Thioredoxin-interacting protein(TXNIP) inhibitors can inhibit the apoptosis of pancreatic β cells, while dual-specificity tyrosine-phosphoryation-regulated kinase 1A (DYRK1A) inhibitors can promote the proliferation of pancreatic β cells. This study used 4-benzopyrimidine as the starting material to design and synthesize benzopyrimidine-7-azaindole scaffold derivatives targeting TXNIP/DYRK1A by linking it with variously substituted 7-azaindoles via carbon chains of different lengths. A total of 12 novel quinazoline-7-azaindole derivatives were designed and synthesized, their structures confirmed by 1H NMR and ESI-MS, and their content was determined by HPLC. Pharmacological activity assays demonstrated that all 12 compounds exhibited inhibitory effects on β-cell apoptosis, with YN-3 and YN-6 showing the strongest activity, achieving a cell survival rate exceeding 75% (compared to 43.08% in the control group). Proliferation-promoting experiments revealed that most compounds exhibited proliferative activity, with YN-1–YN-3 and YN-11 demonstrating proliferation enhancement rates greater than 125%. YN-3exhibited the strongest combined anti-apoptotic and proliferation-promoting activities and may represent a promising new chemical entity for antidiabetic drug development.

This paper summarized training status of the Master of Nursing Specialist (MNS) at home and abroad,described and analyzed the cultivation program at home and abroad,and discussed the problems about cultivation objective, enrollment, course system, specialty orientation, clinical training model, teaching method, connotation of practical ability, teachers strength, evaluation and assessment in cultivation of MNS in China. Then MNS training program is proposedso as to provide references for the future cultivation program of MNS in China.

Objective To study the HLA match rate of 222 living-related donors for kidney transplantation, and to give suggestions for clinical selection of suitable donors and recipients. Methods We analyzed the HLA match rate of 222 kidney transplantations from living relative donors from April 2006 to December 2008. There were 168 male recipients and 54 female recipients. The ages of 222 recipients ranged from 10 to 58. There were 133 male donors and 89 female donors. The ages of 222 recipients ranged from 21 to 64.Results The HLA-A, B, DR, DR, antigens of 87 kidney transplantations from living parental donors were half-matched, of which 14 were higher than half-matched. The HLA-A, B, DR, DQ antigens of 7 kidney transplantations from living children donors were half-matched, including 2 cases higher than half-matched.Among 56 kidney transplantations from living sibling donors, 12 cases were totally mated, 34 cases were half-matched, and the rest were less than half-matched or mismatched. Among 72 kidney transplantations from other living relative donors, 20 cases were higher than half-matched and 5 cases were completely mismatched. More than 4 HLA antigens in 6 cases were matched, but not half-matched. Three HLA antigens or less were matched in 41 cases. Conclusion The HLA match rates from living parental, children, or sibling donors were higher than other relative donors.

Kidney transplantation has become the optimal treatment for end-stage renal disease. However, the demand for kidney exceeds the available supply. In last years, living-related kidney transplantation has progressively increased because of the less rejection, the higher achievement ratio and so on. From 2007 to 2008 ,our hospital succeed in 196 living-related kidney transplantation based on the previous work.