Background: Cisplatin is a widely used platinum-based chemotherapeutic agent, but its gonadotoxic effects can damage ovarian tissue, oocytes, and developing follicles and may lead to primary ovarian insufficiency (POI). Adipose-derived stem cells (ADSCs) are being investigated as a potential regenerative treatment because of their paracrine, anti-apoptotic, and tissue-repair effects. Aim: To evaluate the effect of ADSC therapy on estrous cyclicity, body weight, and ovarian follicular morphology in a mouse model of cisplatin-induced POI. Materials and Methods: Thirty 6-week-old female BALB/c mice were included. Six mice received saline as controls, while 24 mice received cisplatin 2 mg/kg intraperitoneally once daily for 10 days to induce POI. After POI induction, cisplatin-exposed animals were continued as an untreated POI group (n=15) or an ADSC-treatment group (n=9). The ADSC group received 1×10⁶ cells suspended in 0.2 mL medium via the tail vein. Body weight and vaginal cytology were monitored daily, and estrous stages were classified as proestrus, estrus, metestrus, and diestrus. Ovaries were collected for histologic evaluation at the end of the study. Result: After cisplatin exposure, mean estrous cycle length increased from 3.97 days in controls to 6.4 days in the POI group (p<0.05). During the post-treatment evaluation, mean cycle length was 5.65 days in untreated POI mice (n=4) and 4.5 days in ADSC-treated mice (n=7), representing a statistically significant improvement after ADSC therapy (p=0.013). Cisplatin caused significant body-weight loss, reaching a mean of 18.0 g on day 6 after completion of cisplatin administration (p<0.01). Histologically, untreated POI ovaries showed follicular atrophy and prominent degenerative changes, whereas ADSC-treated ovaries showed more morphologically preserved primary, secondary, and antral follicles and relatively fewer degenerative changes. Exploratory manual counting in three representative whole-ovary sections per group showed total intact/growing follicle counts of 15.00±2.65 in controls, 6.00±2.00 in POI, and 8.67±2.89 in ADSC-treated ovaries. Conclusion ADSC treatment was associated with partial restoration of estrous cyclicity and ovarian follicular development in a cisplatin-induced POI mouse model. These findings support further investigation of ADSC-based regenerative therapy for chemotherapy-associated ovarian injury.

BackgroundSurgery correction is necessarily required for complete cure of congenital and acquired heart diseases. Introduction of extracorporeal circulation method into practice has allowed to correct small sized atrial septal defects, as well as to compensate large atrial septal defects with synthetic organ or pericardium. The study on acid-base balance during the cardiac correction with extracorporeal circulation to treat heart disorders will allow to control and stabilize acid-base balance, and ensure the normal functioning of organism during the operation. Subsequently, it may serve as basic information for prevention from complications during and after surgery.Materials and MethodsTotally 75 patients who are subjected to cardiac correction of atrial septals defects were involved in the survey. Blood samples were taken in 3 stages (at the beginning, in the process of surgery and at the end) since extracorporeal circulation was established, and subsequently it was determined blood acid-base (ðÍ), blood carbon partial pressure (ÐÑÎ2), blood oxygen partial pressure (ÐÎ2), base excess (ÂÅ) and blood base bicarbonate (ÍÑÎ3).ResultsThe amount of pH and pCO2 contained in blood during the extracorporeal circulation are most likely (p<0.001) differed statistically at the beginning, in the process, and at the ending part. At the beginning and during the process of correction there were revealed sub-compensated alkalosis whereas at the end of procedure there was sub-compensated acidosis. Also under influence of body temperature, the pH and pCO2 are statistically changed.Conclusions:1. Even pH was achieving in normal range before stopping the extracorporeal circulation, there has demand to rearranging system because of there has acidosis and alkalosis are coming out. 2. The buffer system such as pCO2 and BE are working for repairing the repiratory and metabolic alkalosis and acidosis by holding pH in normal range while at the beginning, in the process and at the end in stages of extracorporeal circulation.