Perimenopausal depression （PMD） is an affective disorder that occurs in women during the transition from sexual maturity to old age. It can induce various complications， such as insomnia and cognitive decline. The etiology of PMD is complex. Although multiple hypotheses have been proposed， there is still no unified theory that fully explains its pathogenesis. Research into its mechanisms relies heavily on animal experiments， and establishing reliable animal models is crucial for experimental studies. Appropriate animal models can better simulate human pathophysiological states， rapidly evaluate the efficacy and safety of drugs and intervention methods， grasp the essence of the disease， and uncover its intrinsic connections， thereby exploring more advanced intervention strategies. However， there is a lack of systematic review and summarization of literature related to model construction. Additionally， traditional Chinese medicine （TCM）， adhering to the principles of ''syndrome differentiation and treatment'' and ''holistic concept''， has shown significant efficacy in treating PMD. In recent years， research exploring and analyzing its therapeutic mechanisms has been increasing. Therefore， to gain a clearer and more comprehensive understanding of PMD animal modeling methods and the mechanisms of TCM， this paper reviewed Chinese and English literature on PMD animal models and mechanisms of TCM in PMD treatment. It summarized the construction methods of single-factor and multi-factor PMD models， and discussed the advantages and disadvantages of each modeling approach. Furthermore， it delved into the mechanisms of TCM intervention in PMD， revealing that TCM formulas primarily exert their effects by regulating the hypothalamic-pituitary-adrenal axis， hypothalamic-pituitary-ovarian axis， gut-brain axis， cell signaling pathways， neural circuits， hormone levels， and neurotransmitter levels. This review aims to provide a reference for future research in this field. In summary， by summarizing the progress in the methods for PMD animal model construction and the mechanisms of TCM， the paper seeks to offer new insights into the mechanistic research of TCM intervention in PMD.

Objective To explore the feasibility and reference value of allogeneic lung transplantation and postoperative monitoring in miniature pigs for lung transplantation research. Methods Two miniature pigs (R1 and R2) underwent left lung allogeneic transplantation. Complement-dependent cytotoxicity tests and blood cross-matching were performed before surgery. The main operative times and partial pressure of arterial oxygen (PaO₂) after opening the pulmonary artery were recorded during surgery. Postoperatively, routine blood tests, biochemical blood indicators and inflammatory factors were detected, and pathological examinations of multiple organs were conducted. Results The complement-dependent cytotoxicity test showed that the survival rate of lymphocytes between donors and recipients was 42.5%-47.3%, and no agglutination reaction occurred in the cross-matching. The first warm ischemia times of D1 and D2 were 17 min and 10 min, respectively, and the cold ischemia times were 246 min and 216 min, respectively. Ultimately, R1 and R2 survived for 1.5 h and 104 h, respectively. Postoperatively, in R1, albumin (ALB) and globulin (GLB) decreased, and alanine aminotransferase increased; in R2, ALB, GLB and aspartate aminotransferase all increased. Urea nitrogen and serum creatinine increased in both recipients. Pathological results showed that in R1, the transplanted lung had partial consolidation with inflammatory cell infiltration, and multiple organs were congested and damaged. In R2, the transplanted lung had severe necrosis with fibrosis, and multiple organs had mild to moderate damage. The expression levels of interleukin-1β and interleukin-6 increased in the transplanted lungs. Conclusions The allogeneic lung transplantation model in miniature pigs may systematically evaluate immunological compatibility, intraoperative function and postoperative organ damage. The data obtained may provide technical references for subsequent lung transplantation research.

Objective:To explore the effect of Sugammadex sodium on muscle relaxant recovery and coagulation function after modified radical mastectomy for breast cancer.Methods:A total of 110 patients who underwent modified radical mastectomy in Shanxi Cancer Hospital from May. 2022 to May. 2024 were chosen and separated into two groups according to random number table method, with 55 cases in each group, receiving the same anesthesia scheme. Muscle relaxant was monitored by train-of-four stimulation (TOF) . After surgery, when the TOF count was ≥2, the study group was given Sugammadex sodium, and the control group was given neostigmine + atropine. Operation-related indexes and adverse reactions were recorded; The muscle relaxant residue was analyzed at 5, 15 and 30min after injection of muscle relaxant antagonists; Blood clot formation time (K value) , blood clot formation rate (Angle) , maximum blood clot intensity (MA value) and blood clot reaction time (R value) of the two groups were compared before (T1) and 5min (T2) , 15min (T3) and 30min (T4) after injection.Results:The extubation time and the first extubation time in the study group were shorter [ (4.10±1.05) min, (22.95±2.24) h vs. (13.28±2.96) min, (29.83±3.06) h] ( t=21.68, 13.46, all P < 0.05) . The muscle relaxation residual rates in the study group at 5min and 15min were lower [12.73% (7/55) , 0.00% (0/55) vs. 100.00% (55/55) , 63.64% (35/55) ] ( χ2=85.16, 51.33, P < 0.05) . There were no differences in K value, Angle value, MA value and R value between the two groups at T1, T2, T3 and T4 ( t K=0.17, 0.44, 0.14, 0.13, t Angle=0.93, 1.29, 1.04, 1.00, t MA=1.17, 1.72, 0.87, 1.03, t R=0.36, 0.08, 0.09, 0.37, all P > 0.05) . The incidence rates of postoperative nausea, vomiting and respiratory depression in the study group were lower [5.45% (3/55) , 7.27% (4/55) , 0.00% (0/55) vs. 18.18% (10/55) , 21.82% (12/55) , 10.91% (6/55) ] ( χ2/ Fisher=4.27, 4.68, -, all P < 0.05) . Conclusion:Sugammadex sodium can promote the recovery of muscle relaxant in patients after modified radical mastectomy for breast cancer, and has little effect on coagulation function, which can reduce the occurrence of postoperative adverse reactions.

To summarize the perioperative nursing experience of a patient with thoracic and abdominal aortic dissection aneurysm after Stanford type A aortic dissection surgery,who underwent intra-abdominal aortic aneurysm endovascular isolation,in situ fenestration branch covered stent placement,and artificial blood vessel bypass.The nursing points include regular evaluation of the intra-abdominal pressure of the patient,being on guard against the rupture of the dissection;specialist nurses participated in the preoperative multidisciplinary discussion;establishing an emergency plan for the rescue of ruptured aortic dissection;monitoring the vital signs of patients closely to prevent critical complications during surgery.After multidisciplinary treatment and meticulous care,the patient's surgery went smoothly and the postoperative recovery was good.The patient was discharged 8 days later.

Objective To investigate the impact of respiratory colonization of carbapenem-resistant Acinetobacter baumannii(CRAB)on 28-day mortality of patients with severe/critical coronavirus disease 2019(COVID-19)in in-tensive care unit(ICU).Methods Clinical data were collected from severe/critical COVID-19 patients admitted to general ICU and respiratory ICU in a tertiary Chinese medicine hospital from December 9,2022 to December 9,2023.According to the detection results of CRAB from sputum culture during ICU stay,patients were divided into respiratory CRAB colonization group and non-CRAB colonization group.Kaplan-Meier survival curve was plotted to compare the 28-day mortality between two groups of patients after ICU admission.Relationship between respiratory CRAB colonization and 28-day mortality was explored by Cox proportional hazards model,and subgroup analysis was performed.Results A total of 88 patients with severe/critical COVID-19 were included in analysis,out of which 27 had respiratory CRAB colonization(CRAB colonization group)and 61 didn't have respiratory CRAB colo-nization(non-CRAB colonization group);37 patients(42.0％)died within 28 days after admission to the ICU.Ka-plan-Meier survival curve showed that the 28-day mortality in CRAB group was higher than that in non-CRAB group(66.7％vs 31.1％,P＜0.001).Cox univariate analysis showed that the 28-day mortality risk of patients in the CRAB colonization group was 2.91 folds of those in the non-CRAB colonization group(HR=2.91,95％CI:1.52-5.58;P＜0.05).After covariates adjusting for age,sex,underlying diseases,white blood cell count,neutrophil-to-lymphocyte ratio,hypersensitive C-reactive protein,respiratory frequency,oxygenation index,lactate dehydro-genase,urea nitrogen,creatinine,lactic acid,and D-dimer,the risk of death in the CRAB colonization group was still higher than that in the non-CRAB colonization group(HR=2.54,95％CI:1.15-5.58;P＜0.05).Subgroup analysis showed that respiratory CRAB colonization and 28-day mortality in severe/critical COVID-19 ICU patients was affected by gender,serum albumin,creatinine,and D-dimer levels(interaction P＜0.05 for all).Conclusion The short-term mortality of severe/critical COVID-19 patients in the ICU increases significantly after secondary colo-nization of respiratory CRAB.Effective prevention and control of colonization and infection by multidrug-resistant organisms are crucial for ensuring patient prognosis.

Objective To evaluate the efficacy and safety of different surgical approaches for extracting high-positioned supernumerary teeth in the maxillary anterior midline region.Three surgical methods,the labial approach,the palatal approach,and a longitudinal incision beside the upper lip frenum were compared.Methods A total of 102 patients were recruited from the Department of Oral and Maxillofacial Surgery at the Affiliated Stomatological Hospital of Anhui Medical University between January and September 2024.They were randomly assigned via a random-digit table to three groups:the labial approach group(n=32),the palatal approach group(n=34),and the longitudinal incision beside the upper lip frenum group(n=36).Operation time,bone removal volume,post-operative midfacial swelling,degree of pain,and patient satisfaction across the three groups were recorded and compared.Results The average operation time in the labial approach group((20.50±3.02)min)and the longitudinal incision group((21.66±2.31)min)were both significantly shorter than that in the palatal approach group((29.19±3.40)min).In contrast,the palatal approach group demonstrated markedly lower postoperative swelling and pain scores(P＜0.05),along with the highest patient satisfaction,as reflected in the OHIP-14 scale.However,the palatal approach required the greatest bone removal volume,measuring(91.19±9.86)mm3.Conclusion Despite the palatal approach yielding superior outcomes in terms of postoperative pain control,swelling reduction,and patient satisfaction,it necessitated longer operation time and involved greater bone removal.Meanwhile,the labial approach and the longitudinal incision beside the upper lip frenum were comparable in all measured parameters,showing no statistically significant differences.Therefore,when extracting high midline impacted teeth in the maxillary anterior region,the choice of surgical approach should balance safety,minimal invasiveness,and patient satisfaction.

Temporomandibular joint disc (TMJD) is a critical anatomical structure within the maxillofacial region. However, its self-repair ability is limited, which often leads to functional degradation. The development of tissue engineering technology provides a new treatment strategy for TMJD repair, in which the scaffold provides basic physical support and suitable cellular microenvironment. Scaffold materials include naturally derived scaffolds, decellularised extracellular matrix scaffolds and synthetic polymer scaffolds. Currently, advancements in material composite techniques, surface modification, and chemical modification, as well as the optimization of electrospinning and three-dimensional printing technologies, have significantly enhanced the overall performance of the scaffold. These improvements exhibit promising potential for the repair of TMJD with varying degrees of defects. This paper provides an overview of the progress in tissue engineering scaffold materials in repairing of TMJD, focusing on ideal biological properties, material types, scaffold optimization strategies and clinical application. It aims to serve as a reference for the development of scaffold materials and the clinical translation of tissue engineering.

Objective:To investigate the association between cardiovascular health(CVH) metrics and microvascular complications in patients with type 2 diabetes mellitus(T2DM).Methods:A cross-sectional study was conducted with 4 614 T2DM patients who received standardized metabolic disease management at the First Affiliated Hospital of Ningbo University from March 1st, 2018, to February 29th, 2024. Ideal CVH metrics were defined as non-smoking, non-drinking or moderate drinking, a healthy diet, regular physical activity, a healthy waist circumference, and adequate sleep duration. Microvascular complications included diabetic kidney disease(DKD), diabetic retinopathy(DR), and diabetic peripheral neuropathy(DPN). Logistic regression analysis was used to assess the relationship between CVH metrics and microvascular complications. Results:Patients with≤1, 2, 3, 4, or≥5 ideal CVH metrics were 378(8.2%), 933(20.2%), 1 480(32.1%), 1 218(26.4%), and 605(13.1%), respectively. A total of 2 736(59.3%) patients had at least one microvascular complication. Multivariate logistic regression analysis showed that compared to those with≤1 ideal CVH metric, patients with≥5 ideal CVH metric had an odds ratio( OR) of 0.55(95% CI 0.41-0.74, Ptrend<0.001) for microvascular complications, an OR of 0.51(95% CI 0.36-0.72, Ptrend<0.001) for DKD, and an OR of 0.54(95% CI 0.40-0.73, Ptrend<0.001) for DPN. No significant association was found for DR, with an OR of 1.31(95% CI 0.87-1.98, Ptrend=0.615). Conclusion:A great number of ideal CVH metrics was associated with a reduction in microvascular complications in T2DM patients, with variations across different types of microvascular complications.

Turning to critical illness is a common stage of various diseases and injuries before death. Patients usually have complex health conditions, while the treatment process involves a wide range of content, along with high requirements for doctor′s professionalism and multi-specialty teamwork, as well as a great demand for time-sensitive treatments. However, this is not matched with critical care professionals and the current state of medical care in China. Telemedicine, which shortens the distance of medical professionals and the gap of disease diagnosis and treatments in various regions through electronic information, can effectively solve the current problem. Therefore, there is an urgent need to develop a standardized, high-quality visualization telemedicine round system .Therefore, experts have been organized to search domestic and foreign literature on telemedicine round for critically ill patients and to form this consensus based on clinical experiences so as to further improve the level of critical care treatments in regions.

Objective To develop a virtual reality rehabilitation support system and evaluate its effects on improving discharge readiness in caregivers of laryngectomy patients.Methods The system is equipped with a virtual reality-based home nursing skills guidance module for post-laryngectomy patients and an evidence-based artificial intelligence Q&A module.A convenience sampling method was adopted to select 64 caregivers of patients who underwent laryngectomy for the first time in the otorhinolaryngology head and neck surgery department of a tertiary A hospital in Beijing from July 2024 to January 2025 for a systematic study on application effects.To avoid cross-contamination between an experimental group and a control group,ward-based grouping was applied.A coin toss was used to assign Ward 1 to an experimental group and Ward 2 to a control group,with 32 cases in each group.The experimental group received discharge guidance based on the virtual reality technology,while the control group was provided with conventional verbal and written discharge instructions.The readiness for hospital discharge and the quality of discharge teaching scores of caregivers were compared between the 2 groups after the intervention.Results No sample detachment.After the intervention,the patient caregivers in the experimental group scored significantly higher than those in the control group in terms of discharge readiness and quality of discharge guidance,with a statistically significant difference(P＜0.001).Conclusion This study developed the virtual reality rehabilitation system for laryngeal cancer patients demonstrated good feasibility and effectiveness.The application of this system for discharge education significantly improved discharge readiness of caregivers and quality of discharge guidance for post-laryngectomy patients.It also helped with the optimization of health education models,enhancement of nursing resource utilization efficiency,and improvement of transitional care.