The diagnosis of occupational radiation diseases, with the diagnostic criteria as main technical basis, has always been one of the emphases of China's occupational health work. This study compared the diagnostic standards for occupational radiation diseases between China and the International Labour Organization (ILO) across three aspects—types, classifications/grades, and diagnostic dose requirements—categorizing the diseases into tissue reactions and stochastic effects. ① For tissue reactions, 8 types are included both in China and ILO standards, 13 unique to China's standards, and 4 unique to the ILO standards; significant differences exist in classifaton/grading; China's standards are either consistent with or stricter (i.e., requiring higher diagnostic dose values) than the ILO standards. ② For stochastic effects (radiation-induced cancer), 12 types were included in both standards; China's standards provide more comprehensive coverage for certain malignancies (e.g., bone cancer); while 5 types were unique to the ILO standards have, some (e.g. salivary gland cancer) are primarily observed in non-occupational populations. Overall, China’s standards include more disease types and maintain more stringent diagnostic dose requirements. However, specific discrepancies require further deliberation during future revisions. Finally, based on these results, recommendations were provided for basic research and the evaluation of Chinese diagnostic criteria.

The diagnosis of occupational radiation diseases, with the diagnostic criteria as main technical basis, has always been one of the emphases of China's occupational health work. This study compared the diagnostic standards for occupational radiation diseases between China and the International Labour Organization (ILO) across three aspects—types, classifications/grades, and diagnostic dose requirements—categorizing the diseases into tissue reactions and stochastic effects. ① For tissue reactions, 8 types are included both in China and ILO standards, 13 unique to China's standards, and 4 unique to the ILO standards; significant differences exist in classifaton/grading; China's standards are either consistent with or stricter (i.e., requiring higher diagnostic dose values) than the ILO standards. ② For stochastic effects (radiation-induced cancer), 12 types were included in both standards; China's standards provide more comprehensive coverage for certain malignancies (e.g., bone cancer); while 5 types were unique to the ILO standards have, some (e.g. salivary gland cancer) are primarily observed in non-occupational populations. Overall, China’s standards include more disease types and maintain more stringent diagnostic dose requirements. However, specific discrepancies require further deliberation during future revisions. Finally, based on these results, recommendations were provided for basic research and the evaluation of Chinese diagnostic criteria.

Background: High-mobility group box protein 1 (HMGB1) is a chromatin-binding protein involved in arthritis, ischemia, sepsis, atherosclerosis, neurodegenerative disorders, meningitis, and cancer. HMGB1 exhibits dual roles in cancer, acting as either a tumor suppressor or oncoprotein depending on context. Objective: This research aimed to elucidate HMGB1’s functional significance in cutaneous squamous cell carcinoma (cSCC). Methods: We overexpressed HMGB1 in cSCC cell lines using recombinant adenovirus and examined its effects on cell proliferation, colony formation, and cell migration. Results: Immunohistochemical analysis revealed elevated HMGB1 expression levels in cSCC tissue relative to normal epidermis. To assess the influence of HMGB1, we employed recombinant adenoviruses expressing HMGB1 to transduce SCC cell lines (SCC12 and SCC13). Enhanced HMGB1 expression significantly promoted cellular proliferation and colony formation capacity.Notably, HMGB1 overexpression elevated the levels of proliferation regulators, including P63, SOX2, CDK4 and CDK6. Furthermore, HMGB1 overexpression substantially enhanced tumor invasiveness, accompanied by upregulation of epithelial-mesenchymal transition (EMT) biomarkers. Mechanistically, overexpression of HMGB1 enhanced transforming growth factor-β signaling by increasing phosphorylation of SMAD2/3, the key mediators of EMT. Conclusion These data imply that HMGB1 acts as a tumor-promoting factor in cSCC.

Objective This study reports on the obligatory parasitism of water mites Arrenurus sp. on Anopheles messeae at the Manzhouli Port, Inner Mongolia, China. Methods Duing July 2024, a survey on the mosquito diversity was conducted at the Manzhouli Port. Captured mosquitoes and their ectoparasites were identified to species level. Results A total of 1840 adult mosquitoes were collected, representing species from three genera: Culex(Cx. modestus, Cx. pipiens pallens), Aedes(Ae. dorsalis, Ae. flavidorsalis, Ae. flavescens), and Anopheles (An. messeae). Among all the mosqutioes specimens,3 out of 150 captured An. messeae were found to carry ectoparasitic mites, with number of 2,4,27 mites separately. Morphological and molecular identification reached the same result as water mites(Hydrachnidiae, Hydracrina). COI gene sequence showed 94% similarity with the closest species Arrenurus truncatellus. Conlusions Literature review suggests water mites are host-specific parasitism of mosquito species and herein with the first record of Arrenurus sp. parasiting on An. Messeae in the most high-latitude region globally.

Objective To investigate the effects of advanced maternal age(AMA)on synaptic pruning of the hippocampus of offspring rats.Methods Offspring of 3-month-old SD female rats and 3-month-old SD male rats were designated as the appropriate age group(Ctl group),while offspring of 12-month-old SD female rats and 3-month-old SD male rats were assigned to AMA group.Hippocampal tissues from both groups(n=3 per group at each time point)were subjected to Golgi staining on postnatal days(P)7,28,and 60.Dendritic complexity,spine density(DS),and morphology of immature brain neurons at different age stages were compared between the two groups.Results Compared with Ctl group,the dendritic length of AMA group on P7,P28,and P60 was significantly shortened,and the average number of intersections between dendrites and concentric circles on P28 and P60 was also significantly decreased(P<0.05).Compared with Ctl group,the dendritic spine density of AMA group was significantly decreased on P7,P28,and P60.Notably,the density of thin-shaped dendritic spines in AMA group was increased on P7,whereas the densities of stubby-shaped and mushroom-shaped dendritic spines were decreased(P<0.05).On P28 in AMA group,the densities of thin-shaped and mushroom-shaped dendritic spines were significantly increased compared with Ctl group,while the density of stubby-shaped dendritic spines was significantly decreased(P<0.05).The densities of stubby-shaped and mushroom-shaped dendritic spine were markedly lower in AMA group on P60 than those in Ctl group(P<0.05).Conclusions During postnatal brain development periods,there were abnormalities in synaptic pruning in the hippocampal region of the offspring of the AMA,which manifested as reduced dendritic complexity,decreased dendritic spine density,and disorganized dendritic spine morphology.It could be one of the key mechanisms underlying brain dysfunction in the offspring of AMA.

Objective To investigate the effects and underlying molecular mechanisms of typhaneoside(TYP)on lung adenocarcinoma based on network pharmacology and in vitro experiments.Methods TYP-lung adenocarcinoma-related genes were obtained from the prediction target website SwissTargetPrediction,The Cancer Genome Atlas(TCGA)database,GeneCards database,and Gene Expression Omnibus(GEO)database.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses were performed on the obtained TYP-lung adenocarcinoma-related genes.Protein-protein network interactions analysis was carried out using the String database,and molecular docking was conducted with the Vina software.These network pharmacological analysis methods were employed to explore the theoretical action pathways of TYP on lung adenocarcinoma.PC9 cells were divided into control group(normal culture),low-TYP group(treated with 50 μmol/L TYP for 48 h),high-TYP group(treated with 100 μmol/L TYP for 48 h),and ferrostatin-1(Fer-1)+TYP group(treated with 1 μmol/L Fer-1+100 μmol/L TYP for 48 h).The cell viability was detected by the CCK-8 assay,the cell proliferation ability was detected by the EdU assay,and the cell migration and invasion ability was detected by scratch assay,Transwell migration assay and Transwell invasion assay.The occurrence of ferroptosis was detected by reduced glutathione(GSH)assay kit,reactive oxygen species(ROS)assay kit,mitochondrial membrane potential assay kit,ferrous ion fluorescent probe,and transmission electron microscopy.Protein and mRNA expression levels of ferroptosis-related key molecules,solute carrier family 7 member 11(SLC7A11)and glutathione peroxidase 4(GPX4),were measured by Western blotting and reverse transcription-quantitative PCR(RT-qPCR).PC9 cells were transfected with SLC7A11 overexpression plasmid and divided into vector group(transfected with empty plasmid),vector+TYP group(transfected with empty plasmid and treated with 100 μmol/L TYP for 48 h),OE-SLC7A11 group(transfected with SLC7A11 overexpression plasmid)and OE-SLC7A11+TYP group(transfected with SLC7A11 overexpression plasmid and treated with 100 μmol/L TYP for 48 h).The molecular mechanism of SLC7A11 in the effect of TYP on PC9 was verified by CCK-8 assay,ferrous ion fluorescence probe,and protein expression levels of SLC7A11 and GPX4.Results A total of 73 TYP-lung adenocarcinoma-related target genes were predicted.GO analysis showed that the target genes were mainly involved in biological processes such as positive regulation of kinase activity,and were enriched in cellular components like transferase complex(transferring phosphorus-containing groups),revealing molecular functions such as transmembrane receptor protein tyrosine kinase activity.KEGG analysis identified 124 related pathways,mainly including epidermal growth factor receptor(EGFR)tyrosine kinase inhibitor resistance pathway.Protein-protein interaction network analysis obtained 7 core targets such as serine/threonine kinase 1(Akt1).Molecular docking results showed that the binding energies of TYP with core target genes and the ferroptosis-related proteins(SLC7A11 and GPX4)were all≤-5.0 kcal/mol,indicating significant binding activity.CCK-8 assay showed that TYP significantly inhibited PC9 cell viability(P<0.05).EdU assay demonstrated that the proportion of proliferating cells in TYP group was significantly lower than that in control group(P<0.05).Scratch assay,Transwell migration assay,and invasion assay revealed that,compared with control group,the migration and invasion abilities of PC9 cells in TYP group significantly decreased(P<0.05),and GSH content and mitochondrial membrane potential in TYP group also significantly decreased(P<0.05),while ROS and Fe2+contents increased significantly(P<0.01),and protein and mRNA expression levels of SLC7A11 and GPX4 decreased significantly(P<0.05).Transmission electron microscopy results showed that cells in TYP group exhibited specific ferroptosis-related changes such as reduced mitochondrial cristae and increased membrane density,compared with control group.Compared with vector+TYP group,OE-SLC7A11+TYP group had higher cell viability(P<0.05),lower Fe2+content(P<0.05),and higher protein expression levels of SLC7A11 and GPX4(P<0.05).Conclusion TYP may induce ferroptosis in lung adenocarcinoma cells and inhibit their malignant behaviors such as proliferation,migration and invasion by regulating the SLC7A11-GPX4 axis.

Knee osteoarthritis is a common joint disease within osteoarthritis,characterized by pain,swelling,and limited functionality as the main clinical manifestations.In severe cases,it affects daily life and falls under the category of"impediment syndrome"or"bone impediment"in TCM.The author believes that the theory of"bones,tendons,and muscles"is closely related to this disease.Treatment should focus on simultaneously nourishing the liver,spleen and kidneys,considering tendons,bones and muscles,while also dispelling wind,cold and dampness.The clinical application of Sanbi Decoction has shown good efficacy,and this discussion aimed to provide ideas for the diagnosis and treatment of knee osteoarthritis.

AIM To explore the clinical effects of Supplemented Baihe Gujin Decoction on elderly patients with postoperative pulmonary infection following non-small cell lung cancer surgery.METHODS Ninety-two patients were randomly assigned into control group(46 cases)for 1-week intervention of conventional treatment,and observation group(46 cases)for 1-week intervention of both Supplemented Baihe Gujin Decoction and conventional treatment.The changes in clinical effects,TCM syndrome scores,immune function indices(CD3+,CD4+,CD8+,CD4+/CD8+),inflammatory indices(CRP,PCT,TNF-α),serum indices(sTREM-1,CD40L,NLR)and incidence of adverse reactions were detected.RESULTS The observation group demonstrated higher total effective rate than the control group(P＜0.05).After the treatment,the two groups displayed decreased TCM syndrome scores,CD8+,inflammatory indices,serum indices(P＜0.05),and increased CD3+,CD4+,CD4+/CD8+(P＜0.05),especially for the observation group(except for CD4+,CD8+)(P＜0.05).CONCLUSION For the elderly patients with postoperative pulmonary infection following non-small cell lung cancer surgery,Supplemented Baihe Gujin Decoction can safely and effectively relieve clinical symptoms,enhance immune functions,reduce serum sTREM-1,CD40L levels and NLR,and control inflammatory responses.

Objective:To explore the effect of empowerment-authorized health management on the rehabilitation,self-efficacy and quality of life in patients with non-ST-segment elevation acute coronary syndrome(NSTE-ACS)after per-cutaneous coronary intervention(PCI).Methods:This randomized controlled study enrolled 100 NSTE-ACS patients ad-mitted in the First Affiliated Hospital of Xinjiang Medical University between January 2021 and June 2023.Patients were randomly divided into control group(n=50,routine nursing)and intervention group(n=50,empowerment-authorized health management).Both groups were intervened for 6 months.The scores of heart health self-efficacy and self-man-agement(HH-SESM)scale,China questionnaire of quality of life in patients with cardiovascular diseases(CQQC),cardi-ac function indexes,serum levels of N terminal pro brain natriuretic peptide(NT-proBNP)and cardiac troponin I(cTnI),6min walking distance(6MWD),satisfaction and incidence of adverse events were compared between the two groups.Re-sults:Compared to patients in the control group after nursing,those in the intervention group had significant higher HH-SESM total score[(79.34±6.18)points vs.(62.33±6.17)points],CQQC total score[(116.24±7.13)points vs.(83.27±7.11)points],left ventricular ejection fraction(LVEF)[(59.32±4.36)％vs.(51.22±4.63)％],6min walk-ing distance(6MWD)[(363.61±12.51)m vs.(299.13±13.54)m]and satisfaction(86.96％vs.68.09％)(P＜0.05 or＜0.01),and significant lower left ventricular end-systolic diameter(LVESd)[(32.44±3.91)mm vs.(39.25±3.61)mm],left ventricular end-diastolic diameter(LVEDd)[(41.23±4.18)mm vs.(46.17±5.41)mm],NT-proBNP[(6.16±1.58)pg/ml vs.(12.51±3.27)pg/ml]and cTnI[(2.65±0.62)ng/ml vs.(5.60±1.81)ng/ml](P＜0.001 all).The incidence of adverse events in the intervention group(4.35％)was significantly lower than that of control group(19.15％,P=0.027).Conclusion:Empowerment-authorized health management mode was beneficial to the recovery of cardiac function and significantly improved self-efficacy and quality of life in patients with non-ST-segment elevation a-cute coronary syndrome.

Caenorhabditis elegans,as an emerging model organism,has been widely used in multiple disciplines such as medicine,life science,and environmental science in recent years,due to its charac-teristics of short life cycle,clear genetic background,highly conserved evolution,complete genome analysis and excellent fitting between experimental data and human results.It also shows unique advan-tages in the field of toxicology.This paper summarizes its advantages in toxicological research starting from the biological characteristics of C.elegans,introduces the toxicological research methods and progress based on the C.elegans model,focuses on demonstrating its applications in environmental fo-rensic medicine and forensic toxicology,and looks forward to the application of the relevant results in the field of forensic science.