Objective: To investigate the hepatitis C prevention and control knowledge among clinicians in Jiaxing City, Zhejiang Province, so as to provide the evidence for intensified training and improved diagnosis and treatment of hepatitis C among clinicians. Methods: In November, 2021, clinicians were sampled using a stratified random sampling method from a city-level and a county (district)-level hepatitis C designated hospital in Jiaxing City. A questionnaire survey was performed using the Questionnaire for Hepatitis C Prevention and Control Knowledge among Clinicians, and the awareness of basic knowledge, professional knowledge and related knowledge about hepatitis C prevention and control among clinicians were descriptively analyzed. Results: A total of 186 questionnaires were allocated and 179 valid questionnaires were recovered, with an effective recovery rate was 96.24%. The respondents included 107 men (59.78%) and 72 women (40.22%) and had a mean age of (37.06±9.46) years. There were 107 respondents with a bachelor degree (59.78%), 56 with junior professional titles (31.28%), and 170 from non-infectious disease departments (94.97%). The awareness of basic hepatitis C prevention and control knowledge was 96.09%, and the awareness of “Transfusion of blood containing hepatitis C virus may acquire hepatitis C” was high (98.88%), and the awareness of “Hepatitis C can be cured” was low (77.09%). The awareness of professional hepatitis C prevention and control knowledge was 3.91% to 100.00%, and the awareness of “Pathogens of hepatitis C” (100.00%) and “Recommended screening populations for hepatitis C” (86.59%) was high, while the awareness of “There are two categories of hepatitis C cases: clinically diagnosed cases and confirmed cases” (3.91%) and “Clinical diagnosis of hepatitis C: positive anti-HCV antibody + any one of abnormal liver function or epidemiological history or clinical symptoms” (3.91%) was low. The awareness rates of “The state has included antiviral agents against hepatitis C into medical insurance” was and “Antiviral agents against hepatitis C are reimbursed in outpatient and inpatient departments of our hospital” were 81.56% and 59.78%, respectively. There were 69 clinicians participating hepatitis C-related training within one year (38.55%), and the awareness of clinicians that had participated in hepatitis C-related training had a higher awareness rate of basic hepatitis C prevention and control knowledge than those without participation (100.00% vs. 93.64%, P<0.05). Conclusion The awareness of basic hepatitis C prevention and control knowledge is high among clinicians in Jiaxing City; however, the training on diagnosis and classification criteria of hepatitis C and related medical insurance policy require to be improved.

Long non-coding RNA (lncRNA) is defined as non-protein coding transcript longer than 200 nucleotides. In the form of RNA, it affects gene expression at the epigenetic, transcriptional and post-transcriptional levels, and is widely involved in the body's pathophysiological processes. This review summarizes the research progress of lncRNA in the field of parasitology in order to find new targets for the prevention and treatment of parasitic diseases.

A 64-year-old male patient with type 2 diabetes mellitus received metformin 0.5 g thrice daily, acarbose 100 mg thrice daily, and saxagliptin 5 mg once daily orally. The patient developed itching and erythema symptoms during the treatments. The patient learned that metformin could cause adverse reactions such as pruritus and rash by reading the drug label. Then the metformin was stopped by himself and his skin symptoms were relieved within a few days. Due to elevated blood glucose, the patient took metformin again and developed erythema and papules 2 days later, but he didn′t stop the drug. After that, swelling of both lower limbs, accompanied by skin erosion and exudation appeared gradually. Laboratory tests showed that eosinophil count was 0.66×10 9/L. Drug eruptions caused by metformin was considered. All oral hypoglycemic drugs were stopped and symptomatic treatments such as antihistamine and glucocorticoid were given. Five days later, his erythema and papules gradually subsided, and erosive surface of both lower limbs crusted without exudation. Laboratory tests showed eosinophil count 0.06×10 9/L. Dermatitis did not recur after the patient switched to insulin to control blood glucose.

Minocycline is a semi-synthetic tetracycline antibiotic. In recent years, minocycline has been used in the treatment of chronic infections caused by multi-drug resistant bacteria and chronic diseases such as pemphigus and rheumatoid arthritis due to its lower incidence of bacterial resistance and good antibacterial effect. Long term use of oral minocycline can cause skin hyperpigmentation. According to the location, clinical features, and histopathological changes, minocycline-induced skin hyperpigmentation (MISH) is divided into 4 subtypes, but the definition of subtype 4 remains controversial. The incidence of MISH varies greatly in different studies, which may be closely related to factors such as patients′ underlying pathophysiological status, comorbid diseases, dose, and duration of treatment. For those patients who need to take minocycline for a long time, skin color should be observed. Once skin color changes were found, relevant examinations (e.g. skin biopsy) should be carried out in time to make a definite diagnosis as soon as possible. Patients with MISH should pay attention to sun protection. Reducing the dose or stopping minocycline is an effective strategy to improve MISH. Q-switched laser or picosecond laser is recommended to treat pigmentation which can not be recovered after drug withdrawal.

A 64-year-old male patient with type 2 diabetes mellitus received metformin 0.5 g thrice daily, acarbose 100 mg thrice daily, and saxagliptin 5 mg once daily orally. The patient developed itching and erythema symptoms during the treatments. The patient learned that metformin could cause adverse reactions such as pruritus and rash by reading the drug label. Then the metformin was stopped by himself and his skin symptoms were relieved within a few days. Due to elevated blood glucose, the patient took metformin again and developed erythema and papules 2 days later, but he didn′t stop the drug. After that, swelling of both lower limbs, accompanied by skin erosion and exudation appeared gradually. Laboratory tests showed that eosinophil count was 0.66×10 9/L. Drug eruptions caused by metformin was considered. All oral hypoglycemic drugs were stopped and symptomatic treatments such as antihistamine and glucocorticoid were given. Five days later, his erythema and papules gradually subsided, and erosive surface of both lower limbs crusted without exudation. Laboratory tests showed eosinophil count 0.06×10 9/L. Dermatitis did not recur after the patient switched to insulin to control blood glucose.

Minocycline is a semi-synthetic tetracycline antibiotic. In recent years, minocycline has been used in the treatment of chronic infections caused by multi-drug resistant bacteria and chronic diseases such as pemphigus and rheumatoid arthritis due to its lower incidence of bacterial resistance and good antibacterial effect. Long term use of oral minocycline can cause skin hyperpigmentation. According to the location, clinical features, and histopathological changes, minocycline-induced skin hyperpigmentation (MISH) is divided into 4 subtypes, but the definition of subtype 4 remains controversial. The incidence of MISH varies greatly in different studies, which may be closely related to factors such as patients′ underlying pathophysiological status, comorbid diseases, dose, and duration of treatment. For those patients who need to take minocycline for a long time, skin color should be observed. Once skin color changes were found, relevant examinations (e.g. skin biopsy) should be carried out in time to make a definite diagnosis as soon as possible. Patients with MISH should pay attention to sun protection. Reducing the dose or stopping minocycline is an effective strategy to improve MISH. Q-switched laser or picosecond laser is recommended to treat pigmentation which can not be recovered after drug withdrawal.

Objective:To compare the clinical effect of Ilizarov frame and unilateral frame in the treatment of tibial segmental bone defect combined with soft tissue defect by open bone transport.Methods:A retrospective case-control study was conducted to analyze the clinical data of 32 patients with tibial segmental bone defect and soft tissue defect-up who underwent open bone transport technique in Tianjin Hospital from August 2008 to August 2016 and obtained complete followed-up. There were 22 males and 10 females, aged 22-64 years (mean, 36.8 years). Nineteen patients in the Ilizarov group showed the mean bone defect length of 7.9 cm and mean soft tissue defect area of 41.4 cm 2. Thirteen patients in the unilateral group showed the mean bone defect length of 7.8 cm and the mean soft tissue defect area of 39.2 cm 2. The results of fracture healing, wound healing time, radiological consolidation index, external fixation index, Association for the Study and Application of the Method of Ilizarov (ASAMI) bone and lower-limb function score and incidence of complications were compared between the groups. Results:All patients were followed up for 24 to 60 months [(32.6±1.3)months]. Bone healing was achieved in all patients. The wound healing time was (2.7±2.3)days in Ilizarov group and (2.4±1.8)days in unilateral group ( P>0.05). The consolidation index was (43.4±8.7)d/cm in Ilizarov group and (45.8±10.3)d/cm in unilateral group ( P>0.05). The external fixation index was (52.6±8.9)d/cm in Ilizarov group and (58.7±12.9)d/cm in unilateral group ( P<0.05). The results of ASAMI bone score was excellent in 10 patients, good in 6, fair in 2 and poor in 1 in Ilizarov group, with the excellent rate of 84%; and excellent in 6 patients, good in 4, fair in 2, and poor in 1 in unilateral group, with the excellent rate of 77%, with insignificant difference between two groups ( P>0.05). The results of ASAMI lower-limb function score was excellent in 8 patients, good in 7, fair in 3, poor in 1 in Ilizarov group, with the excellent rate of 79%; and excellent in 6, good in 3, fair in 3, poor in 1 in unilateral group, with the excellent rate of 69%, with insignificant difference between the two groups ( P>0.05). Incidence of axial deviation was zero in Ilizarov group versus 23% in unilateral group ( P<0.05). After operation, Ilizarov group had refracture in 1 patient and pin site infection in 5, and unilateral group had refracture in 1 patient and pin site infection in 1, showing no significant difference between the two groups ( P>0.05). Conclusions:Tibial segmental bone defect with soft tissue defect can be effectively treated by open bone transport with Ilizarov and unilateral frame. Ilizarov frame has better biomechanical properties and is more convenient for correction of poor postoperative axial alignment.

Objective:To investigate the accuracy and postoperative efficacy of fluoroscopy and CT in measuring the mounting parameters of Taylor Spatial Frame.Methods:Data of patients with peripheral knee deformities who were treated by Taylor Spatial Frame from June 2006 to December 2017 were retrospectively analyzed. According to different measurement methods of mounting parameters, they were divided into fluoroscopy group (mounting parameters were obtained by intraoperative fluoroscopy) and CT group (mounting parameters were obtained by postoperative CT). There were 33 patients (35 segments) in the fluoroscopy group, 23 males (23 segments) and 10 females (12 segments), with an average age of 36.4±11.6 years old. In CT group, there were 30 patients, 19 males and 11 females, with an average age of 36.9±13.8 years. There were 22 cases (24 segments) of high tibial osteotomy, 5 cases (5 segments) of distal femur osteotomy, and 6 cases (6 segments) of both distal femur and high tibial osteotomy. Operation time, external fixation time, the number of electronic prescription and deformity correction time, mechanical axis deviation (MAD), medial proximal tibia angle (MPTA), mechanical lateral distal femoral angle (mLDFA), range of motion (ROM) andhospital for special surgery (HSS) knee functional scores were compared between the two groups.Results:All the 63 patients were followed up for 21.9 months (range, 12-60 months). In fluoroscopy group, operating time was 100.9±9.1 min, electronic prescription number 1.4±0.6, and deformity correction time was 19.4±3.6 days. In CT group, operating time was 79.2±10.8 min, electronic prescription number 1.2±0.4, and deformity correction time was 16.0±4.4 days. The difference of the above indexes between the two groups was statistically significant ( t=8.803, 2.042, 3.440, all P < 0.05). In the fluoroscopy group, the external fixation time was 4.8±0.9 months; MAD was 4.3±2.1 mm; MPTA was 88.5°±1.9°; mLDFA was knee 89.2°; ROM was 122.4°±3.9° and HSS score was 90.0±3.6. In CT group, the external fixation time was 4.6±0.9 months; MAD was 4.0±1.9 mm; MPTA was 87.8°±1.7°; mLDFA was knee 88.6°; ROM was 122.7°±3.4° and HSS score was 91.1±2.9. There was no statistically significant difference in the above indexes between the two groups (all P >0.05). In the fluoroscopy group, 22 segmental deformities were corrected by one electronic prescription, and 13 segmental deformities were corrected by two or more electronic prescriptions. In CT group, 25 segmental deformities were corrected by one electronic prescription, and 5 segmental deformities were corrected by two electronic prescriptions. There was no incision infection and no neurovascular injury in the two groups. Conclusion:Both fluoroscopy and CT scan can obtain the mounting parameters of the Taylor Spatial Frame, and the results of correction of the peripheral deformities of the knee joint are satisfactory. However, CT measurement of the mounting parameters is more accurate which could achieve shorter operation time, and less times of electronic prescriptions.

Objective: To investigate the diagnosis, treatment and prognosis of nonodontogenic periapical lesions and to provide a reference for clinical diagnosis and treatment. Methods: A case of a patient with right upper molar pulp with apical penetration and local occlusion admitted to the West China Stomatological Hospital of Sichuan University was retrospectively analyzed, and the curative effect of microapical surgery and pith preservation was also analyzed. Results : The imaging features of tooth 16 showed periradicular radiolucency combined with local radiopaque lesions around the distal buccal apical area. Endodontic microsurgery was performed under local anesthesia. Soft tissue coverage was observed in the distal buccal apical area during the surgery, and no radiopaque tissue was detected. The distal buccal root apex was cut by 3 mm, and mineral trioxide aggregate was used for root-end backfilling. The postoperative pathological results revealed fibrous connective tissue. One-week recall X-ray examination showed tight root-end backfilling and no periradicular radiolucency; an electrical test of pulp vitality showed positive results. The four-year follow-up showed that there was no discoloration in tooth 16 and no significant difference in thermal and electrical tests of pulp vitality compared with control teeth. Combining the clinical manifestations, imaging features, surgical exploration results and pathological reports, the case was most likely to be cemental hypoplasia. Through the literature review, the treatment and healthy pulp preservation of such cases by endodontic microsurgery under the premise of preserving teeth has not been reported. Conclusion For maxillary posterior teeth with periapical lesions but healthy pulp, accurate estimation of pulp status, endodontic microsurgical exploration and application of bioactive materials can achieve vital pulp preservation while removing the lesions.

Objective:To evaluate the behaviors and eye movement data of the crew in different interface layouts, so as to provide a reference for the interface design of full ocean depth manned submersible.Methods:To simulate the visual stipulations of 7 interface areas in full ocean depth manned submersible by E-prime software, then to record and analyze the correct rates and reaction time of the subjects, and continuously record their eye movement data, i. e. visual dwell time, visual intake frequency, average time of visual intake, average pupil diameter, saccade amplitude, average saccade acceleration, and average speed of saccade.Results:The interface areas were ranked according to the correct rates from the highest to the lowest: 3#, 6#, 7#, 2#, 1#, 4#, 5#. The interface areas were ordered according to the reaction time from the shortest to the longest: 3#, 2#, 4#, 6#, 7#, 1#, 5#. The visual dwell time of 3# and 4# areas were significantly longer than others with statistical significance ( P<0.05 or P<0.01). The visual intake frequency of 3# and 4# areas were significantly higher than others with statistical significance ( P<0.05 or P<0.01). The average time of visual intake of 3# area was significantly longer than others with statistical significance ( P<0.05 or P<0.01). The average pupil diameter of 6# and 7# areas were significantly smaller than others, except 3# area, with statistical significance ( P<0.05 or P<0.01). There was no statistical difference in the comparison of saccade amplitude, average saccade acceleration, and average speed of saccade among all the areas ( P>0.05). Conclusion:When designing the man-machine interface in full ocean depth manned submersible, the key information should be displayed in 3# and 4# areas, primary information should be displayed in 1#, 2#, and 5# areas, and the secondary information should be displayed in 6# and 7# areas.