Background: Skin wound healing, elasticity, and protective functions are closely associated with cellular regeneration and the remodeling of the extracellular matrix (ECM). MXRA7 (Matrix Remodeling Associated Protein 7) is a protein involved in ECM regulation, tissue regeneration, and inflammatory processes, and recent studies suggest that it may play a supportive role in skin wound healing. However, systematic investigations on natural small molecules capable of modulating MXRA7 activity remain limited. Flavonoids, on the other hand, have been reported to promote wound healing and ECM regulation through their antioxidant and anti-inflammatory properties. Therefore, it is hypothesized that flavonoid compounds may act as potential modulators of MXRA7 protein activity. Aim: The aim of this study was to evaluate the binding affinity, binding energy, and interaction characteristics of 14 naturally occurring flavonoid compounds with the active site of the MXRA7 protein using a molecular docking approach. Materials and Methods: The molecular structures of the flavonoids were obtained from the PubChem database, and their interactions with the MXRA7 protein were investigated using molecular docking with CB-Dock2 and SwissDock software. Docking results were visualized using PyMOL and evaluated based on root mean square deviation (RMSD), binding free energy (∆G), inhibition constant (Ki), pose ranking, and cluster analysis. An RMSD value of ≤ 2.0 Å was considered indicative of a stable binding pose. Result: The RMSD values of the MXRA7–flavonoid complexes ranged from 0.081 to 0.622 Å, indicating that all compounds stably occupied the binding cavity of the protein. Fisetin (0.081 Å), naringenin (0.107 Å), and hesperetin (0.107 Å) exhibited the lowest RMSD values, suggesting highly stable binding poses. The binding free energy (∆G) values ranged from −6.0 to −7.1 kcal/mol, with protopine showing the strongest binding affinity (∆G=−7.1 kcal/mol). Most flavonoids formed hydrogen bonds, hydrophobic interactions, and π–π interactions with key amino acid residues of MXRA7. Cluster analysis revealed that the majority of flavonoids shared a common binding mode, while fisetin, naringenin, and hesperetin formed a distinct subcluster with more stable binding poses. Ligand efficiency (LE) analysis indicated that chrysin, apigenin, and naringenin exhibited higher LE values, reflecting greater binding efficiency of smaller molecules. Conclusion This study demonstrates, through molecular docking analysis, that flavonoid compounds can stably interact with the active site of the MXRA7 protein. Protopine showed the strongest binding energy, whereas fisetin, naringenin, and hesperetin exhibited superior binding pose stability. These compounds may serve as potential bioactive molecules targeting MXRA7; however, further validation through molecular dynamics simulations and experimental studies is required.

Background: Respiratory viral and bacterial infections are one of the leading causes of hospitalization and death worldwide, particularly affecting children, the elderly, and immune compromised individuals. The detection of causative pathogens is crucial for understanding the epidemiology, prevention, management, and treatment of severe pneumonia. Molecular diagnostic methods provide high sensitivity and speed, enabling the simultaneous detection of viral and bacterial co-infections. Aim: Detection of single and combined viral and bacterial infections in hospitalized children with pneumonia using multiplex PCR. Materials and Methods: We conducted a cross-sectional study design involving 100 hospitalized participants at the National Center for Mothers, Newborns, and Women II. The study utilized questionnaires and laboratory analysis methods. Nasopharyngeal swabs were collected from the participants using a sterile swab and transported in a UTM transport medium (Jiangsu, China). After nucleic acid extraction using the STARMag 96 ProPrepC assay, the samples were analyzed with the Seeprep 32 automated analyzer (Seegene Inc., Korea). Real-time polymerase chain reaction (PCR) analysis was performed using the Allplex™ RV Master Assay and Allplex™ PneumoBacter Assay (Seegene Inc., Korea), with the CFX-96 system (BioRad Inc., USA). Results: The prevalence of viral infections in the study population was as follows: influenza type A 3% (n=3), influenza type B 3% (n=3), total influenza virus 14% (n=14), respiratory syncytial virus 18% (n=18), adenovirus 10% (n=10), rhinovirus 20% (n=20), and SARS-CoV-2 5% (n=5). No bacterial infections, such as B.parapertussis or L.pneumophila, were detected. However, bacterial infections identified included B.pertussis in 1% (n=1), C.pneumoniae in 2% (n=2), H.influenzae in 43% (n=43), M.pneumoniae in 52% (n=52), and S.pneumoniae in 45% (n=45). Of the 100 children in the study, 6% had influenza or influenza-like illness. Among these, 43% had the virus alone, and 14% had a viral co-infection. Bacterial infections were detected individually in 40% of cases, with co-infections in 46% of bacterial cases. Conclusion Metapneumovirus, B.parapertussis, and Legionella pneumophila were not detected in the study participants (0%). Among the viral infections, respiratory syncytial virus (18%, n=18) and rhinovirus (20%, n=20) had the highest prevalence. For bacterial infections, H.influenzae (43%, n=43), M.pneumoniae (52%, n=52), and S.pneumoniae (45%, n=45) were most commonly detected.

Background: Erkhem-8 medicine is a composed Crocus Sativus L, Lagotis integrifolia, Gentiana barbata froel etc, used for the treatment of respiratory diseases, specially pneumonia and fever or hot disease in Mongolian Traditional Medicine. Aim: The effects of Erkhem-8 medicine to determine the some sourcebook study results. Materials and Methods: An evaluation of its effects on wind, bile, and phlegm was conducted using Traditional Mongolian Medicine methods (Dagvatseren B, 1996). In order to clarify the structure and composition of Erkhem-8 traditional medicines, and the treatment of lung fever from traditional medical sources. Text and source-study and comparison method were used in the study. Results: The study found that Erkhem-8 medicine demonstrated a varying capacity to suppress wind, bile, and phlegm, with bile suppression being the most significant at 0.68, followed by wind suppression at 0.58, and phlegm suppression at 0.16. Erkhem-8 is composed of 8 ingredients, which are safflower Carthamus tinctorius L, gmeline Bostaurus domesticus, Schizostachoum chinense Rendle, wood Santalum album L, Odontitis rubra Baung.Opiz, flora Gentiana decumbens L, herba Lagoti sintegrifolia Schischk, flora Aconitum Kusnezoffii Reichenb. These herbs themselves have a variety of biological activities, including reduce heat, pain relief, anti-oxidant and anti-tumor, anti-inflammation activity. Conclusions 1. The study found that Erkhem-8 exhibited the highest suppression of bile (0.68), followed by wind (0.58), and phlegm (0.16), indicating its effectiveness in modulating these elements. 2. Most of the raw materials included in the composition of Erkhem-8 medicine have a bitter and sweet taste and have the ability to remove heat, especially heat of the solid organs, i.e. heat of the lungs, liver, blood, specially pneumonia and fever or hot disease in Traditional Mongolian Medicine.

To detect factors influencing infant’s weight growth during the last few years and to do comparing analysis between mother’s and infant’s weight. 3600 birth histories were chosen and used out of all childbirths happened in 2010-2014 in Ulaanbaatar’s First Maternity hospital using random sampling method. Questionnaires consisting of 21 questions were performed within primiparas, mothers giving birth not for the first time and mothers giving birth by cesarean section. Processing of all the workings were done by using Microsoft Word 8 and Microsoft Excel 2007.According to the results of the research there is a tendency to weight growth of the infants. Comparing weights of the first child of the woman (women giving birth repeatedly and women giving birth by cesarean section) with the second one there is an increasing number of infants with heavier weight. And also as per research done within primiparas there is an increasing number of children born weighting more than 4010 grams during last 5 years. To compare heights of the women giving birth, there is a dominating number of women whose heights are between 155-160,9. However, there is a tendency of birth rate to increase in women whose heights are 161cm. According to the research 63% of all women giving birth were 21-30 years old, average age of women is 28,1 years old. We also detected that there is a dominating number of women giving birth for the 2nd time, but also we concluded that there is an increase in a frequency of women giving birth for the 3rd and 4th times as well. Birth frequency\5 years average\: according to the research we have done we are concluding that 46% of all women are giving birth for the 2nd time, 48%- for the 3rd time, 5% of all women are giving birth for the 4th time and 1% of them are having their 5th child. Significants of cesarean section: 338 are repeated cesarean section, 228 are chronic hypoxia, 131are preeclampsia, beech presentation are171 and other. Childbearing time comparing there is an increase in childbearing of 40-41 weeks.. Comparison between first child’s weight and second child’s weight. As per comparison done between a women’s previous child’s weight and a present child’s weights there is a decrease in a number births of children weights of whose are between 3000-3500 g of 8%, and also an increase in number of births of the children weighting 3510-4000 g by 9,2%, of the children weighting 4010-4500 g by 3% and of the children weighting more than 4510 g by 1,4% was seen. Compare between weights of the first child and second child of the women giving birth by cesarean section done between first and second children’s weights we detected that there is an increase in a number of children weighting 3000-3500 g by 10,4%, weighting 3510-4000 g by 10,2%, weighting 4010-4500 by 12,8% and weighting more than 4510 by 2,59%. Women’s first child’s weight comparison are showing there is a dominating number of children born weighting 3000-4000 grams, however, we also see that there is a slightly increase in number of children born weighting more than 4010 grams. As per research we have done between average heights of the women giving birth in a last 5 years we are concluding following: height of 13% of all women who were involved in a research was 149-154 cm, 39%’s height was 155-160,9 cm, 27%- 161- 166,9 cm and 19% of them were taller than 167cm. According to the research we see dominating number of over weighted women. Despite of sampling method not showing good enough picture of weight index increase we can conclude that number of 2r and 3r levels of overweight mothers are not only staying at the same level but also has some tendency to grow. It was observed that the fact of weight gaining, bad eating habits, not doing sports during pregnancy and increasing of women height acceleration and post-term pregnancy are influencing infant’s weight gain which leads to infant’s morbidity and pathology and to complications during pregnancy and childbirth.When women give birth repeatedly with every birth they give there is an increase in a child’s weight. And also between primiparas there is an increased number of children born weighting more than 4010 grams.Between women involved in a research there is a dominating number of women who are 155-160,9 cm tall, however, it is 46% of them whose heights are more than 161 cm. It shows that average height of Mongolian women is increasing.According to weight index not only women with overweight are dominating, but also number of women with 2 and 3r levels of overweight never goes down and slightly increases. From this we may conclude that overweight problems of women are influencing weight of the baby and also contributes to childbirth and pregnancy complications.