To investigate and analyze the distribution characteristics and genetic variation of mitochondrial encephalomyopathy (ME) in children in Hainan province.

Objective:To explore the short-term clinical effects of digitally guided anterior teeth porcelain veneer restoration.Methods:17 patients with 80 teeth treated by digitally guided anterior teeth porcelain veneer restoration were included and divided into minimally invasive group(A,9 cases with 40 teeth)and the invasive group(B,8 cases with 40 teeth).The accuracy of tooth preparation guides was evaluated by Bland-Altman analysis,the clinical effects at 1 week and 3,6 and 12 months and the plaque in-dex at 12 months after operation were compared between the two groups.Results:The mean error of tooth preparation by the guide plate is(0.089±0.029)mm,and the mean differences in cervical and middle were smaller than that in incisal area.There were sta-tistically significant differences between the two groups in the follow-up clinical effect scores of color matching,gingival health and postoperative sensitivity(P＜0.05).The plaque index at 12 months after surgery was 0.67±0.41 in the minimally invasive group and 0.88±0.47 in the invasive group(P=0.026).Conclusion:The digitally guided porcelain veneer restoration realizes the visualiza-tion of tooth preparation and has good short-term clinical effects.

Objective:To observe the therapeutic efficacy of point catgut-embedding therapy combined with cupping therapy in treating simple obesity due to stomach heat and dampness obstruction,as well as its effects on Chinese medicine symptoms,obesity indicators,and blood lipid parameters.Methods:A total of 135 cases with simple obesity due to stomach heat and dampness obstruction were allocated into an embedding group,a cupping group,and a combined group using the random number table method,with 45 cases in each group.The embedding group received point catgut-embedding therapy alone,the cupping group was treated with cupping therapy alone,while the combined group underwent both embedding therapy and cupping therapy.The same points were used in the three groups.The treatment was performed once daily,and consecutive 28-day treatment was taken as 1 treatment course.The therapeutic efficacy was evaluated,and the changes in the traditional Chinese medicine(TCM)symptom score,obesity indicators[body mass(BM),body mass index(BMI),body fat percentage(BFP),waist circumference,and waist-to-hip ratio],and blood lipid parameters[triglyceride(TG)and total cholesterol(TC)]were observed after 3 consecutive courses.Results:After 3-course treatment,the total effective rate in the combined group was 93.3%,higher than 82.2%in the embedding group and 73.3%in the cupping group(P<0.05),and the total effective rate of the embedding group was higher than that of the cupping group(P<0.05).Compared to those before treatment,the TCM symptom score,obesity indicators,and blood lipid parameters reduced significantly in the three groups(P<0.05 or P<0.01),and indicators in the combined group were lower than those in the other two groups(P<0.05).Conclusion:The synergistic application of point catgut-embedding therapy and cupping therapy can improve TCM symptoms,obesity-related indicators,and blood lipid parameters of patients with simple obesity due to stomach heat and dampness obstruction;compared to the use of either point catgut-embedding therapy or cupping therapy alone,the combined application of the two therapies shows a more significant therapeutic effect.

Objective:To observe the therapeutic efficacy of point catgut-embedding therapy combined with cupping therapy in treating simple obesity due to stomach heat and dampness obstruction,as well as its effects on Chinese medicine symptoms,obesity indicators,and blood lipid parameters.Methods:A total of 135 cases with simple obesity due to stomach heat and dampness obstruction were allocated into an embedding group,a cupping group,and a combined group using the random number table method,with 45 cases in each group.The embedding group received point catgut-embedding therapy alone,the cupping group was treated with cupping therapy alone,while the combined group underwent both embedding therapy and cupping therapy.The same points were used in the three groups.The treatment was performed once daily,and consecutive 28-day treatment was taken as 1 treatment course.The therapeutic efficacy was evaluated,and the changes in the traditional Chinese medicine(TCM)symptom score,obesity indicators[body mass(BM),body mass index(BMI),body fat percentage(BFP),waist circumference,and waist-to-hip ratio],and blood lipid parameters[triglyceride(TG)and total cholesterol(TC)]were observed after 3 consecutive courses.Results:After 3-course treatment,the total effective rate in the combined group was 93.3%,higher than 82.2%in the embedding group and 73.3%in the cupping group(P<0.05),and the total effective rate of the embedding group was higher than that of the cupping group(P<0.05).Compared to those before treatment,the TCM symptom score,obesity indicators,and blood lipid parameters reduced significantly in the three groups(P<0.05 or P<0.01),and indicators in the combined group were lower than those in the other two groups(P<0.05).Conclusion:The synergistic application of point catgut-embedding therapy and cupping therapy can improve TCM symptoms,obesity-related indicators,and blood lipid parameters of patients with simple obesity due to stomach heat and dampness obstruction;compared to the use of either point catgut-embedding therapy or cupping therapy alone,the combined application of the two therapies shows a more significant therapeutic effect.

Bacillus is a class of spore-producing Gram-positive bacteria that produce a variety of antimicrobial substances with different structures and functions. The application of the antimicrobial substances produced by Bacillus can effectively inhibit the activity of harmful bacteria and fungi and promote the sustainable development of green agriculture. The antimicrobial substances produced by Bacillus mainly include proteins, lipopeptides, polyketones, and polypeptides. This paper reviews the synthesis gene clusters, synthesis pathways, structures, and mechanisms of various antimicrobial substances produced by Bacillus and discusses the challenges in the industrial application of these antimicrobial substances. Furthermore, this paper clarifies the future research and development focuses and prospects the application prospects, and provides comprehensive theoretical support for the in-depth research and wide application of the antimicrobial substances produced by Bacillus.
Bacillus/genetics* ; Anti-Infective Agents/metabolism* ; Bacterial Proteins/genetics* ; Antimicrobial Peptides/biosynthesis* ; Lipopeptides/biosynthesis*

Turning to critical illness is a common stage of various diseases and injuries before death. Patients usually have complex health conditions, while the treatment process involves a wide range of content, along with high requirements for doctor′s professionalism and multi-specialty teamwork, as well as a great demand for time-sensitive treatments. However, this is not matched with critical care professionals and the current state of medical care in China. Telemedicine, which shortens the distance of medical professionals and the gap of disease diagnosis and treatments in various regions through electronic information, can effectively solve the current problem. Therefore, there is an urgent need to develop a standardized, high-quality visualization telemedicine round system .Therefore, experts have been organized to search domestic and foreign literature on telemedicine round for critically ill patients and to form this consensus based on clinical experiences so as to further improve the level of critical care treatments in regions.

Objective:To explore the short-term clinical effects of digitally guided anterior teeth porcelain veneer restoration.Methods:17 patients with 80 teeth treated by digitally guided anterior teeth porcelain veneer restoration were included and divided into minimally invasive group(A,9 cases with 40 teeth)and the invasive group(B,8 cases with 40 teeth).The accuracy of tooth preparation guides was evaluated by Bland-Altman analysis,the clinical effects at 1 week and 3,6 and 12 months and the plaque in-dex at 12 months after operation were compared between the two groups.Results:The mean error of tooth preparation by the guide plate is(0.089±0.029)mm,and the mean differences in cervical and middle were smaller than that in incisal area.There were sta-tistically significant differences between the two groups in the follow-up clinical effect scores of color matching,gingival health and postoperative sensitivity(P＜0.05).The plaque index at 12 months after surgery was 0.67±0.41 in the minimally invasive group and 0.88±0.47 in the invasive group(P=0.026).Conclusion:The digitally guided porcelain veneer restoration realizes the visualiza-tion of tooth preparation and has good short-term clinical effects.

Turning to critical illness is a common stage of various diseases and injuries before death. Patients usually have complex health conditions, while the treatment process involves a wide range of content, along with high requirements for doctor′s professionalism and multi-specialty teamwork, as well as a great demand for time-sensitive treatments. However, this is not matched with critical care professionals and the current state of medical care in China. Telemedicine, which shortens the distance of medical professionals and the gap of disease diagnosis and treatments in various regions through electronic information, can effectively solve the current problem. Therefore, there is an urgent need to develop a standardized, high-quality visualization telemedicine round system .Therefore, experts have been organized to search domestic and foreign literature on telemedicine round for critically ill patients and to form this consensus based on clinical experiences so as to further improve the level of critical care treatments in regions.

Objective:To analyze immune reconstitution and influencing factors in HIV infected men who have sex with men (MSM) with access to antiviral therapy (ART) in Guangxi Zhuang Autonomous Region (Guangxi) during 2005-2021.Methods:The data were collected from Chinese Disease Prevention and Control Information System. The study subjects were HIV infected MSM with access to the initial ART for ≥24 weeks in Guangxi from 2005 to 2021 and HIV RNA lower than the detection limit within 24 months. The proportion of infected MSM who had immune reconstitution after ART was calculated. Cox proportional hazard regression model was used to analyze the influencing factors of immune reconstitution. Software SPSS 24.0 was used for statistical analysis.Results:A total of 3 200 HIV infected MSM were enrolled, in whom 15.56 % (498/3 200) had no immune reconstitution, 14.78% (473/3 200) had moderate immune reconstitution, and the rate of complete immune reconstitution was 69.66% (2 229/3 200). The M ( Q1, Q3) of ART time for immune reconstitution was 12 (5, 27) months. Multivariate Cox proportional risk regression model analysis results showed that compared with those with initial ART at age ≥30 years, WHO clinical stage Ⅲ/Ⅳ illness, baseline BMI <18.50 kg/m 2 and baseline CD4 +T lymphocyte (CD4) counts <200 cells/μl, HIV infected MSM with initial ART at age <30 years, WHO clinical stageⅠ/Ⅱ illness, baseline BMI≥24.00 kg/m 2 and baseline CD4 counts ≥200 cells/μl were more likely to have complete immune reconstitution. Conclusions:In the HIV infected MSM in Guangxi, failures to achieve moderate and complete immune reconstitution were observed. Surveillance and ART regimen should be improved for key populations, such as those with older age and low baseline CD4 counts.

From the perspective of human development history， epidemic diseases have been accompanied by human life and reproduction， causing serious threats to human health and life safety. Epidemic diseases have a rapid onset and are highly infectious， once they occur， can quickly spread to the people around them， causing many people to be infected in a short time. Pulmonary system epidemic disease is the kind of disease with the highest incidence， prevalence， and infectivity among epidemic diseases， which will also have an impact on the economic and social development and even the historical process. Overview of ancient Chinese literature， the wisdom of ancient doctors has certain reference value for possible related work today or in the future. Starting from sorting out the classical theories recorded in the ancient books of traditional Chinese medicine， this paper dug deeply into them and re⁃understood them， with a view to providing a reference for clinical diagnosis and treatment， and public health services.