Objective To investigate the impact of closed negative pressure drainage in combination with Ilizarov transverse tibial bone transport on oxidative stress and inflammatory response in patients with severe diabetic foot.Methods A total of 60 patients with severe diabetic foot who were admitted to the hospital from July 2019 to March 2023 were recruited as the research subjects.These patients were randomly allocated into a control group(n=30)and an observation group(n=30).The control group received Ilizarov tibial transverse bone transport alone,whereas the observation group was treated with closed negative pressure drainage in conjunction with Ilizarov tibial transverse bone transport.The following parameters were compared between the two groups of patients:the reduction rate of wound size,the healing rate,growth factors[Epidermal Growth Factor(EGF),Transforming Growth Factor(TGF),Vascular Endothelial Growth Factor(VEGF)],oxidative stress indicators[Advanced Protein Oxidation Products(AOPP),Malondialdehyde(MDA),Superoxide Dismutase(SOD)],inflammatory factors[Procalcitonin(PCT),Interleukin-18(IL-18),C-reactive Protein(CRP)],adverse reactions,and the amputation rate.Results The shrinkage rate and healing rate of patients in the observation group were both significantly higher than those in the control group(P<0.05).Post-treatment,the improvement in growth factor levels,including EGF,TGF,and VEGF,in the observation group was more pronounced compared to that in the control group(P<0.05).Regarding serum AOPP,MDA,and SOD levels,the improvement in the observation group was superior to that in the control group(P<0.05).Moreover,the improvement in inflammatory factor levels such as serum PCT,IL-18,and CRP in the observation group was more notable than that in the control group(P<0.05).The incidence of adverse reactions such as redness,swelling,pain,and bleeding in the observation group was 6.67%.When compared with the incidence of 11.67%in the control group,no statistically significant difference was observed(P>0.05).The amputation rate of patients in the observation group was 3.33%,which was significantly lower than 23.33%in the control group(P<0.05).Conclusions Closed negative pressure drainage combined with Ilizarov tibial transverse bone transport demonstrates better efficacy in the treatment of severe diabetic foot.This treatment modality can effectively promote wound healing,enhance oxidative stress regulation,and inhibit the inflammatory response,presenting a relatively high safety profile.Therefore,the application of closed negative pressure drainage combined with Ilizarov tibial transverse bone transport in the treatment of severe diabetic foot merits clinical reference and promotion.

Objective: To investigate the value of the Chinese visceral adiposity index (CVAI) in predicting hypertension risk, so as to provide a tool for the early assessment of hypertension risk. Methods: Health examination individuals aged ≥18 years were selected from four medical institutes in Jinhua City, Zhejiang Province in 2022. Data on basic information, lifestyle, disease history, body mass index, waist circumference, blood pressure, and blood biochemical indicators were collected through questionnaire surveys and physical examinations. CVAI was calculated to assess levels of visceral fat accumulation, divided by quartiles into Q1, Q2, Q3, and Q4 groups. The relationship between CVAI and hypertension was analyzed using a multivariable logistic regression model, and their dose-response relationship was examined using a restricted cubic spline model. The value of CVAI in predicting hypertension risk was evaluated using receiver operating characteristic (ROC) curve. Results: A total of 23 791 individuals were enrolled, with a median age of 68.00 (interquartile range, 14.00) years. Among them, 10 178 (42.78%) were males and 13 613 (57.22%) were females. The median CVAI was 111.40 (interquartile range, 48.23). Hypertension was identified in 15 563 cases, with a prevalence of 65.42%. After adjusting for lifestyle, disease history, and blood biochemical indicators, the multivariable logistic regression analysis revealed that hypertension risk in the CVAI Q2, Q3, and Q4 groups were 2.012 (95%CI: 1.865-2.170), 3.059 (95%CI: 2.826-3.311), and 5.099 (95%CI: 4.672-5.565) times that of the Q1 group, respectively. The restricted cubic spline model revealed a non-linear relationship between CVAI and hypertension risk (Pnon linearity<0.05). Hypertension risk increased more rapidly when the CVAI was ≥81.03. The area under the ROC curve for CVAI in predicting hypertension risk was 0.691, with an optimal cutoff value of 106.01, which falls within the Q2 group. Conclusions There was a nonlinear dose-response relationship between CVAI and hypertension. CVAI can predict the risk of hypertension, and 106.01 can serve as an early warning threshold for risk screening.

Recent studies have shown that lysosomal dysfunction is closely related to Alzheimer's disease (AD). Lysosomal ion channels, such as transient receptor potential channel mucolipin-1, two-pore channel, and transmembrane protein 175, play an important role in maintaining lysosomal stability and reducing pathological protein accumulation, and are expected to become new targets for AD intervention and treatment. This article reviews the research progress on basic characteristics, physiological functions of the above lysosomal ion channels and their roles in AD, aiming to provide new targets and research perspectives for AD treatment.

Objective To investigate the impact of closed negative pressure drainage in combination with Ilizarov transverse tibial bone transport on oxidative stress and inflammatory response in patients with severe diabetic foot.Methods A total of 60 patients with severe diabetic foot who were admitted to the hospital from July 2019 to March 2023 were recruited as the research subjects.These patients were randomly allocated into a control group(n=30)and an observation group(n=30).The control group received Ilizarov tibial transverse bone transport alone,whereas the observation group was treated with closed negative pressure drainage in conjunction with Ilizarov tibial transverse bone transport.The following parameters were compared between the two groups of patients:the reduction rate of wound size,the healing rate,growth factors[Epidermal Growth Factor(EGF),Transforming Growth Factor(TGF),Vascular Endothelial Growth Factor(VEGF)],oxidative stress indicators[Advanced Protein Oxidation Products(AOPP),Malondialdehyde(MDA),Superoxide Dismutase(SOD)],inflammatory factors[Procalcitonin(PCT),Interleukin-18(IL-18),C-reactive Protein(CRP)],adverse reactions,and the amputation rate.Results The shrinkage rate and healing rate of patients in the observation group were both significantly higher than those in the control group(P<0.05).Post-treatment,the improvement in growth factor levels,including EGF,TGF,and VEGF,in the observation group was more pronounced compared to that in the control group(P<0.05).Regarding serum AOPP,MDA,and SOD levels,the improvement in the observation group was superior to that in the control group(P<0.05).Moreover,the improvement in inflammatory factor levels such as serum PCT,IL-18,and CRP in the observation group was more notable than that in the control group(P<0.05).The incidence of adverse reactions such as redness,swelling,pain,and bleeding in the observation group was 6.67%.When compared with the incidence of 11.67%in the control group,no statistically significant difference was observed(P>0.05).The amputation rate of patients in the observation group was 3.33%,which was significantly lower than 23.33%in the control group(P<0.05).Conclusions Closed negative pressure drainage combined with Ilizarov tibial transverse bone transport demonstrates better efficacy in the treatment of severe diabetic foot.This treatment modality can effectively promote wound healing,enhance oxidative stress regulation,and inhibit the inflammatory response,presenting a relatively high safety profile.Therefore,the application of closed negative pressure drainage combined with Ilizarov tibial transverse bone transport in the treatment of severe diabetic foot merits clinical reference and promotion.

Recent studies have shown that lysosomal dysfunction is closely related to Alzheimer's disease (AD). Lysosomal ion channels, such as transient receptor potential channel mucolipin-1, two-pore channel, and transmembrane protein 175, play an important role in maintaining lysosomal stability and reducing pathological protein accumulation, and are expected to become new targets for AD intervention and treatment. This article reviews the research progress on basic characteristics, physiological functions of the above lysosomal ion channels and their roles in AD, aiming to provide new targets and research perspectives for AD treatment.

Objective:To explore the protective mechanism of tea polyphenols (TP) on mouse oral cancer.Methods:A total of 50 mice were divided into control group, model group, TP group, Selisistat group, TP+ Selisistat group, with 10 mice in each group. The control group was gavaged with physiological saline, while the model group, TP group, Selisistat group, and TP+ Selisistat group were gavaged with 300 mg/L 4-NQO to establish a mouse oral cancer model. Physiological saline, 200 mg/kg TP, 0.01 mg/kg Selisistat, and 200 mg/kg TP+ 0.01 mg/kg Selisistat were gavaged respectively. The weight changes of each group of mice were compared; HE staining was used to observe the morphology of mouse oral tumor tissue; Enzyme linked immunosorbent assay was used to detect the levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in serum; Immunoblotting and immunohistochemistry were used to detect the expression of silencing information regulatory factor (Sirt1) and nuclear factor E2 related factor 2 (Nrf2) proteins in mouse oral tissues.Results:Compared with the control group, the model group mice had a decrease in body weight [(23.19±1.36)g], a decrease in serum SOD level [(91.64±8.75)U/ml], an increase in MDA level [(5.18±0.46)nmol/ml], a decrease in Sirt1 (0.38±0.05) and Nrf2 (0.36±0.05) protein expression in oral tissue, and an increase in Nrf2 acetylation level (0.84±0.11) (all P<0.05). Compared with the model group, the TP group mice had an increase in body weight [(25.28±1.25)g], elevated serum SOD levels [(121.24±10.68)U/ml], decreased MDA levels [(3.89±0.42)nmol/ml], increased expression of Sirt1 (0.61±0.09) and Nrf2 (0.58±0.06) proteins in oral tissue, and decreased Nrf2 protein acetylation levels (0.39±0.05); The Selisistat group mice showed a decrease in body weight [(21.41±1.07)g], a decrease in serum SOD levels [(72.16±7.43)U/ml], an increase in MDA levels [(5.87±0.41)nmol/ml], a decrease in Sirt1 (0.23±0.04) and Nrf2 protein (0.24±0.03) expression in oral tissue, and an increase in Nrf2 acetylation levels (1.12±0.14) ( P<0.05). The body weight [(23.32±1.27)g], serum SOD levels [(92.58±8.13)U/ml], and oral Sirt1 (0.41±0.06) and Nrf2 (0.38±0.05) protein expression in the TP+ Selisistat group mice were higher than those in the Selisistat group, while MDA [(5.11±0.38)nmol/ml] and Nrf2 acetylation levels (0.82±0.09) were lower than those in the Selisistat group (all P<0.05). Conclusions:Tea polyphenols can alleviate oral tissue damage and alleviate oxidative stress in mice with oral cancer, and their mechanism may be related to the upregulation of the Sirt1/Nrf2 pathway.

Objection:To evaluate the effects of the miniscrew supported removable palatal arch for maxillary molar distalization.Methods:33 patients with mild to moderate crowding teeth and maxillary protrusion were treated with the miniscrew supported remova-ble palatal arch.The lateral cephalograms and dental plaster models were taken at the beginning of the treatment(T0)and at the end of molar distalization(T1).The multicentre variables were measured and compared with statistical software.Results:Cephalometrics showed that the average distance(mm)of the firs molar distalization was 3.6(P＜0.001),molar distal tipping was 6°(P＜0.001)and molar intrusion(mm)was 0.6(P＜0.05).The measurements of right and left molar distalization on plaster models were 4.2 mm(P＜0.001)and 4.0 mm(P＜0.001)respectively,the width of dental arch was increased by 3.1 mm(P＜0.05).The data of distalization of the first molar showed no statistical difference between right and left side on plaster cast and between model and cephalometric meas-urements.On the right side the angle between the mesial-distal tips line and the middle line was increased by 1.6°(P＞0.05),on the left by 4.8°(P＜0.05).Conclusion:The none extraction therapy for mild to moderate crowding teeth and maxillary protrusion can be realized by miniscrew supported removable palatal arch distalization appliance.

Objective:To explore the best evidence of non-surgical management of stress urinary incontinence in women, so as to provide evidence for effective prevention and treatment of related diseases.Methods:According to the "6S" classification model of evidence-based retrieval resources, the researches on non-surgical management of female patients with stress urinary incontinence were retrieved in UpToDate, BMJ Best Practice, major guideline websites, professional association websites and other Chinese and English databases, including clinical decision-making, evidence-based guidelines, systematic reviews, expert consensus and randomized controlled trials. The search period was from establishment of databases to April 30, 2023.Results:A total of 13 articles were included, including three clinical decisions, five guidelines, one expert consensus and four systematic evaluations. The best evidence summarized included 27 items in four aspects, such as screening and evaluation, lifestyle behavior management, functional training and medication treatment.Conclusions:The best evidence for non-surgical management of female patients with stress urinary incontinence summarized in this study can provide guidance for clinical medical staff to manage female stress urinary incontinence scientifically and normatively. Medical personnel can apply this evidence according to the national conditions of China and patients' own conditions.

Purpose: The risk stratification of pediatric anaplastic large cell lymphoma (ALCL) has not been standardized. In this study, new risk factors were included to establish a new risk stratification system for ALCL, and its feasibility in clinical practice was explored. Materials and Methods: On the basis of the non-Hodgkin’s lymphoma Berlin–Frankfurt–Munster 95 (NHL-BFM-95) protocol, patients with minimal disseminated disease (MDD), high-risk tumor site (multiple bone, skin, liver, and lung involvement), and small cell/lymphohistiocytic (SC/LH) pathological subtype were enrolled in risk stratification. Patients were treated with a modified NHL-BFM-95 protocol combined with an anaplastic lymphoma kinase inhibitor or vinblastine (VBL). Results: A total of 136 patients were enrolled in this study. The median age was 8.8 years. The 3-year event-free survival (EFS) and overall survival of the entire cohort were 77.7% (95% confidence interval [CI], 69.0% to 83.9%) and 92.3% (95% CI, 86.1% to 95.8%), respectively. The 3-year EFS rates of low-risk group (R1), intermediate-risk group (R2), and high-risk group (R3) patients were 100%, 89.5% (95% CI, 76.5% to 95.5%), and 67.9% (95% CI, 55.4% to 77.6%), respectively. The prognosis of patients with MDD (+), stage IV cancer, SC/LH lymphoma, and high-risk sites was poor, and the 3-year EFS rates were 45.3% (95% CI, 68.6% to 19.0%), 65.7% (95% CI, 47.6% to 78.9%), 55.7% (95% CI, 26.2% to 77.5%), and 70.7% (95% CI, 48.6% to 84.6%), respectively. At the end of follow-up, one of the five patients who received maintenance therapy with VBL relapsed, and seven patients receiving anaplastic lymphoma kinase inhibitor maintenance therapy did not experience relapse. Conclusion This study has confirmed the poor prognostic of MDD (+), high-risk site and SC/LH, but patients with SC/LH lymphoma and MDD (+) at diagnosis still need to receive better treatment (ClinicalTrials.gov number, NCT03971305).

Objective : To discuss the effectiveness and mechanism for movement of maxillary buccally transposed canines by using a door-shaped individualized dental archwire mechanic and to provide a reference for clinicians. Methods: Eight patients with unilateral maxillary transposed canines were enrolled. All patients were treated with door-shaped individualized archwires. Before treatment (T1) and after the crowns of the transposed canines were moved to the right buccal positions in the dental arch during the treatment (T2), orthopantomograms were taken both at T1 and T2 to compare the linear changes (distance changes of the crown and root apex) and angular changes to study the mechanisms of tooth movement. The probing depth and buccal crown height were measured using a periodontal probe to compare periodontal changes before treatment (T1) and after treatment (T3) between the transposed canines and contralateral canines. Results: All eight transposed canines were successfully brought back to their normal dental arch position but were made more buccal by using the door-shaped individualized dental archwire, with a mean of (11.5 ± 2.7) months. The average overall duration was (28.3 ± 4.7) months. The crown distance changes of the canines from T1 to T2 (8.1 mm) were greater than those of the root apexes (1.5 mm) (P<0.05). The mean angulation changes of the long axes of the canines were 17.5°. There was no significant difference in the depth of periodontal measurement and buccal crown height measurement between T1 and T3 (P>0.05). Conclusion The buccal movement of maxillary transposed canines under a door-shaped individualized dental archwire was effective and feasible. The movement pattern under this mechanism was controlled tipping.