ObjectiveTo investigate the mechanism by which activation of α7nicotinic acetylcholine receptors （α7nAChR） optimizes brown adipose tissue （BAT） thermogenesis in obese mice. MethodsObesity was induced in C57BL/6J mice via a high-fat diet. Fifty 8-week-old mice were randomly assigned to five groups： low-fat diet （Control）， high-fat diet （HFD）， high-fat diet plus a β3-adrenergic receptor agonist （HFD+β3）， high-fat diet plus a β3-adrenergic receptor agonist and an α7nAChR selective agonist （HFD+β3+GTS-21）， and high-fat diet plus a β3-adrenergic receptor agonist and an α7nAChR selective antagonist （HFD+β3+α-BGT）. Haematoxylin and eosin （HE） staining was used to evaluate BAT morphology. Transmission electron microscopy was performed to assess mitochondrial number and lipid droplet morphology in adipocytes. ELISA was used to measure levels of tumour necrosis factor-α （TNF-α）， interleukin（IL）-1β，IL-10， transforming growth factor-β （TGF-β）， cyclic adenosine monophosphate （cAMP）， and norepinephrine （NE）. RT-qPCR was conducted to determine mRNA expression levels of vascular endothelial growth factor A （VEGF-A）， nitric oxide synthase 2 （NOS2）， arginase 1 （Arg1）， uncoupling protein 1 （UCP1）， PR domain-containing protein 16 （PRDM16）， and peroxisome proliferator-activated receptor γ coactivator-1α （PGC-1α） in BAT. Immunohistochemistry was used to detect macrophage markers （CD31， CD86， and CD206）. Protein expression levels of UCP1， α7nAChR， nuclear factor-κB p65（NF-κB p65）， phosphorylated Janus kinase 2 （p-JAK2）， and phosphorylated signal transducer and activator of transcription 3 （p-STAT3） were analyzed by Western blot. ResultsCompared with the Control group， the HFD group showed enlarged lipid droplets and reduced mitochondrial numbers in adipocytes， accompanied by increased TNF-α and IL-1β and decreased IL-10， TGF-β， Arg1， and VEGF-A （all P<0.01）. Compared with the HFD+β3 group， the HFD+β3+GTS-21 group showed a smaller lipid droplet area and a higher mitochondrial number， along with reduced levels of pro-inflammatory factors， NOS2， and NF-κB p65， and increased levels of anti-inflammatory factors， Arg1， and phosphorylation of JAK2 and STAT3 （all P<0.01）. ConclusionActivation of α7nAChR in combination with a β3-adrenergic receptor agonist effectively enhances thermogenesis， alleviates local adipose tissue hypoxia， and shifts the local inflammatory phenotype toward an M2-like state， thereby reducing inflammation in brown adipose tissue and improving its thermogenic capacity.

Aim To investigate the role and mechanism of RNA binding protein zinc finger protein 36(ZFP36)in vascular calcification.Methods Human aortic smooth muscle cells were infected with ZFP36-overex-pressing virus or transfected with ZFP36 siRNA,followed by high phosphate stimulation to observe the effect of overex-pression or knockdown of ZFP36 on smooth muscle cell calcification;RNA immunoprecipitation and stability experiments were used to detect whether Runx2 was the target gene of ZFP36;Smooth muscle specific ZFP36 knockout mice were generated and vitamin D was used to induce vascular calcification.Alizarin Red and Von Kossa staining were used to observe calcification of aortic vessels,and Western blot was used to detect Runx2 protein expression.Results ZFP36 expression was elevated under calcification-stimulating conditions.Overexpression of ZFP36 alleviated high phos-phate-induced smooth muscle cell calcification,while knockdown of ZFP36 exacerbated calcification.ZFP36 could bind to Runx2 mRNA and promote its degradation.At the cellular level,ZFP36 could inhibit smooth muscle cell calcification via Runx2.At the animal level,knockout of ZFP36 in smooth muscle exacerbated vascular calcification induced by vita-min D.Conclusion ZFP36 inhibits vascular calcification by targeting Runx2 mRNA and suppressing its expression in smooth muscle cells.

Objective To analyze the characteristics of serological prenatal screening results of pregnant women in Xinjiang,and pro-vide reference basis for the prevention and control of birth defects in Xinjiang.Methods The clinical data of 50781 pregnant women with singleton pregnancies who underwent mid-trimester serological prenatal screening at the Prenatal Diagnosis Center,Urumqi Maternal and Child Health Care Hospital from January 2022 to December 2023 were retrospectively analyzed.The levels of alpha-fetoprotein(AFP),free beta-chorionic gonadotropin(Freeβ-HCG)and free ultra Estriol(uE3)in the serum of pregnant women corresponding to different weight groups and pre-delivery age groups,and the differences in AFP,Freeβ-HCG and uE3 levels among 12 prefectures and 7 ethnic groups with the largest group of pregnant women were compared.Results The high-risk rate of serological prenatal screening in Xin-jiang was 6.00％,the prenatal diagnosis rate was 27.56％,and the positive predictive value was 1.84％.The levels of AFP,Freeβ-HCG and uE3 were inversely correlated with maternal weight and expected age before delivery.There were differences in the levels of AFP,Freeβ-HCG,uE3 in the serum of pregnant women from various prefectures and ethnic groups in Xinjiang(P＜0.05).Conclusion This study preliminarily determined the median of serological prenatal screening for AFP,Freeβ-HCG and uE3 in 12 prefectures and 7 permanent ethnic groups in Xinjiang.The prenatal screening center has revised the median system for prefectures and ethnic groups,which is of great significance for improving the service capacity of eugenics and childcare in Xinjiang.

Aim To investigate the role and mechanism of RNA binding protein zinc finger protein 36(ZFP36)in vascular calcification.Methods Human aortic smooth muscle cells were infected with ZFP36-overex-pressing virus or transfected with ZFP36 siRNA,followed by high phosphate stimulation to observe the effect of overex-pression or knockdown of ZFP36 on smooth muscle cell calcification;RNA immunoprecipitation and stability experiments were used to detect whether Runx2 was the target gene of ZFP36;Smooth muscle specific ZFP36 knockout mice were generated and vitamin D was used to induce vascular calcification.Alizarin Red and Von Kossa staining were used to observe calcification of aortic vessels,and Western blot was used to detect Runx2 protein expression.Results ZFP36 expression was elevated under calcification-stimulating conditions.Overexpression of ZFP36 alleviated high phos-phate-induced smooth muscle cell calcification,while knockdown of ZFP36 exacerbated calcification.ZFP36 could bind to Runx2 mRNA and promote its degradation.At the cellular level,ZFP36 could inhibit smooth muscle cell calcification via Runx2.At the animal level,knockout of ZFP36 in smooth muscle exacerbated vascular calcification induced by vita-min D.Conclusion ZFP36 inhibits vascular calcification by targeting Runx2 mRNA and suppressing its expression in smooth muscle cells.

Objective To analyze the characteristics of serological prenatal screening results of pregnant women in Xinjiang,and pro-vide reference basis for the prevention and control of birth defects in Xinjiang.Methods The clinical data of 50781 pregnant women with singleton pregnancies who underwent mid-trimester serological prenatal screening at the Prenatal Diagnosis Center,Urumqi Maternal and Child Health Care Hospital from January 2022 to December 2023 were retrospectively analyzed.The levels of alpha-fetoprotein(AFP),free beta-chorionic gonadotropin(Freeβ-HCG)and free ultra Estriol(uE3)in the serum of pregnant women corresponding to different weight groups and pre-delivery age groups,and the differences in AFP,Freeβ-HCG and uE3 levels among 12 prefectures and 7 ethnic groups with the largest group of pregnant women were compared.Results The high-risk rate of serological prenatal screening in Xin-jiang was 6.00％,the prenatal diagnosis rate was 27.56％,and the positive predictive value was 1.84％.The levels of AFP,Freeβ-HCG and uE3 were inversely correlated with maternal weight and expected age before delivery.There were differences in the levels of AFP,Freeβ-HCG,uE3 in the serum of pregnant women from various prefectures and ethnic groups in Xinjiang(P＜0.05).Conclusion This study preliminarily determined the median of serological prenatal screening for AFP,Freeβ-HCG and uE3 in 12 prefectures and 7 permanent ethnic groups in Xinjiang.The prenatal screening center has revised the median system for prefectures and ethnic groups,which is of great significance for improving the service capacity of eugenics and childcare in Xinjiang.

Radiotherapy is one of the main treatment methods for malignant tumors, which can cause the radiation damage to normal tissues. Radiation-induced skin injury (RISI) is one of the main adverse reactions caused by radiotherapy. The main clinical manifestations of RISI are dermatitis, ulcer, erosion and necrosis, which seriously affect the quality of life and treatment effect of tumor radiotherapy patients, and even affect the overall survival of patients. The pathological mechanism of RISI is still unclear. Some studies have shown that inflammation and oxidative stress are the main causes of RISI. RISI can be divided into acute and chronic RISI according to the different onset time, and different treatment strategies can be formulated according to the severity of the injury. In this article, clinical manifestations, classification, pathogenesis, prevention and treatment of RISI are comprehensively summarized.

Objective To discuss the application value of video health education mode in patient's self-maintenance of totally implantable venous access port(TIVAP).Methods A total of 208 patients,who received implantation of TIVAP at the Second Affiliated Hospital of Naval Military Medical University of China From September 2021 to December 2022,were enrolled in this study.The patients were divided into control group(n=108)and observation group(n=100).Traditional health education was conducted for the patients of the control group,while on the basis of traditional health education an additional video health education based on visual communication theory was carried out for the patients of the observation group.Evaluation of self-care ability(ESCA)was used to evaluate the self-care ability before the intervention and 6 months after the implantation of TIVAP in the two groups.The complications,the incidence of adverse events,and the indwelling duration of the infusion port were compared between the two groups.Results Before intervention,the differences in each dimension score and total score of ESCA table between the two groups were not statistically significant(all P＞0.05).After intervention,the each dimension score and total score of ESCA table in the observation group were significantly higher than those in the control group(all P＜0.05).After intervention,both the total incidence of complications and the total incidence of adverse events in the observation group were remarkably lower than those in the control group(both P＜0.05),besides,the indwelling duration of the infusion port in the observation group was obviously longer than that in the control group.Conclusion The use of video health education mode can significantly improve the self-care ability of patients carrying a TIVAP,reduce the incidence of complications and adverse events,and extend the effective duration of TIVAP.Therefore,it is an effective health education mode.

Lung cancer is the leading cause of cancer-related deaths， and standard treatments for lung cancer， including surgery， radiotherapy， chemotherapy， targeted therapy， and immunotherapy， have shown significant clinical effects. However， current available treatment strategies are still unable to cure the disease. Since the majority of lung cancer patients are diagnosed at an advanced stage， surgical options are often lost， and the primary approach is typically a combination of radiotherapy and chemotherapy. However， the adverse reactions associated with these treatments limit their effectiveness and application， and the damage caused to normal tissues is often more severe than that inflicted on the tumor. Currently， traditional Chinese medicine （TCM） has been used as part of combination therapy for cancer treatment due to its unique system of syndrome differentiation， flexible compatibility， and safety and efficacy. TCM prescriptions and single drugs with multiple components and targets can simultaneously regulate multiple pathways. As reported， among numerous pathways involved in the regulation of lung cancer， the nuclear factor-κB （NF-κB） signaling pathway plays a key role in inducing cell transcription and is one of the main pathways involved in the occurrence and development of lung cancer. It can specifically regulate inflammatory responses， cell proliferation， apoptosis， invasion and metastasis， angiogenesis， and multidrug resistance in lung cancer. TCM prescriptions and single drugs can inhibit lung cancer cell proliferation， invasion， and metastasis， induce apoptosis and autophagy in lung cancer cells， suppress angiogenesis， regulate immune function， and treat multidrug resistance by regulating the NF-κB signaling pathway. Therefore， they play a role in intervening in lung cancer. However， there is currently a lack of systematic literature research that comprehensively summarizes and elucidates these aspects in China and abroad. Therefore， it is important to provide a systematic elucidation of the mechanism underlying the regulation of the NF-κB signaling pathway in lung cancer and review TCM interventions in lung cancer based on the NF-κB signaling pathway. This study is expected to provide references for the clinical application of lung cancer therapeutic drugs and the development of new drugs.

Objective:To observe the efficacy of 3D printing-assisted hematoma puncture and drainage in the treatment of hypertensive intracerebral hemorrhage and to explore the factors affecting postoperative brain dysfunction.Methods:A retrospective Case-control study was conducted to select 168 hypertensive intracerebral hemorrhage patients who were treated with 3D printing assisted hematoma puncture and drainage in the People′s Hospital of Yuechi County from January 2020 to September 2022 as the observation group, and 125 hypertensive intracerebral hemorrhage patients who were treated with CT guided hematoma puncture and drainage in the People′s Hospital of Yuechi County at the same time as the control group. The clinical efficacy of the two groups of patients was compared. According to the occurrence of postoperative brain dysfunction, the patients in the observation group were divided into normal brain function group ( n=121) and brain dysfunction group ( n=47). The clinical data of age, preoperative cerebral hernia, blood loss, ventilator-assisted ventilation, postoperative Glasgow coma index score (GCS) and postoperative complications were compared between the two groups. Multivariate Logistic regression was used to analyze the factors affecting postoperative brain dysfunction in the observation group, and a line chart model was constructed and its predictive efficiency was evaluated. The measurement data of normal distribution is expressed as mean ± standard deviation ( ± s), and independent sample t-test is used for inter group comparison. Chi-square test was used for comparison between count data groups. Results:The proportion of the drainage tube in the hematoma, hematoma clearance rate at 3 and 7 days after surgery, total effective rate of treatment, and GCS score at 1 week after surgery in the observation group were 88.69%(149/168), 54.17%(91/168), 96.43%(162/168), 92.86%(156/168), and 10.72±3.45, respectively, the control group was 75.20%(94/125), 36.80%(46/125), 81.60%(102/125), 76.80%(96/125), and 9.08±3.22, respectively, the difference between the two groups was statistically significant ( P<0.05). Advanced age ( OR=1.983, 95% CI: 1.169-2.732, P=0.017), preoperative cerebral hernia ( OR=1.532, 95% CI: 1.113-2.139, P=0.029), bleeding volume ≥ 50 mL ( OR=2.538, 95% CI: 1.802-3.347, P=0.003), postoperative GCS score 3-5 ( OR=2.874, 95% CI: 2.265-3.449, P<0.001), postoperative hypoxemia ( OR=2.251, 95% CI: 1.673-2.842, P=0.010) and postoperative chronic hydrocephalus ( OR=1.642, 95% CI: 1.214-2.021, P=0.022) were risk factors for postoperative brain dysfunction, while ventilator-assisted ventilation ( OR=0.656, 95% CI: 0.132-0.828, P=0.038) was protective factors. The internal verification of the line chart model by Bootstrap resampling method shows that the model has high differentiation, accuracy and validity. Conclusion:The application of 3D printing-assisted localization in hematoma puncture and drainage can improve the puncture condition and the hematoma clearance rate and clinical effect of patients with hypertensive intracerebral hemorrhage. Advanced age, preoperative cerebral hernia and bleeding volume are related to postoperative brain dysfunction. Clinical attention should be paid to patients with risk indicators of postoperative brain dysfunction.

A split-plot experiment was conducted to study the optimum planting density of Nuomi yam in directional groove shallow cultivation. Nuomi yam was the main local cultivar of Dioscorea. The study of this paper may help to give valuable instructions of local production. Three different planting densities and shallow groove diameter were designed in the experiment. The results showed that the fresh weight of aerial part per plant, yield and planting benefit of Nuomi yam tuber were the highest when the planting density was 12 000 plants/hm~2 and the diameter of shallow growth groove was 11.0 cm, while they were the lowest when the planting density was 22 500 plants/hm~2 and the diameter of shallow growth groove was 7.5 cm. Along with the increase of planting density, the fresh weight of aerial part, tuber, fresh yield, benefit and commercial tuber rate of Nuomi yam also decreased. The commercial tuber rate of Nuomi yam of 11.0 cm diameter in shallow growing pot was the highest, while it was the lowest with no shallow growing pot. On the contrary, the content of amylopectin and mucin was the highest when there was no shallow groove, and was the lowest when the diameter of shallow groove was 7.5 cm, although there was no significant difference between the two treatments. Therefore, Nuomi yam with the directional cultivation combination of planting density of 12 000 plants/hm~2 and shallow groove diameter of 11.0 cm showed high yield, good appearance quality, high planting benefit and no obvious adverse effect on internal quality.
Dioscorea