Objective To investigate the value of cranial CT cisternal grading combined with D-dimer(D-D)and Glasgow Coma Scale(GCS)score in predicting the short-term postoperative prog-nosis of patients with severe traumatic brain injury.Methods A total of 165 patients with severe trau-matic brain injury who were treated in the hospital from January 2019 to May 2024 were selected as study subjects,all underwent craniotomy surgery.Postoperative follow-up was conducted for 3 months to analyze the differences in clinical data and preoperative indicators such as cranial CT cisternal grad-ing,D-D levels,and GCS scores between patients with poor and good prognosis.The value of cranial CT cisternal grading,D-D levels,and GCS scores in predicting short-term postoperative poor prognosis in patients with severe traumatic brain injury was also analyzed.Results Compared with patients with good prognosis,patients with poor prognosis had higher proportion of age,cranial CT cisternal grading of Ⅰ to Ⅱ,D-D levels,and GCS scores＜6(P＜0.05).There were no statistically significant differences in C-reactive protein,prothrombin time,activated partial thromboplastin time,international normalized ratio,total cholesterol,triglycerides,high-density lipoprotein cholesterol,and low-density lipoprotein cholesterol levels between patients with poor and good prognosis(P＞0.05).Cranial CT cisternal grading,D-D levels,and GCS scores were influencing factors for short-term postoperative poor prognosis in patients with severe traumatic brain injury(P＜0.05).The area under the curve for poor prognosis by three indicators in combination was 0.941(95％CI,0.906 to 0.975),which was higher than the area under the curve for the individual predictions of cranial CT cisternal grad-ing,D-D levels,and GCS scores(P＜0.05).Conclusion The influencing factors for short-term postoperative prognosis in patients with severe traumatic brain injury include cranial CT cisternal grading,D-D levels,and GCS scores.The model based on these three indicators has certain appli-cation value in predicting patient prognosis.

Objective To analyze effects of Jinfeng pills combined with drospirenone and ethinylestradiol tablets on endometrial repair and hemodynamics in patients after artificial abortion.Methods A total of 106 patients who underwent artificial abortion in Ganzhou People's Hospital from July 2021 to July 2022 were selected.The patients were divided into Control group and observation group by a random number table method,53 cases in each group.Control group was treated with drospirenone and ethinylestradiol tablets after operation,and observation group was treated with drospirenone and ethinylestradiol tablets combined with Jinfeng pills after operation.The curative effect,traditional Chinese medicine(TCM)syndrome score,endometrial thickness and hemodynamics index were compared between two groups.Results After treatment,scores of TCM syndromes in observation group were lower than those in control group(P＜0.05).The total effective rate of observation group was higher than that of control group(P＜0.05).The postoperative vaginal bleeding time and the first menstrual time in observation group were shorter than those in control group,and postoperative vaginal bleeding volume was less than that in control group(P＜0.05).After treatment,uterine artery pulsatility index and resistance index in observation group were lower than those in control group,end-diastolic blood flow velocity and peak systolic velocity were higher than those in control group,and endometrial thickness was greater than that in control group(P＜0.05).The total incidence of postoperative complications in observation group was lower than that in control group(P＜0.05).Conclusion Jinfeng pills combined with drospirenone and ethinylestradiol tablets can effectively repair endometrium,promote hemodynamic improvement and reduce complications in patients with artificial abortion after operation.

Objective To analyze effects of Jinfeng pills combined with drospirenone and ethinylestradiol tablets on endometrial repair and hemodynamics in patients after artificial abortion.Methods A total of 106 patients who underwent artificial abortion in Ganzhou People's Hospital from July 2021 to July 2022 were selected.The patients were divided into Control group and observation group by a random number table method,53 cases in each group.Control group was treated with drospirenone and ethinylestradiol tablets after operation,and observation group was treated with drospirenone and ethinylestradiol tablets combined with Jinfeng pills after operation.The curative effect,traditional Chinese medicine(TCM)syndrome score,endometrial thickness and hemodynamics index were compared between two groups.Results After treatment,scores of TCM syndromes in observation group were lower than those in control group(P＜0.05).The total effective rate of observation group was higher than that of control group(P＜0.05).The postoperative vaginal bleeding time and the first menstrual time in observation group were shorter than those in control group,and postoperative vaginal bleeding volume was less than that in control group(P＜0.05).After treatment,uterine artery pulsatility index and resistance index in observation group were lower than those in control group,end-diastolic blood flow velocity and peak systolic velocity were higher than those in control group,and endometrial thickness was greater than that in control group(P＜0.05).The total incidence of postoperative complications in observation group was lower than that in control group(P＜0.05).Conclusion Jinfeng pills combined with drospirenone and ethinylestradiol tablets can effectively repair endometrium,promote hemodynamic improvement and reduce complications in patients with artificial abortion after operation.

OBJECTIVE：To establish the m ethod for simultaneous determination of 8 kinds of ginsenosides in Panax quinquefolium broken pieces. METHODS ：HPLC-DAD method was used to determine the contents of ginsenoside Rg 1，Re，Rb1， Rc，Ro，Rb2，Rb3，Rd in P. quinquefolium broken pieces. The determination was performed on Agilent 5 TC-C18 column with mobile phase consisted of acetonitrile- 0.2％ phosphoric acid water solution （gradient elution ）at the flow rate of 1.0 mL/min. The column temperature was set at 30 ℃. The detection wavelength was set at 203 nm，and sample size was 10 μL. Ginsenoside Re and ginsenoside Rb 2 were used as control ，liner calibration with two-reference substances correction was used to predict the retention time of other 6 components，and was compared with the relative retention time method. Using ginsenoside Re as control ， above components were quantified by the relative correction factor method ，and the results were compared with the external standard method. RESULTS ：The contents of ginsenoside Rg 1，Re，Rb1，Rc，Ro，Rb2，Rb3，Rd were 10.59-12.78，2.160-2.768， 27.492-38.880，3.154-4.018，3.368-4.080，0.343-0.755，0.961-1.415，5.857-6.923 mg/g. The accuracy of two-reference substances linear correction method to predict the retention time of components was higher ，and the absolute deviation of the predicted retention time was lower than that of the relative retention time method. There was no significant difference between the relative correction factor method and the external standard method ，and relative error was ＜3％ . CONCLUSIONS ：Established two-reference substances for determination of multiple components can be used for qualitative and quantitative analysis of 8 kinds of ginsensides in P. quinquefolium broken pieces simultaneously and accurately.

Objective To analyze the complications of adenoidectomy assisted with ablation under nasal endoscope. Method Two hundred and seventy-two cases of adenoidectomy assisted with ablation under nasal endoscope from April 2014 to April 2018 were retrospectively studied. The type, number, causes and processing methods of postoperative complications were analyzed. Results There were 67 cases (24.63％) with atlantoaxial rotatory deformity, 27 cases (9.93％) with fever, 11 cases (4.04％) with headache, 4 cases (1.47％) with postoperative recurrence, and 1 case (0.37％) with secondary postoperative haemorrhage. Conclusions The technology of adenoidectomy assisted with ablation under nasal endoscope is an efficient and minimally invasive method with less complications in the treatment of children adenoidectomy diseases. Atlantoaxial rotatory deformity is a common postoperative complication which is easy to be ignored by clinical doctors. The prognosis is good, after given a positive symptomatic treatment.

Objective To explore the application value of respiratory syncytial virus RNA (RSV-RNA) and respiratory syncytial virus IgM (RSV-IgM) antibody in the diagnosis of children with respiratory infection .Methods From September 2015 to April 2017 ,56 cases of respiratory syncytial virus infection were selected ,and the specimens of pharyngeal secretions were collected ,and RSV-RNA was detected ,in addition ,fasting venous blood was taken from children ,and the RSV-IgM antibody was detected .The positive rate of detection and correlation with age and time of onset were statistically analyzed .Results The positive rates of RSV-RNA and RSV-IgM were 89 .29% and 32 .14% ,respectively .The detection rate of RSV-RNA was significantly higher than that of RSV-IgM ,and the difference was statistically significant (P<0 .05) .The two groups ,≤6 months and from >6 months to 1 year old ,the detection rates of RSV-RNA in children was higher than RSV-IgM (P<0 .05) ,and the detection rate of RSV-RNA in chil-dren with ≤7 days of onset was higher than that of RSV-IgM (P<0 .05) .Conclusion The detection of children with RSV infec-tion ,which the detection of RNA has higher accuracy than IgM ,especially for children ≤1 year old ,the onset time is shorter(≤7 days) ,plays an important guiding role for early diagnosis .

Objective To evaluate effects of acitretin and interferon?α(INF?α)alone or in combination on the proliferative activity of and interleukin?15 expression in human cutaneous T?cell lymphoma Hut78 cells. Methods Cultured Hut78 cells were divided into several groups, including blank control group, negative control group, dimethyl sulphoxide (DMSO) group and experimental groups. Cells in experimental groups were additionally classified into several subgroups to be treated with acitretin(0.1-10μmol/L, acitretin groups)or INF?α(5 000-20 000 IU/ml, INF?αgroups) alone, or the combination of 1.0 μmol/L acitretin and IFN?α at concentrations of 5 000- 20 000 IU/ml (combination groups), for 24, 48 and 72 hours. Subsequently, cell counting kit 8(CCK8)assay was performed to assess the proliferative activity of Hut78 cells, and enzyme?linked immunosorbent assay(ELISA)to measure the expression of IL?15 in these cells. Results The proliferative activity of and IL?15 expression in Hut78 cells were both obviously suppressed in the acitretin groups and combination groups compared with the DMSO group, as well as in the INF?αgroups compared with the negative control group, and the inhibitory effects gradually increased with the increase in acitretin or INF?αconcentrations and treatment durations. As repeated measures analysis of variance revealed, there was a significant difference in both proliferation inhibition rates and IL?15 expression among different treatment durations and among different concentrations of acitretin or INF?α(all P<0.05), and there was an interaction effect between treatment durations and drug concentrations(all P<0.05). A significant difference was observed in both proliferation inhibition rates and IL?15 expression at 24, 48 and 72 hours when the 1.0?μmol/L acitretin+ 10 000/20 000?IU/ml IFN?αgroup was compared with the 1.0?μmol/L acitretin group and 10 000/20 000 IU/ml IFN?αgroup(all P<0.05). There was also a significant difference in IL?15 expression at 24, 48 and 72 hours between the 1.0?μmol/L acitretin+50 000?IU/ml IFN?αgroup and 5 000?IU/ml IFN?αgroup(all P<0.05). Conclusions Acitretin and IFN?αboth can inhibit the proliferation of and IL?15 expression in Hut78 cells, the inhibitory effects are enhanced with the increase in drug concentrations and treatment durations, and the combination of acitretin and IFN?α appears to have stronger inhibitory effects than acitretin or IFN?αalone.

Objective To observe the tolerance of contemporaneous multiplane operations in obstructive sleep apnea-hypopnea syndrome(OSAHS). Methods Twenty-three patients were enrolled. According to the different obstructive level, the different operations were chosen to complete upper airway reconstruction in contemporaneous operation group (group A, 13 patients). In the simple palatopharynx level obstructive group (group B, 10 patients), bilateral tonsillectomy and H-UPPP was chosen. Results The operation time in group A was significantly longer than that in group B:(121.0 ± 35.4) min vs.(80.7 ± 25.3) min, P<0.01. The hospital days and adverse events in two groups had no significantly difference (P>0.05). Conclusions Individual therapeutic schedule for OSAHS patients should be formulated. Operating the different obstructive levels simutaneously, which would solve upper airway occlusion and complete the upper airway reconstruction at the same time. No more adverse events happen, compared with the simple palatopharynx level obstructive group, though the operation time may be longer.

ObjectiveToinvestigatetheplasmidgenechangesinquinolone‐sensitivePseudomonasaeruginosa.Methods 31iso‐lates from January 2011 to December 2013 from various qualified clinical samples in the hospital were collected .In the 31 isolateds , 16 isolates proved sensitive to quinolones by using K‐B method were used as research objects in the study .The isolates growing ou‐side the sensitive ring of ciprofloxacin paper were selected to continuously transferred into other culture dishes until the resistance to quinolones were acquired .Plasmid transformation and extraction were performed on those isolates to confirm the existence of drug‐resistanceplasmidsacquired,andthroughPCRandgenesequenceanalysistodeterminethetypeofplasmids.Results 2iso‐lates of quinolone‐sensitive Pseudomonas aeruginosa acquired drug‐resistance plasmids qnrS and were resistant quinolones induced by continuous transferring for 9 times .Conclusion If antibiotics of inhibitory concentration were often used for the treatment of Pseudomonas aeruginosa infection ,drug‐resistance plasmids were acquired easily .

BACKGROUND: The aims of this study were to evaluate the abilities of direct sequencing (DS), peptide nucleic acid (PNA) clamping, and pyrosequencing methods to detect epidermal growth factor receptor (EGFR) mutations in formalin-fixed paraffin-embedded (FFPE) non-small cell lung carcinoma (NSCLC) samples and to correlate EGFR mutational status as determined by each method with the clinical response to EGFR tyrosine kinase inhibitors (TKIs). METHODS: Sixty-one NSCLC patients treated with EGFR TKIs were identified to investigate somatic mutations in the EGFR gene (exons 18-21). RESULTS: Mutations in the EGFR gene were detected in 38 of the 61 patients (62%) by DS, 35 (57%) by PNA clamping and 37 (61%) by pyrosequencing. A total of 44 mutations (72%) were found by at least one of the three methods, and the concordances among the results were relatively high (82-85%; kappa coefficient, 0.713 to 0.736). There were 15 discordant cases (25%) among the three different methods. CONCLUSIONS: All three EGFR mutation tests had good concordance rates (over 82%) for FFPE samples. These results suggest that if the DNA quality and enrichment of tumor cells are assured, then DS, PNA clamping, and pyrosequencing are appropriate methods for the detection of EGFR mutations.
Constriction ; DNA ; Genes, erbB-1 ; Humans ; Lung ; Lung Neoplasms ; Peptide Nucleic Acids ; Polymerase Chain Reaction ; Protein-Tyrosine Kinases ; Receptor, Epidermal Growth Factor ; Tyrosine