OBJECTIVE To explore the change of adaptive cost of Klebsiella pneumoniae in subinhibitory concen-tration of Epigallocatechin gallate(EGCG)for subculture of 14 days so as to provide experimental support for clinical transformation of EGCG.METHODS The drug resistance genes in the K.pneumoniae strains were ana-lyzed by whole genome sequencing technology.The change of biofilm formation ability was detected by crystal vio-let staining,the transcriptional levels of quorum sensing gene luxS and its regulatory genes(lsrK and lsrR)as well as virulence genes(aerobactin and rmpA)were detected by means of fluorescent quantitative polymerase chain reaction(PCR).The pathogenicity of the K.pneumoniae was tested through survival assay of greater wax moth.RESULTS The whole genome sequencing showed that 1 strain of non-mucous CRKP and 2 strains of mucous CRKP were ST11,all of which carried bla KPC-2 gene.There was no change in the growth curve of sub-culture after treatment with subinhibitory concentration of EGCG;the biofilm forming ability was reduced.The expression level of luxS gene was higher in the non-treatment group.The transcriptional level of ionophore aer-obactin was inhibited with the increase of subculture days and subinhibitory concentration;the transcription ability of the virulence gene mucous phenotype regulatory gene rmpA was inhibited as well.The survival rate of greater wax moth increased,and there was significant difference(P＜0.05).CONCLUSIONS The growth rate of K.pneumoniae strains dose not change after the treatment with the subinhibitory concentration of EGCG.The bio-film formation and the transcriptional levels of virulence genes and quorum sensing genes as well as the pathoge-nicity are inhibited,which may provide experimental bases for clinical application of EGCG.

OBJECTIVE To explore the change of adaptive cost of Klebsiella pneumoniae in subinhibitory concen-tration of Epigallocatechin gallate(EGCG)for subculture of 14 days so as to provide experimental support for clinical transformation of EGCG.METHODS The drug resistance genes in the K.pneumoniae strains were ana-lyzed by whole genome sequencing technology.The change of biofilm formation ability was detected by crystal vio-let staining,the transcriptional levels of quorum sensing gene luxS and its regulatory genes(lsrK and lsrR)as well as virulence genes(aerobactin and rmpA)were detected by means of fluorescent quantitative polymerase chain reaction(PCR).The pathogenicity of the K.pneumoniae was tested through survival assay of greater wax moth.RESULTS The whole genome sequencing showed that 1 strain of non-mucous CRKP and 2 strains of mucous CRKP were ST11,all of which carried bla KPC-2 gene.There was no change in the growth curve of sub-culture after treatment with subinhibitory concentration of EGCG;the biofilm forming ability was reduced.The expression level of luxS gene was higher in the non-treatment group.The transcriptional level of ionophore aer-obactin was inhibited with the increase of subculture days and subinhibitory concentration;the transcription ability of the virulence gene mucous phenotype regulatory gene rmpA was inhibited as well.The survival rate of greater wax moth increased,and there was significant difference(P＜0.05).CONCLUSIONS The growth rate of K.pneumoniae strains dose not change after the treatment with the subinhibitory concentration of EGCG.The bio-film formation and the transcriptional levels of virulence genes and quorum sensing genes as well as the pathoge-nicity are inhibited,which may provide experimental bases for clinical application of EGCG.

OBJECTIVE: To identify potential mutation in a child clinically diagnosed as Noonan syndrome and to provide genetic counseling and prenatal diagnosis for his family. METHODS: Genomic DNA was extracted from peripheral blood samples of the patient and his parents, and amniotic fluid was taken from the mother during the second trimester. Next generation sequencing (NGS) was used to screen potential mutations from genomic DNA. Suspected mutation was verified by Sanger sequencing. RESULTS: A heterozygous c.4A>G (p.Ser2Gly) mutation of the SHOC2 gene was identified in the patient but not among other family members including the fetus. CONCLUSION The Noonan syndrome is probably caused by the c.4A>G mutation of the SHOC2 gene. NGS is helpful for the diagnosis of complicated genetic diseases. SHOC2 gene mutation screening is recommended for patient suspected for Noonan syndrome.
Child ; Female ; Genetic Testing ; High-Throughput Nucleotide Sequencing ; Humans ; Intracellular Signaling Peptides and Proteins ; Mutation ; Noonan Syndrome ; Pregnancy ; Prenatal Diagnosis

Objective To explore the microbial infection and the related factors in patients with periodontal-endodontic combined lesions(PECL),in order to provide reference for the control and prevention of clinical infec-tion.Methods 36 patients with PECL were selected as observation group;36 cases(36 teeth)were selected as con-trol group.Samples of periodontal pockets and root canal samples were collected to detect the infection of microorgan-ism,and the risk factor of the occurrence of PECL was analyzed.Results 23 teeth in the observation group were detected microorganisms,the detection rate was 63.9%,such as Neisseria,Corynebacterium,actinomyces,Campy-lobacter,Fusobacterium were the main microorganisms,including 23 teeth for periodontal pockets were detected in the microorganism,the detection rate was 100.0%,and 14 cases of root canal detection of microbial,the detection rate was 60.9%;7 teeth in the control group was found in microbes,and the detection rate was 19.4%,the detection rate in the observation group was significantly higher than that of control group(χ2 =14.63,P <0.05).The tooth week source PECL dependent variable y multi factors logistic regression analysis showed that Neisseria,Corynebacterium, actinomyces,Fusobacterium infection were periodontal source PECL risk factors(OR =1.462,1.379,1.467,1.665, all P <0.05).Conclusion The occurrence of periodontal source PECL is closely related to the microbial infection, and the clinical treatment can improve the cure rate.