Objective: To analyze the changes and related factors of influenza vaccine hesitancy among college students in Shanghai from 2022 to 2025, so as to provide evidence for influenza prevention and control in universities. Methods: A stratified random cluster sampling method was used to select 1 393 college students from Shanghai universities for electronic questionnaire surveys in June 2022 and January to February 2025. The survey mainly included influenza vaccination status, sources of vaccine related information, and potential related factors. Data were analyzed using the Chi-square test, Mann-Whitney U test, and binary Logistic regression. Results: The influenza vaccine hesitancy rate among college students in Shanghai was higher in 2025 ( 40.63 %) than in 2022 (31.95%) ( χ 2=22.73), and the hesitancy score [2022:0 (0,5);2025:0 (0,5)] also increased ( Z = -4.16 ) (both P <0.01). The proportion of students exposed to reports of vaccine adverse events rose from 36.90% in 2022 to 59.22 % in 2025 ( χ 2=139.08, P <0.01). Binary Logistic regression analysis showed that basic influenza knowledge (2022: OR = 0.79 , 95% CI =0.68-0.92; 2025: OR =0.79, 95% CI =0.69-0.90) and previous self paid vaccination with other vaccines (2022: OR =0.52, 95% CI =0.37-0.75; 2025: OR =0.63, 95% CI =0.48-0.83) were associated with influenza vaccine hesitancy among college students in both years; medical professional background ( OR =0.34, 95% CI =0.17-0.67) and trust in healthcare providers recommendations ( OR =0.31, 95% CI =0.11-0.86) were associated with influenza vaccine hesitancy among college students in 2025 (all P <0.05). Conclusions The phenomenon of influenza vaccine hesitancy among college students in Shanghai has intensified. Increased knowledge has failed to offset concerns arising from exposure to negative information. Medical background and recommendations from healthcare providers have become new related factors.

Dry eye disease (DED) is a prevalent and intractable ocular disease induced by a variety of causes. Elevated sphingomyelin (SM) levels and pro-inflammatory cytokines were detected on the ocular surface of DED patients, particularly in the meibomian glands. Sphingomyelin synthase 2 (SMS2), one of the proteins involved in SM synthesis, would light a novel way of developing a DED therapy strategy. Herein, we report the design and optimization of a series of novel thiophene carboxamide derivatives to afford 14l with an improved highly potent inhibitory activity on SM synthesis (IC_{50, SMS2} = 28 nmol/L). Moreover, 14l exhibited a notable protective effect of anti-inflammation and anti-apoptosis on human corneal epithelial cells (HCEC) under TNF-α-hyperosmotic stress conditions in vitro, with an acceptable ocular specific distribution (corneas and meibomian glands) and pharmacokinetics (PK) profiles (t _{1/2, cornea} = 1.11 h; t _{1/2, meibomian glands} = 4.32 h) in rats. Furthermore, 14l alleviated the dry eye symptoms including corneal fluorescein staining scores and tear secretion in a dose-dependent manner in mice. Mechanically, 14l reduced the mRNA expression of Tnf-α, Il-1β and Mmp-9 in corneas, as well as the proportion of very long chain SM in meibomian glands. Our findings provide a new strategy for DED therapy based on selective SMS2 inhibitors.

Objective:To develop a training content framework for hospice volunteers based on the competency model.Methods:This was a qualitative study. A draft of the training content framwork for hospice volunteers was first formed according to results of literature review and qualitative interviews based on the competency model. From December 2023 to March 2024, two rounds of Delphi consultation with 16 experts were conducted to develop the final version of the system, and the weights of each indicator were determined with analytic hierarchy process.Results:Among the 14 consulting experts who completed two rounds of consultation, including 10 females, all held intermediate or above professional titles, and their working years ranged from 10 to 34 years, covering fields of hospice care, nursing management, clinical nursing and nursing education. In two rounds of consultation the expert positive coefficient was 14/16 and 14/14, the expert authority coefficient was 0.882 and 0.893, and the Kendall coordination coefficient of all indicators was 0.109 and 0.161 ( P<0.01), the coefficient of variation was 0-0.31 and 0-0.19, respectively. The final version of the system consisted of 3 first-level indicators, 7 second-level indicators, 20 third-level indicators, and 55 specific training contents. The three first-level indicators were knowledge, skill, role and value, and the weight coefficients were 0.335, 0.313 and 0.352, respectively. Each judgment matrix was consistent. Conclusions:A training content system for hospice care volunteers has been developed in this study, which may be used for medical institutions and organizations to train hospice care volunteers.

Objective:To develop a training content framework for hospice volunteers based on the competency model.Methods:This was a qualitative study. A draft of the training content framwork for hospice volunteers was first formed according to results of literature review and qualitative interviews based on the competency model. From December 2023 to March 2024, two rounds of Delphi consultation with 16 experts were conducted to develop the final version of the system, and the weights of each indicator were determined with analytic hierarchy process.Results:Among the 14 consulting experts who completed two rounds of consultation, including 10 females, all held intermediate or above professional titles, and their working years ranged from 10 to 34 years, covering fields of hospice care, nursing management, clinical nursing and nursing education. In two rounds of consultation the expert positive coefficient was 14/16 and 14/14, the expert authority coefficient was 0.882 and 0.893, and the Kendall coordination coefficient of all indicators was 0.109 and 0.161 ( P<0.01), the coefficient of variation was 0-0.31 and 0-0.19, respectively. The final version of the system consisted of 3 first-level indicators, 7 second-level indicators, 20 third-level indicators, and 55 specific training contents. The three first-level indicators were knowledge, skill, role and value, and the weight coefficients were 0.335, 0.313 and 0.352, respectively. Each judgment matrix was consistent. Conclusions:A training content system for hospice care volunteers has been developed in this study, which may be used for medical institutions and organizations to train hospice care volunteers.

Objective To study the gradual-onset rate (GOR)mode on human centrifuge G-tolerance determination and the applications in pilot selection and training. Methods Twenty-five pilots were undertaken the G-tolerance examination under centrifuge GOR operating mode and completed high G centrifuge training at +8.0 Gz level for 10 s.The pilots were divided into excellent group (n=1 3),good group (n=7 )and mediocre group (n=5 )by the high G training result.The pilots, G-tolerance under GOR mode and the anti-G effect of performing HP maneuver were analyzed. Results Under GOR mode pilots, strained tolerance (GOR2 )was significantly higher than their relaxed tolerance (GOR1 )(t=1 4.420,P=0.000 ).The differences on GOR1 tolerance and HP maneuver anti-G effect were not significant among different groups (F=2.593,0.1 76,P>0.05).The GOR2 tolerance of excellent group was significantly higher than that of other 2 groups (P<0.05). There was no significant difference on GOR2 tolerance among groups (P>0.05). Conclusions The G-tolerance and the effect of performing anti-G straining maneuver could be precisely and conveniently evaluated with GOR mode.It is necessary to establish the G-tolerance examination method and assessment criterion under centrifuge GOR mode for the G tolerance determination and training of high performance fighter aircraft pilot.

Objective To study the gradual-onset rate (GOR)mode on human centrifuge G-tolerance determination and the applications in pilot selection and training. Methods Twenty-five pilots were undertaken the G-tolerance examination under centrifuge GOR operating mode and completed high G centrifuge training at +8.0 Gz level for 10 s.The pilots were divided into excellent group (n=1 3),good group (n=7 )and mediocre group (n=5 )by the high G training result.The pilots, G-tolerance under GOR mode and the anti-G effect of performing HP maneuver were analyzed. Results Under GOR mode pilots, strained tolerance (GOR2 )was significantly higher than their relaxed tolerance (GOR1 )(t=1 4.420,P=0.000 ).The differences on GOR1 tolerance and HP maneuver anti-G effect were not significant among different groups (F=2.593,0.1 76,P>0.05).The GOR2 tolerance of excellent group was significantly higher than that of other 2 groups (P<0.05). There was no significant difference on GOR2 tolerance among groups (P>0.05). Conclusions The G-tolerance and the effect of performing anti-G straining maneuver could be precisely and conveniently evaluated with GOR mode.It is necessary to establish the G-tolerance examination method and assessment criterion under centrifuge GOR mode for the G tolerance determination and training of high performance fighter aircraft pilot.

Objective To analyze and discuss the status of fighter pilots' centrifuge training,to investigate the pilots' G tolerance and to provide reference for training program improvement.Methods One thousand and one hundred ninety-nine fighter pilots' human centrifuge training data were collected and analyzed by cross-sectional study.Pilots were classified by the training purposes that were for aeromedical qualification and competitive assessment.The up-to-standard rates were compared upon the analysis of pilots' training performance.Results In 877 pilots for aeromedical qualification,594 completed the runs of 8 G for 10 s,106 completed 9 G for 10 s.The qualification rate was 79.1％.In 180 high performance fighter pilots selected for competitive assessment,141 completed the runs of 9 G for 10 s.The qualification rate was 78.3％.In 142 second generation fighter pilots selected for competitive assessment,140 completed the runs of 7 G for 10 s.The qualification rate was 98.6％ which was significantly greater than that of the pilots for aeromedical qualification (x 2 =31.141,P ＜ 0.01) and high performance fighter pilots selected for competitive assessment (x 2 =29.318,P＜0.01).There was no difference on qualification rate between the latter two groups (x2 =0.058,P ＞0.05).The incidence of G-LOC and A-LOC was 5.0％ and 2.7％ respectively.The incidences of G-LOC (x2 =4.983,P＜0.05) and A-LOC (x2 =7.528,P＜0.05) of the pilots selected for competitive assessment were significantly greater than those of the pilots for aeromedical qualification.In 1 199 pilots,only 2 pilots (0.17％) had severe acute lumbar sprain.Conclusions Advanced anti-G methods have been adopted in our centrifuge training.The incidences of G-LOC and spinal injury induced by centrifuge training are low.The training program still needs further development and the training effectiveness should be improved.

Objective To analyze and discuss the status of fighter pilots' centrifuge training,to investigate the pilots' G tolerance and to provide reference for training program improvement.Methods One thousand and one hundred ninety-nine fighter pilots' human centrifuge training data were collected and analyzed by cross-sectional study.Pilots were classified by the training purposes that were for aeromedical qualification and competitive assessment.The up-to-standard rates were compared upon the analysis of pilots' training performance.Results In 877 pilots for aeromedical qualification,594 completed the runs of 8 G for 10 s,106 completed 9 G for 10 s.The qualification rate was 79.1％.In 180 high performance fighter pilots selected for competitive assessment,141 completed the runs of 9 G for 10 s.The qualification rate was 78.3％.In 142 second generation fighter pilots selected for competitive assessment,140 completed the runs of 7 G for 10 s.The qualification rate was 98.6％ which was significantly greater than that of the pilots for aeromedical qualification (x 2 =31.141,P ＜ 0.01) and high performance fighter pilots selected for competitive assessment (x 2 =29.318,P＜0.01).There was no difference on qualification rate between the latter two groups (x2 =0.058,P ＞0.05).The incidence of G-LOC and A-LOC was 5.0％ and 2.7％ respectively.The incidences of G-LOC (x2 =4.983,P＜0.05) and A-LOC (x2 =7.528,P＜0.05) of the pilots selected for competitive assessment were significantly greater than those of the pilots for aeromedical qualification.In 1 199 pilots,only 2 pilots (0.17％) had severe acute lumbar sprain.Conclusions Advanced anti-G methods have been adopted in our centrifuge training.The incidences of G-LOC and spinal injury induced by centrifuge training are low.The training program still needs further development and the training effectiveness should be improved.

The BCL6 (B-Cell Lymphoma 6) gene is a proto-oncogene that is often expressed in diffuse large B-cell lymphomas (DLBCLs). BCL6 loss of function can kill DLBCL cells, demonstrating that BCL6 is necessary for the survival of DLBCL cells and could be a therapeutic target. In this study, we found that BCL6 protein levels were consistently upregulated in DLBCL tissues, whereas its mRNA levels varied randomly in tissues, suggesting that a post-transcriptional mechanism was involved in BCL6 regulation. We used bioinformatics analysis to search for miRNAs, which potentially target BCL6, and identified specific targeting sites for miR-10a in the 3'-untranslated region (3'-UTR) of BCL6. We further identified an inverse correlation between miR-10a levels and BCL6 protein levels, but not mRNA levels, in DLBCL tumor tissue samples. By overexpressing or knocking down miR-10a in DLBCL cells, we experimentally validated that miR-10a directly recognizes the 3'-UTR of the BCL6 transcript and regulated BCL6 expression. Furthermore, we demonstrated that negatively regulating BCL6 by miR-10a suppressed the proliferation and promoted apoptosis of DLBCL cells.
3' Untranslated Regions ; Apoptosis ; Cell Line, Tumor ; Cell Proliferation ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; Humans ; Lymphoma, Large B-Cell, Diffuse ; genetics ; metabolism ; therapy ; MicroRNAs ; genetics ; metabolism ; Proto-Oncogene Proteins c-bcl-6 ; biosynthesis ; genetics

The isolated working heart of guinea pig perfused with Tyrode's solution could work normally for at least 60 min. It was shown that berbamine ( BA ) could depress the function of isolated working heart of guinea pig in dose-dependent manner. BA 3 mol/L could decrease the left ventricular pressure, aortic pressure, -dP/dtmax, aortic blood flow and coronary blood flow, and increase left ventricular end-diastolic pressure. BA 100 mol/L could result in the ventricular asystole, however, no obvious influence the contraction of atrium.It was also demonstrated that BA could antagonise the arrhythmias induced by the adrenaline in isolated working heart of guinea Pig.