Tuberculosis remains the leading cause of death from a single infectious agent worldwide.China is still one of the countries with a high burden of tuberculosis.The structural and functional damage to lung tissue caused by post-tuberculosis pulmonary disease(PTLD)imposes a considerable disease burden on people.How-ever,due to the insufficient understanding of post-tuberculosis lung diseases in the past,the long-term manage-ment of this disease has been lacking for the post-tuberculosis population.This article elaborates on the epidemiol-ogy and hazards,pathogenesis,clinical manifestations,diagnostic methods,treatment and preventive measures of common PTLD,with the aim of enhancing the awareness and attention of tuberculosis physicians to PTLD.It also summarizes the limitations in the etiological mechanism,diagnosis and treatment methods of PTLD,as well as the urgency of in-depth research.

Paraplegia caused by spinal cord injury is a serious neurological complication, for which surgery is currently the main treatment method. Due to different surgical approaches, patients are usually expected to maintain a passive prone position for a long time or switch between the supine and prone positions. Affected by multiple factors such as neurogenic sensory disorders, pathological changes in muscle tone and operative duration, the risk of intraoperative acquired pressure injury (IAPI) is significantly increased. Current clinical prevention strategies for IAPI in these patients predominantly focus on localized pressure relief during positioning, lacking systematic, standardized comprehensive prevention protocols or evidence-based guidelines. To address it, Department of Nursing, Orthopedics Branch, China International Exchange and Promotive Association for Medical and Health Care, Spinal Trauma Professional Committee, Orthopedics Branch, Chinese Medical Doctor Association, Nursing Group of Spine and Spinal Cord Professional Committee of Chinese Association of Rehabilitation Medicine organized experts in relevant fields to formulate Guideline for the prevention of intraoperative acquired pressure injury in paraplegic patients with spinal cord injury ( version 2025), based on evidence-based medical evidence and latest research results and clinical practice at home and abroad. Eleven recommendations were put forward from the aspects of preoperative risk assessment, intraoperative prevention strategies, postoperative handover and monitoring, and supportive mechanisms for IAPI prevention, aiming to standardize the prevention measures and management strategies of IAPI in paraplegic patients with spinal cord injury and accelerate the recovery of patients and improve the therapeutic effect.

Small colony variants(SCVs)are unique phenotypic variants produced by bacteria such as Staphylococcus aureus under environmental selective pressure,with specific biological characteristics,including slow growth,reduced pigment synthesis,auxotrophy,enhanced drug resistance,and easier intracellular colonization and biofilm formation.In recent years,it has been increasingly recognized that SCVs play a crucial role in the chronic progression of infections and poor prognosis.SCVs exhibit significant heterogeneity with complex and diverse molecular profiles.Compared with wild-type strains,SCVs have low virulence and significantly enhanced adherence,and they can effectively evade immune system recognition and clearance.SCVs invade host cells,including macrophages,and form dormant intracellular forms,causing antimicrobial resistance.These variants can revert to wild-type bacteria when environmental conditions improve,causing persistent and refractory infections such as osteomyelitis,cystic fibrosis,and implant-associated infections.However,current treatments for SCV-related infections are limited to long-term antibiotic therapy combined with debridement of infected tissue,and understanding of SCVs,their pathogenic mechanisms,and treatments remains limited.Traditional therapies,such as rifampicin combined with vancomycin,have limited efficacy against intracellular SCVs.Novel strategies,such as targeting ATP synthase inhibitors(eg.lycopene),using nanocarrier-delivered antibiotics to enhance intracellular penetration,alkalinizing of the microenvironment,or disrupting biofilms by physical therapies,are important breakthroughs in the fight against SCV-associated infections.This paper summarizes the biological characteristics,pathogenic mechanisms,and therapeutic progress of SCVs,providing reference for research and treatment of SCV-related infections.

Objective To investigate the population density and seasonal fluctuations of Aedes albopictus in Guangzhou City, Guangdong Province, from 2021 to 2023, so as to provide insights into A. albopictus control and management of dengue fever. Methods The surveillance of A. albopictus density was performed in all surveillance sites assigned across all streets (townships) in Guangzhou City during the period from January to December from 2021 to 2023. The surveillance frequency was twice every half month from May to September, and once every month for the rest of a year. In each surveillance period, A. albopictus mosquito larvae were captured from indoor and outdoor small water containers in residential areas, parks, medical facilities, schools, other government sectors and social organizations, construction sites, special industries and others for mosquito species identification. Adult mosquitoes were captured using electric mosquito suction apparatus for species identification and gender classification. Adult mosquitoes and mosquito eggs were collected with mosquito and egg traps at the breeding and dwelling places of Aedes mosquitoes for identification. The mosquito oviposition index (MOI), Breteau index (BI), adult mosquito density index (ADI) and standard space index (SSI) were calculated. The A. albopictus density was classified into grades 0, 1, 2 and 3 in each surveillance site, with Grade 0 density defined eligible, and the eligible rate of A. albopictus density was calculated at all surveillance sites each year from 2021 to 2023. In addition, the changing trends in MOI, SSI, BI and ADI of A. albopictus were analyzed in Guangzhou City from 2021 to 2023. Results The eligible rates of A. albopictus density were 61.69%, 68.75% and 55.15% in surveillance sites of Guangzhou City from 2021 to 2023 (χ² = 297.712, P < 0.001), and appeared a tendency towards a reduction followed by a rise each year, which gradually reduced since January, maintained at a low level during the period between May and October, and gradually increased from November to December. The MOI, SSI, BI and ADI of A. albopictus all appeared a tendency towards a rise followed by a reduction in Guangzhou City during the period between January and December from 2021 to 2023. The BI of A. albopictus peaked in the first half of June in 2021 (4.03), the first half of July in 2022 (3.89) and the last half of August in 2023 (5.02), and the SSI of A. albopictus peaked in the last half of June in 2021 (0.93), the last half of May in 2022 (0.59), and the last half of June (0.94) and the first half of September in 2023 (1.12). In addition, the MOI of A. albopictus peaked in the first half of May in 2021 (8.64), the first half of June in 2022 (8.96), and the last half of May (10.21) and the last half of June in 2023 (10.89), and the ADI of A. albopictus peaked in the first half of June in 2021 (3.41), the last half of June in 2022 (4.06), and the first half of July in 2023 (3.61). Conclusions The density of A. albopictus is high in Guangzhou City during the period from May to October, and the risk of local outbreak caused by imported dengue fever is high. Persistent intensified surveillance of the density and seasonal fluctuation of A. albopictus is recommended and timely mosquito prevention and control is required according to the fluctuation in the A. albopictus density.

Small colony variants(SCVs)are unique phenotypic variants produced by bacteria such as Staphylococcus aureus under environmental selective pressure,with specific biological characteristics,including slow growth,reduced pigment synthesis,auxotrophy,enhanced drug resistance,and easier intracellular colonization and biofilm formation.In recent years,it has been increasingly recognized that SCVs play a crucial role in the chronic progression of infections and poor prognosis.SCVs exhibit significant heterogeneity with complex and diverse molecular profiles.Compared with wild-type strains,SCVs have low virulence and significantly enhanced adherence,and they can effectively evade immune system recognition and clearance.SCVs invade host cells,including macrophages,and form dormant intracellular forms,causing antimicrobial resistance.These variants can revert to wild-type bacteria when environmental conditions improve,causing persistent and refractory infections such as osteomyelitis,cystic fibrosis,and implant-associated infections.However,current treatments for SCV-related infections are limited to long-term antibiotic therapy combined with debridement of infected tissue,and understanding of SCVs,their pathogenic mechanisms,and treatments remains limited.Traditional therapies,such as rifampicin combined with vancomycin,have limited efficacy against intracellular SCVs.Novel strategies,such as targeting ATP synthase inhibitors(eg.lycopene),using nanocarrier-delivered antibiotics to enhance intracellular penetration,alkalinizing of the microenvironment,or disrupting biofilms by physical therapies,are important breakthroughs in the fight against SCV-associated infections.This paper summarizes the biological characteristics,pathogenic mechanisms,and therapeutic progress of SCVs,providing reference for research and treatment of SCV-related infections.

Tuberculosis remains the leading cause of death from a single infectious agent worldwide.China is still one of the countries with a high burden of tuberculosis.The structural and functional damage to lung tissue caused by post-tuberculosis pulmonary disease(PTLD)imposes a considerable disease burden on people.How-ever,due to the insufficient understanding of post-tuberculosis lung diseases in the past,the long-term manage-ment of this disease has been lacking for the post-tuberculosis population.This article elaborates on the epidemiol-ogy and hazards,pathogenesis,clinical manifestations,diagnostic methods,treatment and preventive measures of common PTLD,with the aim of enhancing the awareness and attention of tuberculosis physicians to PTLD.It also summarizes the limitations in the etiological mechanism,diagnosis and treatment methods of PTLD,as well as the urgency of in-depth research.

Paraplegia caused by spinal cord injury is a serious neurological complication, for which surgery is currently the main treatment method. Due to different surgical approaches, patients are usually expected to maintain a passive prone position for a long time or switch between the supine and prone positions. Affected by multiple factors such as neurogenic sensory disorders, pathological changes in muscle tone and operative duration, the risk of intraoperative acquired pressure injury (IAPI) is significantly increased. Current clinical prevention strategies for IAPI in these patients predominantly focus on localized pressure relief during positioning, lacking systematic, standardized comprehensive prevention protocols or evidence-based guidelines. To address it, Department of Nursing, Orthopedics Branch, China International Exchange and Promotive Association for Medical and Health Care, Spinal Trauma Professional Committee, Orthopedics Branch, Chinese Medical Doctor Association, Nursing Group of Spine and Spinal Cord Professional Committee of Chinese Association of Rehabilitation Medicine organized experts in relevant fields to formulate Guideline for the prevention of intraoperative acquired pressure injury in paraplegic patients with spinal cord injury ( version 2025), based on evidence-based medical evidence and latest research results and clinical practice at home and abroad. Eleven recommendations were put forward from the aspects of preoperative risk assessment, intraoperative prevention strategies, postoperative handover and monitoring, and supportive mechanisms for IAPI prevention, aiming to standardize the prevention measures and management strategies of IAPI in paraplegic patients with spinal cord injury and accelerate the recovery of patients and improve the therapeutic effect.

Clinically, it is challenging to differentiate Crohn's disease (CD) from abdominal actinomycosis due to their similar symptoms. However, cases of CD with secondary actinomycosis infection are rare. This article systematically reviews the diagnosis and treatment process of a patient with CD complicated by secondary actinomycosis infection at the Air Force Medical University Xijing Digestive Disease Hospital, to provide ideas and experience for future clinical diagnosis and treatment.

Clinically, it is challenging to differentiate Crohn's disease (CD) from abdominal actinomycosis due to their similar symptoms. However, cases of CD with secondary actinomycosis infection are rare. This article systematically reviews the diagnosis and treatment process of a patient with CD complicated by secondary actinomycosis infection at the Air Force Medical University Xijing Digestive Disease Hospital, to provide ideas and experience for future clinical diagnosis and treatment.

Objective To explore the effective components and possible targets and mechanism of Shenling Baizhu Powder in improving adipose tissue inflammation in children with obesity with the help of network pharmacology and molecular docking method;To conduct preliminary verification through animal experiments.Methods The active components and targets of Shenling Baizhu Powder were screened through the TCMSP database.GeneCards,OMIM,DisGeNET databases were used to obtain the disease targets.An active component-target network was constructed by taking the intersection of drug and disease targets.Protein-protein interaction networks were constructed and core targets were obtained for GO and KEGG pathway enrichment analysis.Molecular docking validation was performed to key components and core targets.Animal experiments were conducted by establishing a model of young obese rats induced by high fat diet,and Shenling Baizhu Powder were given for gavage.The contents of TG and TC in serum and mRNA expressions of TNF-α,IL-1β,INF-γ and Fas in rat adipose tissue of epididymis were detected.Results The key components of Shenling Baizhu Powder to improve the microinflammation of obesity in children were piperlonguminine,acacetin,luteolin,denudatin B,mandenol,inermine,etc.There were 515 common drug-disease targets,and the core targets were TP53,SRC,PIK3R1,HSP90AA1 and PIK3CA.The results of GO enrichment analysis included inflammatory response,positive regulation of MAPK cascade,membrane raft,protein serine/threonine/tyrosine kinase activity,etc.KEGG pathway enrichment analysis results included metabolic,immune,endocrine,cancer and related liver disease signaling pathways.The results of molecular docking showed that the key components could play a regulatory role on the core target.Animal experiments showed that Shenling Baizhu Powder could improve microinflammation and metabolic indexes of model rats.Conclusion Shenling Baizhu Powder can act on multiple targets through various active components and multiple signaling pathways,adjusting inflammatory response and immune process,alleviating insulin resistance,regulating glucose and lipid metabolism,thereby improving adipose tissue inflammatory in children with obesity.