Objective:To explore the changes in serum neuron-specific enolase（NSE）in patients with moderately severe to profound idiopathic sudden sensorineural hearing loss（ISSHL）and the effect of hyperbaric oxygen（HBO）therapy on NSE，and to analyze the relationship between hearing recovery and serum NSE levels.Methods:Using convenient sampling，a total of 90 inpatients with unilateral moderately severe to profound ISSHL were selected as the research subjects，and they were divided into observation group and control group according to the random number table method，with 45 cases in each group. The control group received hormones，Ginkgo biloba injection，and neurotropic drugs，and the observation group was treated with HBO based on the treatment of the control group. Pure tone audiometry was performed before and after treatment to test hearing levels，and serum NSE was determined by ELISA. After 20 days of treatment，the effective rates and the changes of NSE of the two groups were compared，and the relation between NSE level and hearing change were analyzed.Results:The serum NSE levels of ISSHL patients in both groups were significantly higher than those of the control group，and it was correlated with the severity of hearing loss（ P<0.01）. The serum NSE levels of profound ISSHL patients were significantly higher than those of moderately severe and severe ISSHL patients（ P<0.01）. After treatment，the clinical effective rate of the observation group（82.2%）was significantly higher than that of the control group（60.0%）（ P<0.05）；the hearings of the patients in both groups were significantly improved compared with those before treatment；the pure tone average of the observation group was lower than that of the control group（ P<0.05）；and the hearing threshold gain of the observation group was significantly higher than that of the control group（ P<0.01）. After treatment，the NSE levels of the patients in the two groups decreased after treatment；the NSE level of the observation group was significantly lower than that of the control group（ P<0.05）；and the decrease of NSE level in the observation group was significantly greater than that of the control group（ P<0.01）. The decreases of serum NSE in the two groups were positively correlated with the gains of the auditory domain（ r=0.686， P<0.01 in the observation group； r=0.418， P<0.01 in the control group）. Conclusion:The serum NSE level of patients with moderately severe to profound ISSHL is increased，which is related to the severity of hearing loss. Additionally adopting HBO therapy could improve the hearing of patients，increase the effective rate，and reduce the NSE level of ISSHL patients.

Objective:To explore the changes in serum neuron-specific enolase（NSE）in patients with moderately severe to profound idiopathic sudden sensorineural hearing loss（ISSHL）and the effect of hyperbaric oxygen（HBO）therapy on NSE，and to analyze the relationship between hearing recovery and serum NSE levels.Methods:Using convenient sampling，a total of 90 inpatients with unilateral moderately severe to profound ISSHL were selected as the research subjects，and they were divided into observation group and control group according to the random number table method，with 45 cases in each group. The control group received hormones，Ginkgo biloba injection，and neurotropic drugs，and the observation group was treated with HBO based on the treatment of the control group. Pure tone audiometry was performed before and after treatment to test hearing levels，and serum NSE was determined by ELISA. After 20 days of treatment，the effective rates and the changes of NSE of the two groups were compared，and the relation between NSE level and hearing change were analyzed.Results:The serum NSE levels of ISSHL patients in both groups were significantly higher than those of the control group，and it was correlated with the severity of hearing loss（ P<0.01）. The serum NSE levels of profound ISSHL patients were significantly higher than those of moderately severe and severe ISSHL patients（ P<0.01）. After treatment，the clinical effective rate of the observation group（82.2%）was significantly higher than that of the control group（60.0%）（ P<0.05）；the hearings of the patients in both groups were significantly improved compared with those before treatment；the pure tone average of the observation group was lower than that of the control group（ P<0.05）；and the hearing threshold gain of the observation group was significantly higher than that of the control group（ P<0.01）. After treatment，the NSE levels of the patients in the two groups decreased after treatment；the NSE level of the observation group was significantly lower than that of the control group（ P<0.05）；and the decrease of NSE level in the observation group was significantly greater than that of the control group（ P<0.01）. The decreases of serum NSE in the two groups were positively correlated with the gains of the auditory domain（ r=0.686， P<0.01 in the observation group； r=0.418， P<0.01 in the control group）. Conclusion:The serum NSE level of patients with moderately severe to profound ISSHL is increased，which is related to the severity of hearing loss. Additionally adopting HBO therapy could improve the hearing of patients，increase the effective rate，and reduce the NSE level of ISSHL patients.

Objective: This study aimed to evaluate the clinical performance of p16/Ki-67 dual staining for triage high risk HPV (HR-HPV) infected women. Method: Target objects were women who infected HR-HPV and received colposcopy examination between April and December of 2016 at the Second Affiliated Hospital of Zhengzhou University. Gynecologists collected the cervical exfoliated cells from eligible women for p16/Ki-67 dual staining, LBC testing and HPV DNA testing. Histology diagnosis were used as gold standard. Sensitivities, specificities, positive predictive values (PPVs), negative predictive values (NPVs) of p16/Ki-67 dual staining, LBC testing and HPV16/18 testing for triage of HR-HPV positive population were calculated and compared. Results: A total of 295 HR-HPV infected women were selected, and the mean age was (44.29±11.48) years old. Positive rates of p16/Ki-67 dual staining, HPV16/18 testing and LBC testing were 70.17% (207), 56.95% (168) and 85.76% (253), respectively. When CIN2+as the endpoint, among the three triage methods, sensitivity of p16/Ki-67 dual staining was 90.00% (95%CI: 85.06%-93.43%), higher than the value of HPV 16/18 testing, but lower than the value of LBC testing. Specificity, PPV and NPV of p16/Ki-67 dual staining were the highest [71.58% (95%CI: 61.81%-79.67%), 86.96% (95%CI:81.69%-90.88%) and 77.27% (95%CI: 67.49%-84.78%)]. When detection for CIN3+, sensitivity of p16/Ki-67 dual staining was 92.90% (95%CI: 87.74%-95.99%), lower than the value of LBC testing, but higher than the value of HPV16/18 testing. Specificity of p16/Ki-67 dual staining was 55.00% (95%CI: 46.74%-63.00%), lower than the value of HPV16/18 testing, but higher than the value of LBC testing. PPV of p16/Ki-67 dual staining was 69.57% (95%CI: 62.99%-75.43%), lower than the value of HPV 16/18 testing, but higher than the value of LBC testing. NPV of p16/Ki-67 dual staining was 87.50% (95%CI: 78.99%-92.87%), higher than value of HPV 16/18 testing, but lower than the value of LBC testing. Conclusion p16/Ki-67 dual staining has better clinical effects than HPV 16/18 testing and LBC testing for triage women with HR-HPV infection.

Objective: To understand the needs of primary and secondary school students, parents and teachers for comprehensive sexuality education content, and to explore the applicability of comprehensive sexuality education content in China. Methods: By using the method of stratified cluster sampling, 5 571 primary and middle school students and 4 835 parents from Beijing, Chongqing, Heilongjiang (Harbin and Jiamusi) and Hubei (Wuhan and Xiaogan) were selected for questionnaire survey. Depending on the physical health monitoring points of various provinces and cities, 176 sexuality education related teachers in nine regions were surveyed. The development of the questionnaire was based on the content framework of comprehensive sexuality education proposed in the International Technical Guidelines for Sexuality Education. Results: Students had a higher demand for knowledge on the concepts of "relationship" (53.4% for primary school students, 57.6% for junior high school students) and "health and well-being skills" (71.1% for primary school students, 55.9% for junior high school students). Parents had higher demand for most comprehensive sexuality education content with the exception of lower need for "sex and sexual behavior (74.7% of primary school parents, 80.8% of junior high school parents)" and "sexual and reproductive health (primary parents 74.3%, junior high school parents 75.8%)". Teachers had a high demand for most comprehensive sexuality education content, but primary school teachers had a lower need for "values, rights, culture and sex(65.9%)", "sex and sexuality(60.1%)" and "sexual and reproductive health(66.8%)". The results of correspondence analysis showed that junior middle school teachers had the highest demand for comprehensive education content, followed by primary school parents, junior middle school parents, primary school teachers, junior middle school students and primary school students. Conclusion Teachers and parents have a higher demand for most comprehensive sexuality education content, while lower demand for some relatively sensitive content. Students have a relatively low demand for comprehensive sexuality education content. It is recommended to further improve the content of school sexuality education based on the actual needs of China.

Objective: To understand the awareness of sexual knowledge among primary and secondary school students, as well as their attitudes towards school sex education and to provide a reference for school-based sex education. Methods: A total of 5 531 primary and secondary school students were selected from Beijing, Chongqing, Heilongjiang (Harbin and Jiamusi) and Hubei (Wuhan and Xiaogan) by stratified cluster sampling and were investigated with self-reported questionnaire. Results: Sex-related knowledge scored 62.33±19.35, with a pass rate of 61.9%, and the excellent rate 17.5%. Sexual knowledge of junior high school students scored 64.30±19.07, with the pass rate of 64.7%, and the excellent rate 22.0%. A large proportion of primary students reported unaware of pubertal growth and HIV/AIDS-related knowledge, and a large proportion of junior high students unaware of reproductive physiology. Multivariate Logistic regression analysis showed region, grade, gender, and suburban areas were related to the level of sexual knowledge(P<0.05). More than 76.0% primary students and 85.9% middle school students agreed on the importance of school sexuality education. 59.7% of primary school students and 73.3% of junior high school students hoped to include school sexuality education in compulsory education courses; 59.8% of primary school students and 68.3% of junior high school students felt that school sexuality education should be equipped with specialized teachers. Conclusion Chinese primary and secondary school students are lack of sexual knowledge. As students have strong wills to get more information on sexual knowledge, schools should provide them with more comprehensive and effective sex education through appropriate courses and activities.

Objective The method for detecting expression of human CDK14 gene with Real-time quantitative PCR was developed.Methods To establish a method for detecting expression of human CDK14 gene with Real-time quantitative PCR by designing and synthesis of the primers of CDK14 target gene andβ-Actin reference gene and extracting total RNA from different lung cancer cell lines.Then the specificity,detection range and repeatability of this method were evaluated.At last,the expression level of CDK14 gene in different cell lines,which were with or without siRNA interference,were carried out by using this method.Results The method for detecting expression of human CDK14 gene with Real-time quantitative PCR,which had good specificity,good repeatability (CV=7.3 ％) and wide detection range (Ct value range of CDK14 and β-Actin amplification curve were 22.47～32.96 and 15.14～ 27.55 respectively,r2 =0.9844),was developed and it was verified by electrophoresis analysis,melting curve,PCR product sequencing.And CDK14 gene expression level,which was detected by this method,increased in HCC827 D5,H1650 and number 1 siRNA segment was effective interference segment.Conclusion The method for detecting expression of human CDK14 gene with Real-time quantitauve PCR was established successfully.

Objective To investigate the effect and significance of hyperbaric oxygenation in down-regulation of platelet membrane glycoproteins CD31 and CD62p in rats of traumatic brain injury (TBI).Methods Fifty-six SD rats were randomly distributed into TBI group,hyperbaric oxygenation group,and sham group by the lottery method.Furthermore,TBI group and hyperbaric oxygenation group were subgrouped at 6,48,and 96 hours.There were 8 rats per group.The rat models of severe TBI were induced by lateral fluid percussion.Levels of CD31 and CD62p were measured in all groups by flow cytometry.Results At 6,48 and 96 hours,expressions of CD31 (30.8 ± 8.9,32.5 ± 9.2 and 29.0 ±5.0) and CD62p (34.5 ±9.1,33.9 ±7.5 and 30.4 ±6.4) in TBI group were significantly higher than those (18.9-± 5.5,19.5 ± 6.1) in sham group (P ＜ 0.05).At 96 hours,expression of CD31 (22.7 ±5.5) in hyperbaric oxygenation group was significantly lower than 29.0 ± 5.0 in the TBI group (P ＜0.05).At 48 and 96 hours,expressions of CD62p (26.1 ± 5.8,23.6 ± 5.7) in hyperbaric oxygenation group were significantly lower than 33.9 ± 7.5 and 30.4 ± 6.4 in TBI group (P ＜ 0.05).Conclusions Platelet activation is enhanced in the acute phase after TBI.But platelet activation may be relieved with hyperbaric oxygenation,which is conducive to inhibiting microthrombosis and mitigating secondary brain injury after TBI.

Objective To observe the effects of hyperbaric oxygen (HBO) on the level of IL-1β and IL-10 in the lung tissue of mice following cerebral ischemia-reperfusion,and also to investigate their role and significance.Methods Adult male BALB/c mice were randomly divided into the ischemia-reperfusion group (or the IR group),the HBO group and the sham group.Cerebral ischemia-reperfusion model was established by clamping bilateral carotid arteries of mice.Level of IL-1β and IL-10 in the lung tissue at h 4,24 and 48 were measured respectively after cerebral ischemia-reperfusion by ELISA.Results The levels of IL-1β in the lung tissue of mice in the hyperbaric oxygen group and the ischemia-reperfusion group at h 4 were significantly higher than that of the sham group at the same time point (P ＜0.01).No statistical significance in the IL-1β levels could be seen in the lung tissue of mice between the HBO group and the ischemia-reperfusion group.The levels of IL-1β at h 24 for the HBO group and the ischemia-reperfusion group were all significantly higher than that of the sham group at the same time point (P ＜0.05,P ＜0.01).The level of IL-1β in the lung tissue of mice for the HBO group was significantly lower than that of the IR group at the same time point (P ＜ 0.05).The level of IL-10 in the lung tissue of the HBO group was significantly higher than those of the IR group and the sham group (P ＜ 0.05).Conclusions HBO could decrease the level of IL-1β and increase the level of IL-10 in the lung tissue of mice following cerebral ischemia-reperfusion,then it could offer some protection for acute lung injury induced by cerebral ischemia-reperfusion.

Objective To observe the effects of hyperbaric oxygen (HBO) on the level of IL-1β and IL-10 in the lung tissue of mice following cerebral ischemia-reperfusion,and also to investigate their role and significance.Methods Adult male BALB/c mice were randomly divided into the ischemia-reperfusion group (or the IR group),the HBO group and the sham group.Cerebral ischemia-reperfusion model was established by clamping bilateral carotid arteries of mice.Level of IL-1β and IL-10 in the lung tissue at h 4,24 and 48 were measured respectively after cerebral ischemia-reperfusion by ELISA.Results The levels of IL-1β in the lung tissue of mice in the hyperbaric oxygen group and the ischemia-reperfusion group at h 4 were significantly higher than that of the sham group at the same time point (P ＜0.01).No statistical significance in the IL-1β levels could be seen in the lung tissue of mice between the HBO group and the ischemia-reperfusion group.The levels of IL-1β at h 24 for the HBO group and the ischemia-reperfusion group were all significantly higher than that of the sham group at the same time point (P ＜0.05,P ＜0.01).The level of IL-1β in the lung tissue of mice for the HBO group was significantly lower than that of the IR group at the same time point (P ＜ 0.05).The level of IL-10 in the lung tissue of the HBO group was significantly higher than those of the IR group and the sham group (P ＜ 0.05).Conclusions HBO could decrease the level of IL-1β and increase the level of IL-10 in the lung tissue of mice following cerebral ischemia-reperfusion,then it could offer some protection for acute lung injury induced by cerebral ischemia-reperfusion.

Objective To investigate the effects of propofol and sevoflurane on oxidative stess response induced by short period pure oxygen inhalation during general anesthesia.Methods Sixty ASA Ⅰ or Ⅱ patients aged 20-60 yr weighing 50-85 kg undergoing elective abdominal surgery under general anesthesia were randomly divided into 2 groups (n=30 each):group propofol (group P) and group sevoflurane (group S).Each group was further divided into 2 subgroups inhaling 40% O2 (P0.4,S0.4) and 100%O2(P1.0,S1.0) respectively during operation.Anesthesia was induced with propofol 1-2 mg/kg,midazolan 0.02 mg/kg and sufentanil 0.1-0.2 mg/kg.Tracheal intobation was facilitated with rocuronium 0.6-0.8 mg/kg.The patients were mechanically ventilated(VT 8 ml/kg,RR 12 bpm).PET CO2 was maintained at 35-40 mmHg.Anesthesia was maintained with in both groups.BIS was maintained at 40-60.Arterial blood samples were collected immediately before induction of anesthesia (baseline),at 2,4,6h after tracheal intubation(T1-3) and 24h after operation(T4) for determination of PaO2,serum 8-iso-PGF2α and MDA concentrations and SOD activity.PaO2/FiO2 was calculated.Results In subgroup S1.0 the serum 8-iso-PGF2α and MDA concentrations were significantly increased while serum SOD activity was significanfly decreased at T1-3 as compared with the baseline.Serum 8-iso-PGF2α and MDA concentrations were significantly higher while serum SOD activity and PaO2/FiO2 were significantly lower at T1-3 in subgroup S1.0 than in stress response induced by≤6h pure O2 inhalation but inhalation of 1.5%-3.0% sevoflurane can not.