Objective To develop a system for retrieving information from clinical records of Traditional Chinese Medicine(TCM)based on generative large language models(LLMs).Methods Applicational needs of the system were analyzed,and entity types to be retrieved were identified.The functions,workflows,and architecture of the system were designed by combining the automatic retrieval capabilities of LLMs with human-in-the-loop(HITL).The software was developed using such frameworks as vLLM and Node.js.Interaction of multiple commercial/open source LLMs was implemented using OpenAI-compatible interfaces.The quality of information retrieved from LLMs was enhanced by prompt engineering.Results This system supported task allocation,automatic retrieval of structured information,and manual review.To evaluate its performance,the moonshot-v1-8k model was used to retrieve clinical records of TCM before manual edition was performed.Combining large language model pre-annotation with meticulous annotator edits improved accuracy by 26.6％compared to the BERT-BiLSTM-CRF model,and enhanced extraction efficiency by 1.6-fold relative to purely manual methods.Conclusion General generative LLMs can retrieve a wide range of entity information from TCM records with high accuracy and scalability.The design and implementation of this system approach may provide a useful reference for developing other biomedical information retrieval systems.

A case of secondary pulmonary infection in a patient with acute exacerbation of chronic obstructive pulmonary disease was admitted.The patient was initially giren piperacillin-tazobactam combined with levofloxacin for anti-infective treatment before the pathogen was identified.lafer,the HRZE anti-tuberculosis regimen was added,but the patient continued to experience high fever,yellow purulent sputum,and dyspnea.Subsequent,bronchoalveolar lavage fluid pathogen metagenomic sequencing revealed Nocardia terpenica.Clinical pharmacists,based on the patient's condition changes and in accordance with relevant guidelines and literature,suggested using various antimicrobial agents,including compound sulfamethoxazole tablets,linezolid glucose injection,amikacin injection combined with imipenem-cilastatin,to manage the patient's intolerance to sulfonamides and the bone marrow suppression induced by linezolid and to provide medication suggestions.The clinician adopted all the suggestions.Through the collaborative efforts of physicians and clinical pharmacists,the patient's condition improved,allowing for discharge with medication.Post-discharge,medication education and follow-up were conducted,leading to successful recovery.In this case,the clinical pharmacist reviewed domestic and international literature on Nocardia terpenica and the characteristics of antimicrobial drugs,and utilized their expertise to assist clinicians in treating rare infection cases,realizing their professional value.

Objective:To study the current situation and influencing factors of the ability of grassroots personnel involved in prevention and control of drinking water-borne endemic fluorosis based on a feasible ability theory.Methods:A self-developed evaluation tool was used to assess the ability of grassroots personnel involved in prevention and control of drinking water-borne endemic fluorosis. A questionnaire survey was conducted in 2024 via the Wenjuanxing platform to evaluate the current status of their ability and scores. A multivariate logistic regression model was employed to analyze the factors affecting the ability of these personnel.Results:A total of 2 879 questionnaires were collected, of which 2 751 were valid, with a validity rate of 95.55%. Among the valid questionnaires, 917 (33.33%) were filled out by personnel from the endemic areas. The overall ability score of grassroots personnel was (14.74 ± 2.56) points, with welfare scoring (3.45 ± 0.62) points, autonomy scoring (3.57 ± 0.89) points, subjectivity scoring (3.81 ± 0.69) points, and achievement scoring (3.79 ± 0.69) points. Multivariate logistic regression analysis showed that income and employment type were associated with the ability scores of grassroots personnel ( OR = 0.61, 1.31, 95% CI: 0.45 - 0.94, 0.89 - 1.94, P < 0.05). Conclusions:The ability of grassroots personnel involved in prevention and control of drinking water-borne endemic fluorosis is at a moderate level, and there is still room for improvement. Income and employment type are the main factors affecting their ability scores. Efforts should be made to improve salary and welfare benefits, refine professional title promotion mechanism, attract high-level talent, and enhance personnel stability, thus more effectively enhance the ability of grassroots personnel in prevention and control of drinking water-borne endemic fluorosis.

Objective:Based on the characteristics of grassroots disease prevention and control centers, medical institutions, and personnel involved in the prevention and control of drinking water-borne endemic fluorosis in China, the aim of this study is to develop a competency evaluation tool for grassroots personnel involved in the prevention and treatment of the disease.Methods:Through literature review and expert consultation, a competency evaluation tool was designed and developed. The tool was distributed nationwide via the Wenjuanxing platform. Valid data were collected and analyzed for item discrimination, validity, and reliability to assess its measurement effectiveness.Results:A total of 150 valid questionnaires were collected, covering 13 provinces across China (67 males, 83 females). The evaluation tool demonstrated significant differences in scores between high and low groups for all items ( P < 0.001). The correlation coefficients between each item and its corresponding factor ranged from 0.77 to 0.87, with factor loadings ranged from 0.68 to 0.97. In the validity analysis, root mean square error of approximation ( RMSEA) was 0.07, and standardized root mean square residual ( SRMR) was 0.03. The goodness-of-fit indices were as follows: goodness-of-fit index (GFI) = 0.94, normed fit index (NFI) = 0.96, incremental fit index (IFI) = 0.95, tucker-lewis index(TLI) = 0.96, and comparative fit index (CFI) = 0.96. In the reliability analysis, the overall Cronbach's α coefficient was 0.91, and the split-half coefficient was 0.88. Conclusions:The competency evaluation tool for grassroots personnel in the prevention and treatment of drinking water-borne endemic fluorosis exhibits high item discrimination, as well as good overall reliability and validity. It can be used to evaluate the competency of prevention and treatment personnel and to study influencing factors.

Objective:To study the current situation and influencing factors of the ability of grassroots personnel involved in prevention and control of drinking water-borne endemic fluorosis based on a feasible ability theory.Methods:A self-developed evaluation tool was used to assess the ability of grassroots personnel involved in prevention and control of drinking water-borne endemic fluorosis. A questionnaire survey was conducted in 2024 via the Wenjuanxing platform to evaluate the current status of their ability and scores. A multivariate logistic regression model was employed to analyze the factors affecting the ability of these personnel.Results:A total of 2 879 questionnaires were collected, of which 2 751 were valid, with a validity rate of 95.55%. Among the valid questionnaires, 917 (33.33%) were filled out by personnel from the endemic areas. The overall ability score of grassroots personnel was (14.74 ± 2.56) points, with welfare scoring (3.45 ± 0.62) points, autonomy scoring (3.57 ± 0.89) points, subjectivity scoring (3.81 ± 0.69) points, and achievement scoring (3.79 ± 0.69) points. Multivariate logistic regression analysis showed that income and employment type were associated with the ability scores of grassroots personnel ( OR = 0.61, 1.31, 95% CI: 0.45 - 0.94, 0.89 - 1.94, P < 0.05). Conclusions:The ability of grassroots personnel involved in prevention and control of drinking water-borne endemic fluorosis is at a moderate level, and there is still room for improvement. Income and employment type are the main factors affecting their ability scores. Efforts should be made to improve salary and welfare benefits, refine professional title promotion mechanism, attract high-level talent, and enhance personnel stability, thus more effectively enhance the ability of grassroots personnel in prevention and control of drinking water-borne endemic fluorosis.

A case of secondary pulmonary infection in a patient with acute exacerbation of chronic obstructive pulmonary disease was admitted.The patient was initially giren piperacillin-tazobactam combined with levofloxacin for anti-infective treatment before the pathogen was identified.lafer,the HRZE anti-tuberculosis regimen was added,but the patient continued to experience high fever,yellow purulent sputum,and dyspnea.Subsequent,bronchoalveolar lavage fluid pathogen metagenomic sequencing revealed Nocardia terpenica.Clinical pharmacists,based on the patient's condition changes and in accordance with relevant guidelines and literature,suggested using various antimicrobial agents,including compound sulfamethoxazole tablets,linezolid glucose injection,amikacin injection combined with imipenem-cilastatin,to manage the patient's intolerance to sulfonamides and the bone marrow suppression induced by linezolid and to provide medication suggestions.The clinician adopted all the suggestions.Through the collaborative efforts of physicians and clinical pharmacists,the patient's condition improved,allowing for discharge with medication.Post-discharge,medication education and follow-up were conducted,leading to successful recovery.In this case,the clinical pharmacist reviewed domestic and international literature on Nocardia terpenica and the characteristics of antimicrobial drugs,and utilized their expertise to assist clinicians in treating rare infection cases,realizing their professional value.

Objective:Based on the characteristics of grassroots disease prevention and control centers, medical institutions, and personnel involved in the prevention and control of drinking water-borne endemic fluorosis in China, the aim of this study is to develop a competency evaluation tool for grassroots personnel involved in the prevention and treatment of the disease.Methods:Through literature review and expert consultation, a competency evaluation tool was designed and developed. The tool was distributed nationwide via the Wenjuanxing platform. Valid data were collected and analyzed for item discrimination, validity, and reliability to assess its measurement effectiveness.Results:A total of 150 valid questionnaires were collected, covering 13 provinces across China (67 males, 83 females). The evaluation tool demonstrated significant differences in scores between high and low groups for all items ( P < 0.001). The correlation coefficients between each item and its corresponding factor ranged from 0.77 to 0.87, with factor loadings ranged from 0.68 to 0.97. In the validity analysis, root mean square error of approximation ( RMSEA) was 0.07, and standardized root mean square residual ( SRMR) was 0.03. The goodness-of-fit indices were as follows: goodness-of-fit index (GFI) = 0.94, normed fit index (NFI) = 0.96, incremental fit index (IFI) = 0.95, tucker-lewis index(TLI) = 0.96, and comparative fit index (CFI) = 0.96. In the reliability analysis, the overall Cronbach's α coefficient was 0.91, and the split-half coefficient was 0.88. Conclusions:The competency evaluation tool for grassroots personnel in the prevention and treatment of drinking water-borne endemic fluorosis exhibits high item discrimination, as well as good overall reliability and validity. It can be used to evaluate the competency of prevention and treatment personnel and to study influencing factors.

Circular RNA (circRNA) is a single-stranded circular closed RNA molecule formed from linear RNA through reverse splicing. circRNAs are stable, highly conserved, and tissue-specific. circRNAs can regulate physiological and pathological processes through various mechanisms such as formation of competing endogenous RNA and interaction with binding proteins. It has been recently revealed that circRNAs can be translated into peptides and proteins to participate in the initiation and development of cancer. circRNAs are promising diagnostic and prognostic markers for human cancers as well as potential drug targets for cancer therapy. This review summarized the research progresses related to circRNA-encoded peptides and proteins in a variety of cancers. These peptides and proteins are translated through two different mechanisms that depend on internal ribosome entry site and m6A, respectively. We also summarized the potential use of circRNA-encoded peptides and proteins in the diagnosis, treatment, prognosis and mechanistic studies of various cancers.
Humans ; Internal Ribosome Entry Sites ; Neoplasms/genetics* ; Peptides, Cyclic ; RNA/genetics* ; RNA, Circular/genetics*

Objective To study the influence of different support heights, support numbers and cross-sectional dimensions on support performance of NiTi thoracic aortic stents. Methods Twenty-seven scaffold models with different parameters were established by using AutoCAD 2016 and SoildWorks 2014 software. HyperMesh 14.0 was used for tetrahedral mesh division, and ABAQUS 2017 was used for support performance simulation analysis. Results With the decrease of support height, the support stiffness would increase; a larger cross-section size would lead to a larger support stiffness; with the increase of support numbers, the support stiffness would increase. Among the influencing factors of support performance, the order of influence degree was support height＞section size＞support numbers. Conclusions The research findings have certain guiding significance for the development and research of thoracic aortic stents, and provide theoretical basis for the selection and optimization of clinical stents.