Objective: To enhance the recognition of necrotizing sialometaplasia (NS) by elucidating its clinical, pathological characteristics and key diagnostic points, providing a basis for the diagnosis and treatment of the disease. Methods: This study has been reviewed and approved by the Medical Ethics Committee, and informed consent has been obtained from patients. Review the data of a patient with NS occurring at the junction of the right soft and hard palate, and comprehensively analyze its diagnostic process based on its clinical manifestations, imaging, and histopathological examination results. And review the relevant literature on the disease. Results: This study describes a 24-year-old male patient with a documented betel nut habit (2 pieces/day for >6 months), who presented with a bone-deep, irregular crateriform ulcer (3 mm × 6 mm × 5 mm) localized to the right hard-soft palate junction. Spiral CT showed a local soft tissue defect with no apparent underlying bone destruction. Histopathology demonstrated chronic inflammation of the mucosal and minor salivary gland tissues, with no evidence of malignancy. A final diagnosis of NS was established. The ulcer healed completely three weeks after initiation of local anti-inflammatory therapy. A literature review indicates that NS is a rare, benign salivary gland disorder, typically occurring at the hard-soft palate junction in middle-aged men (40-60 years). Its etiology remains unclear, but it is widely attributed to salivary lobe infarction following mechanical trauma-induced ischemia. Due to its clinical resemblance to malignancy, it is often misdiagnosed. Treatment entails local anti-inflammatory measures and meticulous wound care aimed at promoting mucosal healing. Conclusion NS is a self-limiting, benign condition that poses a significant diagnostic challenge due to its close clinical simulation of malignancy. Thus, accurate diagnosis requires a combined assessment of clinical presentation, radiological features, and pathological findings. Treatment is predicated based on a conservative strategy with an emphasis on symptomatic management.

Radiochemotherapy-induced oral mucositis (OM) is a common oral complication in patients with tumors following head and neck radiotherapy or chemotherapy. Erosion and ulcers are the main features of OM that seriously affect the quality of life of patients and even the progress of tumor treatment. To date, differences in clinical prevention and treatment plans for OM have been noted among doctors of various specialties, which has increased the uncertainty of treatment effects. On the basis of current research evidence, this expert consensus outlines risk factors, clinical manifestations, clinical grading, ancillary examinations, diagnostic basis, prevention and treatment strategies and efficacy indicators for OM. In addition to strategies such as basic oral care, anti-inflammatory and analgesic agents, anti-infective agents, pro-healing agents, and photobiotherapy recommended in previous guidelines, we also emphasize the role of traditional Chinese medicine in OM prevention and treatment. This expert consensus aims to provide references and guidance for dental physicians and oncologists in formulating strategies for OM prevention, diagnosis, and treatment, standardizing clinical practice, reducing OM occurrence, promoting healing, and improving the quality of life of patients.
Humans ; Chemoradiotherapy/adverse effects* ; Consensus ; Risk Factors ; Stomatitis/etiology*

Bone grafting is a primary method for treating bone defects. Among various graft materials, xenogeneic bone substitutes are widely used in clinical practice due to their abundant sources, convenient processing and storage, and avoidance of secondary surgeries. With the advancement of domestic production and the limitations of imported products, an increasing number of bone filling or grafting substitute materials isentering clinical trials. Relevant experts have drafted this consensus to enhance the management of medical device clinical trials, protect the rights of participants, and ensure the scientific and effective execution of trials. It summarizes clinical experience in aspects, such as design principles, participant inclusion/exclusion criteria, observation periods, efficacy evaluation metrics, safety assessment indicators, and quality control, to provide guidance for professionals in the field.
Humans ; Bone Substitutes/therapeutic use* ; Randomized Controlled Trials as Topic/methods* ; Consensus ; Bone Transplantation ; Research Design

Objective:To explore the differences in bacterial communities within tissues during the process of oral mucosal carcinogenesis, and analyze the relationship between the high-abundance species Prevotella intermedia (Pi) and the occurrence and development of oral mucosal carcinogenesis. Methods:Fresh tissue samples were collected from patients diagnosed with oral leukoplakia (OLK), oral squamous cell carcinoma (OSCC), and healthy controls (HC) at Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University, from January 2022 to November 2024, following strict inclusion criteria. Bacterial DNA was extracted from these specimens, and the 2bRAD sequencing for microbiome (2bRAD-M) was employed to analyze and compare the α and β diversity, as well as the community composition of bacteria within tissues, aiming to identify specifically expressed bacteria. Subsequently, paraffin-embedded clinical specimens were collected: 15 cases in the OLK group (including 4 cases of simple hyperplasia, 6 cases of mild dysplasia, and 5 cases of moderate to severe dysplasia), 12 cases in the OSCC group, and 5 cases in the HC group. A 4-nitroquinoline N-oxide (4NQO)-induced OLK progression mouse model was also constructed. Mice were randomly divided into three groups using a random number table, with six in each group. The negative control group was given distilled water to drink; Group 1 was given distilled water containing 4NQO to drink until week 12, while Group 2 was given distilled water containing 4NQO to drink until week 22. After the mice were sacrificed, their tongue tissue were collected and fixed. Fluorescence in situ hybridization (FISH) with specific probes was used to validate the presence of Pi in human and mouse tissue sections, analyzing the correlation between histopathological grading and the invasion depth of Pi.Results:The 2bRAD-M microbial analysis revealed that the relative abundance of Pi in OSCC tissues (10.80%) was significantly higher than in the HC group (0.50%) ( P=0.001) and OLK group (0.70%) ( P=0.002). FISH probe detection showed that the fluorescence intensity of Pi in human OSCC tissues [123.50 (101.00, 142.30)] was higher than in the HC group [0.00 (0.00, 28.50)], simple hyperplasia OLK [0.00 (0.00, 35.25)], and mild dysplasia OLK [24.50 (0.00, 55.50)] groups, with statistically significant differences respectively ( P=0.002, P=0.003, P=0.005). However, there was no significant difference compared to moderate to severe dysplasia OLK [56.00 (28.00, 62.50)] ( P=0.210). The fluorescence area of Pi in human OSCC tissues [8 615.00 (7 439.00, 11 084.00)] was significantly larger than in the HC group [0.00 (0. 00, 45.00)], simple hyperplasia OLK group [0.00 (0.00, 81.00)], mild dysplasia [49.00 (0.00, 151.00)], and moderate to severe dysplasia groups [1 450.00 (454.00, 2 892.00)], with highly significant differences ( P<0.001). There was a significant correlation between the invasive depth of Pi and the degree of histopathological grading ( P<0.001). In mice, the fluorescence intensity of Pi in OSCC tissues [120.00 (110.00, 127.00)] was significantly higher than in the HC group [0.00 (0.00, 12.25)] ( P<0.01), but showed no significant difference compared with the OLK group [50.00 (0.00, 58.00)] ( P>0.05). The fluorescence area of Pi in mice OSCC tissues [11 020.00 (6 790.00, 12 102.00)] was significantly larger than in the HC group [0.00 (0.00, 56.75)] and the OLK group [0.00 (0.00, 751.50)] ( P=0.006, P=0.043). There is a significant correlation between the depth of invasion of Pi and the degree of histopathological grading ( P<0.01). Conclusions:This study suggests that Pi in oral mucosal tissue may be a potential biomarker for early detection of OSCC and play an important role in the carcinogenesis process of oral mucosa.

Objective:Analyzing the overview, changing trends, hotspots, and frontiers of the rare oral disease Laugier-Hunziker syndrome (LHS) using bibliometrics.Methods:Search the CNKI (China National Knowledge Infrastructure) and Web of science core collection (WOSCC) databases. The time span for Chinese literature is from January 1992 to December 2023, and for English literature is from January 1986 to December 2023. Utilize Citespace software to conduct visualized analysis on various aspects of the literature, including publication volume, authors, countries, institutions, journals, and keywords.Results:This study included a total of 112 articles (25 in Chinese and 87 in English). The overall publication volume showed a fluctuating slow-growth trend. Foreign authors Lenane P and Powell F had the highest number of publications. The team from Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, had the most publications among domestic authors. In terms of international collaboration, the United States demonstrated the highest centrality, but the collaborative network worldwide appeared relatively loose. University College Dublin was the foreign institution with the highest publication volume, while Peking Union Medical College Hospital was the domestic institution with the most publications. The journal with the highest number of foreign publications was the Journal of The American Academy of Dermatology, and the journal with the most domestic publications was the Chinese Leprosy Skin Disease Journal. Research hotspots for Laugier-Hunziker syndrome included lesion sites and differential diagnosis, while the frontiers of research encompassed differential diagnosis, disease management, and clinical manifestations related to oral mucosa. Conclusions:Laugier-Hunziker syndrome research shows a focus on differential diagnosis and symptom management. Future studies should prioritize optimizing treatment methods and emphasizing psychological support, while promoting interdisciplinary collaboration and the integration of global research networks to improve clinical diagnostic and therapeutic outcomes.

Objective To investigate the effect of lysosome biogenesis complex 1 subunit 1(GCN5L1)on myocardial inflammatory injury and regulatory T cell(Treg)infiltration in mice with myocardial infarction(MI).Methods The differentially expressed gene GCN5L1 was screened from the GSE66360 dataset using whole blood samples from healthy controls and MI patients.The correlation between GCN5L1 and IL-10,IL-35 or Treg marker FOXP3 was analyzed.A model of MI was established in C57BL/6 mice by left anterior descending coronary artery ligation.Thirty mice were divided into five groups:sham,MI model,AAV9-NC(control adeno-associated virus),AAV9-GCN5L1 and AAV9-GCN5L1+FOXP3 inhibitor(epirubicin)groups,with 6 mice in each group.MI area was assessed by TTC staining,and histopathological changes were evaluated via HE staining.Immunofluorescence was used to quantify GCN5L1 and FOXP3 positivity;ELISA was used to measure IL-10 and IL-35 levels;Western blot was used to analyze the levels of GCN5L1,Bax,Bcl-2 and PI3K/AKT pathway proteins;transcriptomic sequencing was performed on AAV9-NC and AAV9-GCN5L1 groups,while differential genes subjected to GO and KEGG enrichment analysis.Results GSE66360 dataset showed that GCN5L1 levels were significantly lower in MI patients than in healthy controls,and positively correlated with IL-10,IL-35 and FOXP3 levels(P＜0.01).Compared to the sham group,the MI group exhibited increased infarct area,severe histopathology,reduced GCN5L1/IL-10/IL-35 levels,elevated Bax,and decreased Bcl-2,which could be reversed by AAV9-GCN5L1(P＜0.01).Transcriptomics revealed the enrichment of differential genes in Treg infiltration and PI3K/AKT pathways.The AAV9-GCN5L1 group showed higher FOXP3 positivity and PI3K/AKT expression than the MI group.Co-treatment with AAV9-GCN5L1+epirubicin reduced FOXP3 positivity compared to AAV9-GCN5L1 alone(P＜0.01).Conclusion GCN5L1 overexpression alleviates myocardial inflammatory injury post-MI,activates the PI3K/AKT pathway,and enhances Treg infiltration.

Objective To investigate the effect of lysosome biogenesis complex 1 subunit 1(GCN5L1)on myocardial inflammatory injury and regulatory T cell(Treg)infiltration in mice with myocardial infarction(MI).Methods The differentially expressed gene GCN5L1 was screened from the GSE66360 dataset using whole blood samples from healthy controls and MI patients.The correlation between GCN5L1 and IL-10,IL-35 or Treg marker FOXP3 was analyzed.A model of MI was established in C57BL/6 mice by left anterior descending coronary artery ligation.Thirty mice were divided into five groups:sham,MI model,AAV9-NC(control adeno-associated virus),AAV9-GCN5L1 and AAV9-GCN5L1+FOXP3 inhibitor(epirubicin)groups,with 6 mice in each group.MI area was assessed by TTC staining,and histopathological changes were evaluated via HE staining.Immunofluorescence was used to quantify GCN5L1 and FOXP3 positivity;ELISA was used to measure IL-10 and IL-35 levels;Western blot was used to analyze the levels of GCN5L1,Bax,Bcl-2 and PI3K/AKT pathway proteins;transcriptomic sequencing was performed on AAV9-NC and AAV9-GCN5L1 groups,while differential genes subjected to GO and KEGG enrichment analysis.Results GSE66360 dataset showed that GCN5L1 levels were significantly lower in MI patients than in healthy controls,and positively correlated with IL-10,IL-35 and FOXP3 levels(P＜0.01).Compared to the sham group,the MI group exhibited increased infarct area,severe histopathology,reduced GCN5L1/IL-10/IL-35 levels,elevated Bax,and decreased Bcl-2,which could be reversed by AAV9-GCN5L1(P＜0.01).Transcriptomics revealed the enrichment of differential genes in Treg infiltration and PI3K/AKT pathways.The AAV9-GCN5L1 group showed higher FOXP3 positivity and PI3K/AKT expression than the MI group.Co-treatment with AAV9-GCN5L1+epirubicin reduced FOXP3 positivity compared to AAV9-GCN5L1 alone(P＜0.01).Conclusion GCN5L1 overexpression alleviates myocardial inflammatory injury post-MI,activates the PI3K/AKT pathway,and enhances Treg infiltration.

Objective:To explore the differences in bacterial communities within tissues during the process of oral mucosal carcinogenesis, and analyze the relationship between the high-abundance species Prevotella intermedia (Pi) and the occurrence and development of oral mucosal carcinogenesis. Methods:Fresh tissue samples were collected from patients diagnosed with oral leukoplakia (OLK), oral squamous cell carcinoma (OSCC), and healthy controls (HC) at Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University, from January 2022 to November 2024, following strict inclusion criteria. Bacterial DNA was extracted from these specimens, and the 2bRAD sequencing for microbiome (2bRAD-M) was employed to analyze and compare the α and β diversity, as well as the community composition of bacteria within tissues, aiming to identify specifically expressed bacteria. Subsequently, paraffin-embedded clinical specimens were collected: 15 cases in the OLK group (including 4 cases of simple hyperplasia, 6 cases of mild dysplasia, and 5 cases of moderate to severe dysplasia), 12 cases in the OSCC group, and 5 cases in the HC group. A 4-nitroquinoline N-oxide (4NQO)-induced OLK progression mouse model was also constructed. Mice were randomly divided into three groups using a random number table, with six in each group. The negative control group was given distilled water to drink; Group 1 was given distilled water containing 4NQO to drink until week 12, while Group 2 was given distilled water containing 4NQO to drink until week 22. After the mice were sacrificed, their tongue tissue were collected and fixed. Fluorescence in situ hybridization (FISH) with specific probes was used to validate the presence of Pi in human and mouse tissue sections, analyzing the correlation between histopathological grading and the invasion depth of Pi.Results:The 2bRAD-M microbial analysis revealed that the relative abundance of Pi in OSCC tissues (10.80%) was significantly higher than in the HC group (0.50%) ( P=0.001) and OLK group (0.70%) ( P=0.002). FISH probe detection showed that the fluorescence intensity of Pi in human OSCC tissues [123.50 (101.00, 142.30)] was higher than in the HC group [0.00 (0.00, 28.50)], simple hyperplasia OLK [0.00 (0.00, 35.25)], and mild dysplasia OLK [24.50 (0.00, 55.50)] groups, with statistically significant differences respectively ( P=0.002, P=0.003, P=0.005). However, there was no significant difference compared to moderate to severe dysplasia OLK [56.00 (28.00, 62.50)] ( P=0.210). The fluorescence area of Pi in human OSCC tissues [8 615.00 (7 439.00, 11 084.00)] was significantly larger than in the HC group [0.00 (0. 00, 45.00)], simple hyperplasia OLK group [0.00 (0.00, 81.00)], mild dysplasia [49.00 (0.00, 151.00)], and moderate to severe dysplasia groups [1 450.00 (454.00, 2 892.00)], with highly significant differences ( P<0.001). There was a significant correlation between the invasive depth of Pi and the degree of histopathological grading ( P<0.001). In mice, the fluorescence intensity of Pi in OSCC tissues [120.00 (110.00, 127.00)] was significantly higher than in the HC group [0.00 (0.00, 12.25)] ( P<0.01), but showed no significant difference compared with the OLK group [50.00 (0.00, 58.00)] ( P>0.05). The fluorescence area of Pi in mice OSCC tissues [11 020.00 (6 790.00, 12 102.00)] was significantly larger than in the HC group [0.00 (0.00, 56.75)] and the OLK group [0.00 (0.00, 751.50)] ( P=0.006, P=0.043). There is a significant correlation between the depth of invasion of Pi and the degree of histopathological grading ( P<0.01). Conclusions:This study suggests that Pi in oral mucosal tissue may be a potential biomarker for early detection of OSCC and play an important role in the carcinogenesis process of oral mucosa.

Objective:Analyzing the overview, changing trends, hotspots, and frontiers of the rare oral disease Laugier-Hunziker syndrome (LHS) using bibliometrics.Methods:Search the CNKI (China National Knowledge Infrastructure) and Web of science core collection (WOSCC) databases. The time span for Chinese literature is from January 1992 to December 2023, and for English literature is from January 1986 to December 2023. Utilize Citespace software to conduct visualized analysis on various aspects of the literature, including publication volume, authors, countries, institutions, journals, and keywords.Results:This study included a total of 112 articles (25 in Chinese and 87 in English). The overall publication volume showed a fluctuating slow-growth trend. Foreign authors Lenane P and Powell F had the highest number of publications. The team from Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, had the most publications among domestic authors. In terms of international collaboration, the United States demonstrated the highest centrality, but the collaborative network worldwide appeared relatively loose. University College Dublin was the foreign institution with the highest publication volume, while Peking Union Medical College Hospital was the domestic institution with the most publications. The journal with the highest number of foreign publications was the Journal of The American Academy of Dermatology, and the journal with the most domestic publications was the Chinese Leprosy Skin Disease Journal. Research hotspots for Laugier-Hunziker syndrome included lesion sites and differential diagnosis, while the frontiers of research encompassed differential diagnosis, disease management, and clinical manifestations related to oral mucosa. Conclusions:Laugier-Hunziker syndrome research shows a focus on differential diagnosis and symptom management. Future studies should prioritize optimizing treatment methods and emphasizing psychological support, while promoting interdisciplinary collaboration and the integration of global research networks to improve clinical diagnostic and therapeutic outcomes.

Objective:To analyze the influencing factors and improvement paths of the cultivation of full-time doctoral scientific and technological innovation ability in large public hospitals, and propose countermeasures and suggestions.Methods:This studyed conducted a survey and analysis of 122 doctors from Linyi People′s Hospital in Shandong Province, and completed a current situation study based on the analysis results.Results:There was no significant difference between the two groups in gender, age, degree type, professional category, discipline level, Graduate School type, job type and other indicators. There were significant differences between the two groups in scientific research topic selection ability score, project design ability score, data analysis ability score, data interpretation ability score, project approval in recent 5 years, project level, number of SCI journal papers published in recent 5 years, cumulative impact factors of SCI journal papers, and annual number of academic activities ( P<0.05). Conclusions:The hospital can improve the scientific and technological innovation ability of full-time doctors by setting up a special cultivation plan, establishing an interdisciplinary team, optimizing scientific research management services, improving the evaluation and assessment system, and improving welfare protection.