Objective:To explore the potential categories of emotional behavior and its influencing factors in obese junior high school students.Methods:A total of 1 841 obese junior high school students were selected by convenient sampling method and surveyed by basic information scale, co-residence (CRS), online time (OT), outdoor exercise intensity (OEI) and subjective socioeconomic status scale for adolescent(SSS-A) score, Chinese version of perceived stress scale(CPSS), short grit scale(Grit-S), adolescent mental health literacy assessment questionnaire(AMHLAQ) and strengths and difficulties questionnaire (SDQ).Statistical analysis were conducted by SPSS 26.0 software and Mplus 8.3 software.The latent class analysis was used to group the students according to the SDQ scores, and the disordered multivariate Logistic regression was used to explore the influencing factors of each category.Results:There were 4 latent categories of emotional behavior in obese junior high school students, which were anxiety-independence group (22.49%), depression-passive group (28.25%), contradiction-affinity group(24.72%) and stability-sociability group (24.55%).Students with low CPSS, Grit-S, AMHLAQ and SSS-A scores were more likely to enter the anxiety-independence group with stability-sociability group as a reference( B=0.893, OR=2.442, 95% CI=1.083-5.509; B=-1.645, OR=0.193, 95% CI=0.067-0.553; B=0.030, OR=1.031, 95% CI=1.007-1.055; B=-0.177, OR=0.883, 95% CI=0.812-0.953; B=-0.066, OR=0.934, 95% CI=0.899-1.081; B=-0.302, OR=0.739, 95% CI=0.695-0.786), living with one parent, and having low CPSS, Grit-S, and AMHLAQ scores; students with OT<1 h OEI≥4 d and low Grit-S total score were more likely to enter the depression-passive group( B=0.930, OR=2.715, 95% CI=1.206-5.945; B=-0.447, OR=0.572, 95% CI=0.363-0.953; B=-0.201, OR=0.818, 95% CI=0.793-0.844); students with high CPSS total score and low Grit-S and AMHLAQ total scores were easy to enter the contradiction-affinity group( B=-1.752, OR=0.174, 95% CI=0.054-0.562; B=0.087, OR=1.091, 95% CI=1.065-1.118; B=-0.273, OR=0.783, 95% CI=0.752-0.871; B=-0.036, OR=0.965, 95% CI=0.938-0.991); OEI≥4 d and OT <1 h were the protective factors of the stability-sociability group ( P<0.05). Conclusion:There is heterogeneity in the emotional and behavioral characteristics of obese junior high school students, which can be divided into four categories.Living with parents, controlling the length of time on the internet, maintaining a certain intensity of outdoor sports, strengthening stress management and perseverance quality training, and improving mental health literacy can help prevent emotional and behavioral problems.

Objective To analyze the efficacy of the"sandwich"technique for treating varicocele(VC).Methods A total of 310 patients with VC(365 affected veins)were selected and divided into interventional treatment group and non-interventional treatment group.The baseline data,hospitalization data,and 6-month follow-up data of the two groups were analyzed.Results The age of patients in the interventional treatment group was significantly lower than that in the non-interventional treatment group(P＜0.05).The surgical time and hospital stay in the interventional treatment group were significantly lower than those in the non-interventional treatment group(P＜0.05).In the non-interventional treatment group,two patients experienced surgical site infections,and one patient opted for interventional treatment due to recurrence after non-interventional treatment.After surgery,the diameter of the spermatic vein significantly decreased in both the interventional and non-interventional treatment(P＜0.05).Conclusion The"sandwich"technique(embolization coil combined with foam sclerotherapy)is an effective treatment for VC.

Objective:To explore the potential categories of emotional behavior and its influencing factors in obese junior high school students.Methods:A total of 1 841 obese junior high school students were selected by convenient sampling method and surveyed by basic information scale, co-residence (CRS), online time (OT), outdoor exercise intensity (OEI) and subjective socioeconomic status scale for adolescent(SSS-A) score, Chinese version of perceived stress scale(CPSS), short grit scale(Grit-S), adolescent mental health literacy assessment questionnaire(AMHLAQ) and strengths and difficulties questionnaire (SDQ).Statistical analysis were conducted by SPSS 26.0 software and Mplus 8.3 software.The latent class analysis was used to group the students according to the SDQ scores, and the disordered multivariate Logistic regression was used to explore the influencing factors of each category.Results:There were 4 latent categories of emotional behavior in obese junior high school students, which were anxiety-independence group (22.49%), depression-passive group (28.25%), contradiction-affinity group(24.72%) and stability-sociability group (24.55%).Students with low CPSS, Grit-S, AMHLAQ and SSS-A scores were more likely to enter the anxiety-independence group with stability-sociability group as a reference( B=0.893, OR=2.442, 95% CI=1.083-5.509; B=-1.645, OR=0.193, 95% CI=0.067-0.553; B=0.030, OR=1.031, 95% CI=1.007-1.055; B=-0.177, OR=0.883, 95% CI=0.812-0.953; B=-0.066, OR=0.934, 95% CI=0.899-1.081; B=-0.302, OR=0.739, 95% CI=0.695-0.786), living with one parent, and having low CPSS, Grit-S, and AMHLAQ scores; students with OT<1 h OEI≥4 d and low Grit-S total score were more likely to enter the depression-passive group( B=0.930, OR=2.715, 95% CI=1.206-5.945; B=-0.447, OR=0.572, 95% CI=0.363-0.953; B=-0.201, OR=0.818, 95% CI=0.793-0.844); students with high CPSS total score and low Grit-S and AMHLAQ total scores were easy to enter the contradiction-affinity group( B=-1.752, OR=0.174, 95% CI=0.054-0.562; B=0.087, OR=1.091, 95% CI=1.065-1.118; B=-0.273, OR=0.783, 95% CI=0.752-0.871; B=-0.036, OR=0.965, 95% CI=0.938-0.991); OEI≥4 d and OT <1 h were the protective factors of the stability-sociability group ( P<0.05). Conclusion:There is heterogeneity in the emotional and behavioral characteristics of obese junior high school students, which can be divided into four categories.Living with parents, controlling the length of time on the internet, maintaining a certain intensity of outdoor sports, strengthening stress management and perseverance quality training, and improving mental health literacy can help prevent emotional and behavioral problems.

Objective:To investigate the differential expression profiles of long non-coding RNAs(LncRNAs) and messenger RNAs(mRNAs) regulated by soluble dipeptidyl peptidase-4(sDPP4) during vascular smooth muscle cell calcification, and to explore the potential underlying calcification mechanisms.Methods:DPP4 levels in blood vessels and peripheral blood of diabetic patients were measured using Western blotting(WB) and real-time quantitative PCR(RT-qPCR). A cellular calcification model was established by treating human aortic vascular smooth muscle cells(HASMCs) with sDPP4. The effects of sDPP4 on HASMCs were assessed by WB, RT-qPCR, alizarin red staining, and calcium content determination. High-throughput sequencing was performed to analyze the differential expression profiles of LncRNA and mRNA following sDPP4 treatment. Among them, LncRNA ENST00000540293, which exhibited the most pronounced downregulation and was located adjacent to the matrix metalloproteinase-1(MMP-1) gene, was selected for further investigation. The osteogenic transdifferentiation of HASMCs after silencing LncRNA ENST00000540293 was evaluated using WB, RT-qPCR, alizarin red staining, and immunofluorescence-based cytoskeletal staining.Results:DPP4 expression was significantly elevated in both blood vessels and peripheral blood of diabetic patients. sDPP4 stimulation upregulated the protein levels of osteopontin(OPN) and runt-related transcription factor 2(RUNX2) in HASMCs, enhanced alizarin red staining, and increased intracellular calcium deposition. RNA sequencing revealed significant downregulation of LncRNA ENST00000540293 following sDPP4 exposure, while GO and pathway analysis indicated a marked increase in extracellular matrix binding activity(GO: 0050840). Silencing LncRNA ENST00000540293 suppressed α-smooth muscle actin(α-SMA) expression, promoted OPN and RUNX2 expression, increased calcification as shown by positive alizarin red staining, and cytoskeletal staining demonstrated osteogenic transdifferentiation of HASMCs, accompanied by a significant rise in MMP-1 protein level.Conclusion:sDPP4 promotes osteogenic transdifferentiation of HASMCs, potentially by downregulating LncRNA ENST00000540293. MMP-1 may be a potential target regulated by LncRNA ENST00000540293.

Objective:To investigate the differential expression profiles of long non-coding RNAs(LncRNAs) and messenger RNAs(mRNAs) regulated by soluble dipeptidyl peptidase-4(sDPP4) during vascular smooth muscle cell calcification, and to explore the potential underlying calcification mechanisms.Methods:DPP4 levels in blood vessels and peripheral blood of diabetic patients were measured using Western blotting(WB) and real-time quantitative PCR(RT-qPCR). A cellular calcification model was established by treating human aortic vascular smooth muscle cells(HASMCs) with sDPP4. The effects of sDPP4 on HASMCs were assessed by WB, RT-qPCR, alizarin red staining, and calcium content determination. High-throughput sequencing was performed to analyze the differential expression profiles of LncRNA and mRNA following sDPP4 treatment. Among them, LncRNA ENST00000540293, which exhibited the most pronounced downregulation and was located adjacent to the matrix metalloproteinase-1(MMP-1) gene, was selected for further investigation. The osteogenic transdifferentiation of HASMCs after silencing LncRNA ENST00000540293 was evaluated using WB, RT-qPCR, alizarin red staining, and immunofluorescence-based cytoskeletal staining.Results:DPP4 expression was significantly elevated in both blood vessels and peripheral blood of diabetic patients. sDPP4 stimulation upregulated the protein levels of osteopontin(OPN) and runt-related transcription factor 2(RUNX2) in HASMCs, enhanced alizarin red staining, and increased intracellular calcium deposition. RNA sequencing revealed significant downregulation of LncRNA ENST00000540293 following sDPP4 exposure, while GO and pathway analysis indicated a marked increase in extracellular matrix binding activity(GO: 0050840). Silencing LncRNA ENST00000540293 suppressed α-smooth muscle actin(α-SMA) expression, promoted OPN and RUNX2 expression, increased calcification as shown by positive alizarin red staining, and cytoskeletal staining demonstrated osteogenic transdifferentiation of HASMCs, accompanied by a significant rise in MMP-1 protein level.Conclusion:sDPP4 promotes osteogenic transdifferentiation of HASMCs, potentially by downregulating LncRNA ENST00000540293. MMP-1 may be a potential target regulated by LncRNA ENST00000540293.

Objective To analyze the efficacy of the"sandwich"technique for treating varicocele(VC).Methods A total of 310 patients with VC(365 affected veins)were selected and divided into interventional treatment group and non-interventional treatment group.The baseline data,hospitalization data,and 6-month follow-up data of the two groups were analyzed.Results The age of patients in the interventional treatment group was significantly lower than that in the non-interventional treatment group(P＜0.05).The surgical time and hospital stay in the interventional treatment group were significantly lower than those in the non-interventional treatment group(P＜0.05).In the non-interventional treatment group,two patients experienced surgical site infections,and one patient opted for interventional treatment due to recurrence after non-interventional treatment.After surgery,the diameter of the spermatic vein significantly decreased in both the interventional and non-interventional treatment(P＜0.05).Conclusion The"sandwich"technique(embolization coil combined with foam sclerotherapy)is an effective treatment for VC.

The drafting of Zhejiang Mental Health Regulation has started since the promulgation of the Mental Health Law of the People's Republic of China in the year of 2013. Based on the preliminary investigation and the practice of mental health work in Zhejiang Province,this paper introduces the contents and characteristics of the regulation from six aspects,which are the legislative purpose and scope of application, the responsibilities of the government and departments, the construction of mental health service system,management of mental health services and consulting institutions,prevention,treatment and rehabilitation of mental disorders, and measures necessary to implement the regulation.

OBJECTIVE: To analyze the usage of mental health assistance hotline during COVID-19 in Zhejiang province from January 25th to February 29th 2020, and summarize the characteristics of the demand for mental health services and the dynamic changes of public mental health status during COVID-19 pandemic. METHODS: Both quantitative and qualitative methods were used. The calls related to pandemic were divided into four categories: medical, psychological, information and the others. The secondary categories of psychological calls were determined by text analysis. The number of calls were calculated weekly and the number of various types of calls over time were analyzed. We used stratified random sampling method to extract 600 cases of all kinds of calls related to pandemic and conducted a semantic analysis, through marking new, similar combination to form a feature set, then summed up the call content characteristics of each stage. Two hundred callers were followed up to understand how they felt about the call process in four aspects: the waiting time, call duration, the degree of problem-solving and the way to end the call. RESULTS: In a total of 13 746 calls, 8978 were related to pandemic, among which 12.59%(1130/8978) were about medical issues, 26.50%(2379/8978) were about mental health, 27.18%(2440/8978) were about information regarding the pandemic and 33.74%(3029/8978) were about other pandemic related issues. Pandemic situation, relevant policy release, frequency of advertising campaigns were predictors of the number of calls per day during the pandemic (<0.05 or <0.01). The number of calls differed by gender and identities of callers (both <0.05). Finally 181 callers accepted telephone follow-up. Among them, 51.38%(93/181) of the callers thought that the waiting time was too long, 33.15%(60/181) of the callers thought that the call time was insufficient, 80.66%(146/181) of callers believed that the hotline could partially or completely resolve their concerns, and 39.23%(71/181) of the callers said the operator proposed to end the call. CONCLUSIONS s The changes of the number and content of the mental health assistance hotline calls reflected that the public mental health status experienced four stages during the pandemic: confusion, panic, boredom, and adjustment. The specialized mental health assistance hotlines should be further strengthened, and the efficiency should be improved. Mental health interventions should be tailored and adopted according to the characteristics of the public mental health status at different stages of the pandemic.
China ; epidemiology ; Coronavirus Infections ; epidemiology ; Hotlines ; statistics & numerical data ; Humans ; Mental Health ; statistics & numerical data ; Pandemics ; statistics & numerical data ; Pneumonia, Viral ; epidemiology ; Public Health ; statistics & numerical data

OBJECTIVE: To explore the effects of three dentine desensitizers on the surface morphology of freshly exposed dentin and to evaluate their occlusion effects on dentinal tubules using scanning electron microscope (SEM). METHODS: A total of 16 isolated human premolar samples, which were prepared to expose dentine, were randomly divided into a control group (n=4), a Hybrid Coat group (n=4), a Prime & Bond NT group (n=4), and a anti-sensitivity toothpaste group (n=4). After treatment with dentine desensitizers, one half of the samples in each group were vertically cleaved. Finally, the surfaces and cross sections of the samples were observed by SEM. RESULTS: The exposed tubule was almost occluded in the Hybrid Coat group and the Prime & Bond NT group, while only the majority of tubules could be sealed in the anti-sensitivity toothpaste group. The cross-section images showed that sediments were visible in all groups except the control group. CONCLUSION Hybrid Coat and Prime & Bond NT are able to effectively seal tubules, while the effect of anti-sensitivity toothpaste is slightly poor.
Dentin ; ultrastructure ; Dentin Desensitizing Agents ; pharmacology ; Dentin Permeability ; drug effects ; Humans ; Microscopy, Electron, Scanning ; Polymethacrylic Acids ; pharmacology ; Toothpastes ; pharmacology

Objective To analyze the features of cervical vertebra X-ray examination and transcranial Doppler (TCD) in children with cervical vertigo, and investigate the mechanism of the disease. Methods X-ray examination of cervical vertebra and TCD were performed on 80 children with cervical vertigo, and the features of the results were analyzed. Results The abnormal results of cervical X-ray examination mainly showed atlantoaxial joint or/and C2, C3 vertebral joint disorders, some of which combined with cervical instability, cervical curvaturestraightened and even reverse curvature, cervical scoliosis. The abnormal results of TCD showed the blood flow velocity in single or multiple branch of artery increased mainly, some of which decreased or became asymmetrial. Conclusion The cervical vertigo in children may result from various kinds of disorders of the atlas, axis or/and C2, C3 vertebrae.