Objective:To explore the clinicopathological features of rectal neuroendocrine tumor (R-NET) G2, identify prognostic factors, and summarize treatment experience.Methods:The clinical data of patients diagnosed with R-NET G2 by pathological diagnosis admitted to Cancer Hospital of the Chinese Academy of Medical Sciences from January 2003 to September 2023 were retrospectively analyzed. The Fisher's exact test and Kaplan-Meier curves were performed to analyze the association between pathological features and prognosis.Results:A total of 22 patients were enrolled in this study and 21 patients were followed up for a period of 6-98 months with a median follow-up time of 42 months. 5 patients died due to tumor progression during the follow-up period. The 1-, 3-, and 5-year cancer-specific survival (CSS) of the whole group were 100.0%, 92.9%, and 69.6%, respectively. Of the 22 patients, 20 underwent surgical treatment, of which 15 underwent postoperative adjuvant therapy; 2 underwent medical treatment for liver and bone multiple metastases. The 5-year survival rates of patients with tumours ≥2 cm in length, T2-3 stage, lymph node metastasis, and distant metastasis (57.1%, 68.8%, 66.7%, and 63.6%, respectively) were shorter than those of patients with tumours <2 cm in length, T1 stage, no lymph node metastasis, and no distant metastasis (all 100.0%, P＜0.001). In addition, patients with liver metastases had larger primary tumor diameters and higher T-stages compared with those without distant metastasis ( P＜0.05). Conclusions:R-NET G2 has a high degree of malignancy compared with G1 and a high propensity for metastasis. Clinicians should formulate appropriate diagnostic and treatment strategies based on factors such as tumor size, depth of invasion, lymph node status, presence of distant metastasis, and the location and extent of distant metastasis.

Temporomandibular joint disc (TMJD) is a critical anatomical structure within the maxillofacial region. However, its self-repair ability is limited, which often leads to functional degradation. The development of tissue engineering technology provides a new treatment strategy for TMJD repair, in which the scaffold provides basic physical support and suitable cellular microenvironment. Scaffold materials include naturally derived scaffolds, decellularised extracellular matrix scaffolds and synthetic polymer scaffolds. Currently, advancements in material composite techniques, surface modification, and chemical modification, as well as the optimization of electrospinning and three-dimensional printing technologies, have significantly enhanced the overall performance of the scaffold. These improvements exhibit promising potential for the repair of TMJD with varying degrees of defects. This paper provides an overview of the progress in tissue engineering scaffold materials in repairing of TMJD, focusing on ideal biological properties, material types, scaffold optimization strategies and clinical application. It aims to serve as a reference for the development of scaffold materials and the clinical translation of tissue engineering.

Temporomandibular joint disc (TMJD) is a critical anatomical structure within the maxillofacial region. However, its self-repair ability is limited, which often leads to functional degradation. The development of tissue engineering technology provides a new treatment strategy for TMJD repair, in which the scaffold provides basic physical support and suitable cellular microenvironment. Scaffold materials include naturally derived scaffolds, decellularised extracellular matrix scaffolds and synthetic polymer scaffolds. Currently, advancements in material composite techniques, surface modification, and chemical modification, as well as the optimization of electrospinning and three-dimensional printing technologies, have significantly enhanced the overall performance of the scaffold. These improvements exhibit promising potential for the repair of TMJD with varying degrees of defects. This paper provides an overview of the progress in tissue engineering scaffold materials in repairing of TMJD, focusing on ideal biological properties, material types, scaffold optimization strategies and clinical application. It aims to serve as a reference for the development of scaffold materials and the clinical translation of tissue engineering.

Objective To explore the application value of high-flow nasal oxygen therapy combined with non-invasive positive pressure ventilation in very low birth weight infants after weaning from invasive mechanical ventilation.Methods From February 2022 to May 2024,80 cases of very low birth weight infants who were weaned from invasive mechanical ventilation in Second Affiliated Hospital of Wenzhou Medical University were selected and divided into single ventilation group and combined oxygen therapy group according to the random number table method,with 40 cases in each group.Single ventilation group was intervened with non-invasive positive pressure ventilation,while combined oxygen therapy group was intervened with high-flow nasal oxygen therapy combined with non-invasive positive pressure ventilation.The disappearance time of clinical symptoms,the score of neonatal behavioral neurological assessment(NBNA),vitamin D and blood gas indicators of two groups of premature infants were compared.And the degree of broncho pulmonary dysplasia(BPD)in premature infants was evaluated.Results The disappearance time of clinical symptoms,non-invasive positive pressure ventilation time and total oxygen requirement time of preterm infants in combined oxygen therapy group were significantly shorter than those in single ventilation group(P＜0.05).After 14 days of intervention,the NBNA scores,vitamin D,oxygenation index and blood oxygen saturation of premature infants in both groups were significantly higher than those before intervention in this group(P＜0.05).The NBNA score,vitamin D,oxygenation index and blood oxygen saturation of preterm infants in combined oxygen therapy group were significantly higher than those in single ventilation group(P＜0.05).The degree of BPD in combined oxygen therapy group was significantly milder than that in single ventilation group(x2=8.571,P=0.003).Conclusion After weaning from invasive mechanical ventilation in very low birth weight infants,high-flow nasal oxygen therapy combined with non-invasive positive pressure ventilation intervention can help reduce inflammatory responses and brain damage,improve blood gas indicators,and lower the degree of BPD.

Objective To explore the application value of high-flow nasal oxygen therapy combined with non-invasive positive pressure ventilation in very low birth weight infants after weaning from invasive mechanical ventilation.Methods From February 2022 to May 2024,80 cases of very low birth weight infants who were weaned from invasive mechanical ventilation in Second Affiliated Hospital of Wenzhou Medical University were selected and divided into single ventilation group and combined oxygen therapy group according to the random number table method,with 40 cases in each group.Single ventilation group was intervened with non-invasive positive pressure ventilation,while combined oxygen therapy group was intervened with high-flow nasal oxygen therapy combined with non-invasive positive pressure ventilation.The disappearance time of clinical symptoms,the score of neonatal behavioral neurological assessment(NBNA),vitamin D and blood gas indicators of two groups of premature infants were compared.And the degree of broncho pulmonary dysplasia(BPD)in premature infants was evaluated.Results The disappearance time of clinical symptoms,non-invasive positive pressure ventilation time and total oxygen requirement time of preterm infants in combined oxygen therapy group were significantly shorter than those in single ventilation group(P＜0.05).After 14 days of intervention,the NBNA scores,vitamin D,oxygenation index and blood oxygen saturation of premature infants in both groups were significantly higher than those before intervention in this group(P＜0.05).The NBNA score,vitamin D,oxygenation index and blood oxygen saturation of preterm infants in combined oxygen therapy group were significantly higher than those in single ventilation group(P＜0.05).The degree of BPD in combined oxygen therapy group was significantly milder than that in single ventilation group(x2=8.571,P=0.003).Conclusion After weaning from invasive mechanical ventilation in very low birth weight infants,high-flow nasal oxygen therapy combined with non-invasive positive pressure ventilation intervention can help reduce inflammatory responses and brain damage,improve blood gas indicators,and lower the degree of BPD.

Objective:To explore the clinicopathological features of rectal neuroendocrine tumor (R-NET) G2, identify prognostic factors, and summarize treatment experience.Methods:The clinical data of patients diagnosed with R-NET G2 by pathological diagnosis admitted to Cancer Hospital of the Chinese Academy of Medical Sciences from January 2003 to September 2023 were retrospectively analyzed. The Fisher's exact test and Kaplan-Meier curves were performed to analyze the association between pathological features and prognosis.Results:A total of 22 patients were enrolled in this study and 21 patients were followed up for a period of 6-98 months with a median follow-up time of 42 months. 5 patients died due to tumor progression during the follow-up period. The 1-, 3-, and 5-year cancer-specific survival (CSS) of the whole group were 100.0%, 92.9%, and 69.6%, respectively. Of the 22 patients, 20 underwent surgical treatment, of which 15 underwent postoperative adjuvant therapy; 2 underwent medical treatment for liver and bone multiple metastases. The 5-year survival rates of patients with tumours ≥2 cm in length, T2-3 stage, lymph node metastasis, and distant metastasis (57.1%, 68.8%, 66.7%, and 63.6%, respectively) were shorter than those of patients with tumours <2 cm in length, T1 stage, no lymph node metastasis, and no distant metastasis (all 100.0%, P＜0.001). In addition, patients with liver metastases had larger primary tumor diameters and higher T-stages compared with those without distant metastasis ( P＜0.05). Conclusions:R-NET G2 has a high degree of malignancy compared with G1 and a high propensity for metastasis. Clinicians should formulate appropriate diagnostic and treatment strategies based on factors such as tumor size, depth of invasion, lymph node status, presence of distant metastasis, and the location and extent of distant metastasis.

Objective To establish a method to simultaneously determinate cobalt, nickel, arsenic, molybdenum, silver, cadmium and lead in blood and urine using inductively coupled plasma tandem mass spectrometry (ICP-MS/MS). Methods The blood samples were diluted 20 times with a mixed solution of nitric acid (0.10% V/V) and Triton X-100 (0.02% V/V). The urine samples were diluted 10 times with nitric acid (1.00% V/V). Yttrium-89, rhodium-103, and lutetium-175 were used as internal standards to decrease matrix interference, and either On-Mass mode or Mass-Shift mode was used to decrease mass spectrometry interference, with detection by ICP-MS/MS. Results The linear ranges of the seven elements were 0.100-10.000 μg/L, with the correlation coefficient >0.999 9. The detection limits for the seven elements in the blood and urine were 0.003-0.021 and 0.003-0.031 μg/L, respectively, and the minimum quantification limits were 0.009-0.064 and 0.009-0.094 μg/L, respectively. The recovery rates were 96.64%-102.90% and 96.34%-104.50% for the blood and urine, respectively. The within-run relative standard deviations (RSDs) were 0.32%-2.33% and 0.25%-2.31%, and the between-run RSDs were 1.07%-3.81% and 1.30%-3.62% for blood and urine, respectively. The samples were stable at 4 ℃ for at least 14 days. Conclusion ICP-MS/MS is a simple, sensitive, and accurate method for rapid detection of seven heavy metal elements in the blood and urine of occupational hazard-exposed workers.

Brain aging is closely related to cognitive decline,neurodegenerative diseases,and vascular dementia.Hydrogen sulfide(H2S)can delay brain aging by regulating protein homeostasis,anti-oxidative stress,inhibiting inflammation,reducing brain cell apoptosis and improving microcirculation.

The department of critical care medicine, the clinical base of critical care medicine, is the centralized management unit for the treatment of critical patients. Medicine and nursing are independent disciplines, but both can be fully utilized from different perspectives when dealing with critically ill patients. With the development of critical care medicine understanding and technology, the concept of doctor-nurse integration construction in the department of critical care medicine has emerged. Doctor-nurse integration construction in the department of critical care medicine needs to take severe diseases as the core and academic development as the orientation. Clinical practice should be carried out through medical cooperation, based on critical cognition and unified thinking mode.

The development, progression, and curative efficacy of head and neck squamous cell carcinoma (HNSCC) are influenced by complex interactions between epithelial and immune cells. Nevertheless, the specific changes in the nature of these interactions and their underlying molecular mechanisms in HNSCC are not yet fully understood. Cuproptosis, a form of programmed cell death that is dependent on copper, has been implicated in cancer pathogenesis. However, the understanding of cuproptosis in the context of HNSCC remains limited. In this study, we have discovered that cuproptosis-related long non-coding RNAs (CRLs) known as JPX play a role in promoting the expression of the oncogene urokinase-type plasminogen activator (PLAU) by competitively binding to miR-193b-3p in HNSCC. The increased activity of the JPX/miR-193b-3p/PLAU axis in malignant epithelial cells leads to enhanced cell proliferation, migration, and invasion in HNSCC. Moreover, the overexpression of PLAU in tumor epithelial cells facilitates its interaction with the receptor PLAUR, predominantly expressed on macrophages, thereby influencing the abnormal epithelial-immune interactome in HNSCC. Notably, the JPX inhibitor Axitinib and the PLAU inhibitor Palbociclib may not only exert their effects on the JPX/miR-193b-3p/PLAU axis that impacts the malignant tumor behaviors and the epithelial-immune cell interactions but also exhibit synergistic effects in terms of suppressing tumor cell growth and arresting cell cycle by targeting epidermal growth factor receptor (EGFR) and cyclin-dependent kinase (CDK4/6) for the treatment of HNSCC.
Humans ; MicroRNAs/metabolism* ; RNA, Long Noncoding/metabolism* ; Head and Neck Neoplasms/metabolism* ; Cell Proliferation ; Squamous Cell Carcinoma of Head and Neck/genetics* ; Urokinase-Type Plasminogen Activator/genetics* ; Cell Movement ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic ; Carcinoma, Squamous Cell/genetics* ; Neoplasm Invasiveness