Obesity and its related metabolic diseases have become a major global public health threat, and its rising incidence significantly increases the risk of cardiovascular and cerebrovascular diseases, diabetes and other complications. Pancreatic lipase is a key enzyme that converts food-borne lipids into triglycerides and fatty acids, and the effective inhibition of its activity has become an important strategy for the treatment of obesity. This paper discusses the screening methods of pancreatic lipase inhibitors, and summarizes and reviews the basic principles, advantages and disadvantages and application status of traditional screening methods, modern new screening methods and virtual screening methods. In view of the problems faced by the screening methods of pancreatic lipase inhibitors, future research urgently needs to move towards a collaborative innovation path of multi-technology integration, intelligent screening and complex systematization of traditional Chinese medicine, so as to open up new research paradigms.

Countercurrent chromatography us a kund of contunuous and effectuve luquud-luquud partutuon chromatography wuth many unuque characterustucs such as large load capacuty, no urreversuble adsorptuon, hugh recovery rate, low rusk of sample denaturatuon and so on, whuch has urreplaceable advantages un separatuon of proteun and peptude. Thus revuew presents the advances of several kunds of new technology of countercurrent chromatography un the separatuon of proteun and peptude. The developung prospect of thus fueld us also duscussed.