Objective To evaluate the effectiveness of thermal tomography in breast cancer (BC) screening. Methods We conducted a general population-based BC screening in three regions of Hubei Province (Xiantao, Hongan, and Yangxin Districts). Participants underwent a questionnaire-based interview for baseline data collection. They then received a physical examination, thermal tomography, and ultrasound from doctors and technicians. We compared the efficacies, including sensitivity, specificity, and false-positive rates, of ultrasound and thermal tomography in BC screening. Results A total of 59 712 eligible women were included in this screening program. The BI-RADS 1, 2, 3, 4, and 5 accordance rates between the two screening methods were 0.9549, 0.8047, 0.9037, 0.3352, and 0, respectively. The overall accordance rate was 0.933 1. The kappa consistency results showed that the Cicchetti-Allison and Fleiss-Cohen kappa values were 0.7970 and 0.8583, respectively. Although the two methods had equal sensitivities, specificity was higher in thermal tomography than in ultrasound. The false-positive rate was lower in thermal tomography than in ultrasound. The area under the receiver operating characteristic (ROC) curve of thermal tomography was significantly larger than that of ultrasound. Conclusion The general consistency between thermal tomography and ultrasound in BC screening was high. Thermal tomography outperformed ultrasound in terms of specificity and diagnostic efficiency. Therefore, thermal tomography has a high application value for general population-based BC screening.

Bilateral tubal pregnancy is a rare ectopic pregnancy that occurs in both fallopian tubes. In order to explore the clinical diagnosis and treatment methods, pathological analysis, and treatment outcomes of the disease, we retrospectively analyzed the clinical data of 9 cases of bilateral tubal pregnancy admitted to the Obstetrics and Gynecology Department of Obstetrics & Gynecology Hospital of Fudan University from January 2003 to December 2023. All cases were confirmed as bilateral tubal pregnancy by preoperative ultrasound, and all with a history of embryo transfer, which pregnancy sites distributed in the ampulla and interstitial regions of the fallopian tubes. The treatment method was bilateral salpingectomy. After treatment, there were no cases of secondary surgery, and one case successfully gave birth. In summary, as a rare and atypical type of ectopic pregnancy, bilateral tubal pregnancy should be detected and diagnosed early. Personalized treatment plans should be developed based on the patient's condition and needs. After treatment, changes in blood β-human chorionic gonadotropin should be monitored strictly, and auxiliary examination methods such as Doppler ultrasound should be used to alert to the occurrence of persistent ectopic pregnancy and even trophoblastic diseases

Bilateral tubal pregnancy is a rare ectopic pregnancy that occurs in both fallopian tubes. In order to explore the clinical diagnosis and treatment methods, pathological analysis, and treatment outcomes of the disease, we retrospectively analyzed the clinical data of 9 cases of bilateral tubal pregnancy admitted to the Obstetrics and Gynecology Department of Obstetrics & Gynecology Hospital of Fudan University from January 2003 to December 2023. All cases were confirmed as bilateral tubal pregnancy by preoperative ultrasound, and all with a history of embryo transfer, which pregnancy sites distributed in the ampulla and interstitial regions of the fallopian tubes. The treatment method was bilateral salpingectomy. After treatment, there were no cases of secondary surgery, and one case successfully gave birth. In summary, as a rare and atypical type of ectopic pregnancy, bilateral tubal pregnancy should be detected and diagnosed early. Personalized treatment plans should be developed based on the patient's condition and needs. After treatment, changes in blood β-human chorionic gonadotropin should be monitored strictly, and auxiliary examination methods such as Doppler ultrasound should be used to alert to the occurrence of persistent ectopic pregnancy and even trophoblastic diseases

Objective To investigate the effect of hydrogen sulfide(H2S)on olfactory function in mice and the recovery of olfactory function after H2S poisoning test.Methods Thirty-six clean grade BALB/c mice were randomly divided into control group,0.05 mg/L H2S exposure group,and 5.00 mg/L H2S exposure group according to the random number table method,with 12 mice in each group.The recovery of olfactory function was observed after H2S exposure cycle.The behavior and foraging time of the mice were recorded.Results Before the exposure test,there was no significant difference in olfaction time among the groups(P>0.05).On the 10th,20th and 30th day of the exposure test,the foraging time of 5.00 mg/L H2S exposure group was gradually prolonged,which was significantly different from that of the control group and 0.05 mg/L H2S exposure group(P<0.05).On the 5th,10th and 15th day after exposure test,the foraging time of 5.00 mg/L H2S exposure group gradually shortened,which was significantly longer than that of the control group and 0.05 mg/L H2S exposure group(P<0.05).Conclusion The short-term toxicity test of 5.00 mg/L H2S can cause olfactory dysfunction in mice,manifested by differences in foraging time.This olfactory dysfunction can be recovered to some extent within 15 days after leaving the exposed environment.

Purpose To explore the feasibility of targeted diagnosis and localization of prostate cancer via background free differential ultrasound molecular imaging based on prostate-specific membrane antigen (PSMA) targeted ultrasound nanobubbles (NB). Materials and Methods Targeted PSMA-NB and non-targeted NB were constructed. The targeting ability of PSMA-NB on human prostate tumor 22RV1 cells (PSMA positive expression) and PC-3 cells (PSMA negative expression) was determined in vitro. Ten nude mouse models of human prostate tumor 22RV1 cells (n=5) and PC-3 cells (n=5) were constructed. PSMA-NB was injected into the rat tail vein,and in-situ blasting was performed. Ultrasound molecular images before and after blasting were collected,using destruction supplement post-processing technology to obtain and compare the differential ultrasound molecular imaging effects between the two groups. Results The particle size of PSMA-NB and NB were (363.7±24.4) nm and (236.0±55.2) nm,with statistical difference (t=3.19,P=0.007),respectively. Cell targeting results showed that PSMA-NB only adhered to the nucleus with positive PSMA-expression. Animal experiments indicated that the differential ultrasonic molecular images of PSMA positive expression group only showed the highly enhanced area of contrast agent at the tumor site,with no background noise. Conclusion Background free differential ultrasound molecular images can be used for precise targeted diagnosis and localization of PSMA positive prostate cancer,which is constructed based on PSMA targeted ultrasound nanobubbles.

Purpose To explore the feasibility of targeted diagnosis and localization of prostate cancer via background free differential ultrasound molecular imaging based on prostate-specific membrane antigen (PSMA) targeted ultrasound nanobubbles (NB). Materials and Methods Targeted PSMA-NB and non-targeted NB were constructed. The targeting ability of PSMA-NB on human prostate tumor 22RV1 cells (PSMA positive expression) and PC-3 cells (PSMA negative expression) was determined in vitro. Ten nude mouse models of human prostate tumor 22RV1 cells (n=5) and PC-3 cells (n=5) were constructed. PSMA-NB was injected into the rat tail vein,and in-situ blasting was performed. Ultrasound molecular images before and after blasting were collected,using destruction supplement post-processing technology to obtain and compare the differential ultrasound molecular imaging effects between the two groups. Results The particle size of PSMA-NB and NB were (363.7±24.4) nm and (236.0±55.2) nm,with statistical difference (t=3.19,P=0.007),respectively. Cell targeting results showed that PSMA-NB only adhered to the nucleus with positive PSMA-expression. Animal experiments indicated that the differential ultrasonic molecular images of PSMA positive expression group only showed the highly enhanced area of contrast agent at the tumor site,with no background noise. Conclusion Background free differential ultrasound molecular images can be used for precise targeted diagnosis and localization of PSMA positive prostate cancer,which is constructed based on PSMA targeted ultrasound nanobubbles.

Objective:To study and develop the formulation of a plant material-based deodorant, and to evaluate its deodorization effect.Methods:This study developed a formulation of plant material-based deodorant, detected the main volatile components of plant essential oil, and tested the removal rate of ammonia, hydrogen sulfide, and methyl mercaptan.Results:Within the formulation, 33 active components were detected in the plant essential oil, most of which were oxygen-containing compounds. It was found that 6% of plant essential oil, 1.0% of β-cyclodextrin, and 3.0% of Tween-80 constituted the optimal formulation. The average purification efficiency of the plant material-based deodorant was higher than those of deionized water and chemical deodorant. At 10 minutes after spraying, the efficiency of eliminating hydrogen sulfide and methyl mercaptan of the plant material-based deodorant reached 100%; at 30 minutes after spraying, its efficiency of eliminating ammonia reached 95%.Conclusion:The plant material-based deodorant contains some functional groups of high reactive activity, which can react with odorous substances in various chemical ways to inhibit odors. Its effect of eliminating hydrogen sulfide and methyl mercaptan was better than that of eliminating ammonia.

Objective:To study and develop the formulation of a plant material-based deodorant, and to evaluate its deodorization effect.Methods:This study developed a formulation of plant material-based deodorant, detected the main volatile components of plant essential oil, and tested the removal rate of ammonia, hydrogen sulfide, and methyl mercaptan.Results:Within the formulation, 33 active components were detected in the plant essential oil, most of which were oxygen-containing compounds. It was found that 6% of plant essential oil, 1.0% of β-cyclodextrin, and 3.0% of Tween-80 constituted the optimal formulation. The average purification efficiency of the plant material-based deodorant was higher than those of deionized water and chemical deodorant. At 10 minutes after spraying, the efficiency of eliminating hydrogen sulfide and methyl mercaptan of the plant material-based deodorant reached 100%; at 30 minutes after spraying, its efficiency of eliminating ammonia reached 95%.Conclusion:The plant material-based deodorant contains some functional groups of high reactive activity, which can react with odorous substances in various chemical ways to inhibit odors. Its effect of eliminating hydrogen sulfide and methyl mercaptan was better than that of eliminating ammonia.

Metastasis is the leading cause of human cancer deaths. Unfortunately, no approved drugs are available for anti-metastatic treatment. In our study, high-throughput sequencing-based high-throughput screening (HTS) and a breast cancer lung metastasis (BCLM)-associated gene signature were combined to discover anti-metastatic drugs. After screening of thousands of compounds, we identified Ponatinib as a BCLM inhibitor. Ponatinib significantly inhibited the migration and mammosphere formation of breast cancer cells in vitro and blocked BCLM in multiple mouse models. Mechanistically, Ponatinib represses the expression of BCLM-associated genes mainly through the ERK/c-Jun signaling pathway by inhibiting the transcription of JUN and accelerating the degradation of c-Jun protein. Notably, JUN expression levels were positively correlated with BCLM-associated gene expression and lung metastases in breast cancer patients. Collectively, we established a novel approach for the discovery of anti-metastatic drugs, identified Ponatinib as a new drug to inhibit BCLM and revealed c-Jun as a crucial factor and potential drug target for BCLM. Our study may facilitate the therapeutic treatment of BCLM as well as other metastases.

Objective To understand the comparability of the detection results of four items (ALT ,AST , GGT ,ALP) of liver enzymology in 11 clinical laboratories in Xinjiang Production and Construction Corps (XPCC) and offer reference for improving mutual recognition of the results .Methods Eleven clinical labora-tories of XPCC organized the result comparability tests of 4 items of liver enzymology twice in 2017 ,and the samples with 5 batches were completed in each comparability test .One set of detection system in each labora-tory was used as comparability system according to comparability scheme .The detection results were analyzed through Robust Z Score and the evaluation criterion was :|Z|≤2 "satisfied";2< |Z|<3"warning";|Z|≥3 "not satisfied".Results The detection results of all 10 batch samples in 4 clinical laboratories showed |Z|≤2 in 2 comparability tests .In the first comparability test ,the detection results of 5 batch samples for 4 items were |Z|≤2 in 5 laboratories .In the second comparability test ,the detection results of 5 batch samples for 4 i-tems were |Z|≤2 in 8 laboratories ,but the ALT results of 5 batch samples in 1 laboratory showed positive deviation(Z≥3)and the GGT results of 5 batch samples in the other laboratory showed negative deviation (Z≤ -3) .Conclusion The 11 clinical laboratories in XPCC should continuously improve quality management system and make sure that the mutual recognition of the detection results of 4 items of liver enzymology is effective .