis (OR=3.40,95％CI:1.32-8.80,P=0.012).Conclusions The T allele and TT genotype at the rs14016 of Atg7 gene might be associated with PD,and might increase the risk for suffering from PD,which is worthy of further fully researches.

Objective To understand the academic atmosphere current situation of graduate students and put forward relevant countermeasures to protect the academic integrity of graduate students.Methods The graduate student'scientific ethics and academic atmosphere construction situation of graduate students in 10 colleges and universities in Chongqing were investigated by questionnaire survey and analyzed.Results Through 1555 questionnaires,it was found that the average investment of time on scientific research in gruduate students was insufficient,the motivation of learning was complicated,and the situation of preventing academic misconduct was serious.Conclusion This study suggests that colleges and universities should establish an academic integrity security system for graduate students,including establishment of academic integrity system and specializing academic integrity supervision institutions,and strengthing academic integrity education.