Objective To develop a machine learning(ML)-based prediction model for assessing the therapeutic effects of resveratrol(RES)on the pathological damage of acute pancreatitis(AP),and to optimize RES administration strategies for AP through validation using an animal model.Methods AAn ML-based prediction model was constructed using published data.Interpretability analysis was applied to identify high-efficacy zones within the parameter space of administration dose and frequency,which was followed by rigorous screening to select the optimal dosing strategy that balanced therapeutic efficacy and experimental feasibility.A total of 32 C57BL/6 mice were randomly assigned to 4 groups(n=8 per group),including a control group(Ctrl),an AP model group induced by caerulein(CER)and referred to as CER-AP,a treatment group receiving RES via intraperitoneal injection(RES i.p.),and a treatment group receiving RES via intragastric gavage(RES i.g.).The Ctrl group received intraperitoneal injection of normal saline.The CER-AP and the treatment groups were induced with 10 intraperitoneal injections of CER at 50 μg/kg.RES was administered to the RES i.p.and RES i.g.groups according to the optimal dose and timing predicted by the ML model.Blood and tissue samples were collected 12 hours after the experiment started.Results The gradient boosting decision tree model,optimized via Hyperopt,yielded the best performance,predicting that the optimal dose and administration frequency were 19.992 mg/kg and 3.828 times,respectively.Accordingly,a regimen of 20 mg/kg RES,administered four times,was used in the animal experiments.Compared with the Ctrl group,the CER-AP group exhibited higher pancreatic pathology scores and elevated levels of serum amylase,lipase,pancreatic myeloperoxidase,and trypsin,with all differences reaching statistical significance(all P<0.05).The administration of 20 mg/kg RES via both intraperitoneal injection and intragastric gavage mitigated pancreatic inflammatory cell infiltration and necrosis,improved the overall pathology score,and reduced serum amylase,lipase,and pancreatic myeloperoxidase levels to varying degrees(all P<0.05).Conclusion A regimen of 20 mg/kg RES administered four times effectively alleviates the severity of CER-induced AP.The therapeutic benefits appear to arise from a multi-target regulatory network that simultaneously suppresses inflammatory cascades,mitigates oxidative stress,and reduces apoptosis,thereby reducing pancreatic tissue damage and systemic inflammatory responses.

Objective　To compare the changes in the genetic diversity of Anopheles minimus through the research on the population genetic characteristics of Anopheles minimus between different years in Nabang Town, Yingjiang County, Yunnan Province. Methods Anopheles mosquitoes were collected by light traps in Nabang Town, Yingjiang County, Yunnan Province in May 2014 and May 2021. After morphological identification, each mosquito was individually stored in separate tubes for further analysis. DNA of Anopheles minimus was extracted using kits. Microsatellite sequences in the template DNA were amplified using eight pairs of fluorescent primers, and the resulting products were subjected to capillary electrophoresis by a sequencing company. PopGen32 software was used to calculate the observed number of alleles (Na), effective number of alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), and Shannon's information index (I) for individual microsatellite loci and population groups. PIC-CALC software was used to calculate the polymorphic information content (PIC). Results A total of 158 mosquitoes belonging to 6 Anopheles species were captured in 2014, while 529 mosquitoes belonging to 5 Anopheles species were captured in 2021. The composition ratio of Anopheles minimus among the mosquito species differed significantly between 2014 and 2021 (χ2=70.48, P<0.01). For 8 microsatellite loci, a total of 85 alleles were detected, a range of 6-20 alleles per locus and an average of 10.625 alleles. Ne ranged from 1.717 to 7.797, with an average of 4.011. The highest PIC was found in the am4 locus, and the lowest in the am25 locus. For the population groups, 77 alleles were found in 2014, and 62 alleles were found in 2021. Ne ranged from 1.630 to 8.658, with an average of 4.147 in 2014. Ne ranged from 1.760 to 6.744, with an average of 3.698 in 2021. The average Ho was 0.641 in 2014 and 0.650 in 2021, while the average He was 0.699 in 2014 and 0.691 in 2021. The Shannon's index ranged from 0.774 to 2.493 in 2014, with an average of 1.579, and from 0.938 to 2.224 in 2021, with an average of 1.464. Na, Ne, I, and PIC were all higher in 2014 compared to 2021, with Na: 9.625>7.750, Ne: 4.147>3.698, I: 1.579>1.464, and PIC: 0.655>0.640, respectively. Conclusions The populations of Anopheles minimus in Nabang Town, Yingjiang County, exhibited high levels of polymorphism in both 2014 and 2021. However, the genetic diversity of the population in 2021 was lower than that in 2014.

Objective To investigate the-75 G/A single-nucleotide polymorphism in the promoter region of apolipoprotein A1 gene(apoA1)and its association with gestational diabetes mellitus(GDM)in pregnant women and to provide references for the exploration in the molecular genetic basis of GDM.Methods A total of 626 GDM patients and 1022 normal pregnant women,ie,the controls,were included in the study.The genotyping of apoA1-75 G/A polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP)analysis.Total cholesterol(TC),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),and glucose(Glu)were measured by enzymatic methods.Plasma insulin(INS)was measured by chemiluminescence immunoassay.The protein levels of apoA1 and apoB were measured by the turbidimetric immunoassay.Results Allele frequencies of G and A were 0.718 and 0.282 in the GDM group and 0.713 and 0.287 in the control group,respectively.Distribution of the genotype frequencies was found to be in Hardy-Weinberg equilibrium in both the GDM and control groups.There was no significant difference in the frequencies of alleles G and A and the genotypes of apoA1-75 G/A polymorphism between the GDM and the control group(P>0.05).In the GDM group,the carriers with the genotype AA were associated with significantly higher levels of TC,HDL-C,and apoA1 than those with genotypes GG and GA did(all P<0.05).After the GDM patients were divided into obese and non-obese subgroups,the genotype-related apoA1 variation was observed only in obese patients,while the genotype-related TC and HDL-C variations were evident in non-obese patients(P<0.05).In the control group,carriers of genotypes AA and GA had higher systolic blood pressure(SBP)and HDL-C than the carriers of genotype GG did(all P<0.05).Carriers of genotypes AA had significantly lower Glu levels than carriers of genotypes GG and GA did(P<0.05).The control subjects were further divided into subgroups according to their body mass index(BMI).Analysis of the subgroups showed that AA carriers were associated with higher SBP levels in the obese control women only,while lower Glu levels were evident in both obese and non-obese control women.Conclusion These results suggest that-75 G/A polymorphism in the apoA1 gene is not associated with GDM.However,the genetic variation is closed associated with the plasma apoA1,HDL-C,and TC levels in GDM patients and plasma HDL-C,Glu,and SBP levels in the control subjects.The apoA1 variant-associated lipids and SBP variation is BMI dependent in both groups.

Objective:To examine the expression of human leukocyte antigen G (HLA-G) in the peripheral blood and cancerous tissues of patients with papillary thyroid carcinoma (PTC) .Methods:The expression of soluble HLA-G (sHLA-G) in the peripheral blood of 50 individuals with PTC (PTC group) , 25 patients with benign thyroid tumors (BTT group) from Department of Thyroid and Breast Surgery, Beilun branch of the First Affiliated Hospital of Zhejiang University and 20 healthy controls (healthy control group) from physical examination center was assessed by ELISA. Immunohistochemical examination of HLA-G levels was also performed on tissue specimens from patients in the PTC and BTT groups, and their correlation with clinicopathological features of thyroid cancer was analyzed. SPSS 19.0 was used for statistical analysis. The measurement data of normal distribution were tested by two independent samples t test. Chi square test was used to compare the rates between the two groups. Results:The sHLA-G expression in peripheral blood was 21.33 (±5.54) , 22.73 (±4.99) , and 18.29 (±4.43) ng/mL in the preoperative PTC, BTT, and healthy control groups, respectively. Compared to the healthy group, sHLA-G levels were considerably higher in the PTC and BTT groups, with statistically significant differences (totally P < 0.05) . There was no significant difference in statistically sHLA-G levels between the BTT and PTC groups ( P > 0.05) . The positive HLA-G expression rate in PTC tissues was 78% (39/50) . There was no evidence of HLA-G expression in common tissues adjacent to PTC. HLA-G was not expressed in benign tumors. HLA-G was linked with the PTC tumor diameter, and the rate of positive expression was considerably greater with tumor diameters >1 cm than with those ≤1 cm ( P<0.05) . The rate of HLA-G positive expression was not significantly correlated with sex, age, multiple foci, extra-glandular invasion, metastasis of lymph nodes, or the TNM stage in PTC individuals ( P > 0.05) . Conclusions:HLA-G is significantly expressed at high levels in PTC tissues, is correlated with the tumor diameter, and may probably have a significant role in this disease. Peripheral blood sHLA-G may be associated with thyroid tumorigenesis, and its value in PTC requires further verification.

Objective:To explore the diagnostic value and clinical significance of total volume of quadratus femoris muscle (TQFMV), ischial angle, femoral neck angle (FNV) measured by magnetic resonance imaging (MRI) combined with eccentric distance and lesser trochanter height measured by multi-slice spiral CT (MSCT) in the diagnosis of ischiofemoral impingement (IFI) syndrome.Methods:A total of 82 patients with IFI in Beijing Huairou Hospital from October 2017 to July 2020 were selected as the observation group. In addition, 82 healthy patients who underwent MRI and MSCT were collected as the control group. The general data, MRI and MSCT parameters of the two groups were compared, and IFI influencing factors were analyzed by logistic regression. The correlation between MRI and MSCT parameters and clinical manifestations and the correlation between MRI and MSCT parameters were analyzed. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of MRI and MSCT parameters for IFI.Results:There were statistically significant difference between the two groups of gender, age, MRI parameters (TQFMV, ischial angle, FNV), and MSCT parameters (eccentricity, lesser trochanter height) (all P<0.05). Logistic regression analysis showed that gender, age, MRI parameters (TQFMV, ischial angle, FNV), MSCT parameters (eccentricity, lesser trochanter height ) were all influencing factors of IFI (all P<0.05). MRI parameters (TQFMV, ischial angle, FNV), MSCT parameters (eccentricity, lesser trochanter height) were all related to quadratus femoris muscle (QFM) edema, fat infiltration and pain degree in IFI patients (all P<0.05). The MRI parameter TQFMV of IFI patients was positively correlated with the MSCT parameter eccentricity and lesser trochanter height, while the ischial angle and FNV were negatively correlated with the MSCT parameter eccentricity and lesser trochanter height (all P<0.05). The AUC of MRI parameters (TQFMV, ischial angle, FNV) and MSCT parameters (eccentricity, lesser trochanter height) in the diagnosis of IFI were high, especially the highest in combined diagnosis, reaching 0.859. Conclusions:MRI parameters TQFMV, ischial angle, FNV and MSCT parameters, eccentricity and lesser trochanter height are related to the clinical manifestations of IFI patients. Combined detection of them can improve the diagnostic value of IFI and avoid missed diagnosis and misdiagnosis.

BACKGROUND: Physical therapy is regarded as an essential aspect in achieving optimal outcomes following total knee arthroplasty (TKA). The coronavirus disease 2019 (COVID-19) pandemic has made face-to-face rehabilitation inaccessible. Virtual reality (VR) is increasingly regarded as a potentially effective option for offering health care interventions. This systematic review and meta-analysis investigate VR-based rehabilitation's effectiveness on outcomes following TKA. METHODS: From inception to May 22, 2021, PubMed/Medline, Embase, Web of Science, the Cochrane Central Register of Controlled Trials, Scopus, PsycINFO, Physiotherapy Evidence Database, China National Knowledge Infrastructure, and Wanfang were comprehensively searched to identify randomized controlled trials (RCTs) evaluating the effect of VR-based rehabilitation on patients following TKA according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement and the Cochrane Handbook for Systematic Reviews of Interventions. RESULTS: Eight studies were included in the systematic review, and seven studies were included in the meta-analysis. VR-based rehabilitation significantly improved visual analog scale (VAS) scores within 1 month (standardized mean difference [SMD]: -0.44; 95% confidence interval [CI]: -0.79 to -0.08, P = 0.02), the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) within 1 month (SMD: -0.71; 95% CI: -1.03 to -0.40, P < 0.01), and the Hospital for Special Surgery Knee Score (HSS) within 1 month and between 2 months and 3 months (MD: 7.62; 95% CI: 5.77 to 9.47, P < 0.01; MD: 10.15; 95% CI: 8.03 to 12.27, P < 0.01; respectively) following TKA compared to conventional rehabilitation. No significant difference was found in terms of the Timed Up and Go (TUG) test. CONCLUSIONS VR-based rehabilitation improved pain and function but not postural control following TKA compared to conventional rehabilitation. More high-quality RCTs are needed to prove the advantage of VR-based rehabilitation. As the COVID-19 pandemic continues, it is necessary to promote this rehabilitation model.
Arthroplasty, Replacement, Knee ; COVID-19 ; Humans ; Randomized Controlled Trials as Topic ; SARS-CoV-2 ; Virtual Reality

Objective:To investigate the effect of ultrapulse fractional carbon dioxide laser (UFCL) microporous transdermal triamcinolone acetonide acetate (TAA) in the treatment of early hypertrophic scar.Methods:Ninety patients with early hypertrophic scar in Luoyang Central Hospital Affiliated to Zhengzhou University from March 2016 to March 2019 were included and randomly divided into study group ( n=30), control group A ( n=30) and control group B ( n=30). The control group A was treated with UFCL, the control group B was treated with TAA, and the study group was treated with UFCL combined with TAA. All three groups were treated for 6 months. The effectiveness of the three groups before and 6 months after treatment was compared, the status of hypertrophic scar was evaluated by Vancouver Scar Scale (VSS), the pain degree before and after treatment was evaluated by Visual Analogue Scale (VAS), and the patients’ satisfaction with the treatment was also evaluated. Results:(1) The study group and the control group were effective in the treatment of early hypertrophic scar, and the effective rate of the study group was higher than that of the control group A and the control group B [93.3%(28/30) vs. 70.0%(21/30) and 66.7%(20/30); all P<0.05]. (2) There was no significant difference in the scores of color, thickness, softness and vascular distribution among the three groups before treatment ( P>0.05). After 6 months of treatment, the scores of the three groups were lower than those before treatment ( P<0.05). The scores of study group were lower than those of control group A and control group B respectively( P<0.05). (3) There was no significant difference in VAS scores among the three groups before treatment ( P>0.05). The VAS scores of the three groups after treatment were lower than those before treatment, and the VAS scores in study group were lower than those in control group A and control group B( P<0.05). (4) After 6 months of treatment, the satisfaction rate of study group was higher than that of control group A and control group B ( P<0.05). Conclusions:UFCL microporous transdermal TAA can effectively treat early hypertrophic scar, reduce the VSS score and VAS score of hypertrophic scar, significantly improve the state of scar hyperplasia, and improve the satisfaction rate of patients, which can be widely used in clinic.

Objective:To investigate the effect of ultrapulse fractional carbon dioxide laser (UFCL) microporous transdermal triamcinolone acetonide acetate (TAA) in the treatment of early hypertrophic scar.Methods:Ninety patients with early hypertrophic scar in Luoyang Central Hospital Affiliated to Zhengzhou University from March 2016 to March 2019 were included and randomly divided into study group ( n=30), control group A ( n=30) and control group B ( n=30). The control group A was treated with UFCL, the control group B was treated with TAA, and the study group was treated with UFCL combined with TAA. All three groups were treated for 6 months. The effectiveness of the three groups before and 6 months after treatment was compared, the status of hypertrophic scar was evaluated by Vancouver Scar Scale (VSS), the pain degree before and after treatment was evaluated by Visual Analogue Scale (VAS), and the patients’ satisfaction with the treatment was also evaluated. Results:(1) The study group and the control group were effective in the treatment of early hypertrophic scar, and the effective rate of the study group was higher than that of the control group A and the control group B [93.3%(28/30) vs. 70.0%(21/30) and 66.7%(20/30); all P<0.05]. (2) There was no significant difference in the scores of color, thickness, softness and vascular distribution among the three groups before treatment ( P>0.05). After 6 months of treatment, the scores of the three groups were lower than those before treatment ( P<0.05). The scores of study group were lower than those of control group A and control group B respectively( P<0.05). (3) There was no significant difference in VAS scores among the three groups before treatment ( P>0.05). The VAS scores of the three groups after treatment were lower than those before treatment, and the VAS scores in study group were lower than those in control group A and control group B( P<0.05). (4) After 6 months of treatment, the satisfaction rate of study group was higher than that of control group A and control group B ( P<0.05). Conclusions:UFCL microporous transdermal TAA can effectively treat early hypertrophic scar, reduce the VSS score and VAS score of hypertrophic scar, significantly improve the state of scar hyperplasia, and improve the satisfaction rate of patients, which can be widely used in clinic.

OBJECTIVE: To assess the association of apolipoprotein (apo) C1 (APOC1) gene rs4420638A/G and -317H1/H2 polymorphisms with the risk of pre-eclampsia (PE) and the influence of their genotypes on the clinical and metabolic indexes among Chinese women. METHODS: In total 289 PE patients and 824 women with uncomplicated pregnancies were included. The rs4420638A/G genotype was determined by a Taqman real-time PCR allelic discrimination assay. The -317H1/H2 genotype was measured through PCR and restriction fragment length polymorphism analysis. Serum lipid and apo levels were measured by an enzymatic kit and a PEG-enhanced immunoturbidimetric assay. RESULTS: Allelic and genotypic frequencies of the APOC1 gene rs4420638A/G and -317H1/H2 were not significantly different between the two groups (all P> 0.05). However, patients carrying the G allele of the rs4420638A/G locus had higher serum levels of triglyceride, non-HDL-C and apoB, and a higher apoB/apoA1 ratio compared with those with an AA genotype (all P< 0.05). Patients carrying the H2 allele of the -317H1/H2 polymorphism had smaller delivery gestational weeks compared with those with the H1H1 genotype (P< 0.05). CONCLUSION Polymorphisms of the APOC1 gene rs4420638 and -317H1/H2 sites may be associated with abnormal lipoprotein metabolism among Chinese patients with PE, though no association was found between variants of the APOC1 gene and the risk of PE among them.

Singular periosseous osteochondroma-like hyperplasia is often misdiagnosed, because of its atypical skin lesion. The clinical reports in China are rare. On May 29, 2018, a patient with osteochondroma-like hyperplasia of singular bone on the right foot was diagnosed at the Department of Dermatology, First Affiliated Hospital of Zhengzhou University. Tissue biopsy was performed for histopathological confirmation. The incision healed well after operation, and there was no recurrence observed in the follow-up period of 5 months.